Labshake search
Citations for Polysciences :
801 - 850 of 968 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: To identify sensory neurons innervating the knee joint retrograde labeling with Fast Blue (FB, #17740-1, Polysciences) was performed ...
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bioRxiv - Bioengineering 2024Quote: ... 150,000 cells/mL with 1 v/V% 500 nm fluorescent beads (Floresbrite YG carboxylate beads, Polysciences, Inc.) were encapsulated within each hydrogel on a Nunc glass bottom dish (Nunc Glass ...
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bioRxiv - Molecular Biology 2023Quote: ... were purchased from Sigma Aldrich while PSS of Mw = 1 000 (PSS_5) and Mw = 8 890 (Mw/Mn = 1.09) (PSS_43) were bought from Polysciences and Scientific Polymer Products Inc. ...
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bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) or TransIT-293 (Takara ...
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bioRxiv - Biophysics 2024Quote: ... the cells (50% confluency) were transfected with 0.5 µg·well-1 plasmid DNA using polyethyleneimine (PEI Max, Polysciences) and incubated for 16 h at 37 °C in 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... Ten 15-cm culture dishes with 80% confluency were transfected using 6.8 μM polyethyleneimine (Polysciences, #24765-1), 70 µg AAV transgene plasmid (pAAV-EF1a-GCaMP6s-WPRE-pGHpA ...
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bioRxiv - Synthetic Biology 2024Quote: Transient transfection was performed using polyethylenimine (PEI) (Polysciences, 23966, prepared as a .323 g l-1 stock). For each transfected well of a 24-well plate ...
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bioRxiv - Biophysics 2024Quote: ... A total of 10 mL (1 mg/mL in PBS) of PEI Max 40 kDa (Polysciences Inc.) was added to the DNA and incubated for 20 min at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... 100 µg of plasmid DNA was incubated with 300 µg of polyethylenimine (PEI 25,000) (Polysciences, 23966-1) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293FT cells were transfected with the lentiviral vector pCLTs together with the packaging plasmids pCMV-VSVG-RSV-Rev (addgene) and pCAG-HIVgp (addgene) at a 1:0.5:0.5 ratio using PEI Max reagent (Polysciences) according to the Manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: For sectioning embryos in 100% methanol were mounted in JB4 plastic sectioning medium (Polysciences cat. no 00226-1). Dehydrated samples were infiltrated with 10 ml JB4 solution A plus catalyst for two days ...
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bioRxiv - Biophysics 2021Quote: ... cells were transfected using Env-deficient HIV-1 backbone plasmid pSG3ΔEnv (NIH ARRRP) and 25 kDa PEI (Polysciences) as a transfection reagent ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with 67.5 µg of polyethylenimine (PEI) Max 40,000 (Polysciences, Inc, Cat. No. 24765-1) in complex with 3.15 µg of plasmid encoding a spike variant ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were transiently transfected with 2mg expression plasmid per 1 liter using the Polyethylenimine (PEI) MAX (Polysciences) at a density of 1.0 x 106 cells/ml in fresh Union 293 medium ...
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bioRxiv - Microbiology 2021Quote: ... Samples were washed in sodium cacodylate buffer at room temperature and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h ...
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bioRxiv - Biophysics 2021Quote: ... For traction force microscope gels were manufactured as above by additioning 0.2 μm fluorescent beads (1:125; Polysciences). Gels were washed in 70% Ethanol and rinsed extensively in PBS after which transfected cells were plated ...
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bioRxiv - Immunology 2020Quote: ... pEE12.4-RBD-SpyTag-Histag plasmids were transfected with PEI (PEI MAX-Transfection Grade LinearPolyethylenimine Hydrochloride, Polysciences 24765-1) into 293F cells ...
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bioRxiv - Neuroscience 2021Quote: ... worms were fed a 50% dilution of a 2.5% weight by volume (4.55 x 1010 particles per mL) solution of 1 µm diameter polystyrene beads (Polysciences, Inc ...
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bioRxiv - Biophysics 2021Quote: ... Transfection solution was prepared by combining 20 μL (per dish, hereafter) of polyethylenimine solution (Polysciences; 1 mg/mL) and a plasmid mixture consisting of 500 ng Lg-βarr1 or Lg-βarr2 and 1μg μOR-Sm in 1 mL of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... GST-ATG9 (828-839)-ATG101 were co-transfected with ATG13 (1-197) using the polyethylenimine (PEI)-MAX (Polysciences) transfection system ...
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bioRxiv - Cancer Biology 2022Quote: ... and 12 μg of pLKO-shRNA-puro using 63 μL of polyethylenimine (PEI – 1 μg/μL; Polysciences - 24765) mixed in 750 μL of 10 mM HEPES pH 7.4 and 150 mM NaCl in water ...
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bioRxiv - Physiology 2022Quote: ... 16 μg AAV-U6-sgRNA-TBG-Cre plasmid and 144 μl of 1 mg/ml Polyetherimide (PEI) (Polysciences) was mixed in 9 ml OptiMEM (Thermo) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were transfected using a polyethylenimine (PEI) reagent (2.5 µl of 1 mg/ml per well hereafter; Polysciences) with these UTS2R plasmids (100 ng per well hereafter) ...
