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Citations for Tocris :
451 - 500 of 546 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Parental A549 cells were fixed with 4% paraformaldehyde following 3-hour treatment with poly I:C (2.0 μg/mL, Tocris), LPS (1.0 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... to create a single cell suspension prior to resuspension in TeSR medium supplemented with ROCK kinase inhibitor (Tocris 1254 ...
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bioRxiv - Genomics 2023Quote: ... and seeded at a seeding density of 10,000–25,000 cells/cm2 in mTeSR supplemented with 2 μM ROCK inhibitor Y27632 (Tocris) onto laminin-521 coated plates ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 mL fresh RPMI 1640 containing B-27 supplement minus insulin was added to 0.5 mL conditioned (old) medium and added to cells along with 5 µM IWP2 (Tocris). Medium was aspirated at Day 5 and replenished with fresh RPMI 1640 containing B-27 supplement minus insulin ...
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bioRxiv - Cell Biology 2020Quote: ... Electroporated cells were seeded in fresh E8 media containing 5 μM Y-27632 dihydrochloride (a selective ROCK inhibitor, Tocris) and allowed to recover for 48 hours before antibiotic selection.
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bioRxiv - Developmental Biology 2020Quote: ... and seeding onto fresh MEFs with 10 μM Y27632 ROCK inhibitor overnight to increase cell survival (Tocris Bioscience, UK).
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bioRxiv - Molecular Biology 2021Quote: ... cells were pre-treated with 10 μM of Poly (ADP-ribose) glycohydrolase inhibitor (PARGi, TOCRIS Bio-Techne, PDD 00017273) for 30 mins prior to IR exposure ...
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bioRxiv - Neuroscience 2021Quote: ... Where indicated cells were pre-incubated with a nitric oxide donor (S)-Nitroso-N-acetylpenicillamine (SNAP, 1000 μM, Tocris) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Whole-cell voltage-clamp recordings for mEPSC and mIPSC were conducted in the presence of TTX (1 μM; Tocris) and Picrotoxin (100 uM ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in E8 medium supplemented with 10 µM ROCK inhibitor Y-27632 dihydrochloride (Tocris, Bristol, United Kingdom) and seeded on Matrigel-coated 12-well plates at a density of (1.5–2.5)×104 cells/cm2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Depletion of endogenous proteins fused to the 2xHA-FKBP12F36V domain (dTAG) was achieved by treating the cells with 0.5 μM dTAG-13 compound (Tocris, 1:1000 dilution of 0.5 mM stock solution in DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... hESCs were replated onto 35 mm Matrigel-coated plates at a density of 1.5 x 105 cells/cm2 in E8 medium with 10 μM ROCK inhibitor (Y27632, Tocris). The media was replaced with Essential 6 (E6 ...
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bioRxiv - Cancer Biology 2020Quote: ... 70% confluent transduced cells were treated with 1μM of the positive CaSR modulator NPS R-568 (Tocris Bioscience, UK) or the negative CaSR modulator NPS 2143 (Tocris Bioscience ...
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bioRxiv - Bioengineering 2019Quote: ... cells were re-plated onto Matrigel-coated plates at a density of 2.5×105 cells/cm2 in E8 medium containing 10 μM Y27632 (Tocris). The following day ...
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bioRxiv - Developmental Biology 2020Quote: ... day 3 NMP-like cell cultures were treated with Accutase and replated at a density of 63,000 cells//cm2 in N2B27 supplemented with CHIR99021 (3 µM, Tocris), FGF2 (100 ng/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then differentiated for 3 days (D) in N2B27 supplemented with 3 µM Chiron99021 (Tocris, Cat. No. 4953) and 20ng/ml bFGF (PeproTech ...
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bioRxiv - Genetics 2021Quote: ... Cells were then incubated in RPMI/B27 (-Insulin) for 24 hours before changing media to 5 μM IWP2 (Tocris) in RPMI/B27(-Insulin) ...
