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Citations for Polysciences :
301 - 350 of 426 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... then 1:2 mixture of propylene oxide and Polybed 812 epoxy resin for 4 hours and infiltrated overnight in 100% resin (08792-1, Polysciences). Samples were transferred to embedding molds and polymerized at 60□ overnight ...
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bioRxiv - Biochemistry 2021Quote: ... ~1.8 mg pCAG-ATP8B1 and ~0.3 mg pCAG-CDC50A/B were incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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bioRxiv - Bioengineering 2020Quote: ... The pore size of isotropic and anisotropic mineralized collagen scaffolds was analyzed following a JB-4 (Polysciences, Inc., Pennsylvania, USA) embedding procedure 30,47 ...
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bioRxiv - Neuroscience 2022Quote: ... 25 and 45 day old flies were immediately fixed after decapitation in 4% paraformaldehyde (Laborimpex) and 2% glutaraldehyde (Polysciences, Inc) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... helper plasmid in a 1:4:2 ratio by weight for a total of 40 µg mixed with PEI (40K MW #24765 Polysciences) in DPBS (Invitrogen 14190144) ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were washed twice with DPBS and fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc.) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mL expression medium were supplemented with 750 μg β2m-HLA-A*68:02Fos and 750 μg TAPBPRJun DNA (equimolar ratio) and mixed with a 4-fold molar excess of linear PEI (Polysciences) diluted in 5 mL expression medium ...
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bioRxiv - Neuroscience 2023Quote: ... and either fixed for 4 h in 4% PFA in PBS at 4 °C and cryopreserved in 20% sucrose in PBS overnight at 4 °C or directly cryopreserved and then frozen in OCT compound (Polysciences). Sections were prepared at 14 μm ...
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Brain-wide mapping of oligodendrocyte organization and oligodendrogenesis across the murine lifespanbioRxiv - Neuroscience 2024Quote: ... the tissue was washed 4 times with 20mM PB buffer and sections were mounted onto slides with Aqua Polymount (18606, Polysciences) and allowed to dry for 24-48 hours at 4°C prior to imaging ...
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bioRxiv - Neuroscience 2020Quote: ... L4 animals were immobilized using 0.05 µm polystyrene beads (Polybead Microspheres, Polysciences Cat #08691-10) or in 0.2mM Levamisole (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... 500,000 OSCs were bound to 10 µl Concanavalin A-coated magnetic beads (Polysciences, #86057-3) and lysed using Dig-wash buffer (20 mM HEPES pH 7.3 ...
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bioRxiv - Genetics 2020Quote: ... 200 µL of 1 µm Fluoresbrite yellow-green (YG) microspheres beads (#17154-10, Polysciences, Inc) diluted 1:4 in sterile DPBS were administered retro-orbitally ...
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bioRxiv - Genetics 2022Quote: ... The animals were immobilized by placing on a 10 % agarose pad with polystyrene beads (Polysciences), which were then covered by a cover slip ...
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bioRxiv - Neuroscience 2023Quote: 10 cm dish transfection: 9µg of plasmids and 25µL 1µg/µL Polyethylenimine (Polysciences, Warrington, PA) were mixed in 1mL Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 1µM-diameter fluorescent beads (Fluoresbrite® PolyFluor® 570 Microspheres, Polysciences, #24061-10) that were ingested by the worms ...
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bioRxiv - Developmental Biology 2020Quote: ... High resolution episcopic microscopy (HREM) was performed as previously described97 using an JB-4 embedding kit (00226-1, Polysciences GMBH, Germany). μMRI and HREM data were analysed using OsiriX MD DICOM viewer version 9.0.2 (Pixmeo) ...
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bioRxiv - Bioengineering 2020Quote: ... and 4 μg CD19-CAR-GFP transfer plasmid (Bloemberg et al. 2020) with 24 μg linear PEI (Polysciences Inc., Warrington, PA) for 6 hours ...
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bioRxiv - Bioengineering 2020Quote: ... and 10 ng of the TetR reporter plasmid pTS1017) was added into 300 μL DMEM and mixed with 4 μg of polyethyleneimine (Polysciences Inc.). The transfection mixture was vortexed and incubated for 20 min before adding into the inner 60 wells of the cell culture plate ...
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bioRxiv - Cell Biology 2022Quote: Adult fly heads were decapitated and immediately fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Bioengineering 2020Quote: The pore size of mineralized collagen and mineralized collagen-amnion scaffolds was analyzed following a JB-4 (Polysciences, Inc., Warrington, PA) embedding procedure (9,25) ...
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bioRxiv - Physiology 2021Quote: ... The 4-μm bone sections were stained with the von Kossa method and counterstained with tetrachrome (Polysciences Inc., Warrington, PA, USA). The 8-μm sections remained unstained to measure fluorochrome-based indices of bone formation.
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bioRxiv - Cell Biology 2022Quote: ... were transiently co-transfected with 4xPylT/PylRS(Y306A, Y384F) and 4xPylT/EMC3(Amber[TAG])-GFP plasmids at a ratio of 4:1 using PEI “MAX” (Polysciences, USA). The cells were grown in the presence of 0.5 mM AbK (Iris Biotech ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells at around 70% to 80% confluency in a 24-well plate were transfected with 500 ng/well of plasmid constructs expressing firefly luciferase using 4 ng/µl of polyethyleneimine (Polysciences, inc.). A plasmid expressing Renilla luciferase was co-transfected (50 ng/well) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... following by curing the resin overnight at 4°C in a plastic embedding mold (Cat no.-15899-50, Polysciences Europe GmbH, Germany). Polymerized sample blocks were further hardened by overnight baking at 70°C.
