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Citations for Polysciences :
351 - 400 of 426 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were bound to concanavalin A beads (25 uL bead slurry per 100,000 nuclei, Polysciences, 86057-10), blocked with blocking buffer (20 mM HEPES-KOH ...
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bioRxiv - Biophysics 2024Quote: ... Tissue sections were sequentially infiltrated with increasing concentrations (10%, 25%, 50% and 75%) of Lowicryl HM20 (Polysciences, Hirschberg an der Bergstrasse ...
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bioRxiv - Biophysics 2024Quote: ... A total of 10 mL (1 mg/mL in PBS) of PEI Max 40 kDa (Polysciences Inc.) was added to the DNA and incubated for 20 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Neuroscience 2021Quote: ... washed again three times for 10 min in 0.1 M PBS and coverslipped with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Cell Biology 2022Quote: ... 20µl of cell suspension was spotted onto each well of a Teflon coated 10-well slide (Polysciences, 18357) that had been pre-treated with 10 ng/µl poly-L-lysine ...
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bioRxiv - Neuroscience 2020Quote: ... Finally sections were subsequently washed with PBS (3 x 10 min) and mounted in Aqua/polymount (Polysciences, 18606).
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bioRxiv - Microbiology 2021Quote: ... into 70% confluent monolayer of 293T cells in a 10 cm dish using polyethylenimine (Polysciences Inc, Warrington, PA.) at 4X total transfected DNA in 800 μl 150 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... washed again three times for 10 min in 0.1 M PBS and coverslipped with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed three times in PBS for 10 minutes and coverslipped with Aqua-Poly/Mount (Polysciences, Inc.) mounting media.
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bioRxiv - Neuroscience 2022Quote: ... were administered fluorescent polystyrene latex nanoparticles (Fluoresbrite YG; 200 nm diameter) (Polysciences, Warrington, PA; cat. No. 09834-10). After 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... The anterior or posterior legs were cut from the thorax and fixed in 10% formaldehyde (Polysciences Cat# 04018) overnight (14–16 h ...
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bioRxiv - Biophysics 2024Quote: ... Cells were transfected using a polyethylenimine (PEI) agent (10 μl of 1 mg/ml per dish hereafter; Polysciences) with pCAG plasmids encoding human A1R ...
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bioRxiv - Bioengineering 2023Quote: ... separate samples of 10 kPa solution were made substituted with Nile blue-tagged acrylamide (Polysciences Inc. #25395-100) at 1:1000.
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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Novel amiloride derivatives that inhibit bacterial motility across multiple strains and stator typesbioRxiv - Microbiology 2021Quote: ... and incubated for 10 mins and washed with motility buffer and flushed with 1 μm polystyrene bead (Polysciences, Inc) solution ...
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bioRxiv - Neuroscience 2021Quote: ... Day 1 adults were placed on a 10% agarose pad and immobilized with a slurry of polystyrene beads (Polysciences). A 1KHz train of 100 fs pulses (15-30 nJ/pulse ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were dissected on a cover glass in a drop of 65% Shelton’s Growth Medium (SGM) with 35% Fetal Calf Serum containing 10 µm polystyrene spacer beads (Polysciences). Subsequently a glass slide was lowered over it such that the embryos were confined between the cover glass and the glass slide ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T cells in a 10 cm dish were transfected with 40 µL 1.0 mg/mL polyethylenimine (Polysciences, Warrington, PA) and 15 µg plasmid and incubated for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were collected and mounted in 8-10 μL of egg buffer supplemented with 18.8μm polystyrene beads (Polysciences, Inc.).
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bioRxiv - Microbiology 2021Quote: Prior to flow cytometry analysis 2 μl of fluorescent microspheres (1×10−3 of the stock Fluoresbrite® YG Microspheres 0.75μm, Polysciences) was added and the volume was adjusted to 500 μl by adding FACSFlow solution (10 mM phosphate-buffered saline ...
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bioRxiv - Physiology 2022Quote: ... Slides were first stained in Weigert’s Iron Hematoxylin solution for 10 min at room temperature (Polysciences Inc., Warrington, PA). This was followed by 2 min wash in tap water ...
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bioRxiv - Molecular Biology 2022Quote: L1 and L4 larvae were mounted on a 10% agarose pad and immobilized in a 1-2 μL suspension of 0.1 μm polystyrene beads (Polysciences), as described in (Kim et al ...
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bioRxiv - Developmental Biology 2021Quote: ... elegans larvae were mounted on a 10% agarose pad in a solution of 100 nm polystyrene microbeads (Polysciences Inc.) to stop worm movement ...