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bioRxiv - Biophysics 2024Quote: ... Cells were transfected using a polyethylenimine (PEI) agent (10 μl of 1 mg/ml per dish hereafter; Polysciences) with pCAG plasmids encoding human A1R ...
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bioRxiv - Microbiology 2023Quote: ... Samples were washed in sodium cacodylate buffer at room temperature and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with 67.5 μg of polyethyleneimine (PEI) Max 40,000 (Polysciences, Inc, Cat. No. 24765-1) in complex with 3.15 μg of plasmid encoding a spike variant ...
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bioRxiv - Biophysics 2023Quote: ... Negative staining was performed using 1% uranyl acetate (98% uranyl acetate; ACS Reagent, Polysciences Inc. Warrington, PA, USA) solution for 20 s ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were transfected with 121.6 μL of 1 mg/mL of linear Polyethylenimine (PEI; MW 25,000; Polysciences) and 30.4 μg of plasmid DNA diluted in Opti-MEM (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... 1.2 μg of DNA was mixed with 2.4 μL of 1 mg/mL polyethylenimine (linear PEI, MW 25,000, Polysciences) in one tenth of the final culture volume of medium ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293T cells were cultured in T25 flasks and transfected at ∼70% confluency with 2.5 μg of the pCDH lentiviral transfer vector of interest and packaging plasmids psPAX2 (1.25 μg) and pMD2.g (1.25 μg) with 25 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... reference beads (1 μm Fluoresbrite® BB Carboxylate Microspheres; Ref. 17458, Lot. 659681; Polysciences, Inc., Warrington, PA, USA) were added as an internal sample standard ...
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bioRxiv - Neuroscience 2024Quote: ... young adult animals were mounted onto 1-1.5% of agar pad with 3-5 µL of latex beads (Polybeads, 0.2 µm, PolySciences) to facilitate body movement and stabilize the position of field of view22 ...
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bioRxiv - Neuroscience 2024Quote: ... 5.7μg of construct DNA was transfected with 22.8μg of capsid plasmid and 11.45μg of pADDeltaF6 using polyethylenimine 25K MW (Polysciences, 23966-1). Collection of cells and media for AAV harvesting began 72 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... into one 15-cm plate of confluent HEK-293T cells using PEI Max (Polysciences Inc., catalog # 24765-1). At one day post-transfection we changed the medium to low serum (1% FBS) ...
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bioRxiv - Biophysics 2024Quote: ... Negative staining was performed using 1% uranyl acetate (98% uranyl acetate; ACS Reagent, Polysciences Inc. Warrington, PA, USA) solution for 20 s ...
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Numb provides a fail-safe mechanism for intestinal stem cell self-renewal in adult Drosophila midgutbioRxiv - Developmental Biology 2024Quote: ... The entire gastrointestinal tract was taken and fixed in 1 X PBS plus 8% EM grade formaldehyde (Polysciences) for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... The fixed suspension of bacteria was washed in sodium cacodylate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h ...
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Novel amiloride derivatives that inhibit bacterial motility across multiple strains and stator typesbioRxiv - Microbiology 2021Quote: ... and incubated for 10 mins and washed with motility buffer and flushed with 1 μm polystyrene bead (Polysciences, Inc) solution ...
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bioRxiv - Immunology 2021Quote: ... 480µg of each plasmid was co-transfected with 120µg of BirA (Howarth et al., 2008) and 12mg PEI-Max (1 mg/mL solution, Polysciences) in the presence of 200 µM biotin (final concentration) ...
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bioRxiv - Neuroscience 2021Quote: ... Day 1 adults were placed on a 10% agarose pad and immobilized with a slurry of polystyrene beads (Polysciences). A 1KHz train of 100 fs pulses (15-30 nJ/pulse ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were cultured in T75 flasks and transfected at approximately 70% confluency with 7500 ng of the lentiviral vector of interest and packaging plasmids pCMV-dR8.91 (6750 ng) and pCMV-VSV-G (750 ng) with 75 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... pSIREN- or pMXs-based plasmids were co-transfected together with pVSVG into Plat-E cells (kindly provided by T. Kitamura, University of Tokyo) using PEI Max (Mw 40,000, 24765-1; Polysciences). The virus-containing supernatant was harvested 2 days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... and sorting efficiency was verified manually by sorting a specified number of 1-μm yellow-green microspheres (Polysciences, USA) onto a glass slide and counting the beads under an epifluorescence microscope ...
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bioRxiv - Microbiology 2022Quote: ... B16F10 cells (2,500,000 cells) were transfected with 5 μg S expression plasmid by PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The cells at 1.5*106 confluence were transiently transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated at 37°C with 8% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... was performed by transfecting HEK293T cells in five 15 cm tissue culture plates using PEI (Polyethylenimin Linear, MW 25,000, 23966-1-A, Polysciences) transfection method (45) ...
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bioRxiv - Biochemistry 2020Quote: ... For formation of DNA:PEI complexes 1 μg DNA/mL transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
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Fast and sensitive diffuse correlation spectroscopy with highly parallelized single photon detectionbioRxiv - Neuroscience 2020Quote: Our decorrelating tissue phantom was created by a 1 μm polystyrene microsphere-in-water suspension (Polysciences, inc.; Warrington, PA) in custom made cuvettes ...