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Citations for Polysciences :
651 - 688 of 688 citations for Triclosan 13C12 99% 100 Ug Ml In Mtbe 2 4 4 Trichloro 2 Hydroxydiphenyl Ether since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The transfection solution was prepared by combining 25 µl (per dish hereafter) of polyethylenimine (PEI) Max solution (1 mg ml-1; Polysciences), 1 ml of Opti- MEM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... These vectors were transiently transfected into 293F cells at a concentration of 1 million/ml with 1 μg of DNA per million cells using branched polyethylenimine (PEI) (Polysciences). 60-72 hours post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were used for transient transfection with the suitable S2P stabilized S-expression plasmids or other vectors by using 1 mg/mL polyethylenimine (PEI, Polysciences). The supernatant was harvested and the S trimer was purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... or recombined forms of eG-SCON-FRT-FP was mixed with 8 μl of polyethyleneimine (1 mg/ml; Polysciences, 23966). These solutions were then incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... freshly seeded 293T cells grown in DMEM supplemented with 10% FBS + 50 µg/mL gentamycin at 37°C with 5% CO2 (hereafter, supplemented DMEM) were transfected using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection was performed when the cell density reached 2.5 × 106 cells per mL by using expression plasmids and polyethylenimines (PEIs, Polysciences, USA). Approximately 1.5 mg of plasmids were premixed with 4.5 mg of PEIs in 50 mL of fresh medium for 25 minutes before application ...
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bioRxiv - Neuroscience 2023Quote: ... pUCmini-iCAP-PHP.eB and pHelper plasmid with the plasmid ratio of 1:4:2 based on micrograms of DNA were transfected into cells at >90% confluency with 1mg/ml polyethyleneimine (PEI, Linear, MW 25000, Polysciences). pUCmini-iCAP-PHP.eB for PHP.eB production was a gift from Dr ...
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bioRxiv - Genetics 2021Quote: ... -eG-DECAI-FP or recombined forms of eG-SCON-FP or eG-DECAI-FP) was mixed with 8 μl of polyethyleneimine (1 mg/ ml; Polysciences, 23966) and incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by incubation on a mixer at room temperature overnight in an approximately 1 mL mixture of 1 part propylene oxide and 1 part Polybed resin solution (Poly/Bed® 812 Embedding Media, Polysciences, Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Immunology 2022Quote: ... 6 µg pMSCV retroviral plasmid and 3.5 µg pCL-Eco packaging vector were preincubated in 500 µl of complete DMEM at room temperature for 15 min before mixing with 500 µl of 80 µg/ml PEI max (Polysciences, 24765) in complete DMEM ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... were transiently transfected with a mixture of plasmid DNA diluted in PBS (0.67 μg total plasmid DNA per ml of culture) and Polyethylenimine (PEI) (Polysciences, Inc., 23966) at a DNA-to-PEI ratio of 1:3 ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Microbiology 2021Quote: ... HEK293F cells grown in 10 ml of medium (3.0 x 106 cells/ml) were transfected with 6 µg of pCAGGS-NP (1-395) using Polyethyleneimine MAX (Polysciences, Warrington, PA, USA). Three days post-transfection ...
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bioRxiv - Immunology 2021Quote: ... The plasmids of 9μg pHAGE-luciferase-GFP、 psPAX2 and Zhou-COVID-19-Spike were co-transfected into HEK293T cells by using 1 μg/mL polyetherimide (Polysciences, Warrington, PA) in DMEM medium containing 10% FCS ...
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bioRxiv - Biophysics 2022Quote: ... 100-200 µg of purified bacmid in 1 mL water were incubated with 400 µg of 25000 MW polyethyleneimine (PEI; Polysciences Cat# 23966) in 1 mL water at 55 degC for 30-45 minutes to sterilize ...
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bioRxiv - Immunology 2023Quote: ... at a density of 2.0×106 cells/ml and co-transfected with pMlink-N-FLAG-p22phox-NOX2 and pMlink-EROS using PEI 4000 reagent (Polysciences, Warrington, PA). The transfected cells were cultured for 48 h and collected by centrifuged (4,000 × g ...
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bioRxiv - Physiology 2024Quote: ... The balloon was inflated after the first diagonal branch of the LAD in each animal and 3 ml polystyrene microspheres (Polybead, 90 μm, Polysciences, Warrington, PA) were slowly injected via the lumen of the angioplasty catheter ...
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bioRxiv - Neuroscience 2023Quote: ... amphotericin B (0.5 mg ml−1) and small amounts of glass beads (5–15 μm in diameter; Polysciences, Inc., Warminster, PA, USA) (pH 7.3 ...
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bioRxiv - Immunology 2022Quote: ... were diluted with 150 mM NaCl to a concentration of 40 μg/mL and mixed with 40-kDa polyethyleneimine (PEI, Polysciences, Warrington, PA, USA) (DNA ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with the transfection solution that was prepared by combining 8 µL (per dish, hereafter) polyethyleneimine (PEI) solution (1 mg/mL; Polysciences, Warrington, PA, USA) and a plasmid mixture consisting of 400 ng human LPAR-encoding plasmids ...
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bioRxiv - Molecular Biology 2022Quote: ... and 0.625 µg of pMD2.G) using 15 µL of 1 mg/mL transfection grade linear polyethylenimine hydrochloride (MW 40,000) (PEI-MAX-40K) (Polysciences, Warrington, PA, United States) in 500 µL of Opti-MEM ...
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bioRxiv - Synthetic Biology 2024Quote: ... 300 frames) of intravenously perfused (15 µl/min) 1 µm large fluorescent microspheres (Yellow/Green Fluoresbrite carboxylate, Polysciences, 107 microspheres/ml 1x PBS). Speed was determined from the centerline microsphere ...
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bioRxiv - Cancer Biology 2022Quote: ... These vectors along with lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK293FT cells by polyelthylenimine (Polysciences, Inc; 60 μg/mL) mediated transfection ...
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bioRxiv - Neuroscience 2023Quote: ... Transfection with the plasmid expressing PH-PLCδ::GFP probe was done 24 hours before the imaging experiments using Polyethylenimine solution (PEI; Polysciences Inc.; 1 mg/ml) when the cells were around 70% confluent.
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bioRxiv - Cancer Biology 2024Quote: ... Warm cross-link reversal buffer (200 µl 800 mM Tris-HCl pH8.0) was added followed by addition of 6 µl of 1:10 Biomag amine paramagnetic beads (48 mg/ ml, Polysciences cat. no. 86001-10). Homogenization was repeated ...
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bioRxiv - Pathology 2022Quote: The blood flow during vascular colonization was monitored by perfusing 1 μm diameter fluorescent microspheres (Yellow/Green Fluoresbrite carboxylate, Polysciences, 107 microspheres/ml 1x PBS) at a rate of 15 μl/min before each z-stack series and images acquired on a single plane at high speed (50 frames per second ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 ×106 cells/mL using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 48 h ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected at a cell density of 2.5×106 cells/mL by co-transfecting plasmids (1.1 mg total DNA/L cells) using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells).
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bioRxiv - Evolutionary Biology 2023Quote: 1,5×105 Cells were seeded on 15 mm round glass coverslips coated with polyethylenimine (PEI) solution (25 g/ml PEI, Polysciences Inc. and 150 nM NaCl) for 48 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Cell Biology 2019Quote: ... 500 ng DNA (per well of a 12-well plate) or 1500 ng DNA (per well of a 6-well plate) using polyethylenimine (PEI, Polysciences Inc., dissolved at 1 mg/ml in ethanol). Transfection mixtures were made in Optimem using a 1:3 ratio (μg:μl ...