Labshake search
Citations for Polysciences :
351 - 400 of 534 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... 9 µg transfer vector and 2 µg pVSV-G using 60 µl linear PEI (Polysciences). Medium was exchanged 6-8 hours post-transfection and harvested after another 48 hours ...
-
bioRxiv - Physiology 2023Quote: ... Sections were picked up on 1 x 2-mm formvar-coated copper slot grids (Polysciences) and further stained with uranyl acetate followed by lead citrate ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... with one or more constructs using polyethylenimine (PEI, Polysciences, Inc.; Warrington, PA, USA, 23966-2).
-
bioRxiv - Biophysics 2020Quote: ... Beaded cantilevers were generated by first drying 6.0 μm carboxylate beads onto a coverslip (17141-5, Polysciences, Inc); a small amount of UV-curable glue (NOA81 ...
-
bioRxiv - Molecular Biology 2021Quote: ... was transfected into each 10 cm dish of HEK293T cells together with the packaging vectors psPax2 (7.5 μg) and pCMV-VSV-G (5 μg) using PEImax (67.5 μg; Polysciences) or Lipofectamine-2000 (50 μl ...
-
bioRxiv - Neuroscience 2024Quote: Serotype 8 adeno-associated viruses (AAV8) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich #408727) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5 µg of transfer plasmid were transfected with polyethylenimine (8mg/mL sterlile water, Polysciences, Warrington, PA, USA). After 60–72 h of incubation ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were transfected with 5 µg plasmid DNA and 15 µg Polyethylenimine (PEI 25K™, Polysciences, 23966-100), both previously prepared in OptiMEM (Gibco ...
-
bioRxiv - Neuroscience 2024Quote: ... young adult animals were mounted onto 1-1.5% of agar pad with 3-5 µL of latex beads (Polybeads, 0.2 µm, PolySciences) to facilitate body movement and stabilize the position of field of view22 ...
-
bioRxiv - Biochemistry 2024Quote: ... packaging plasmids at a 5:3.75:1.25 ratio to HEK293T cells using PEI transfection reagent (Polysciences Inc., 23966). Viral supernatants were collected 48 h and 72 h post-transfection and filtered using a 0.45 μm syringe filter ...
-
bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
-
bioRxiv - Developmental Biology 2021Quote: Adult guts were dissected in PBS and fixed twice during 20 min in fresh 4% paraformaldehyde diluted in PBS (18814-10, Polysciences) with a 10 min wash in PBT 0,1% (PBS + Triton X100 Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 500 ng of reporter and miRNA-vector using 4 μg polyethylenimine (PEI) (Polysciences Inc, 24765-1). Dual-luciferase assays were carried out using the Dual-Luciferase Reporter Assay System (Promega ...
-
bioRxiv - Biochemistry 2020Quote: ... HEK293T cells were co-transfected with pAS-Pyl-AF and an amber codon-containing EMC construct at a ratio of 4:1 using PEI “MAX” (Polysciences) and were grown for 2 days in the presence of 0.5 mM AbK (Iris Biotech) ...
-
bioRxiv - Cell Biology 2021Quote: HEK293T cells grown in 60 mm dish to 70-80 % density were transfected with a mixture of 4 μg plasmid DNA and 20 μg polyethylenimine (Polysciences). Cell lysates were extracted 24 hrs after transfection.
-
bioRxiv - Pathology 2021Quote: ... then 1:2 mixture of propylene oxide and Polybed 812 epoxy resin for 4 hours and infiltrated overnight in 100% resin (08792-1, Polysciences). Samples were transferred to embedding molds and polymerized at 60□ overnight ...
-
bioRxiv - Biochemistry 2021Quote: ... ~1.8 mg pCAG-ATP8B1 and ~0.3 mg pCAG-CDC50A/B were incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
-
bioRxiv - Bioengineering 2020Quote: ... The pore size of isotropic and anisotropic mineralized collagen scaffolds was analyzed following a JB-4 (Polysciences, Inc., Pennsylvania, USA) embedding procedure 30,47 ...
-
bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
-
bioRxiv - Microbiology 2023Quote: ... Monolayers were washed twice with DPBS and fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc.) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
-
bioRxiv - Neuroscience 2023Quote: ... and either fixed for 4 h in 4% PFA in PBS at 4 °C and cryopreserved in 20% sucrose in PBS overnight at 4 °C or directly cryopreserved and then frozen in OCT compound (Polysciences). Sections were prepared at 14 μm ...
-
bioRxiv - Biochemistry 2023Quote: ... 5 mL expression medium were supplemented with 750 μg β2m-HLA-A*68:02Fos and 750 μg TAPBPRJun DNA (equimolar ratio) and mixed with a 4-fold molar excess of linear PEI (Polysciences) diluted in 5 mL expression medium ...
-
bioRxiv - Biophysics 2022Quote: ... 100 μg mL-1 streptomycin and 5% CO2 atmosphere at 370 C were transiently transfected with wild type human and rat NTSR1 in pcDNA3.1 (4 μg DNA per 75% confluent 10 cm dish) via hybridization with polyethylenimine (PEI, Polysciences, Inc.). The following day ...
-
Brain-wide mapping of oligodendrocyte organization and oligodendrogenesis across the murine lifespanbioRxiv - Neuroscience 2024Quote: ... the tissue was washed 4 times with 20mM PB buffer and sections were mounted onto slides with Aqua Polymount (18606, Polysciences) and allowed to dry for 24-48 hours at 4°C prior to imaging ...