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Plant Biology 2020Quote: ... Dehydration was performed in an acetone series, followed by treatment with propylene oxide (SERVA, Heidelberg, Germany) at 4°C and embedding in Spurr’s resin (PolySciences, Niles, IL, USA). The embedded specimens were sectioned with a Reichert-Jung Ultracut E (Reichert-Jung Optical Company ...
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bioRxiv - Cell Biology 2022Quote: T24 cells were fixed in 2% glutaraldehyde and 2% paraformaldehyde at 4°C overnight and then postfixed in 2% osmium tetraoxide for 1.5 h at 4°C followed by dehydration with ethanol and embedding in Spurr resin (Polysciences Inc., Warrington, PA). Ultrathin sections of the samples were prepared using Ultramicrotome (Leica EM UC7 ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed samples were gradually dehydrated in an increasing series of ethanol concentrations and were embedded in a methacrylate resin (JB-4 kit, Polysciences, Warrington, PA) containing eosin and acridin orange as contrasts agents as previously described (Mohun and Weninger ...
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bioRxiv - Developmental Biology 2021Quote: ... Devitellinized embryos were transferred to a chilled mixture of 1% osmium tetroxide (Polysciences; Cat#23311-10) and 2% glutaraldehyde in 0.1 M cacodylate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... 10 µg pHelper and 6.5 µg pAAV plasmid per plate complexed with Max-polyethylenimine (PEI, Polysciences) at a PEI:DNA ratio (w/w ...
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bioRxiv - Biochemistry 2020Quote: A sample of lycopodium sporopollenin (50 mg in 10 mL acetic acid; Polysciences Inc., Warrington PA) was ozonized in a similar manner ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated at 37°C for 3 days and later fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... Histological sections of 10 μm thickness were obtained and EdU and picrosirius red staining (Polysciences, #24901) were performed according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... the 10 K fraction was calibrated with 150 nm diameter silica beads (24320, Polysciences, PA, USA), and 100 nm diameter beads (24041 ...
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bioRxiv - Plant Biology 2020Quote: Control and extract-treated (for 30 min or 1 h) root tips of 4- to 5-day-old rice seedlings were fixed in 3% (v/v) glutaraldehyde (PolySciences, Niles, IL, USA) in 50 mM sodium cacodylate buffer (pH 7 ...
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bioRxiv - Neuroscience 2024Quote: ... retinas were washed 4 times with PBS for 5 minutes prior to whole mounting onto slides with fluorescent mounting medium (Polysciences, Cat#18606-20). Slides were allowed to dry prior to imaging ...
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bioRxiv - Immunology 2024Quote: ... cells in each well were transfected with a total of 1 μg DNA (Env-expression plasmid and pCMV-rev expression helper plasmid in 4:1 ratio) mixed with 3 μg 25 kDa linear PEI (Polysciences Inc., Warrington, USA) in 200 μl 150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells were plated in two 10 cm2 dishes and transfected using PEI MAX (Polysciences, PA USA) with vRNP expression plasmids (3 µg/mL each of pCAGGS/PB2-FLAG ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with PBS for 10 minutes and mounted using AquaPolymount (Polysciences, Warrington, USA). Images were obtained on a Confocal and STED FACILITY line microscope (Abberior Instruments ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 10 mins incubation with 1 mL of 2 mg/mL Polyethylenimine (PEI) (Polysciences, POL23966-1). As next step ...
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bioRxiv - Neuroscience 2020Quote: ... Males were placed on a 10% agar pad and immobilized with 0.01 µm latex beads (Polysciences, Inc). Target neurons were identified based on the expression of fluorescent markers ...
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bioRxiv - Neuroscience 2020Quote: ... sections were subsequently washed with PBS 1X (3 × 10 min) and mounted in Aqua/polymount (Polysciences 18606).
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bioRxiv - Cancer Biology 2020Quote: ... complemented with 10% FCS and 2mM glutamine) were transfected using 4μg polyethylenimine (PEI, Polysciences Inc., Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μg of plasmid DNA was incubated with 100 μg/mL polyethyleneimine (Polysciences Inc; linear 25 kDa) in 500 μL DMEM without serum or antibiotic for 20 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated at 37°C for 3 days and then fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Cell Biology 2021Quote: ... 20µl of cells were spotted onto each well of a Teflon coated 10 well slide (Polysciences, 18357) that had been pre-treated with 10ng/µl poly-L-lysine ...
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bioRxiv - Neuroscience 2022Quote: Slices were washed 3 times for 10 minutes in PBS and mounted with Aqua-Poly/Mount (Polysciences). Images were taken using a Zeiss LSM780 confocal microscope and images were processed using ImageJ software ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM Spermidine) and bound to 10 µl activated BioMag®Plus Concanavalin A magnetic beads (Polysciences) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were sequentially infiltrated with increasing concentrations (10%, 25%, 50% and 75%) of Lowicryl HM20 (Polysciences, Hirschberg an der Bergstrasse ...