Labshake search
Citations for Polysciences :
51 - 100 of 702 citations for Rat Heat Shock 60 kDa Protein 1 Chaperonin HSP60 HSPD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: HEK293T cells at 60-70% confluence were transiently transfected with HIV-1 ADA gp145 Env using polyethylenimine (PEI Max, Polysciences) in a 1:3 ratio of plasmid to PEI (weight to weight) ...
-
bioRxiv - Immunology 2020Quote: ... to 100 μL and then added to 6.0 μg of polyethylenimine (PEI) (1 mg/mL, linear, 25 kDa; Polysciences, Warrington, FL, USA) diluted in PBS to 100 μL (3:1 ratio of PEI to DNA (w/w)) ...
-
Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...
-
bioRxiv - Cell Biology 2021Quote: ... 60% confluent HEK293T cells were transfected using PEI (Polysciences, 23966) in OptiMEM (Thermo ...
-
bioRxiv - Immunology 2021Quote: ... Cells were transfected using 60 μg of PEI-Max (Polysciences) with a mix of three plasmids ...
-
bioRxiv - Molecular Biology 2019Quote: ... A solution of PLA (Mw: 80–100 kDa; Polysciences, Warrington, PA, USA) at 10 mg/mL was then dropped onto the PVA-coated substrates and spin-coated at 4,000 rpm for 20 s ...
-
bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were reverse transfected using poly(ethyleneimine) (linear, 20 kDA; Polysciences)49 ...
-
bioRxiv - Cell Biology 2020Quote: ... we used the 40 kDa linear polyethylenimine (Polysciences Europe GmbH, Hirschberg, Germany) at a 1:3 ratio (DNA ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with plasmid DNA and 25 kDa polyethyleneimine (Polysciences, PA) at 1 μg/mL in Freestyle 293 media ...
-
bioRxiv - Cell Biology 2021Quote: ... 293GP2 cells were transfected using linear 25-kDa polyethylenimine (PEI, Polysciences, Inc.), as described previously [34].
-
bioRxiv - Cell Biology 2023Quote: ... Transient transfections were performed using 40-kDa linear polyethyleneimine (PEI MAX, Polysciences) (44) ...
-
bioRxiv - Cell Biology 2023Quote: ... 293T cells were transfected using linear 25-kDa polyethylenimine (PEI, Polysciences, Inc.), as described previously59.
-
bioRxiv - Microbiology 2024Quote: ... HEK 293F cells were transfected using 40 kDa polyethylenamine (PEI-MAX) (Polysciences) at 1 mg ml−1 ...
-
bioRxiv - Microbiology 2024Quote: ... HEK 293F cells were transfected using 40 kDa polyethylenamine (PEI-MAX) (Polysciences) at 1 mg ml−1 ...
-
bioRxiv - Immunology 2022Quote: ... The light and heavy chain expression plasmids of LH01 or anti-PD-L1 were mixed at 2:1 and co-transfected using liner polyethylenimine (PEI) with a molecular weight of 25 kDa (Polysciences, Warrington, PA, USA). LH02 was produced by transfecting HEK293E cells with Fc-Sushi-IL15-expression plasmid alone ...
-
bioRxiv - Cell Biology 2021Quote: ... and embedded at −60°C in Lowicryl HM20 (Polysciences, Warrington, PA). Samples were polymerized at −60°C with and warmed slowly over four days ...
-
bioRxiv - Cell Biology 2023Quote: ... Ultrathin sections (60-80 nm) were stained with uranyl acetate (Polysciences) and lead citrate (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2019Quote: ... Transient transfection was performed with Polyethylenimine (PEI, 25 kDa Polysciences Inc. # 23966-2) mixed with DNA in a 3:1 ratio ...
-
bioRxiv - Genetics 2020Quote: ... with transfection mix composed of 100 ng of 25 kDa linear polyethylenimine (Polysciences) and DNA for GRK2-based Gα activation biosensor (40 ng of FLAG-hGIPR(WT ...
