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Citations for Polysciences :
851 - 900 of 968 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Immunology 2022Quote: ... expi293F cells were transfected with antibody expression plasmids at a density of 2.5 million cells per/ml using 1 mg/ml PEI-Max transfection reagent (Polysciences). Supernatants were harvested 4-5 days post-transfection and tested for their SARS-CoV-2 RBD binding potential by enzyme-linked immunosorbent assay (ELISA) ...
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bioRxiv - Cell Biology 2024Quote: ... or with GFP-tau and vectors pCDNA3 overexpressing Cdk5 and p25 (1:0.85:0.15) using linear Polyethylenimine (PEI; Polysciences, Inc.). Cells were harvested 48 hours after transfection by centrifugation and flash-frozen ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... the remained solvent was blotted and three drops of 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was applied on the grid for staining purpose ...
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bioRxiv - Microbiology 2023Quote: HIV-1 viruses were generated by transfection of proviral DNA into HEK293T cells via polyethylenimine (PEI, Polysciences, Cat. #23966) transfection protocol ...
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bioRxiv - Biophysics 2023Quote: ... The cells in each well were transiently transfected with 1 μg DNA plasmid using 3μg linear polyethyleneimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Imaging was carried out by placing adult worms in 1.5 μl of M9 mixed with 1.5 μl of 1 μm polysterene microspheres (Polysciences Inc.) on a coverslip which was mounted carefully onto a 5% agarose pad.
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bioRxiv - Biochemistry 2023Quote: ... Flp-In Trex cells were co-transfected with a 1:9 ratio (w/w) of GFP vector:pOG44 Flp recombinase vector using PEI Max 40k (Polysciences). To select for integrant cells ...
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bioRxiv - Bioengineering 2023Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed MBV samples were incubated on the grids for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... followed by brief nuclear counterstaining using Hoechst (1:10,000) in PBS and mounting on a glass slide with Aqua-Poly-Mount (Polysciences).
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bioRxiv - Microbiology 2023Quote: ... Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in a sterile aqueous suspension without sodium azide ...
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bioRxiv - Neuroscience 2024Quote: ... the left sides of the brains were placed (anterior portion facing downwards) in disposable cryomold (Polysciences Inc. #18986– 1), and frozen in O.C.T ...
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bioRxiv - Neuroscience 2024Quote: ... the transfer and pDGM6 packaging plasmids were co-transfected in 293T cells (ATCC) using PEI reagent (Polysciences, 23966-1), as described previously 52 ...
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bioRxiv - Microbiology 2024Quote: ... Transient DNA transfections were performed using linear poly(ethylenimine) (1 mg/mL of solution in 10 mM Tris-HCl, pH 6.8; Polysciences), Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... 8.3 µg psPAX and 2.5 µg pMD2.G were mixed with 80 µg PEI MAX 40K (Polysciences, 24765–1) in 1.5 ml of Expi293 media ...
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bioRxiv - Neuroscience 2024Quote: ... Cells at ∼80% confluency were transfected with DNA plasmids using polyethyleneimine max according to the manufacturer’s manual (PEI Max; 24765-1, Polysciences). For CaST experiments ...
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bioRxiv - Bioengineering 2024Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed EV samples were incubated on the grids for 20 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.5-1 μg/mL of each construct was transfected with 5 μg/mL polyethyleneimine (PEI-25K; Polysciences, 23966). 24 hr after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... pEGFP-N1-RHBDL4 was transfected as a 1:10 mix with empty pcDNA3.1 plasmid into Hek293T cells (ATTC) using 25-kDa linear polyethylenimine (Polysciences) as had been described (Knopf et al ...
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bioRxiv - Developmental Biology 2024Quote: ... A second droplet of (∼ 10 µl) of a solution of 1 µm polystyrene microspheres (Polysciences, cat. no. 07310-15) was then added and gently mixed ...
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bioRxiv - Cell Biology 2022Quote: ... six-well plates with about 80-90% confluency were transfected with 1 µg plasmid DNA and 3 µL PEI (Polysciences) per well ...
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bioRxiv - Molecular Biology 2021Quote: Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... expression medium was added to reduce cell density to 1.0 × 106 ml−1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Microbiology 2021Quote: ... the construct was expressed in 293F by transfecting with PEIMAX PEI MAX™ (24765-1, Polysciences, Inc., Warrington, PA, USA) according to the manual ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biophysics 2021Quote: ... were co-transfected with plasmids containing the SOSIP sequence and furin protease in a 3:1 ratio using PEI MAX (Polysciences). Six to seven days post-transfection the supernatant was cleared by filtration and the SOSIP purified by Galanthus nivalis lectin affinity ...
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bioRxiv - Neuroscience 2020Quote: ... The homogenized mixture was then poured into a solution containing 1% polyvinyl alcohol (25 kDa, 88% hydrolys, Polysciences, Warrington, PA) in water under continuous stirring ...