-
bioRxiv - Biophysics 2024Quote: ... diluted 1:100 in nanopure water were diluted 1:10 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in nanopure water and spin coated (WS-650 ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 mg/mL plasmid DNA was mixed with 2 mg/mL linear 25 kDa polyethyleneimine (Polysciences) and transfected in OptiPRO SFM medium (4% final volume) ...
-
bioRxiv - Microbiology 2023Quote: ... At least 1×106 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently incubated to 4 °C overnight ...
-
bioRxiv - Microbiology 2024Quote: ... cells were harvested by centrifugation and fixed by the addition 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
-
bioRxiv - Cell Biology 2023Quote: ... plasmids were generally transfected at 50-60% of confluency using PEI (polyethyleneimine, Polysciences Inc #23966-2)/ Lipofectamine LTX (Invitrogen #15338100 ...
-
bioRxiv - Molecular Biology 2024Quote: ... at a 2:1:1 ratio using 3 μg 25 kDa linear polyethylenimine (PEI) (Polysciences Inc.) per μg DNA ...
-
bioRxiv - Developmental Biology 2020Quote: ... High resolution episcopic microscopy (HREM) was performed as previously described97 using an JB-4 embedding kit (00226-1, Polysciences GMBH, Germany). μMRI and HREM data were analysed using OsiriX MD DICOM viewer version 9.0.2 (Pixmeo) ...
-
bioRxiv - Bioengineering 2020Quote: ... and 4 μg CD19-CAR-GFP transfer plasmid (Bloemberg et al. 2020) with 24 μg linear PEI (Polysciences Inc., Warrington, PA) for 6 hours ...
-
bioRxiv - Bioengineering 2020Quote: ... and 10 ng of the TetR reporter plasmid pTS1017) was added into 300 μL DMEM and mixed with 4 μg of polyethyleneimine (Polysciences Inc.). The transfection mixture was vortexed and incubated for 20 min before adding into the inner 60 wells of the cell culture plate ...
-
bioRxiv - Bioengineering 2020Quote: The pore size of mineralized collagen and mineralized collagen-amnion scaffolds was analyzed following a JB-4 (Polysciences, Inc., Warrington, PA) embedding procedure (9,25) ...
-
bioRxiv - Physiology 2021Quote: ... The 4-μm bone sections were stained with the von Kossa method and counterstained with tetrachrome (Polysciences Inc., Warrington, PA, USA). The 8-μm sections remained unstained to measure fluorochrome-based indices of bone formation.
-
bioRxiv - Cell Biology 2022Quote: ... were transiently co-transfected with 4xPylT/PylRS(Y306A, Y384F) and 4xPylT/EMC3(Amber[TAG])-GFP plasmids at a ratio of 4:1 using PEI “MAX” (Polysciences, USA). The cells were grown in the presence of 0.5 mM AbK (Iris Biotech ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells at around 70% to 80% confluency in a 24-well plate were transfected with 500 ng/well of plasmid constructs expressing firefly luciferase using 4 ng/µl of polyethyleneimine (Polysciences, inc.). A plasmid expressing Renilla luciferase was co-transfected (50 ng/well) ...
-
bioRxiv - Cell Biology 2020Quote: ... various stages of the transgenic animals were mounted on 5 % agar pads with polystyrene nanoparticles (Polysciences, 2.5 % by volume) as previously described43 and imaged at an AxioImager M.2 fluorescence microscope (Zeiss ...
-
bioRxiv - Microbiology 2022Quote: ... B16F10 cells (2,500,000 cells) were transfected with 5 μg S expression plasmid by PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... For formation of DNA:PEI complexes 1 μg DNA/mL transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
-
bioRxiv - Neuroscience 2023Quote: AAV8 viruses were produced in HEK293FT cells grown on 5 plates (linear PEI, Polysciences Europe Cat. No. 23966-100) or 10 plates (branched PEI ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were counterstained with DAPI (nuclear stain) in PBS and mounted with Aqua-Poly/Mount medium (18606-5, Polysciences). Representative pictures for figures were exported from whole slide scans using the snapshot function of CaseViewer (Build 2.4.0.119028).
-
bioRxiv - Bioengineering 2024Quote: ... nominal spring constant 0.08 N/m) were calibrated using the thermal method and one 25-μm diameter polystyrene sphere (07313-5, Polysciences) was affixed to the end of each cantilever with a small amount of UV-cured glue (AA 349 ...
-
bioRxiv - Neuroscience 2023Quote: ... Worms were mounted with cover glass on 5% agarose pads with a drop of polystyrene beads (Polysciences 00876-15) as a friction raising agent ...
-
bioRxiv - Biochemistry 2024Quote: ... and 0.5-1 μg/mL of each construct was transfected with 5 μg/mL polyethyleneimine (PEI-25K; Polysciences, 23966). 24 hr after transfection ...
-
bioRxiv - Cell Biology 2020Quote: RPE1 cells were transfected with PX458 vector with specific BBS sgRNA using polyethylenimine (Polysciences, Inc., 23966-2). After 48 h ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 uL (or 1.5 uL for target-binding experiments) of 1 mg/mL PEI (Polysciences, 24765-2) dissolved in water was added to DNA in media and vortexed for 10 seconds ...
-
bioRxiv - Biochemistry 2020Quote: ... HEK 293T cells were transiently transfected with pLVX-IRES-Puro-WDR5 plasmid by PEI (Polysciences, 23966-2) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by 10 mins incubation with 1 mL of 2 mg/mL Polyethylenimine (PEI) (Polysciences, POL23966-1). As next step ...
-
bioRxiv - Immunology 2020Quote: Peeled dermis and epidermis sheets were cut to 1 cm2 and fixed in 2% formaldehyde (Polysciences, 18814) and 30% sucrose in PBS for 24 hours at 4°C ...