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bioRxiv - Cell Biology 2021Quote: ... organoids cultured for 8 days with airway cell culture medium were followed by inclusion of CHIR99021 (2 µM, Tocris) for additional 10 days with or without DAPT (20 µM) ...
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bioRxiv - Developmental Biology 2022Quote: ... the cells were washed three times in 0.1 % Triton X-100/PBS (for nuclei staining 1 μg/mL DAPI (Tocris) was added to the first wash ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were seeded onto Matrigel-coated plates at a density of 15,800 cells/cm2 in E8 medium containing 10 µM Y27632 (Tocris). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... Matured iMGLs at density of 15 000 cells/well on 96-well plates were treated with 0.3-50 µM Yoda1 (Tocris) or equal volume of DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 1 uM of the microtubule-stabilizing agent SAHA (TOCRIS bioscience Cat. No. 149647-78-9) for 30 minutes before activation or experimental procedures ...
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bioRxiv - Cell Biology 2023Quote: For inhibition of P2X4R cells were pre-incubated for 30 min with 10 μM of 5-BDBD (Tocris Bioscience). Blockage of αvβ3/αvβ5 integrins was achieved by the pre-incubation with 10 μM of cilengitide for 2 hours (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... 3- (4,5-dimmethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were used to estimate cell number following the manufacturer’s description (Tocris, # 5224), and absorbance was measured at 550 nm in an EnsightTM 96-well plate reader (Perkin Elmer).
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated in Anteriorization media-1 (100 ng/ml Noggin (R&D/Biotechne) and 10 μM SB431542 (Tocris)) for 24 hours ...
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bioRxiv - Genetics 2023Quote: ... cells underwent various drug experiments: for cAMP analogs (8Br-cAMP, db-cAMP, and 6Bnz-cAMP, Tocris Bioscience, Bristol, UK), they were incubated for 48 hours with the appropriate concentrations (50 and 500µM for 8Br-cAMP and db-cAMP ...
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bioRxiv - Neuroscience 2024Quote: ... mEPSC recordings were obtained from cells voltage-clamped at –70 mV in ACSF containing 0.5 µM tetrodotoxin (TTX, Tocris) and 50 µM picrotoxin (PTX ...
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bioRxiv - Neuroscience 2024Quote: Myelin degeneration was obtained by treating myelinated Schwann cell–DRG neuronal co-cultures with 25 μM AT–56 (Tocris) in myelinating medium for additional 7 days ...
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bioRxiv - Developmental Biology 2021Quote: ... coated 12-well plates at the density of 6×105 cells/well in mTeSR1 medium supplemented with 10 μM ROCK inhibitor (Y-27632, Tocris). The medium was changed daily ...
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bioRxiv - Neuroscience 2019Quote: ... The chemical inhibition of CTL6 cells was conducted by a global perfusion of clozapine-n-oxide (CNO, 5uM, Cat# 4936, Tocris), the chemical actuator of the chemogenetic probe ...
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bioRxiv - Neuroscience 2020Quote: ... or by either bath application or using a custom-made perfusion system (for imaging cells on 12-mm coverslips): 2-AG (Tocris), AEA (Cayman) ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs WT and GPS2-KO cells were treated in the presence or absence of homoharringtonine (HHT) (5 μM, Tocris Bioscience) for 10 min to prevent new translation initiation ...
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bioRxiv - Cell Biology 2021Quote: ... flow cytometer, and the fractions of stained cells were quantified using FloJo 10.6, with Staurosporine (4 h, 5 μM) (Tocris, 1285) used as a positive control for cell death and to help establish gating of the sorted cells.
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bioRxiv - Developmental Biology 2022Quote: ... iPSCs were dissociated into single cells and plated at a density of 50,000 cells/well in a vitronectin-coated 24-well plate with 10µM of the ROCK inhibitor Y-27632 (Tocris Bioscience). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were either untreated (GFP-Apex and S1R-Apex) or treated (S1R-Apex) with 25μM Haloperidol (Tocris, Minneapolis, MN) or 20μM (+)-PTZ (Millipore-Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... cells were rinsed with ice cold PBS and then collected and suspended by scraping in PBS containing 50nM JZL195 (Tocris), a dual inhibitor of FAAH and MAGL (Long et al. ...
