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Citations for Polysciences :
401 - 450 of 534 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 3:2:1 using 1 mg/ml polyethylenimine (Cat#23966-1, Polysciences, Inc.). Retrovirus was harvested from the medium 48 h post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... and 2 μg plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 1 μg ml-1 DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences), pre-incubated for 15 min in Optimem medium (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected with 1 μg/ml DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences). Conditioned medium containing the secreted recombinant protein was collected 4 days post transfection and filtered ...
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bioRxiv - Bioengineering 2023Quote: ... Transient transfection mixtures were created by mixing 2 µg of plasmid mixture with polyethyleneimine (PEI-MAX, Polysciences) at 4 μg of polyethyleneimine per microgram of DNA ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mg of plasmids were pre-incubated with 2 mg of 40-kDa linear polyethylenimine (PEI) (Polysciences) in 25 ml of Opti-MEM™ (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... following by curing the resin overnight at 4°C in a plastic embedding mold (Cat no.-15899-50, Polysciences Europe GmbH, Germany). Polymerized sample blocks were further hardened by overnight baking at 70°C.
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bioRxiv - Cell Biology 2021Quote: Samples were crosslinked for 10 min at RT and 20 min at 4°C with 0.8% formaldehyde (methanol free, ultrapure EM grade, Polysciences, Inc; Warrington PA). Reaction was stop at RT for 10 min with the addition of Glycine to a final concentration of 0.125 M ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Plant Biology 2020Quote: ... Dehydration was performed in an acetone series, followed by treatment with propylene oxide (SERVA, Heidelberg, Germany) at 4°C and embedding in Spurr’s resin (PolySciences, Niles, IL, USA). The embedded specimens were sectioned with a Reichert-Jung Ultracut E (Reichert-Jung Optical Company ...
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bioRxiv - Cell Biology 2024Quote: Midguts were dissected in 1X PBS (Oxoid, BR0014G) and fixed for 20 minutes at room temperature in fresh 4% methanol-free paraformaldehyde (Polysciences, 18814-10) diluted in PBS ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed with fresh 3% paraformaldehyde at 4°C for 10 mins and smeared on poly-L-lysine pre-coated slides (Polysciences, Inc., USA). Cells were washed once with 1× PBS and incubated with 100 times dilution of BODIPY® FL vancomycin (Van-FL ...
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bioRxiv - Cell Biology 2022Quote: T24 cells were fixed in 2% glutaraldehyde and 2% paraformaldehyde at 4°C overnight and then postfixed in 2% osmium tetraoxide for 1.5 h at 4°C followed by dehydration with ethanol and embedding in Spurr resin (Polysciences Inc., Warrington, PA). Ultrathin sections of the samples were prepared using Ultramicrotome (Leica EM UC7 ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed samples were gradually dehydrated in an increasing series of ethanol concentrations and were embedded in a methacrylate resin (JB-4 kit, Polysciences, Warrington, PA) containing eosin and acridin orange as contrasts agents as previously described (Mohun and Weninger ...
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bioRxiv - Microbiology 2020Quote: ... Infections were performed by spinoculation for 2 hours at 1400 x g with VSVg pseudotyped HIV-1 at MOI of 5 in the presence of 1 μg/ml PEI (Polysciences) and 16 μg/ml Polybrene (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... transient transfections of 5 µg of pcDNA3.1-based vectors were performed using 25 µg of PEI MAX (Polysciences, Warrington, PA). After an additional incubation for 24 h ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were co-transfected in a 2:1 ration with the pNL4-3-inGluc vector and the spike plasmid of interest using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) to produce pseudotyped lentiviral particles ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 8 µg of plasmid DNAs (pCMV-Thy1.1-F2A-dsGFP/muSOX/SOX/vhs/BGLF5) using 48 μl Transporter 5 (Polysciences #26008) and 1ml Opti-MemI (Gibco #31985070) ...
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bioRxiv - Molecular Biology 2023Quote: ... freshly seeded 293T cells grown in DMEM supplemented with 10% FBS + 50 µg/mL gentamycin at 37°C with 5% CO2 (hereafter, supplemented DMEM) were transfected using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Microbiology 2024Quote: ... freshly seeded 293T cells were grown in DMEM supplemented with 10% fetal bovine serum (FBS) and 50 µg/mL gentamicin at 37°C with 5% CO2 and transfected using polyethylenimine (Polysciences) [34] ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 48 hours prior to fixation with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected with 2 µg total DNA and 7.35 µl polyethylenimine HCl MAX transfection reagent (Polysciences, Inc). Wells co-transfected with mClover3-TDPΔNLS and HSP104 variants received 1 µg of each plasmid ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1 μg/mL of each expression vector using 2 μg/mL Polyethyleneimine “MAX” (PolySciences) (Hirayama et al. ...