-
bioRxiv - Immunology 2023Quote: ... and 2.2 μg/ml polyethylenimine (linear 25 kDa PEI, Polysciences, Inc, Warrington, PA) to the suspension cultures ...
-
bioRxiv - Systems Biology 2024Quote: ... The seeded cells were transfected using 25 kDa polyethylenimine (PEI, Polysciences Inc., Germany) at a 6:1 ratio of PEI to DNA ...
-
bioRxiv - Neuroscience 2021Quote: Cell transfection was performed with lineal polyethylenimine (MW 25 kDa; Polysciences, Warrington, PA, USA). Parenteral HEK-293T cells (8×106 cells ...
-
bioRxiv - Biochemistry 2020Quote: ... Transfection was carried out using preheated (70°C) 40 kDa polyethyleneimine (PEI-MAX) (Polysciences) at 1mg of plasmid DNA per 1 liter of culture once cell density has reached 1*106 cells/ml ...
-
bioRxiv - Biochemistry 2022Quote: ... Transfection was carried out using preheated (70°C) 40 kDa polyethyleneimine (PEI-MAX) (Polysciences) at 1mg of plasmid DNA per 1 liter of culture once cell density has reached 1*106 cells/ml ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were transfected using polyethylenimine (PEI) 25-kDa linear polymer (Polysciences Corporation Ltd. #23966) or Effectene Transfection reagent (Qiagen #301425 ...
-
bioRxiv - Cell Biology 2023Quote: ... with 100 ng total DNA per well using polyethyleneimine (PEI; linear 25 kDa; Polysciences) at a DNA/PEI mass ratio of 1:3 ...
-
bioRxiv - Bioengineering 2024Quote: ... and delivered to HEK293T cells by transient transfection using linear 25 kDa polyethyleneimine (Polysciences). About 24 h post-transfection ...
-
bioRxiv - Cancer Biology 2023Quote: ... Huh-7 were transfected with linear 25 kDa polyethyleneimine (PEI, Polysciences Inc., Warrington, PA, USA) by using a 3:1 ratio of PEI/DNA (as described in [58]) ...
-
bioRxiv - Biochemistry 2021Quote: ... Lentivirus was produced by transient transfection of HEK293T cells (ATCC) using linear 25 kDa polyethyleneimine (PEI; Polysciences). Briefly ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μg of plasmid DNA was incubated with 100 μg/mL polyethyleneimine (Polysciences Inc; linear 25 kDa) in 500 μL DMEM without serum or antibiotic for 20 minutes at room temperature ...
-
Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... pMD2-VSV-G and pLKO-shRNA or pWPI expressing NS4B using 25 kDa linear polyethylenimine (Polysciences Inc.) exactly as before (Anton et al. ...
-
bioRxiv - Biophysics 2023Quote: ... A ratio of 1.2 µg Plasmid DNA per 106 cells and 40 kDa linear PEI MAX (Polysciences) transfection reagent was used for protein expression ...
-
bioRxiv - Physiology 2023Quote: ... pLNC-ER: RAS vector and packaging vectors using 1mg/mL of linear 25 kDa polyethylenimine (PEI, Polysciences). 24 hours following transfection ...
-
bioRxiv - Immunology 2021Quote: ... The tissues were then washed with 60% isopropanol and mounted in an Aqua Polymount (Polysciences). Tissues were imaged using an Olympus FluoView 3000 confocal microscope (Olympus) ...
-
bioRxiv - Cell Biology 2022Quote: ... 9 µg transfer vector and 2 µg pVSV-G using 60 µl linear PEI (Polysciences). Medium was exchanged 6-8 hours post-transfection and harvested after another 48 hours ...
-
bioRxiv - Biophysics 2021Quote: ... approximately 2.0 mg plasmids (1.4 mg for Nav1.5-E1784K and 0.6 mg for β1) and 4 mg 25-kDa linear polyethylenimines (PEIs) (Polysciences) were mixed into 30 mL fresh medium and pre-incubated for 15-30 min before adding into 1 liter cell culture ...
