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Citations for Polysciences :
351 - 400 of 616 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Each expression vector was transiently transfected into Expi293 cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Biochemistry 2021Quote: ... The expression construct was transiently transfected in HEK 293T cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... The expression construct was transiently transfected in HEK 293T cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 1.25 μg/well of the reporter plasmids by polyethyleneimine reagent (Polysciences, #23966) and differentiated in DMEM/10% FBS with 1µM rosiglitazone for two days ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was transfected into HEK293 cells using PEI MAX 40000 (Polysciences, Inc., Warrington, PA, USA). The cultures were grown in FreeStyle 293 serum-free media (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... The cells were trypsinized and fixed freshly prepared mixture of 1% glutaraldehyde (Polysciences Inc, Warrington, PA) and 1% osmium tetroxide (Polysciences Inc. ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated at 37°C for 3 days and later fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... cultures of FreeStyle 293 (Thermo) cells were transiently transfected with the constructs using PEI MAX (Polysciences) at a 1:4 (w/w ...
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bioRxiv - Microbiology 2023Quote: ... At least 1×106 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently incubated to 4 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... SV589 cells grown on glass coverslips were transfected with pEGFP-GM130 FL using PEI Max (Polysciences) and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with AAV plasmid and helper plasmid using PEI Max 40000 (PolySciences, USA). Twenty-four hours later ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was transfected into 293T cells using PEI (Polysciences, high potency linear, MW = 40 000) transfection reagent ...
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bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...
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bioRxiv - Systems Biology 2023Quote: ... cells were transfected with 1 µg of the pTwist_CMV plasmid using 3 µg of PEI (Polysciences) as a transfection reagent or with with 1 µg of the pDEST_GFP plasmid (Control) ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transfected into HeLa Kyoto and HEK293 cells using linear polyethylenimine (PEI, MW 25,000; Polysciences). One day prior to transfection ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (ATCC, Manassas, USA) were transfected with pNL-EGFP/pVSV using polyethylenimine (PEI; Polysciences, Germany) (PEI/DNA 6/1 ratio ...
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bioRxiv - Immunology 2023Quote: Raw264.7 cells in a confluent 15cm dish were incubated with ∼108 magnetic beads (1μm size, Polysciences) for 4h ...
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bioRxiv - Cell Biology 2023Quote: Immunogold electron microscopy was performed on thin sections of cells embedded in Lowicryl K4M (Polysciences Inc.). HAP1 WT or mini-NEAT1 cells fixed in situ for 1 h at 4°C with 4% formaldehyde in 0.1 M Sörensen phosphate buffer pH 7.3 were scraped off and centrifuged ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in cold 2.5% glutaraldehyde (25% glutaraldehyde stock EM grade, Polysciences, 111-30-8) in 0.01 M PBS (Fisher) ...
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bioRxiv - Biophysics 2023Quote: ... HEK293T cells were transfected with GPΔmuc and GPΔmucHRV3C plasmid using polyethyleneimine (PEI MAX, Polysciences, Warrington, PA) at a mass ratio of 1:3 PEI MAX:DNA ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: Adeno-associated viruses (AAVs) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... cells were harvested by centrifugation and fixed by the addition 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
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bioRxiv - Cell Biology 2024Quote: Plasmid transfection of 293T and HeLa cells was performed using polyethyleneimine (Polysciences Inc., Warrington, PA, USA) and HilyMax (DOJINDO Laboratories ...
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bioRxiv - Cell Biology 2020Quote: RPE1 cells were transfected with PX458 vector with specific BBS sgRNA using polyethylenimine (Polysciences, Inc., 23966-2). After 48 h ...
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bioRxiv - Cell Biology 2020Quote: ... The Ecad and Dsc3 plasmids were transiently transfected into HEK 293T cells using Polyethylenimine (PEI) (Polysciences, Inc.). Four days post transfection ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells were plated in two 10 cm2 dishes and transfected using PEI MAX (Polysciences, PA USA) with vRNP expression plasmids (3 µg/mL each of pCAGGS/PB2-FLAG ...
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bioRxiv - Neuroscience 2021Quote: ... The cells in each well were transfected with 1.6 μg PEI (Polyethylenimine, Linear, MW 25000, Polysciences, U.S.A.) and 400 ng of pMU2-Camk2b plasmids ...