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bioRxiv - Microbiology 2021Quote: ... 1 cm catheter with biofilm formed on inside surface was fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
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bioRxiv - Microbiology 2021Quote: ... spores were harvested in KK2 buffer and fixed with 2% paraformaldehyde with 2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM cacodylate buffer (pH 7.2 ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Live animals were immobilized with 0.01% tetramisole and 0.1% tricaine in M9 buffer and mounted on 2% agarose pads or with 0.05 μm Polybead microspheres (Polysciences) diluted 1:2 in water and mounted on 5% agarose pads ...
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bioRxiv - Molecular Biology 2022Quote: ... each 6 cm dish of cells was transfected with 2-12 μg of poly(ethylenimine) (Polysciences, Eppelheim, Germany) and 1-6 μg of plasmid DNA ...
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bioRxiv - Neuroscience 2023Quote: ... and heparin (20U/ml) for 2 min at 35°C followed by 0.15M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences), 2% formaldehyde (Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and heparin (20 U/ml) for 2 min at 35°C followed by 0.15 M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences), 2% paraformaldehyde (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 4µg pMDG.2 plasmid and 4µg psPAX plasmid by using the transfection reagent Polyethylenimine (PEI) (Polysciences #23966-100) at a PEI:DNA ratio of 4:1 ...
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bioRxiv - Molecular Biology 2022Quote: ... 293T media was changed and the cells were transfected using 1mg/ml of polyethylenimine (PEI) (Polysciences, 23966-2).
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bioRxiv - Immunology 2022Quote: ... HEK293E cells were transfected at a density of 106 cells/mL using 0.5 mg/mL of pTT3-RBD-HISAVI DNA and 2 mg/mL filter sterilized pH 7.0 Polyethylenimine (PEI) MAX (40,000 MW, Polysciences). Six days later ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by 2 minutes incubation of the slices in 0.15M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences). rinsed 5 × 2 min in chilled buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The dyes injected included fluorescent and other tracers: Fast Blue (FB, case AT, 3% dilution, 2 µl; Polysciences), Diamidino Yellow (DY ...
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bioRxiv - Immunology 2024Quote: ... cells in each well were transfected with a total of 1 μg DNA (Env-expression plasmid and pCMV-rev expression helper plasmid in 4:1 ratio) mixed with 3 μg 25 kDa linear PEI (Polysciences Inc., Warrington, USA) in 200 μl 150 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... The surface of the substrate was plasma treated at 500 W for 5 min and coated with an aqueous solution of 10% (wt/vol) Poly(acrylic acid) (MW 50000, Polysciences, USA) at 2000 rpm for 15 s using a spin-coater (WS-650-23 ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope.
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected at a density of 2.5 × 106 / ml with a DNA concentration of 3 μg/ml and polyethyleneimine (Linear PEI 25 kDa molecular weight, PolySciences Inc) at 9 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... was injected into the gastrocnemius muscles and the tracer Fast Blue tracer (Polysciences cat.no. 17740-5 at 1.5% in 3μL PBS) was injected into the soleus muscles ...
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bioRxiv - Synthetic Biology 2023Quote: ... they were transfected by mixing roughly 600 ng of plasmid mixture per well with 1.6 μL Transporter 5 Transfection Reagent (Polysciences, Warrington, PA) in 60 μL 150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... vectors were transfected to 50-80% confluent HEK293T cells in 35-mm dish using PEI-MAX (25 mM, 5 μL/dish; Polysciences, Inc.). Twenty-four hours after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... Control and 5-FU-miR-129 groups were treated with either 75 μg of PEI-MAX (control) (Polysciences, Warrington, PA, USA) or 75 μg PEI-MAX + 75 μg 5-FU-miR-129 (5-FU-miR-129) ...
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bioRxiv - Cell Biology 2022Quote: ... Whole larva were then mounted between a 2% M9 agarose pad and coverslip in M9 containing 0.1μm polystyrene beads (Polysciences, Inc.) and 10 mM Levamisole to induce worm paralysis (Reich et al. ...
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bioRxiv - Microbiology 2021Quote: Prior to flow cytometry analysis 2 μl of fluorescent microspheres (1×10−3 of the stock Fluoresbrite® YG Microspheres 0.75μm, Polysciences) was added and the volume was adjusted to 500 μl by adding FACSFlow solution (10 mM phosphate-buffered saline ...