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Citations for Polysciences :
551 - 600 of 745 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 5*10^6 293FT cells were transfected in 10 cm dishes using 4 μg polyethylenimine (PEI, Polysciences) per μg plasmid DNA with lentiCRISPR_V2 (Addgene 52961) ...
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bioRxiv - Molecular Biology 2019Quote: ... 35µl cell suspension was applied to the wells of Teflon coated Immunofluorescence slides (Polysciences Inc, No. 18357) pre-coated with Poly-lysine ...
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bioRxiv - Microbiology 2021Quote: ... The re-circularized genome was transfected into 293TT cells with PEI max transfection reagent (Polysciences, 24765-1). Progeny viral particles were purified on a linear cesium chloride gradient and titered as described previously (27 ...
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bioRxiv - Biophysics 2021Quote: ... at a density of 5-7 million cells and transfected the next day with PEI (Polysciences, Germany) and 16:4:4:8 μg DNA encoding hASIC1a TAG variants ...
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bioRxiv - Biochemistry 2021Quote: ... Transient transfections were performed 24 h prior to cell lysis using polyethylenimine PEIMax (0.1% w/v) (Polysciences)[39] ...
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bioRxiv - Cell Biology 2020Quote: ... and cell pellets resuspended in 9.37 ml PBS plus 0.63 ml 16% formaldehyde-EM grade (Polysciences, Inc) and incubated at room temperature for 10 min ...
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bioRxiv - Immunology 2020Quote: ... at 10,000 cells in 100μl PBS and human serum opsonised Fluoresbrite™ Yellow Green Microspheres 1μm (Polysciences) were added at a concentration of 10:1 for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T or GP2-293 cells were used for lentivirus or retrovirus production using polyethyleneimine (PEI 25000, Polysciences). Supernatants containing lentiviral and/or retroviral particles were applied to RPE1-Tet3G-derived host cells for 24h ...
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bioRxiv - Cell Biology 2021Quote: ... 20µl of cells were spotted onto each well of a Teflon coated 10 well slide (Polysciences, 18357) that had been pre-treated with 10ng/µl poly-L-lysine ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
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bioRxiv - Biophysics 2023Quote: ... A ratio of 1.2 µg Plasmid DNA per 106 cells and 40 kDa linear PEI MAX (Polysciences) transfection reagent was used for protein expression ...
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bioRxiv - Immunology 2023Quote: ... Env plasmids were co-transfected in human embryonic kidney 293T cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid and pMV-Rev 0932 for Envs carrying native codons ...
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bioRxiv - Molecular Biology 2023Quote: Equal numbers of HEK293T cells were seeded and the different plasmids were transfected with linear polyethylenimine (Polysciences), accordingly to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transfected with expression and packing plasmids following standard calcium phosphate or polyethylenimine (Polysciences #23966) protocols ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transiently co-transfected with lentiviral vectors using PEI MAX reagent (Polysciences, Warrington, PA, USA). Four hours post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected with 1 μg/ml DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences). Conditioned medium containing the secreted recombinant protein was collected 4 days post transfection and filtered ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK-293T cells were co-transfected with 100 ng of corresponding plasmids using linear PEI (Polyethyleneimine, Polysciences). 24h after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids expressing Fcα–J and FcRL4 was co-transfected into the HEK293F cells by polyethylenimine (Polysciences). The cells were cultured at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... plasmids encoding HLA class I/II alleles [46] were transfected in HEK293T cells with PEI MAX (Polysciences).
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bioRxiv - Bioengineering 2024Quote: ... 150,000 cells/mL with 1 v/V% 500 nm fluorescent beads (Floresbrite YG carboxylate beads, Polysciences, Inc.) were encapsulated within each hydrogel on a Nunc glass bottom dish (Nunc Glass ...
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bioRxiv - Microbiology 2024Quote: ... expression medium was added to reduce the cell density to 1.0 × 106/ml for polyethyleneimine (PEI; Polysciences) transfection ...
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bioRxiv - Microbiology 2024Quote: HIV-1 Gag-iFRET plasmid DNA was transfected into HEK293T cells using a PEI transfection method (Polysciences) and into Jurkat cells using Lipofectamine LTX transfection reagent (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Cells transfected with 1 µg of plasmids containing a gene of interest (Table 4) using polyethylenimine (PEI, Polysciences). 4 hours post transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were transiently transfected with ADAMTS6 WT or ADAMTS6 EA expression plasmids using PEI MAX (24765; Polysciences) and were co-cultured with Fbn1-/- mouse embryo fibroblasts (MEFs ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant AAV vectors were produced by transfecting HEK293 cells at ~75% confluence with polyethylenimine (PEI Max; Polysciences, 24765) using a triple plasmid transfection protocol with the AAV Rep-Cap plasmid ...