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bioRxiv - Immunology 2021Quote: Pseudotyped reporter virus was produced by transfection of 293T cells with four plasmids complexed with polyethyleneimine (Polysciences): dual-reporter vector described above ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293T cells were transiently transfected with pLVX-IRES-Puro-WDR5 plasmid by PEI (Polysciences, 23966-2) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and after 4 h the cells were transfected using 60 μg polyethyleimine (PEI, Polysciences, Warrington, PA, USA). Plasmid DNA in an amount of 1.5 μg was diluted in buffer containing 0.15 M NaCl and 20 mM HEPES (pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Lentivirus was produced by transient transfection of HEK293T cells (ATCC) using linear 25 kDa polyethyleneimine (PEI; Polysciences). Briefly ...
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bioRxiv - Immunology 2021Quote: rAAV-DJ were produced in HEK293T cells (ATCC) by triple transient transfection using polyethylenimine (PEI, Polysciences Inc). For each vector ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed in 3% paraformaldehyde and were stained for 1 hour with 1% Uvitex (Polysciences), which specifically binds to the chitin of the fungal cell wall ...
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bioRxiv - Microbiology 2020Quote: ... triple plasmid transfection of 293 adherent cells was performed with a polyethylenimine (PEI)-MAX (Polysciences, MW, 40,000) to DNA ratio of 3:1 (a mass-per-mass ratio) ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was added at a final concentration of 0.675 μg/mio cells and polyethylenimine (Polysciences, #23966-1) was added to a final concentration of 0.01 mg/mL ...
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bioRxiv - Biophysics 2020Quote: ... plasmids encoding DISP1-A-NNN-SBP were transfected into the HEK293F cells using linear polyethylenimines (PEIs, Polysciences). Both DISP1-A and DISP1-A-NNN were purified essentially as previously described[17] ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were incubated using 1 mg/mL of Polyethylenimine (PEI, Linear, MW 25000, Polysciences, PA, USA) at a ratio of 1:6 (DNA:PEI) ...
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bioRxiv - Neuroscience 2021Quote: ... COS-7 cells were transiently transfected with appropriate cDNA using PEI (Polyethylenimine, linear, MW 25000; Polysciences, Inc).
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bioRxiv - Biochemistry 2020Quote: ... HDX-MS and MALS analysis were expressed in HEK293-GnT1 suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... 5*10^6 293FT cells were transfected in 10 cm dishes using 4 μg polyethylenimine (PEI, Polysciences) per μg plasmid DNA with lentiCRISPR_V2 (Addgene 52961) ...
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bioRxiv - Molecular Biology 2019Quote: ... 35µl cell suspension was applied to the wells of Teflon coated Immunofluorescence slides (Polysciences Inc, No. 18357) pre-coated with Poly-lysine ...
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bioRxiv - Microbiology 2021Quote: ... The re-circularized genome was transfected into 293TT cells with PEI max transfection reagent (Polysciences, 24765-1). Progeny viral particles were purified on a linear cesium chloride gradient and titered as described previously (27 ...
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bioRxiv - Biophysics 2021Quote: ... at a density of 5-7 million cells and transfected the next day with PEI (Polysciences, Germany) and 16:4:4:8 μg DNA encoding hASIC1a TAG variants ...
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bioRxiv - Biochemistry 2021Quote: ... Transient transfections were performed 24 h prior to cell lysis using polyethylenimine PEIMax (0.1% w/v) (Polysciences)[39] ...
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bioRxiv - Cell Biology 2020Quote: ... and cell pellets resuspended in 9.37 ml PBS plus 0.63 ml 16% formaldehyde-EM grade (Polysciences, Inc) and incubated at room temperature for 10 min ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated at 37°C for 3 days and then fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Immunology 2020Quote: ... at 10,000 cells in 100μl PBS and human serum opsonised Fluoresbrite™ Yellow Green Microspheres 1μm (Polysciences) were added at a concentration of 10:1 for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T or GP2-293 cells were used for lentivirus or retrovirus production using polyethyleneimine (PEI 25000, Polysciences). Supernatants containing lentiviral and/or retroviral particles were applied to RPE1-Tet3G-derived host cells for 24h ...
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bioRxiv - Cell Biology 2021Quote: ... 20µl of cells were spotted onto each well of a Teflon coated 10 well slide (Polysciences, 18357) that had been pre-treated with 10ng/µl poly-L-lysine ...