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Citations for Polysciences :
251 - 300 of 840 citations for 4 15 Oppositene 1 7 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and 1 mg/mL polyethylenimine (PEI) (Polysciences, 23966-1) in a 4:1 ratio of DNA (μg):PEI (μg) ...
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bioRxiv - Bioengineering 2023Quote: ... and pAAV for in a 1:1:1 molar ratio using polyethylenimine (PEI, Cat# 23966, Polysciences). The AAV production media were collected 72 h and 120 h post transfection and concentrated using a PEG-precipitation method with 8% PEG-8000 (wt/vol ...
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bioRxiv - Cell Biology 2023Quote: Transfections were done transiently using PEI transfection reagent (1 mg ml-1 stock, Polysciences, 24765-1), 4 µg of transfection reagent per 1 µg of plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 3:2:1 using 1 mg/ml polyethylenimine (Cat#23966-1, Polysciences, Inc.). Retrovirus was harvested from the medium 48 h post-transfection ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
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bioRxiv - Developmental Biology 2021Quote: Adult guts were dissected in PBS and fixed twice during 20 min in fresh 4% paraformaldehyde diluted in PBS (18814-10, Polysciences) with a 10 min wash in PBT 0,1% (PBS + Triton X100 Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 2 µg of pCI-neo Fluc-EGFP or pCI-neo FlucDM-EGFP with 4 µg of PEI (Polysciences) and cultured for an additional 24 h ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells grown in 60 mm dish to 70-80 % density were transfected with a mixture of 4 μg plasmid DNA and 20 μg polyethylenimine (Polysciences). Cell lysates were extracted 24 hrs after transfection.
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bioRxiv - Cell Biology 2022Quote: ... Samples were then stained with 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) for two minutes to visualize nuclei and mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Biochemistry 2021Quote: ... ~1.8 mg pCAG-ATP8B1 and ~0.3 mg pCAG-CDC50A/B were incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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bioRxiv - Bioengineering 2020Quote: ... The pore size of isotropic and anisotropic mineralized collagen scaffolds was analyzed following a JB-4 (Polysciences, Inc., Pennsylvania, USA) embedding procedure 30,47 ...
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bioRxiv - Neuroscience 2022Quote: ... 25 and 45 day old flies were immediately fixed after decapitation in 4% paraformaldehyde (Laborimpex) and 2% glutaraldehyde (Polysciences, Inc) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were washed twice with DPBS and fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc.) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mL expression medium were supplemented with 750 μg β2m-HLA-A*68:02Fos and 750 μg TAPBPRJun DNA (equimolar ratio) and mixed with a 4-fold molar excess of linear PEI (Polysciences) diluted in 5 mL expression medium ...
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bioRxiv - Biophysics 2022Quote: ... 100 μg mL-1 streptomycin and 5% CO2 atmosphere at 370 C were transiently transfected with wild type human and rat NTSR1 in pcDNA3.1 (4 μg DNA per 75% confluent 10 cm dish) via hybridization with polyethylenimine (PEI, Polysciences, Inc.). The following day ...
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bioRxiv - Neuroscience 2023Quote: ... and either fixed for 4 h in 4% PFA in PBS at 4 °C and cryopreserved in 20% sucrose in PBS overnight at 4 °C or directly cryopreserved and then frozen in OCT compound (Polysciences). Sections were prepared at 14 μm ...
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Brain-wide mapping of oligodendrocyte organization and oligodendrogenesis across the murine lifespanbioRxiv - Neuroscience 2024Quote: ... the tissue was washed 4 times with 20mM PB buffer and sections were mounted onto slides with Aqua Polymount (18606, Polysciences) and allowed to dry for 24-48 hours at 4°C prior to imaging ...
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bioRxiv - Biochemistry 2021Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1% glutaraldehyde (Polysciences) in 0.1 M cacodylate buffer (Polysciences ...
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bioRxiv - Neuroscience 2022Quote: ... AAV7A2 and AAV9A2 caps were used in this study) and adenoviral helper plasmid at a 1:1:1 molar ratio using a polyethylenimine (PEI MAX; Polysciences, Warrington, USA, cat.no. 24765-1) as the transfection reagent ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Microbiology 2020Quote: ... we introduced 1 mL of 1×109 / mL 1 µm diameter YG carboxylate fluorescent microspheres (Polysciences, Warrington, PA) to the overlying water of a LSSM sediment core ...
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bioRxiv - Cell Biology 2020Quote: ... 900 μL 1 mg/mL PEI MAX 40k (Polysciences, 24765-1) and 300 μg DNA ...
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bioRxiv - Neuroscience 2023Quote: 1% Fast Blue in sterile dH2O (17740-1, Polysciences, Warrington, PA) was used as a fluorescent retrograde neuronal tracer to identify gastrointestinal-projecting sensory neurons using a modified method as previously described (76) ...
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bioRxiv - Bioengineering 2020Quote: ... and 4 μg CD19-CAR-GFP transfer plasmid (Bloemberg et al. 2020) with 24 μg linear PEI (Polysciences Inc., Warrington, PA) for 6 hours ...