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bioRxiv - Cell Biology 2022Quote: ... 20µl of cell suspension was spotted onto each well of a Teflon coated 10-well slide (Polysciences, 18357) that had been pre-treated with 10 ng/µl poly-L-lysine ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected with 2 µg total DNA and 7.35 µl polyethylenimine HCl MAX transfection reagent (Polysciences, Inc). Wells co-transfected with mClover3-TDPΔNLS and HSP104 variants received 1 µg of each plasmid ...
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bioRxiv - Biophysics 2021Quote: ... cells were transfected using Env-deficient HIV-1 backbone plasmid pSG3ΔEnv (NIH ARRRP) and 25 kDa PEI (Polysciences) as a transfection reagent ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with 67.5 µg of polyethylenimine (PEI) Max 40,000 (Polysciences, Inc, Cat. No. 24765-1) in complex with 3.15 µg of plasmid encoding a spike variant ...
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bioRxiv - Microbiology 2021Quote: HEK 293T or HeLa cells in 10-cm dishes were infected at 5 p.f.u./cell for 8 h or transfected overnight with the specified epitope-tagged plasmids using polyethylenimine (PEI, Polysciences, 2 μl of 1 mg/ml stock per μg of plasmid DNA) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1 μg/mL of each expression vector using 2 μg/mL Polyethyleneimine “MAX” (PolySciences) (Hirayama et al. ...
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bioRxiv - Microbiology 2021Quote: ... into 70% confluent monolayer of 293T cells in a 10 cm dish using polyethylenimine (Polysciences Inc, Warrington, PA.) at 4X total transfected DNA in 800 μl 150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with PBS and incubated with cold 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) in PBS for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were transiently transfected with 2mg expression plasmid per 1 liter using the Polyethylenimine (PEI) MAX (Polysciences) at a density of 1.0 x 106 cells/ml in fresh Union 293 medium ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected at 80% confluency in whole medium employing the transfection reagent polyethyleneimine (Polysciences Europe GmbH, Germany). The cells were lysed using 50 mM Tris-HCl (pH 7.3) ...
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Sequence and structural variations determining the recruitment of WNK kinases to the KLHL3 E3 ligasebioRxiv - Molecular Biology 2020Quote: ... Cells were split into 150 mm dishes and transfected with 14 μg Flag-WNK3 construct using polyethylenimine (Polysciences). Some 36 hours post-transfection ...
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bioRxiv - Immunology 2020Quote: ... The two plasmids expressing IgA1-Fc and J-chain were co-transfected into the cells using polyethylenimine (Polysciences). Four days after transfection ...
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bioRxiv - Immunology 2022Quote: ... The S2P or S6P pCMV plasmids were used to transiently transfect HEK293F cells with polyethylenimine (PEI) (Polysciences, #24765). The recombinant S6P was affinity purified from the cell supernatant by using StrepTactin resin (IBA) ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were then washed two more times with PBS before mounting in Aqua/Polymount medium (Polysciences, Inc.) on a glass slide.
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bioRxiv - Developmental Biology 2019Quote: ... and triple transfected into 10 15-cm plates of HEK293T cells using PEI transfection reagent (Polysciences, 23966- 2). 60 h after transfection ...
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bioRxiv - Genomics 2021Quote: ... was inserted lentivirally: Cas9-blastR and 3rd generation lentiviral components were transfected into HEK293T cells using PEI (Polysciences). Supernatant was harvested ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed three times with DPBS before embedding with Aqua-Poly/Mount (#18606-20, Polysciences Inc., USA) and a cover glass ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were transfected using a polyethylenimine (PEI) reagent (2.5 µl of 1 mg/ml per well hereafter; Polysciences) with these UTS2R plasmids (100 ng per well hereafter) ...
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bioRxiv - Molecular Biology 2022Quote: ... 293T media was changed and the cells were transfected using 1mg/ml of polyethylenimine (PEI) (Polysciences, 23966-2).
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bioRxiv - Molecular Biology 2022Quote: ... each 6 cm dish of cells was transfected with 2-12 μg of poly(ethylenimine) (Polysciences, Eppelheim, Germany) and 1-6 μg of plasmid DNA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with 67.5 μg of polyethyleneimine (PEI) Max 40,000 (Polysciences, Inc, Cat. No. 24765-1) in complex with 3.15 μg of plasmid encoding a spike variant ...
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bioRxiv - Biochemistry 2023Quote: HEK cells were transiently transfected at 80% confluency with CibN and CryC plasmids using polyethylenimine (PEI) (Polysciences, Inc.). Plasmids and PEI were diluted in Opti-MEM (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with 5 µg plasmid DNA and 15 µg Polyethylenimine (PEI 25K™, Polysciences, 23966-100), both previously prepared in OptiMEM (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were transfected with 121.6 μL of 1 mg/mL of linear Polyethylenimine (PEI; MW 25,000; Polysciences) and 30.4 μg of plasmid DNA diluted in Opti-MEM (Gibco) ...