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Citations for Polysciences :
351 - 400 of 796 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... Transfection of HEK293T cells was carried out using polyethylenimine (Polysciences Inc.). Following concentration ...
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bioRxiv - Microbiology 2020Quote: ... to 0.8×10e6 cells/ml were transfected with linear PEI (Polysciences) with 397ug DNA at a 1:3 DNA:PEI ratio ...
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bioRxiv - Genomics 2020Quote: ... into HEK293T cells using a 1 mg/ml PEI solution (Polysciences). Lentiviral supernatants were collected at 48 hours and 72 hours after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... and Cos-1 cells with “Max” polyethylenimine (Polysciences, Warrington, PA, USA) according to the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2021Quote: ... and HEK-293T cells were transfected using polyethylenimine (#23966-1, Polysciences), while the RPE-1 cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were bound to Concanavalin A beads (86057-3, Polysciences Inc), followed by permeabilization using a digitonin containing buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transiently transfected with plasmid DNA using polyethylenimine (PEI, Polysciences), with a 1:3 DNA:PEI ratio ...
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bioRxiv - Neuroscience 2022Quote: ... Mammalian cell transfections were carried out with 1 mM PEI (Polysciences) solution at 2:1 ratio μl reagent to μg plasmid DNA for 24-72H depending on downstream use ...
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bioRxiv - Biochemistry 2022Quote: ... cells were grown overnight and transfected using polyethyleneimine “Max” (Polysciences, Inc.) as previously described [20].
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bioRxiv - Cell Biology 2022Quote: Plasmid transfection into HEK293T cells was performed using PEI Max (Polysciences) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected using 1 mg/ml PEI MAX® (Polysciences). Media was replaced 16 h post-transfection and viral supernatants were harvested through a 0.45µm filter 48 h post transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected at 40-50% confluence using PEI (Polysciences Inc.) [47] and used 24 hours after transfection.
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bioRxiv - Microbiology 2022Quote: ... The cells were transfected by using polyethyleneimine (PEI) transfection reagent (Polysciences). Transfected cells were further incubated at constant shaking speed for 4 days before harvesting the culture supernatant ...
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bioRxiv - Cancer Biology 2019Quote: ... Chromatin from HT-144 cells was fixed with 1% formaldehyde (Polysciences) and prepared with Magna ChIP™ HiSens chromatin immunoprecipitation kit (EMD Millipore) ...
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bioRxiv - Developmental Biology 2020Quote: ... Constructs were transfected together into HEK293 cells using polyethylenimine (PEI, Polysciences) with a 24-h incubation time for protein expression ...
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bioRxiv - Physiology 2021Quote: ... Cells were transfected with Polyethylenimine-PEI Transfection Reagent (Polysciences, 23966-2) carrying the LC3-eGFP plasmid (60 ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293F cells were transfected using PEI 25K linear transfection reagent (Polysciences). Expression was performed at 37°C for 7 hours followed by a second expression phase at 30°C for 72 hours.
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bioRxiv - Biophysics 2021Quote: ... The plasmid constructs were transfected into cells using polyethyleneimine (Polysciences Inc.) (Baldi et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Cultured cell lines were transfected with plasmids using PEI MAX (Polysciences) complexes with a DNA to PEI MAX ratio of 1:3 w/w as previously described49 ...
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bioRxiv - Cancer Biology 2022Quote: HEK293T cells were transfected with plasmids using polyethylenimine (PEI) (Polysciences, #24765). 4.0μg total plasmid DNA was transfected for 48 h.
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bioRxiv - Biochemistry 2022Quote: ... cells were reverse transfected using linear polyethyleneimine (25 kDa, Polysciences 23966) with LuTHy constructs and cells were subsequently incubated for 48 h ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were transiently transfected with the constructed plasmid using polyethyleneimine (Polysciences). Transfected cells were imaged using a IX83 wide-field fluorescence microscopy (Olympus ...
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bioRxiv - Cancer Biology 2022Quote: ... Transfection of packaging cells was performed using polyethyleneimine (Polysciences, 23966-2) by mixing with DNA in a 3:1 ratio ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Transfection of HEK293T cells was carried out using polyethylenimine (Polysciences Inc.). We used a second-generation packaging system to generate the lentiviruses used in this study (gift from Connie Cepko Harvard Medical School) ...
