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Citations for Polysciences :
301 - 350 of 616 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated for the binding with Concavalin A magnetic beads (Polysciences) by rotating for 10 min at RT ...
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bioRxiv - Genetics 2023Quote: ... Briefly, HEK293T17 (ATCC CRL-11268, Manassas, VA, USA) cells were transfected using polyethylenimine (Polysciences) with the helper plasmid pxx-680 (a gift from R ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells have been transfected using a home-made solution of PEI (MW 25000, Polysciences, stock concentration 0,324 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... the plasmid and helper plasmid were transfected into HEK293T cells using polyethylenimine (# 24765, Polysciences). The virus was harvested after 72 h and purified from benzonase-treated cell lysates via an iodixanol density gradient (OptiPrep ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells have been transfected using a home-made solution of PEI (MW 25000, Polysciences, stock concentration 0,324 mg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... and delivered to HEK293T cells by transient transfection using linear 25 kDa polyethyleneimine (Polysciences). About 24 h post-transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with the corresponding plasmids using polyethylenimine (1 μg/μL [pH 7]; Polysciences) in a 1:3 ratio ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells growing at 50-70 % confluence were transiently transfected using linear polyethylenimine (M.W. 25000, Polysciences) as described (Chuykin et al. ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV-RC and pAAV-Helper plasmids into 293T cells using PEI MAX (Polysciences; Pennsylvania, USA). 72 h after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen sections and plated cells were mounted following antibody staining using AquaPolymount (Polysciences, Heidelberg, Germany) and imaged using a Zeiss Apotome ImagerZ2 (Zeiss ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T and HeLa cells were transiently transfected with plasmid DNA using polyethylenimine (PEI, Polysciences) at a final concentration of 5 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... and pMD2.G were co-transfected into 293T cells using polyethyleneimine (PEI, Polysciences 23966-1). Virus-containing supernatant media were harvested 48 hours post transfection and passed through 0.45 µm filters ...
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bioRxiv - Biophysics 2021Quote: ... RPE1 cells were transfected with 90 μL of Polyethylenimine (PEI MAX #24765 Polysciences, Warrington, PA) and 15 μg of the pX335-gRNA and pUC19-homology arms-iRFP plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... Briefly sub-confluent HEK 293 cells were transfected with branched or linear PEI (Polysciences, Inc.) 18 to 24 hrs after transfection the cells were lysed and incubated with CycB1 antibody overnight at 4°C and Protein G Sepharose beads (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells stably transduced with HSPB8 were transiently transfected using PEI MAX (24765-1, PolySciences Europe ...
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bioRxiv - Molecular Biology 2020Quote: ... YFP-TOP2B was transfected into HEK293F cells in Hyclone TransFx media (Cytiva) using PEI (Polysciences) and purified using the YFP-tag system (Schellenberg et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were washed with PBS and then post-fixed in 1.5% osmium tetroxide (Polysciences) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Cell Biology 2019Quote: ... into HEK 293T cells using 1mg/mL of PEI Max 40000 (Polysciences, cat. 24765-1). After 48 hours of transfection ...
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bioRxiv - Microbiology 2019Quote: ... and pVSV-G into 293T cells via polyethylenimine HCl MAX (PEI, Polysciences, Cat No: 24765) diluted in Opti-MEM (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... Transient transfections were performed 40-48 hr prior to cell lysis using polyethylenimine PEI (Polysciences) at around 60-70% confluence ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were incubated with 0.1 μg/mL DAPI and mounted in Poly mount (Polysciences; 18606).
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bioRxiv - Microbiology 2020Quote: ... Cells were then cross-linked with 1% formaldehyde (Ultra Pure EM grade, Polysciences, Warrington, PA), for 10 min at 26°C followed by another 5 min incubation after adding glycine to a final concentration of 125 mM to stop the cross-linking reaction ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences). For intracellular transcription factor staining ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences).
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bioRxiv - Bioengineering 2020Quote: ... The cells were transiently transfected with ACE2 plasmids and polyethylenimine (PEI) (Polysciences, Cat.24765-1) when the cell density reached approximately 1.0×106/mL ...