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bioRxiv - Neuroscience 2021Quote: Mice were sacrificed at 2 and 10 months of age by perfusion with 4% w/v paraformaldehyde (PFA; Polysciences, EM grade) and 0.1% v/v glutaraldehyde (GA ...
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bioRxiv - Bioengineering 2020Quote: ... and 10 ng of the TetR reporter plasmid pTS1017) was added into 300 μL DMEM and mixed with 4 μg of polyethyleneimine (Polysciences Inc.). The transfection mixture was vortexed and incubated for 20 min before adding into the inner 60 wells of the cell culture plate ...
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bioRxiv - Cell Biology 2022Quote: Adult fly heads were decapitated and immediately fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Bioengineering 2020Quote: The pore size of mineralized collagen and mineralized collagen-amnion scaffolds was analyzed following a JB-4 (Polysciences, Inc., Warrington, PA) embedding procedure (9,25) ...
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bioRxiv - Physiology 2021Quote: ... The 4-μm bone sections were stained with the von Kossa method and counterstained with tetrachrome (Polysciences Inc., Warrington, PA, USA). The 8-μm sections remained unstained to measure fluorochrome-based indices of bone formation.
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bioRxiv - Molecular Biology 2024Quote: ... Cells at around 70% to 80% confluency in a 24-well plate were transfected with 500 ng/well of plasmid constructs expressing firefly luciferase using 4 ng/µl of polyethyleneimine (Polysciences, inc.). A plasmid expressing Renilla luciferase was co-transfected (50 ng/well) ...
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bioRxiv - Cell Biology 2023Quote: ... and HA-ApoER2 or FLAG-ApoER2 at a 1:1:1 ratio in DMEM using polyethylenimine (PEI 25k, Polysciences). After 3-4 h of transfection ...
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bioRxiv - Neuroscience 2020Quote: ... and light chains (CA5-VL(X3)_pHL_sec) at a 1:1 ratio using polyethyleneimine (PEI, 1 mg/ml, MW 40,000, Polysciences Cat.# 24765) transfection reagent ...
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bioRxiv - Biophysics 2023Quote: ... the cells were transfected with WT hVMAT2-eGFP or the mutation plasmids (2μg plasmid per construct) at a ratio of 4μL polyethylenimine (PEI, 1 μg μL−1, Polysciences, 24765-1) per μg of DNA ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... along with 10 uL of 1 mg/mL PEI (Polysciences 24765-1) in 200 uL of serum-free DMEM ...
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bioRxiv - Biochemistry 2024Quote: ... 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences, #23966-1) diluted in 18.8 mL Opti-MEM® ...
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bioRxiv - Evolutionary Biology 2022Quote: ... following by curing the resin overnight at 4°C in a plastic embedding mold (Cat no.-15899-50, Polysciences Europe GmbH, Germany). Polymerized sample blocks were further hardened by overnight baking at 70°C.
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bioRxiv - Cell Biology 2021Quote: Samples were crosslinked for 10 min at RT and 20 min at 4°C with 0.8% formaldehyde (methanol free, ultrapure EM grade, Polysciences, Inc; Warrington PA). Reaction was stop at RT for 10 min with the addition of Glycine to a final concentration of 0.125 M ...
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bioRxiv - Plant Biology 2020Quote: ... Dehydration was performed in an acetone series, followed by treatment with propylene oxide (SERVA, Heidelberg, Germany) at 4°C and embedding in Spurr’s resin (PolySciences, Niles, IL, USA). The embedded specimens were sectioned with a Reichert-Jung Ultracut E (Reichert-Jung Optical Company ...
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bioRxiv - Cell Biology 2022Quote: T24 cells were fixed in 2% glutaraldehyde and 2% paraformaldehyde at 4°C overnight and then postfixed in 2% osmium tetraoxide for 1.5 h at 4°C followed by dehydration with ethanol and embedding in Spurr resin (Polysciences Inc., Warrington, PA). Ultrathin sections of the samples were prepared using Ultramicrotome (Leica EM UC7 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed with fresh 3% paraformaldehyde at 4°C for 10 mins and smeared on poly-L-lysine pre-coated slides (Polysciences, Inc., USA). Cells were washed once with 1× PBS and incubated with 100 times dilution of BODIPY® FL vancomycin (Van-FL ...
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bioRxiv - Cell Biology 2024Quote: Midguts were dissected in 1X PBS (Oxoid, BR0014G) and fixed for 20 minutes at room temperature in fresh 4% methanol-free paraformaldehyde (Polysciences, 18814-10) diluted in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed samples were gradually dehydrated in an increasing series of ethanol concentrations and were embedded in a methacrylate resin (JB-4 kit, Polysciences, Warrington, PA) containing eosin and acridin orange as contrasts agents as previously described (Mohun and Weninger ...
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bioRxiv - Cell Biology 2021Quote: ... PEI (Polysciences, 23966-1) or lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Acetic Acid (Polysciences) was pipetted directly onto the sections for one minute ...
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bioRxiv - Molecular Biology 2023Quote: ... (PEI; Polysciences, 24765-1), co transfection were at 1:1 (4 µg:4 µg) ...