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Citations for Polysciences :
101 - 150 of 742 citations for Rat Heat Shock 60 kDa Protein 1 Chaperonin HSP60 HSPD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cells with a concentration of 106 cells/mL were transfected with 1.5 mg of DNA per liter of the culture using transfection reagent 40 kDa PEI MAX (Polysciences, Inc.) in a 1:2 ratio ...
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bioRxiv - Biochemistry 2024Quote: ... were transfected between 50% and 70% confluence with 8 µg of plasmid DNA/ 14 cm culture dish using polyethyleneimine (PEI) 25 kDa (Polysciences), and cultured post-transfection for 48 hrs in 14 cm dishes in DMEM (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... The light and heavy chain expression plasmids of LH03 or LH05 were mixed with 25 kDa linear polyethylenimine (PEI, Polysciences) and transiently transfected in HEK293E cells ...
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bioRxiv - Microbiology 2023Quote: The 25kDa PEI 25™ (PEI-25) and 40 kDa PEI Max® (PEI-40) polymers were acquired from Polysciences and were prepared according to manufacturer instructions to get a stock solution of 1 mg/ml ...
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bioRxiv - Systems Biology 2023Quote: Retrovirus was produced by transiently co-transfecting HEK 293T cells with a plasmid encoding a CAR or control construct and pRD114/pHIT60 virus-packaging plasmids (courtesy of Dr. Steven Feldman of the National Cancer Institute) using linear polyethylenimine (25 kDa; Polysciences). After 48 and 72 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Cell Biology 2024Quote: ... Polybead® Polystyrene Sampler Kit (19822-1) was purchased from Polysciences. Anti-GFP (11814460001 ...
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bioRxiv - Biophysics 2021Quote: ... in a microcentrifuge tube and resuspending in coupling buffer (400 μl in first wash and 170 μl in second washing) (PolyLink Protein Coupling Kit, Polysciences Inc.). Then 20 μl of the freshly prepared EDCA solution (20 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected at a density of 2.5 × 106 / ml with a DNA concentration of 3 μg/ml and polyethyleneimine (Linear PEI 25 kDa molecular weight, PolySciences Inc) at 9 μg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with IRE1 expression plasmids or pcDNA4 as control (500 ng per well) using PEI (linear 25 kDa, Polysciences, Inc.) at a DNA:PEI mass ratio of 1:3 ...
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bioRxiv - Neuroscience 2022Quote: ... The seeded cells were then transfected with a total 2 μg of DNA using polyethylenimine 25 kDa (PEI, Polysciences Inc., Germany) at a 6:1 ratio of PEI to DNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Slides were stained using a Masson Trichrome stain kit (Polysciences, 25088-1) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The day after seeding, pcDNA3L-His-CAMYEL plasmid were transiently transfected into cells using linear polyethyleneimine (PEI, m.w. 25 kDa) (#23966, Polysciences, Warrington, PA, USA). The DNA-PEI complex mixture was added into the cells at the ratio of 1:6 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were transiently transfected with 5 μg of pcDNA3L-His-CAMYEL plasmid using the linear polyethylenimine (PEI, m.w. 25 kDa) (Polysciences, Warrington, PA, USA). The PEI/DNA complex mixture was sequentially added to the cells at the ratio of 1:6 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Canada) and transiently co-transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimin (PEI; Polysciences Inc., USA). PEI was dissolved in water as 1 mg/mL stock solution with pH of 6.5–7.5 and kept at −20°C ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection was initiated by the addition of plasmid DNA and polyethyleneimine as transfection reagent (linear 25-kDa polyethyleneimine, Polysciences, Inc., Warrington, PA). Twenty-four hours post-transfection the cell cultures were diluted with an equal volume of fresh media supplemented with valproic acid (2.2 mM final concentration ...
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bioRxiv - Microbiology 2020Quote: ... pCAG-HIVgp (5 μg) and pCMV-VSV-G-RSV-Rev (5 μg) (Riken BioResource Research Center) using PEImax solution (60 μL) (Polysciences) prepared in KnockoutDMEM/F-12 (500 μL ...
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bioRxiv - Synthetic Biology 2024Quote: ... one day after plating 2ug of each plasmid (maximum 8 ug of plasmid) was transfected into cells using 60 ug of PEI max (Polysciences). Producer cells were then maintained for 3 days after transfection ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 1 µg per one million cells of the plasmids containing CMV promoter and 3×FLAG-tagged geneswere mixed with a three-fold excess (w/w) of polyethylenimine MAX 40 kDa (Polysciences, Warrington, PA, USA) in 4 ml OptiPro (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... were diluted with 150 mM NaCl to a concentration of 40 μg/mL and mixed with 40-kDa polyethyleneimine (PEI, Polysciences, Warrington, PA, USA) (DNA ...
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bioRxiv - Genomics 2022Quote: ... aligned nanofibrous dual-component scaffolds were developed with approximately a 50:50 ratio of PCL and water-soluble polyethylene oxide (PEO; 200 kDa, Polysciences, Inc., Warrington, PA). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 embryos (1.5-2 h AEL) or 200 embryos (60-80 min AEL) were incubated with 30 μl of BioMag®Plus Concanavalin A beads (Polysciences) (prepared in Binding buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells were seeded to achieve 50–60% confluence the following day and transfected using PEI (polyethylenimine, Polysciences Inc., Cat# 23966) as follows ...