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bioRxiv - Biochemistry 2022Quote: LRRK2 R1441G was transfected into HEK293T cells with Polyethylenimine HCl MAX 4000 (PEI) (Polysciences, Inc.) and purified 48hrs post transfection ...
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bioRxiv - Biochemistry 2022Quote: ... adherent HEK293T cells were transfected with the expression plasmids using linear PEI (MW 25000, Polysciences). In detail ...
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bioRxiv - Microbiology 2022Quote: ... The cells were trypsinized and fixed in 4% paraformaldehyde/0.01% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviruses for Cas9 and sgRNAs were produced in 293T cells using standard polyethylenimine (Polysciences Inc.) transfection protocols.
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences) and acquired on a BD LSR Fortessa using BD FACS Diva.
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bioRxiv - Biophysics 2023Quote: ... Then the cells were pre-fixed for 1 min in 0.5% PFA (Formaldehyde, Polysciences, Inc) in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were transfected with pCMV14-SMCT1 using PEI MAX pH 6.9 (MW 40,000; Polysciences) and cultured for two days.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were transiently transfected with 200 ng MyD88-Venus construct using PEI Max (Polysciences) according to manufacturer’s protocol and were incubated for further 24 h at 37 °C in a 5% CO2 incubator ...
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bioRxiv - Neuroscience 2024Quote: N2A-Pz1-KO cells were transfected using PEI-Max (Polysciences Inc, Cat. Nu. 24765-100) with 1500 ng ptdTomato-N1 vector ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... or pMSCV-puro-based constructs were transiently transfected in HEK293T cells using polyethylenimine (Polysciences, 24765). For lentivirus production ...
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bioRxiv - Cell Biology 2024Quote: Equal numbers of HEK293T cells were seeded Wnt7a plasmid was transfected with linear polyethylenimine (Polysciences), accordingly to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... NIH3T3 cells were transfected with 1 μg/ml plasmid with polyethyleneimine (3 μg/ml; Polysciences, 23966). Media was changed the next day and cells harvested 48 hrs later ...
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bioRxiv - Cell Biology 2021Quote: ... MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.), cultured for 16 h ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stained with benzidine in combination with the Differential Quick Stain Kit (PolySciences, Warrington, PA) (manufacturers protocol) ...
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bioRxiv - Cancer Biology 2019Quote: Retrovirus was produced in Plat-E cells by transient transfection with PEI (Polysciences; Cat#23966-1) according to standard protocols ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected with plasmids encoding ACE2 orthologs or human DPP4 using PEI 40K (Polysciences) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The fixed and stained cells were washed with PBS and mounted using Aqua-Poly/Mount (Polysciences). Confocal images of infected cells were acquired using a Leica SP8 Scanning Confocal Microscope controlled by Leica-X software ...
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bioRxiv - Biochemistry 2022Quote: ... 30,000 cells per 48 well were plated and transfected with 1 μl PEI (Polysciences, PEI 25K), 75 ng of promoter reporter plasmids (3xMBS-pGL4.10 or pGl4.10 empty vector ...
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bioRxiv - Microbiology 2022Quote: ... or the SLC35A1-targeting plasmid to generate SLC35A1KO cells using polyethylenimine (PEI; Polysciences, Warrington, PA, USA). At 24 h post-transfection ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were dehydrated with increasing amounts of ethanol and embedded in epon epoxy resin (Polysciences). Ultrathin sections of 60 nm were contrasted with uranyl acetate and lead citrate using an AC20 automatic contrasting system (Leica ...
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bioRxiv - Immunology 2020Quote: ... The vector with antigen-bearing component was transfected into FreeStyle 293F cells using polyethylenimine (Polysciences, Inc) as described previously 7 ...
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bioRxiv - Biochemistry 2020Quote: ... The expression construct was transiently transfected in HEK 293F cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... COS-7 and HEK293 cells were transfected with DNA plasmids using PEI (Polysciences, Inc., Warrington, PA). At 24 hours post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were maintained in FreeStyle 293 medium and transfected using the PEI Max reagent (Polysciences Inc.) with a 1:3 ratio (w/w ...