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bioRxiv - Microbiology 2023Quote: ... containing 10 µL of PEI reagent (2 mg/mL concentration of PEI MAX MW 40,000: Polysciences, Warrington, PA, USA) and then transfected into 293T cells grown to confluency in 6-well plates ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in wash buffer and 10 µl BioMag®Plus Concanavalin A (ConA) beads (Polysciences, 86057-3) were added for 10 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in a sterile aqueous suspension without sodium azide ...
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bioRxiv - Microbiology 2024Quote: ... Transient DNA transfections were performed using linear poly(ethylenimine) (1 mg/mL of solution in 10 mM Tris-HCl, pH 6.8; Polysciences), Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... pEGFP-N1-RHBDL4 was transfected as a 1:10 mix with empty pcDNA3.1 plasmid into Hek293T cells (ATTC) using 25-kDa linear polyethylenimine (Polysciences) as had been described (Knopf et al ...
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Microplastic consumption induces inflammatory signatures in the colon and prolongs a viral arthritisbioRxiv - Immunology 2021Quote: The MPs comprised Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1.00 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in aqueous suspension without sodium azide ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 48 hours at 37°C after which the media was removed and 150 uls of 10% formaldehyde (Polysciences) was added to inactivate the virus ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Neuroscience 2020Quote: ... One-day-old adults from these strains were mounted on a 10% agarose pad with 1.6 µL of a mixture containing equal measures of polystyrene beads (Polybead, Polysciences Inc.) and 30 mM muscimol (MP Biomedicals) ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and the slides were mounted with Aqua-Poly/Mount (Polysciences).
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... 5 μg of an HIV-1 Gag-Pol expression plasmid (pCRV1/GagPol) and 1 μg of VSV-G expression plasmid in 293T cells in 10-cm dishes using polyethylenimine (PolySciences). Virus-containing supernatant was collected and filtered (0.2μm ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C for 72 hours after which cells were fixed with 1 ml of 10% formaldehyde (Polysciences) was added for 24 hours to ensure inactivation of virus ...
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bioRxiv - Immunology 2020Quote: Cell numbers per tissue were determined by mixing 20 µl of each single cell suspension into a 96-well plate with 50 μl of 8.4 × 104 Fluoresbrite Carboxylate YG 10 µm microspheres/ml (Polysciences Inc.) and 180 μl staining media (dPBS + 2% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... and then transfected with 0.75 μg of an equimolar mixture of the three third-generation packaging plasmids (pMD2.G, psPAX2, pMDLg/pRRE) and 0.75 μg of LSR vectors using 10 μl of polyethylenimine (PEI, Polysciences #23966) and 200 μl of cold serum free DMEM ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA with the amount of 10 μg was mixed with 30 μg of linear polyethyleneimine (PEI) (23966-100; Polysciences) in 1 ml of serum-free DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... were used The slides were again washed and stained with DAPI (Thermo-Fisher, 300 nM) for 10 min prior to mounting with Aqua PolyMount medium (PolySciences).
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bioRxiv - Cell Biology 2022Quote: ... embryos were dissected in 8-10 μL of Shelton’s Growth Medium (Edgar and Goldstein, 2012) and mounted with 20 μm polystyrene beads (Polysciences, Inc.) between a slide and coverslip ...
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bioRxiv - Molecular Biology 2023Quote: ... freshly seeded 293T cells grown in DMEM supplemented with 10% FBS + 50 µg/mL gentamycin at 37°C with 5% CO2 (hereafter, supplemented DMEM) were transfected using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 48 hours prior to fixation with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Cancer Biology 2024Quote: Poly(methyl methacrylate-co-methacrylic acid) (PMMA-MA; 10% methacrylic acid, MW ∼100,000 Da, 17913-500) was from Polysciences (USA), organic solvents were obtained from Carl Roth (Austria) ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed three times in PBS for 10 minutes and coverslipped using 2 drops of Aqua-Poly/Mount (Polysciences, Inc.) as the mounting media.
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bioRxiv - Neuroscience 2021Quote: ... The surface of the substrate was plasma treated at 500 W for 5 min and coated with an aqueous solution of 10% (wt/vol) Poly(acrylic acid) (MW 50000, Polysciences, USA) at 2000 rpm for 15 s using a spin-coater (WS-650-23 ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mm dishes of HEK293T cells (∼80% confluent) were transfected with 10 μg of plasmid using 60 μg of linear Polyethylenimine 25 kDa (23966, Polysciences Inc) mixed in 2 mL Opti-MEM (31985070 ...
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bioRxiv - Cell Biology 2022Quote: ... worms were mounted on 8–10% agarose pads in 3 μL of a 0.5 μM polystyrene bead slurry (Polysciences, Warrington, PA) in PBS pH 7.4 (Kim et al. ...