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Citations for Polysciences :
501 - 550 of 796 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected using Lipofectamine 3000 or Polyethylenimine (PEI) “MAX” reagent (Polysciences, Cat 24765), according to manufacturer’s protocols.
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bioRxiv - Immunology 2023Quote: HEK293T cells were cultured in 12-well plates and transfected by polyethyleneimine (PEI, Polysciences) with following constructs ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected using polyethylenimine (PEI; catalog no. 23966. Polysciences, Inc., Warrington, PA, USA) followed by culture medium exchange 4 h post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were transiently transfected with plasmid DNA mixed with PEI (Polysciences, Warrington, PA) and incubated for 5 days at 37 degC ...
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bioRxiv - Cell Biology 2022Quote: Transfection of HEK 293 cells with plasmid DNA was performed with PEI reagent (Polysciences) in a 1:1 (w/w ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells have been transfected using a home-made solution of PEI (MW 25000, Polysciences, stock concentration 0,324 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... the plasmid and helper plasmid were transfected into HEK293T cells using polyethylenimine (# 24765, Polysciences). The virus was harvested after 72 h and purified from benzonase-treated cell lysates via an iodixanol density gradient (OptiPrep ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells have been transfected using a home-made solution of PEI (MW 25000, Polysciences, stock concentration 0,324 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated for the binding with Concavalin A magnetic beads (Polysciences) by rotating for 10 min at RT ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid expressing PhK complex was transfected into the cells using polyethylenimine (PEI, Polysciences). After 36 hours of transfection ...
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bioRxiv - Biophysics 2023Quote: ... Expi293F cells were transfected with pHLsec- GPΔTM using polyethyleneimine (PEI MAX, Polysciences, Warrington, PA) at a mass ratio of 1:3 DNA:PEI MAX ...
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bioRxiv - Genetics 2023Quote: ... Briefly, HEK293T17 (ATCC CRL-11268, Manassas, VA, USA) cells were transfected using polyethylenimine (Polysciences) with the helper plasmid pxx-680 (a gift from R ...
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bioRxiv - Bioengineering 2024Quote: ... and delivered to HEK293T cells by transient transfection using linear 25 kDa polyethyleneimine (Polysciences). About 24 h post-transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with the corresponding plasmids using polyethylenimine (1 μg/μL [pH 7]; Polysciences) in a 1:3 ratio ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells growing at 50-70 % confluence were transiently transfected using linear polyethylenimine (M.W. 25000, Polysciences) as described (Chuykin et al. ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV-RC and pAAV-Helper plasmids into 293T cells using PEI MAX (Polysciences; Pennsylvania, USA). 72 h after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen sections and plated cells were mounted following antibody staining using AquaPolymount (Polysciences, Heidelberg, Germany) and imaged using a Zeiss Apotome ImagerZ2 (Zeiss ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T and HeLa cells were transiently transfected with plasmid DNA using polyethylenimine (PEI, Polysciences) at a final concentration of 5 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... and pMD2.G were co-transfected into 293T cells using polyethyleneimine (PEI, Polysciences 23966-1). Virus-containing supernatant media were harvested 48 hours post transfection and passed through 0.45 µm filters ...
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bioRxiv - Biophysics 2021Quote: ... RPE1 cells were transfected with 90 μL of Polyethylenimine (PEI MAX #24765 Polysciences, Warrington, PA) and 15 μg of the pX335-gRNA and pUC19-homology arms-iRFP plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... Briefly sub-confluent HEK 293 cells were transfected with branched or linear PEI (Polysciences, Inc.) 18 to 24 hrs after transfection the cells were lysed and incubated with CycB1 antibody overnight at 4°C and Protein G Sepharose beads (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells stably transduced with HSPB8 were transiently transfected using PEI MAX (24765-1, PolySciences Europe ...
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bioRxiv - Molecular Biology 2020Quote: ... YFP-TOP2B was transfected into HEK293F cells in Hyclone TransFx media (Cytiva) using PEI (Polysciences) and purified using the YFP-tag system (Schellenberg et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were washed with PBS and then post-fixed in 1.5% osmium tetroxide (Polysciences) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Cell Biology 2019Quote: ... into HEK 293T cells using 1mg/mL of PEI Max 40000 (Polysciences, cat. 24765-1). After 48 hours of transfection ...