-
bioRxiv - Cell Biology 2023Quote: ... plasmids were generally transfected at 50-60% of confluency using PEI (polyethyleneimine, Polysciences Inc #23966-2)/ Lipofectamine LTX (Invitrogen #15338100 ...
-
bioRxiv - Molecular Biology 2021Quote: 100 million cells were seeded in 15 cm dishes and the protein-DNA contacts were cross-linked using 1% (v/v) formaldehyde (Polysciences, 04018-1) for 10 min at room temperature with shaking ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Treated rat explants were fixed in 4% PFA (Polysciences, Inc., Warrington, PA, USA) at room temperature for 40 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... lentiviral vector was co-transfected with pSPAX2 and pMD2.G in 293T cells using 40-kDa linearpolyethylenimine (Polysciences, 24,765). Retrovirus generation was by using PLAT-A cells (Cell Biolab) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.25 µg of pcDEF3-hACKR3 and 4.75 µg of empty pcDEF3 DNA were combined with 40 µg of 25 kDa linear polyethylenimine (Polysciences) in a total volume of 500 µL 150 mM NaCl and incubated for 20 minutes at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... and after 4 h the cells were transfected using 60 μg polyethyleimine (PEI, Polysciences, Warrington, PA, USA). Plasmid DNA in an amount of 1.5 μg was diluted in buffer containing 0.15 M NaCl and 20 mM HEPES (pH 7.5 ...
-
bioRxiv - Immunology 2021Quote: ... 1.4 µg of WT-SARS-CoV-2 spike plasmid (2) and 60 µg of PEI-Max (Polysciences). Supernatants were harvested 48h later ...
-
bioRxiv - Immunology 2022Quote: ... The S-2P and S-6P plasmids were transiently transfected into HEK293F cells using 25-kDa linear polyethyleneimine (PEI) (Polysciences) with the PEI:DNA mass ratio of 3:1 and 1 mg DNA for per liter of culture when the cell density reached 2 × 106 cells per mL ...
-
bioRxiv - Biochemistry 2019Quote: ... pcDNA3.1 plasmids encoding for FLAG-CHD4 were transfected into cells using linear 25-kDa polyethylenimine (PEI, Polysciences, Warrington, PA, USA). 50 µg of the DNA mixture was first diluted in 2.7 mL of PBS and vortexed briefly ...
-
bioRxiv - Biochemistry 2020Quote: ... For transfection cells were centrifuged and resuspended in fresh media to a density of 4×106 cells/mL and transfected with 4 μg plasmid/mL and 16 μg/mL of PEI 40 kDa (Polysciences). 4 h up to 24 h after transfection cells were fed with 75% of the transfection volume ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells with a concentration of 106 cells/mL were transfected with 1.5 mg of DNA per liter of the culture using transfection reagent 40 kDa PEI MAX (Polysciences, Inc.) in a 1:2 ratio ...
-
bioRxiv - Bioengineering 2023Quote: ... The light and heavy chain expression plasmids of LH03 or LH05 were mixed with 25 kDa linear polyethylenimine (PEI, Polysciences) and transiently transfected in HEK293E cells ...
-
bioRxiv - Microbiology 2023Quote: The 25kDa PEI 25™ (PEI-25) and 40 kDa PEI Max® (PEI-40) polymers were acquired from Polysciences and were prepared according to manufacturer instructions to get a stock solution of 1 mg/ml ...
-
bioRxiv - Systems Biology 2023Quote: Retrovirus was produced by transiently co-transfecting HEK 293T cells with a plasmid encoding a CAR or control construct and pRD114/pHIT60 virus-packaging plasmids (courtesy of Dr. Steven Feldman of the National Cancer Institute) using linear polyethylenimine (25 kDa; Polysciences). After 48 and 72 hours ...