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bioRxiv - Immunology 2022Quote: ... These plasmids were transiently transfected into HEK293F cells using polyethylenimine (Polysciences) to express the corresponding proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell transfection was performed using polyethylenimine (PEI) (23866-1, Polysciences, Inc) according to manufacturer instructions.
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T cells were transfected with plasmids using polyethylenimine (PEI; Polysciences Inc.) at PEI:DNA ratio 2.5:1 ...
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bioRxiv - Cell Biology 2023Quote: ... FreeStyle 293-F cells were transiently transfected using polyethyleneimine (Polysciences, #23966) in Opti-MEM Reduced Serum Medium (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 0.2ml of 2% PFA + 2% glutaraldehyde (PolySciences) per well and subsequently stained with 0.2ml of crystal violet solution (8% crystal violet + 20% ethanol in ddH2O) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transiently transfected with plasmid DNA using PEI (Polysciences, 24765) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... for HeLa cells or Polyethylenimine (PEI) Max (Polysciences, Warrington, PA, USA) for 293T cells ...
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bioRxiv - Biochemistry 2023Quote: ... cells were co-transfected using PEI (PEI MAX MW 40,000, Polysciences) with the following plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected using polyethylenimine-MAX (PEI-MAX, PolySciences, 24765-1) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 250.000 cells were captured with BioMagPlus Concanavalin A (Polysciences, 86057-3) and incubated with the appropriate primary antibody overnight at 4°C (anti-Histone H3(tri methyl K4 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were transfected in growth medium using linear polyethyleneimine MAX (Polysciences) at a nitrogen/phosphate ratio of 20 and were used for experiments 24-48 hours later ...
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bioRxiv - Cancer Biology 2023Quote: ... were transfected into HEK293FT cells using 2 μg of Polyethylenimine (Polysciences). Lentiviral particle containing media was filtered using 0.45μm cellulose acetate syringe filter and used for transduction ...
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bioRxiv - Molecular Biology 2024Quote: ... All plasmids were cotransfected to HEK Expi293 cells by polyethylenimine (Polysciences) at 37°C for 72 hours ...
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bioRxiv - Microbiology 2020Quote: ... Transfection of EXPI293F cells was performed using PEI (Polyethylenimine HCl MAX; Polysciences). After 4 to 6 days of transfection ...
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bioRxiv - Neuroscience 2021Quote: ... were transfected into cells at 80%–90% confluency using polyethyleneimine (Polysciences, U.S.A.). We employed a pAAV ...
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bioRxiv - Microbiology 2019Quote: ... resuspended cells were applied to poly-L-lysine slides (Polysciences Inc., USA), dried for 5 minutes ...
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bioRxiv - Synthetic Biology 2019Quote: ... cells were transfected in DMEM supplemented with 5% FBS using polyethylenimine (Polysciences). Media was changed after 4 to 6 h and cells were stimulated with light starting 24 h after transfection for the durations specified below and at the intensities specified in the main text.
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bioRxiv - Cell Biology 2021Quote: ... the pigmentation of RPE cells was removed using Melanin Bleach Kit (Polysciences), followed by 3 washes with PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were washed and imaged in PBS and mounted with Aquapolymount (Polysciences) for storage.
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were reverse transfected using poly(ethyleneimine) (linear, 20 kDA; Polysciences)49 ...
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bioRxiv - Cancer Biology 2021Quote: Lentiviral particles were produced in HEK293T cells using polyethylenimine (PEI; Polysciences #23966) based transfection of plasmids ...
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bioRxiv - Microbiology 2021Quote: ... Cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently transferred to 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfection into HEK 293T cells was carried out using polyethylenimine (Polysciences) or Lipofectamine 2000 transfection reagent (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: HEK-293T cells were transiently transfected with plasmid DNA using polyethylenimine (Polysciences) at a ratio of DNA 1:3.4 polyethylenimine ...
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bioRxiv - Developmental Biology 2020Quote: ... coverslips with cells attached were mounted in Aqua-Poly/Mount (Polysciences, Inc.). Confocal stacks were collected using a Leica SP5 confocal microscope and processed by Fiji/ImageJ [61] and Adobe Photoshop software.
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... Yeast cells were then incubated with 0.01% Uvitex2B (a chitin marker; Polysciences) and mAb 18B7 (10 µg/ml ...