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bioRxiv - Cell Biology 2020Quote: HEK293T stable cell lines for HSPB8-V5 were transiently transfected with different wild type or mutant BAG3-GFP constructs using PEI (23966-1, PolySciences Europe ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 50 to 100 million J-Lat 11.1 cells per condition were collected and crosslinked with 1% formaldehyde (Polysciences inc.) in 40 ml PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were incubated for 2h at RT and then washed in PBS and further incubated for 30 minutes in 1 ml 50 mM ethanolamine (Polysciences). The beads were then washed 3 times in PBS and left in PBS on a cold room rotator.
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... 5 μg of an HIV-1 Gag-Pol expression plasmid (pCRV1/GagPol) and 1 μg of VSV-G expression plasmid in 293T cells in 10-cm dishes using polyethylenimine (PolySciences). Virus-containing supernatant was collected and filtered (0.2μm ...
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bioRxiv - Genetics 2022Quote: Circulating blood monocytes were labeled in vivo by retroorbital intravenous injection of 1 μm Flouresbrite YG microspheres (Polysciences Inc, PA) diluted 1:4 in sterile PBS in mice undergoing regression 3 days prior to harvest ...
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bioRxiv - Immunology 2020Quote: ... the cells were transfected using 6 μg p509 and 6 μg p2670 plasmid DNAs (Neuhierl et al., 2002) mixed with 72 μl of a 1 mg/ml stock solution of polyethylenimine (#24765; Polysciences) in water (pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C for 72 hours after which cells were fixed with 1 ml of 10% formaldehyde (Polysciences) was added for 24 hours to ensure inactivation of virus ...
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bioRxiv - Molecular Biology 2020Quote: ... Yeast strains were grown in rich medium (YPD) to mid-log phase (∼1.5×107/ml) and cross-linked with methanol-free formaldehyde (1% final concentration, Polysciences Inc) for 30 min and quenched with 0.25M final concentration of glycine (from 2.5M stock ...
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bioRxiv - Microbiology 2021Quote: ... were seeded in 24-well plates and transfected the following day using PEI MAX (3:1 PEI to DNA ratio; Polysciences) with 500 ng pHIV-1 and 0-250 ng pcDNA3.1 protein expression construct ...
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bioRxiv - Immunology 2020Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Four hundred μl of undiluted culture was then mixed with 50 μl of a 1:100 dilution of 1.75 μm fluorescent microspheres (Polysciences 19392). Four hundred fifty μl of the bead/culture suspension was added to the epithelial surface of individual tissue sections ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Immunology 2022Quote: The HEK293T cell line was then transfected with both the pYD7-H57Fab-HC-PIF plasmid and the pTT22-H57Fab-LC plasmid (ratio 1:3) using polyethylenimine (PEI, Polysciences). Cells were then maintained in DMEM supplemented with 2% FBS ...
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bioRxiv - Molecular Biology 2024Quote: One 15 cm plate of HEK293T cells was transfected with the indicated plasmids using Opti-MEMTM (31985062, ThermoFischer) and Polyethylenimine (23966-1, Polysciences), followed by harvest after 48 hours ...
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bioRxiv - Biochemistry 2023Quote: ... pCAG-FIP200NTD (1-640) was co-transfected with pCAG-TSF-ATG13363-517-ULK1MIT (836-1050) using the polyethylenimine (PEI) (Polysciences) transfection system ...
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bioRxiv - Immunology 2023Quote: ... NIH)67,70 and a plasmid encoding the full Env gp160 in a 3:1 ratio in HEK293T cells (ATCC) using the PEIMAX transfection reagent (Polysciences). Following 48 hours ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were used for transient transfection with the suitable S2P stabilized S-expression plasmids or other vectors by using 1 mg/mL polyethylenimine (PEI, Polysciences). The supernatant was harvested and the S trimer was purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Each culture well was transfected with 1 μg of FLAG-LRRK1 construct and 500 ng of EGFP-Rab7 using polyethylenimine (Polysciences) and cultured at 37°C with 5% CO2 for 36 h before harvesting.
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bioRxiv - Genetics 2023Quote: ... or recombined forms of eG-SCON-FRT-FP was mixed with 8 μl of polyethyleneimine (1 mg/ml; Polysciences, 23966). These solutions were then incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
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bioRxiv - Immunology 2023Quote: HEK293T cells at 60-70% confluence were transiently transfected with HIV-1 ADA gp145 Env using polyethylenimine (PEI Max, Polysciences) in a 1:3 ratio of plasmid to PEI (weight to weight) ...
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bioRxiv - Microbiology 2024Quote: ... vesicular stomatitis virus G (VSV-G)-pseudotyped HIV-1 viruses were produced by co-transfection of HEK293T cells with an HIV-1 proviral construct and a VSV-G-encoding vector using the PEI reagent (Polysciences). Two days post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... with modified pCW57.1 target vector in a ratio of 5 : 1 : 5 : 5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966). Three days post-transfection ...