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Citations for Polysciences :
101 - 150 of 840 citations for 4 15 Oppositene 1 7 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... and 800 ng of the desired pLenti CMV Puro DEST or pLenti-TRE-rtta3G-BLAST construct using 7 μl of 1mg/ml polyethylenimine (PEI) (Polysciences). Approximately 48 hours post transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... Day-one adults were mounted with 100 nm polystyrene beads (Polysciences #00876-15; 5-fold dilution) on an agarose pad ...
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bioRxiv - Neuroscience 2024Quote: ... The worms were immobilized using 0.2 μl of 0.1 μm diameter polystyrene beads (00876-15; Polysciences) in 5% agarose pads (Basu et al ...
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bioRxiv - Biochemistry 2020Quote: ... For transfection cells were centrifuged and resuspended in fresh media to a density of 4×106 cells/mL and transfected with 4 μg plasmid/mL and 16 μg/mL of PEI 40 kDa (Polysciences). 4 h up to 24 h after transfection cells were fed with 75% of the transfection volume ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were mounted in Mowiol 4-88 (Polysciences) and imaged on a Zeiss confocal microscope (LSM 880) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed with 4% paraformaldehyde (PFA; Polysciences) for 10 minutes at 37°C and permeabilized in ice-cold 90% methanol (Merck ...
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bioRxiv - Cell Biology 2021Quote: ... Dissected midguts were fixed in 4% Formaldehyde (Polysciences) in 1x PBS for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... and fixed in 4% methanol-free formaldehyde (Polysciences) and 0.1 M potassium phosphate buffer (pH 7.4 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Sections were poststained with 4 % uranyl acetate (Polysciences) in 25% ethanol and ‘Reynold’s lead citrate’ ...
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bioRxiv - Evolutionary Biology 2024Quote: Dissected guts were fixed with 4% formaldehyde (Polysciences), 1% glutaraldehyde (Polysciences ...
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bioRxiv - Plant Biology 2020Quote: Control and extract-treated (for 30 min or 1 h) root tips of 4- to 5-day-old rice seedlings were fixed in 3% (v/v) glutaraldehyde (PolySciences, Niles, IL, USA) in 50 mM sodium cacodylate buffer (pH 7 ...
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bioRxiv - Immunology 2024Quote: ... cells in each well were transfected with a total of 1 μg DNA (Env-expression plasmid and pCMV-rev expression helper plasmid in 4:1 ratio) mixed with 3 μg 25 kDa linear PEI (Polysciences Inc., Warrington, USA) in 200 μl 150 mM NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed 3 × 30 mins in PBS and then immersed through a gradient of sucrose solutions from 10%-50% at 4°C for > 4 hrs each before being embedded in cryomolds (Polysciences 18646A) using OCT (Sakura Finetek ...
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bioRxiv - Cell Biology 2022Quote: Live animals were mounted on 10% agarose pads and immobilized using 0.1um polystyrene microspheres (Polysciences 00876-15) [45] ...
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bioRxiv - Cell Biology 2022Quote: ... the COs were fixed with 4% paraformaldehyde (PFA; Polysciences) for 30 min at room temperature and subsequently embedded in cryogel (Tissue-Tek ® O.C.T ...
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bioRxiv - Developmental Biology 2021Quote: ... and fixed in 4% formaldehyde (Polysciences, Inc., 18814-10) in 1xPBS for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) for 10 min on ice before and then incubated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... and fixed in 4% formaldehyde (Polysciences, Inc., 18814-10) in 1xPBS for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in 4% methanol-free formaldehyde (PolySciences) in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Testes were fixed in 4% formaldehyde (Polysciences 18814-20) in PBT (PBS + 0.2% Triton-X 100 (Sigma 108603) ...
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bioRxiv - Biochemistry 2021Quote: ... Stable cell lines expressing N-terminal Flag tagged receptor constructs in pcDNA3.1 vector were generated by transfecting HEK-293 cells with 7 μg of plasmid DNA using polyethylenimine (PEI) (Polysciences, Cat. no. 19850), followed by selection using G418 (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transfected with 5 µg plasmid DNA and 15 µg Polyethylenimine (PEI 25K™, Polysciences, 23966-100), both previously prepared in OptiMEM (Gibco ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were fixed in 4% glutaraldehyde (Polysciences; Cat#18428-5) and 2% acrolein (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... followed by fixation using 4% paraformaldehyde (18814-20, Polysciences Inc.) in 1X PBS for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in PBS with 4% formaldehyde (Polysciences, Inc.) and 0.02% glutaraldehyde for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... 1.5 mg plasmids and 4 mg inear polyethylenimines (PEI) (Polysciences) were pre-incubated in 50 ml fresh medium for 30 min before adding into one litre cells at density of 3.0 × 106 cells ml−1 ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were fixed by adding 100 μl 4% paraformaldehyde (Polysciences) for 15 min ...