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bioRxiv - Cancer Biology 2021Quote: High titer lentivirus was generated by transfecting HEK293FT cells with a mixture containing three plasmids and 25-kDa linear polyethyleneimine (PEI) (Polysciences, Inc. PA, USA, 239662). In brief ...
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bioRxiv - Cell Biology 2022Quote: ... For large scale transfections of the adherent HEK293T cells for the ectopic expression of proteins to be used for the NB in vitro assembly experiments, we followed a transfection protocol (Reed et al, 2006) using linear 25 kDa polyethylenimine (PEI; Polysciences Europe GmbH, Hirschberg, Germany). We adjusted this protocol for 10 cm culture dishes with about 50-70% cell density ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Montreal, Canada) and transiently transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimine (Polysciences Inc., Warrington, PA, US) in Cellstar® PS 96-well cell culture plates (Greiner Bio-One ...
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bioRxiv - Neuroscience 2024Quote: ... FreestyleTM 293F cells were transiently transfected with the mixture of DNA plasmid and polycation polyethylenimine (PEI; 25 kDa linear PEI, Polysciences, Inc., cat. No. 23966). The transfected cells were incubated at 125 rpm in FreestyleTM 293 Expression Medium for suspension culture at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2020Quote: 0.75 μm diameter fluorescent beads (Fluoresbrite YG Carboxylate Kit I 21636-1, Polysciences Inc.) were suspended at a concentration of 0.04% (w/w ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were embedded using the Poly/Bed 812 Embedding Kit (Polysciences, #08792-1) and sectioned to a thickness of 200 to 250 nm ...
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bioRxiv - Cancer Biology 2024Quote: ... CRUK0764 were labelled with CellVue® Red Mini Kit for Membrane Labeling (Polysciences, 25567-1) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... These vectors along with lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK293FT cells by polyelthylenimine (Polysciences, Inc; 60 μg/mL) mediated transfection ...
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bioRxiv - Biophysics 2022Quote: ... 100 μg mL-1 streptomycin and 5% CO2 atmosphere at 370 C were transiently transfected with wild type human and rat NTSR1 in pcDNA3.1 (4 μg DNA per 75% confluent 10 cm dish) via hybridization with polyethylenimine (PEI, Polysciences, Inc.). The following day ...
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bioRxiv - Cell Biology 2020Quote: ... washed with PBS and stained twice with a 4% hydochloric acid and 4% potassium ferrocyanide (1:1 v/v) solution of for 25 minutes (Polysciences Prussian Blue stain kit, PA USA). After washing with PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... High resolution episcopic microscopy (HREM) was performed as previously described97 using an JB-4 embedding kit (00226-1, Polysciences GMBH, Germany). μMRI and HREM data were analysed using OsiriX MD DICOM viewer version 9.0.2 (Pixmeo) ...
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... histological analysis of periodic acid-Schiff stain was performed on mouse kidney sections using PAS Stain Kit (24200-1, Polysciences, Warrington, PA). Images were visualized at 25× magnification using Leica TCS SP8 (Leica Microsystems ...
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bioRxiv - Biophysics 2020Quote: ... and GFP in 1:1:1 ratio using polyethyleneimine (54) (Polysciences, Inc.; 23966-1; lot: 705149). We verified each construct by sequencing before and after subcloning ...
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bioRxiv - Neuroscience 2023Quote: ... at a 1:1:1 ratio using PEI-polyethyleneimine “max,” (Polysciences #24765). Media was changed to serum free DMEM at 16 hours post transfection ...
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bioRxiv - Biophysics 2020Quote: ... mUNC80 and HA-mUNC79 plasmids at a ratio of 1:1:1:1 using polyethylenimine (PEI 25K, Polysciences) and incubated for 24h before recording ...
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bioRxiv - Immunology 2022Quote: ... The transfection was performed with a 1:1 (w:w) HC/LC ratio using a 1:2.5 ratio with 1 mg/l PEImax (Polysciences) in 200 μl Opti-MEM ...
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bioRxiv - Microbiology 2023Quote: ... and pHelper (CellBio Labs) at a 1:1:1 ratio using polyethyleneimine (PolySciences). Supernatant was collected 72hrs post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... and pCAG ATG14 or mCherry-ATG14 plasmids were co-transfected at a 1:1:1:1 mass ratio using the polyethelenimine (PEI) (Polysciences) transfection method ...
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bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...
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bioRxiv - Microbiology 2020Quote: ... Samples were infiltrated with 1:1 Polybed812 (Polysciences) resin:acetone for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... then a 1:1 solution of ethanol:Hexamethyldisiloxazne (HMDS) (Polysciences) followed by pure HMDS ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 mg/mL polyethylenimine (PEI) (Polysciences, 23966-1) in a 4:1 ratio of DNA (μg):PEI (μg) ...
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bioRxiv - Cancer Biology 2021Quote: ... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
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bioRxiv - Bioengineering 2023Quote: ... and pAAV for in a 1:1:1 molar ratio using polyethylenimine (PEI, Cat# 23966, Polysciences). The AAV production media were collected 72 h and 120 h post transfection and concentrated using a PEG-precipitation method with 8% PEG-8000 (wt/vol ...
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bioRxiv - Cell Biology 2023Quote: Transfections were done transiently using PEI transfection reagent (1 mg ml-1 stock, Polysciences, 24765-1), 4 µg of transfection reagent per 1 µg of plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 3:2:1 using 1 mg/ml polyethylenimine (Cat#23966-1, Polysciences, Inc.). Retrovirus was harvested from the medium 48 h post-transfection ...