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bioRxiv - Cell Biology 2020Quote: ... definitive endoderm cells were generated from hESCs (∼90% confluence) by the combination of 1-2µM CHIR-99021 (Tocris, 4423-10MG) [9] and 100ng/ml ActivinA (R&D Systems ...
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bioRxiv - Developmental Biology 2020Quote: ... day 3 NMP-like cultures were treated with Accutase and replated at a density of 45,000 cells/cm2 on Geltrex-coated plates in N2B27 containing FGF2 (40 ng/ml, R&D) and CHIR99021 (8 µM, Tocris) for two days ...
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bioRxiv - Cell Biology 2021Quote: ... and seeded at a seeding density of 10000-25000 cells/cm2 in mTeSR supplemented with 2 µM ROCK inhibitor Y27632 (Tocris) onto laminin-521 coated plates ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 dissociated iPS cells resuspended in mTeSR1 media containing 10 µM Y-27362 dihydrochloride (ROCKi; Tocris Bioscience, cat. no. 1254) and 0.05% poly-vinyl alcohol (PVA ...
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bioRxiv - Genetics 2019Quote: ... cells were treated with phorbol 12-myristate 13-acetate (PMA; 100nM in DMSO) or DMSO or PKC antagonist (GF10930X; Tocris) for 24 hours following which cells were lysed for dual luciferase analysis as per manufacturer’s instructions (Promega UK).
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bioRxiv - Developmental Biology 2020Quote: Embryos were incubated at 28°C from the one cell stage to 75% epiboly (8 hpf) in E3 medium containing 5 μM of the Yap1 antagonist verteporfin (5305, Tocris) dissolved in DMSO or the same volume of DMSO as control.
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bioRxiv - Cancer Biology 2021Quote: ... and single-cell suspensions were re-plated in mTeSR1 supplemented with 2 µM ROCK inhibitor (Y-27632 Dihydrochloride Tocris #1254). Transgenic WT29 iPSCs were generated by transfecting PiggyBac expression vector and pBase (Villegas et al ...
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bioRxiv - Neuroscience 2022Quote: ... Then cells were hyperpolarized to -80 mV to isolate AMPA currents which were then were blocked by NBQX (20uM; Tocris) and finally the cells got depolarized to +40 mV to measure NMDA-mediated currents which were blocked by the selective NMDA antagonist AP-V (50 uM ...
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bioRxiv - Cell Biology 2022Quote: ... the actin depolymerizer latrunculin B (10 μM; for 30 min only to preserve cell viability; Tocris Bioscience; Thermo Fisher Scientific), or the YAP inhibitor verteporfin (0.5 μM ...
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bioRxiv - Neuroscience 2022Quote: ... All iPSC lines were first seeded concurrently on GeltrexTM (Thermo)-coated plates at 105,000 cells/cm2 (1,000,000 cells in a well of a six-well plate) in mTeSRTM medium supplemented with 10 μM Y-27632 (Tocris). The Y-27632 compound was removed from the medium the next day and subsequently the medium was replaced daily ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were continuously perfused with this saline solution during whole-cell recording with the addition of 20 μM NBQX and 20 μM (R)-CPP (Tocris) to isolate inhibitory synaptic activity ...
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bioRxiv - Cancer Biology 2023Quote: ... we washed cells once with PBS and then added various concentrations of drugs (tamoxifen, fulvenstrant, or 18albociclib; all purchased from Tocris) in phenol red free DMEM (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in an ad hoc defined generic medium (Supplementary Table 2) supplemented with Rho-kinase inhibitor (ROCKi; Tocris) and Primocin ...
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bioRxiv - Biochemistry 2022Quote: ... hiPSCs were patterned for 21 days with a growth factor cocktail to promote differentiation toward ventral midbrain neuronal progenitor cells for 11 days (10 mM SB431542, Tocris; 100 nM LDN193189 ...