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bioRxiv - Systems Biology 2023Quote: ... transferred to a pre-fix solution containing 4% formaldehyde (PolySciences) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Ovaries were embedded in JB-4 Plus plastic resin (Polysciences) and 5-10 μm sections were cut using a Leica RM 2155 microtome ...
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bioRxiv - Biochemistry 2022Quote: ... dissected midguts were immediately placed in fixative (4% formaldehyde (Polysciences), 0.2% saponin (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... cells were then fixed in 4% formaldehyde (Polysciences, Inc., 18814) for 15 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... fixed in 4% formaldehyde (16% stock solution, PolySciences 18814-20) in PBS for 25mn at R.T. ...
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bioRxiv - Cell Biology 2022Quote: ... cells were pre-permeabilised in 0.5% Triton X-100 in cytoskeleton buffer for 15 s and then fixed in 0.5% glutaraldehyde (no.00216-30; Polysciences) in cytoskeleton buffer with 0.5% Triton X-100 and 10% sucrose for 15 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: In utero live imaging of oocytes was accomplished by mounting young adult worms with single row of embryos to a 5% agarose pad on a 24×40mm glass coverslip with 1.5 µl each of 15°C M9 buffer and 0.1 µm polystyrene microspheres (Polysciences Inc.), and were gently covered with a 18×18mm coverslip ...
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bioRxiv - Biophysics 2023Quote: Cells were pre-permeabilized in 0.5% Triton X-100 in cytoskeleton buffer for 15 s for tubulin and then fixed in 0.5% glutaraldehyde (no.00216-30; Polysciences) in cytoskeleton buffer with 0.5% Triton X-100 and 10% sucrose for 15 min at room temperature ...
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bioRxiv - Genetics 2023Quote: ... We resuspended pupal abdomens in 15 ul of BioMag Plus Concanavalin-A-conjugated magnetic beads (ConA beads, Polysciences Inc.) and followed a CUT&RUN protocol for Drosophila tissues (https://dx.doi.org/10.17504/protocols.io.umfeu3n) ...
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bioRxiv - Neuroscience 2023Quote: ... Worms were mounted with cover glass on 5% agarose pads with a drop of polystyrene beads (Polysciences 00876-15) as a friction raising agent ...
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bioRxiv - Genetics 2024Quote: ... 1 million HEK 293T cells in a single well of a 6-well plate were transfected using PEI-Max MW 40,000 (PolySciences, CAS Number: 49553-93-7) mixed with 1 μg of PsPax2 (Addgene # 12260) ...
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bioRxiv - Microbiology 2020Quote: Subconfluent 293T cells in a T75 flask were transfected with 15 μg of the SARS-CoV-2 S plasmid using polyethylenimine (Polysciences), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
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bioRxiv - Systems Biology 2022Quote: ... and 750ng of lentiviral transfer plasmid encoding the transgene of interest after a 15 minute incubation of these plasmids with 10μL of polyethylenimine (PEI, Polysciences #23966). Lentivirus-containing culture supernatant was harvested 72 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... on a microscope slide with 1.6 µL of a solution containing equal measures of polystyrene beads (Polybead CAT No. 00876-15, Polysciences Inc.) and 30 mM muscimol (CAT No ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were mounted using 10% (wt/vol) Mowiol 4-88 (Polysciences). Epifluorescent images were captured using a Nikon Eclipse NiE (40×/0.75 Plan Fluor Nikon objective ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were mounted with Mowiol 4–88 antifade medium (Polysciences Europe). Fixed neurons were imaged on a confocal laser-scanning microscope Leica TCS SP8 with identical settings used for all samples ...
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bioRxiv - Neuroscience 2020Quote: ... brain organoids were placed directly in 4% methanol-free formaldehyde (Polysciences) diluted in DPBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). To block unspecific binding sites the fixed cells were incubated with blocking buffer containing 10% normal goat or donkey serum ...
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bioRxiv - Cell Biology 2021Quote: ... eyes were embedded in glycol methacrylate A resin (JB-4, Polysciences) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). Unspecific binding sites were blocked by incubation with blocking buffer ...