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Citations for Polysciences :
601 - 650 of 745 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: HELBKO HEK293T cells were transfected with plasmids encoding pcDNA5/FRT/TO-SFB-HELB variants using PEI (Polysciences, Inc). After 24 hours ...
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bioRxiv - Biophysics 2024Quote: ... Cells were transfected using a polyethylenimine (PEI) agent (10 μl of 1 mg/ml per dish hereafter; Polysciences) with pCAG plasmids encoding human A1R ...
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bioRxiv - Microbiology 2020Quote: ... infected cells then were fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Immunology 2021Quote: ... pSIREN- or pMXs-based plasmids were co-transfected together with pVSVG into Plat-E cells (kindly provided by T. Kitamura, University of Tokyo) using PEI Max (Mw 40,000, 24765-1; Polysciences). The virus-containing supernatant was harvested 2 days after transfection ...
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bioRxiv - Genomics 2020Quote: All lentiviruses were produced by co-transfecting lentiviral backbones with packaging vectors (delta8.2 and VSV-G) into 293T cells using PEI (Polysciences). The viral-containing supernatant was collected at 48- and 72-hours post-transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Combinations of equimolar quantities of constructs were co-transfected into cells using linear polyethylenimine (PEI) (Polysciences, Warrington, PA, USA). DNA (4 μg ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T cells in a 10 cm dish were transfected with 40 µL 1.0 mg/mL polyethylenimine (Polysciences, Warrington, PA) and 15 µg plasmid and incubated for 24 h ...
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bioRxiv - Microbiology 2020Quote: ... was performed by transfecting HEK293T cells in five 15 cm tissue culture plates using PEI (Polyethylenimin Linear, MW 25,000, 23966-1-A, Polysciences) transfection method (45) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were transfected with 1 μg/ml of purified plasmid DNA and 2 μg/ml of polyethylenimine (Polysciences Inc.). Cells were harvested 72 hours post transfection and frozen at −80°C until used ...
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bioRxiv - Cancer Biology 2021Quote: All lentiviruses were produced by co-transfecting lentiviral backbones with packaging vectors (delta8.2 and VSV-G) into 293T cells using PEI (Polysciences). The viral-containing supernatant was collected at 48- and 72-hours post-transfection ...
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bioRxiv - Immunology 2021Quote: ... Two vectors containing paired TCRα and β chains were transfected together into Phoenix packaging cells using PEI MAX (Polysciences). Supernatant containing retroviruses was used for infection into mouse T cell hybridoma with an NFAT-GFP reporter gene (Matsumoto et al. ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293T cells were transfected in 100 mm dishes using 8 µg plasmid DNA and 24 µl PEI (Polysciences, Inc). DNA was mixed with PEI in Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: Cells destined for immunofluorescence were washed with PBS once and fixed using 4% wt/vol paraformaldehyde (PFA, Polysciences Inc.) for 20min ...
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bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
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bioRxiv - Microbiology 2020Quote: Constructs were introduced into HEK293F cells by high-density transfection with PEI MAX 40K (Polysciences, Inc., Warrington, PA, USA) (47 ...
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bioRxiv - Immunology 2020Quote: ... some of the prepared BALF cell spots were fixed and treated with Wright-Giemsa stain (Polysciences, Inc., Warrington, PA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The pTT5-VHH72hFc1X7 plasmid was transiently expressed in CHO55E1 cells (34) using PEI-Max transfection reagent (Polysciences, Warrington, PA) and a slightly modified protocol as described previously (35) ...
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bioRxiv - Microbiology 2022Quote: Soluble PCDH1 variants cloned into pcDNA3.1 (see above) were expressed in 293F cells in shaker flasks by transient transfection with linear polyethyleneimine (Polysciences), as described previously (56) ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Immunology 2022Quote: ... expi293F cells were transfected with antibody expression plasmids at a density of 2.5 million cells per/ml using 1 mg/ml PEI-Max transfection reagent (Polysciences). Supernatants were harvested 4-5 days post-transfection and tested for their SARS-CoV-2 RBD binding potential by enzyme-linked immunosorbent assay (ELISA) ...
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bioRxiv - Neuroscience 2023Quote: AAV8 viruses were produced in HEK293FT cells grown on 5 plates (linear PEI, Polysciences Europe Cat. No. 23966-100) or 10 plates (branched PEI ...
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bioRxiv - Neuroscience 2023Quote: ... medium was reduced to half and cells were transfected with a mixture of DNA/polyethyleneimine (PEI) complexes (MW40000, PolySciences). Complexes were formed by combining 500 ng of plasmid DNA in 10 μL of serum free DMEM and 3 μL of PEI (1 mg/ml ...
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bioRxiv - Microbiology 2023Quote: HIV-1 viruses were generated by transfection of proviral DNA into HEK293T cells via polyethylenimine (PEI, Polysciences, Cat. #23966) transfection protocol ...
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bioRxiv - Microbiology 2023Quote: ... 1 million HEK293T cells were seeded into 6-well plates and transfected using polyethylenimine (PEI) (#23966-1; Polysciences, Inc.) with 1 μg of the indicated DNA constructs at a 1:5 ratio with PEI ...
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bioRxiv - Biophysics 2022Quote: ... Cells were plated to 1.5 x 105 cells per well in 12-well plates 24 hours prior to transfection with polyethylenimine PEI (Polysciences). The primary transfection mixtures contained 125 ng pFLuc-TAR-140 reporter plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transfected with shRNA constructs and packaging plasmids (psPAX2 and VSV-G) using PEI (polyethylenimine MW25,000, Polysciences). Viral supernatants were collected at 48h and 72h post-transfection and mixed ...