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bioRxiv - Microbiology 2019Quote: ... and pVSV-G into 293T cells via polyethylenimine HCl MAX (PEI, Polysciences, Cat No: 24765) diluted in Opti-MEM (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... Transient transfections were performed 40-48 hr prior to cell lysis using polyethylenimine PEI (Polysciences) at around 60-70% confluence ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were incubated with 0.1 μg/mL DAPI and mounted in Poly mount (Polysciences; 18606).
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bioRxiv - Microbiology 2020Quote: ... Cells were then cross-linked with 1% formaldehyde (Ultra Pure EM grade, Polysciences, Warrington, PA), for 10 min at 26°C followed by another 5 min incubation after adding glycine to a final concentration of 125 mM to stop the cross-linking reaction ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences). For intracellular transcription factor staining ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences).
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bioRxiv - Bioengineering 2020Quote: ... The cells were transiently transfected with ACE2 plasmids and polyethylenimine (PEI) (Polysciences, Cat.24765-1) when the cell density reached approximately 1.0×106/mL ...
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bioRxiv - Biochemistry 2022Quote: LRRK2 R1441G was transfected into HEK293T cells with Polyethylenimine HCl MAX 4000 (PEI) (Polysciences, Inc.) and purified 48hrs post transfection ...
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bioRxiv - Biochemistry 2022Quote: ... adherent HEK293T cells were transfected with the expression plasmids using linear PEI (MW 25000, Polysciences). In detail ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviruses for Cas9 and sgRNAs were produced in 293T cells using standard polyethylenimine (Polysciences Inc.) transfection protocols.
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences) and acquired on a BD LSR Fortessa using BD FACS Diva.
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bioRxiv - Biochemistry 2023Quote: ... the cells were transfected with pCMV14-SMCT1 using PEI MAX pH 6.9 (MW 40,000; Polysciences) and cultured for two days.
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bioRxiv - Biophysics 2023Quote: ... Then the cells were pre-fixed for 1 min in 0.5% PFA (Formaldehyde, Polysciences, Inc) in PBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were transiently transfected with 200 ng MyD88-Venus construct using PEI Max (Polysciences) according to manufacturer’s protocol and were incubated for further 24 h at 37 °C in a 5% CO2 incubator ...
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bioRxiv - Neuroscience 2024Quote: N2A-Pz1-KO cells were transfected using PEI-Max (Polysciences Inc, Cat. Nu. 24765-100) with 1500 ng ptdTomato-N1 vector ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... or pMSCV-puro-based constructs were transiently transfected in HEK293T cells using polyethylenimine (Polysciences, 24765). For lentivirus production ...
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bioRxiv - Cell Biology 2024Quote: Equal numbers of HEK293T cells were seeded Wnt7a plasmid was transfected with linear polyethylenimine (Polysciences), accordingly to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... NIH3T3 cells were transfected with 1 μg/ml plasmid with polyethyleneimine (3 μg/ml; Polysciences, 23966). Media was changed the next day and cells harvested 48 hrs later ...
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bioRxiv - Cell Biology 2021Quote: ... MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.), cultured for 16 h ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stained with benzidine in combination with the Differential Quick Stain Kit (PolySciences, Warrington, PA) (manufacturers protocol) ...
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bioRxiv - Cancer Biology 2019Quote: Retrovirus was produced in Plat-E cells by transient transfection with PEI (Polysciences; Cat#23966-1) according to standard protocols ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected with plasmids encoding ACE2 orthologs or human DPP4 using PEI 40K (Polysciences) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The fixed and stained cells were washed with PBS and mounted using Aqua-Poly/Mount (Polysciences). Confocal images of infected cells were acquired using a Leica SP8 Scanning Confocal Microscope controlled by Leica-X software ...
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bioRxiv - Biochemistry 2022Quote: ... 30,000 cells per 48 well were plated and transfected with 1 μl PEI (Polysciences, PEI 25K), 75 ng of promoter reporter plasmids (3xMBS-pGL4.10 or pGl4.10 empty vector ...