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bioRxiv - Cell Biology 2023Quote: ... lentiviral vector was co-transfected with pSPAX2 and pMD2.G in 293T cells using 40-kDa linearpolyethylenimine (Polysciences, 24,765). Retrovirus generation was by using PLAT-A cells (Cell Biolab) ...
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bioRxiv - Immunology 2023Quote: ... Cell pellets were infiltrated with a 1:1 mixture of propylene oxide and Polybed 812 epoxy resin (Polysciences, Inc.) for 2 hours and infiltrated overnight ...
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bioRxiv - Molecular Biology 2023Quote: HEK 293T cells were transfected with lentiviral constructs and packaging plasmids (psPAX2 and pMD2.G) using PEI transfection (Polysciences). Two days post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were counterstained with DAPI (nuclear stain) in PBS and mounted with Aqua-Poly/Mount medium (18606-5, Polysciences). Representative pictures for figures were exported from whole slide scans using the snapshot function of CaseViewer (Build 2.4.0.119028).
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bioRxiv - Biochemistry 2024Quote: ... Cells were seeded in 35-mm glass-bottom cell-culture dishes (Iwaki) and transiently transfected with the eLACCO2.1 and deLACCO1 using polyethyleneimine (Polysciences) prior to imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were transfected with indicated DNA constructs and tubulin-Gal4 (Lee and Luo, 1999) using polyethyleneimine (Polysciences, Warrington, PA) (Longo et al. ...
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bioRxiv - Biochemistry 2020Quote: ... ADP-Glo kinase assays and liposome sedimentation assays were expressed in HEK293-GnTI suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Cell Biology 2020Quote: Cells at 30-40% confluency were transfected with 2µg DNA in six well plates using polyethylenimine (PEI; #23966, Polysciences Inc.). pcDNA3.1 or pBlueScript were used as empty vector controls ...
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bioRxiv - Immunology 2022Quote: ... The S-2P and S-6P plasmids were transiently transfected into HEK293F cells using 25-kDa linear polyethyleneimine (PEI) (Polysciences) with the PEI:DNA mass ratio of 3:1 and 1 mg DNA for per liter of culture when the cell density reached 2 × 106 cells per mL ...
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bioRxiv - Molecular Biology 2021Quote: Retrovirus packaging cells were transfected with pQCXIP-hMETTL18-HA or pQCXIP-hMETTL18-Asp193Lys-Gly195Arg-Gly197Arg-HA using PEI transfection reagent (Polysciences) and cultured for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 500 ng of GFP-LRRK2 construct and 500 ng of GFP-Rab10 using polyethylenimine (PEI, Polysciences). After 36 h ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 500 ng of reporter and miRNA-vector using 4 μg polyethylenimine (PEI) (Polysciences Inc, 24765-1). Dual-luciferase assays were carried out using the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... pcDNA3.1 plasmids encoding for FLAG-CHD4 were transfected into cells using linear 25-kDa polyethylenimine (PEI, Polysciences, Warrington, PA, USA). 50 µg of the DNA mixture was first diluted in 2.7 mL of PBS and vortexed briefly ...
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bioRxiv - Immunology 2021Quote: ... Env plasmids were co-transfected in Human Embryonic Kidney 293T or GnT1-293S cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid (23 ...
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bioRxiv - Microbiology 2021Quote: ... expression medium was added to reduce cell density to 1.0 × 106 ml−1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Biochemistry 2021Quote: ... and wildtype b0,+AT (the pEZT-C-TwinStrep-mCherry-OaSLC7A9 plasmid) were transfected into HeLa cells at 1:1 molar ratio by using PEI MAX (40,000 MW Linear; Polysciences, Inc.). The ratio of DNA ...
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bioRxiv - Biochemistry 2020Quote: Plasmids encoding human EMC4 with an N- or C-terminal 3xHA tag in the pcDNA5/FRT/TO vector were transiently transfected into HEK293T cells using PEI “MAX” (Polysciences). At 3 days after transfection ...
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bioRxiv - Biochemistry 2020Quote: ... All protein encoding sequences were subcloned to pCAG vector with N-terminal FLAG-tag for expression in HEK293F cell lines transfected by polyethylenimines (PEIs, Polysciences). Approximately 1.5 mg plasmids were incubated with 3 mg PEIs in 50 mL SMM 293T-I medium (Sino Biological Inc. ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with FACS buffer and fixed in 200 µl of 2% ultrapure formaldehyde (Polysciences, Warrington, PA) diluted in FACS buffer (fixation buffer) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were seeded in 35-mm glass-bottom cell-culture dishes (Iwaki) and transiently transfected with the constructed plasmid using polyethyleneimine (Polysciences). Transfected cells were imaged using a IX83 wide-field fluorescence microscopy (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 50 to 100 million J-Lat 11.1 cells per condition were collected and crosslinked with 1% formaldehyde (Polysciences inc.) in 40 ml PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Purified cDNA was incorporated into a pcDNA3.1 vector and transfected into HEK293T cells using PEI max transfection reagent (Polysciences, Germany) as described previously (18) ...
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bioRxiv - Cell Biology 2020Quote: ... Each plasmid and packaging vectors (pCMV-VSV-G-RSV-Rev and pCAG-HIVgp) were used to transfect HEK293T cells with Polyethylenimine MAX transfection reagent (Polysciences), according to the manufacturer’s instructions ...