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Citations for Polysciences :
651 - 700 of 727 citations for Triclosan 13C12 99% 100 Ug Ml In Mtbe 2 4 4 Trichloro 2 Hydroxydiphenyl Ether since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were transfected using a polyethylenimine (PEI) reagent (2.5 µl of 1 mg/ml per well hereafter; Polysciences) with these UTS2R plasmids (100 ng per well hereafter) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5 µg of transfer plasmid were transfected with polyethylenimine (8mg/mL sterlile water, Polysciences, Warrington, PA, USA). After 60–72 h of incubation ...
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bioRxiv - Biophysics 2024Quote: ... Cells were transfected using a polyethylenimine (PEI) agent (10 μl of 1 mg/ml per dish hereafter; Polysciences) with pCAG plasmids encoding human A1R ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were transfected with 121.6 μL of 1 mg/mL of linear Polyethylenimine (PEI; MW 25,000; Polysciences) and 30.4 μg of plasmid DNA diluted in Opti-MEM (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... 1.2 μg of DNA was mixed with 2.4 μL of 1 mg/mL polyethylenimine (linear PEI, MW 25,000, Polysciences) in one tenth of the final culture volume of medium ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293T cells were cultured in T25 flasks and transfected at ∼70% confluency with 2.5 μg of the pCDH lentiviral transfer vector of interest and packaging plasmids psPAX2 (1.25 μg) and pMD2.g (1.25 μg) with 25 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours post-transfection ...
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bioRxiv - Immunology 2021Quote: ... 480µg of each plasmid was co-transfected with 120µg of BirA (Howarth et al., 2008) and 12mg PEI-Max (1 mg/mL solution, Polysciences) in the presence of 200 µM biotin (final concentration) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were cultured in T75 flasks and transfected at approximately 70% confluency with 7500 ng of the lentiviral vector of interest and packaging plasmids pCMV-dR8.91 (6750 ng) and pCMV-VSV-G (750 ng) with 75 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours after transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T cells in a 10 cm dish were transfected with 40 µL 1.0 mg/mL polyethylenimine (Polysciences, Warrington, PA) and 15 µg plasmid and incubated for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... the pLHCX or pQXCIP packaging vector of interest and pCMV VSV-G using 1mg/ml PEI®-MAX (Polysciences). Media was replaced 16 h post-transfection and viral supernatants were harvested through a 0.45 µm filter ...
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bioRxiv - Biochemistry 2020Quote: ... For formation of DNA:PEI complexes 1 μg DNA/mL transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
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bioRxiv - Biophysics 2022Quote: ... with the density of about 2.5 million cell/ml were transfected using 3 mg plasmid by poly-ethylenimine (PEI, Polysciences) with a 1:3 plasmid to PEI weight ratio ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Bioengineering 2024Quote: ... Twenty-four hours after transfection with 0.5 μg of plasmid DNA per well using 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.), cells were incubated in HBSS containing 10 mM HEPES-NaOH (pH 7.4 ...
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bioRxiv - Bioengineering 2024Quote: ... transfection was carried out using 0.5 μg of plasmid DNA and 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.). Twenty-four hours after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... cells were transfected with 0.5 μg of plasmid DNA per well using 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.). Twenty-four hours after transfection ...
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bioRxiv - Microbiology 2024Quote: ... Transient DNA transfections were performed using linear poly(ethylenimine) (1 mg/mL of solution in 10 mM Tris-HCl, pH 6.8; Polysciences), Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... were transfected with the plasmid DNA diluted in PBS (0.67 μg total plasmid DNA per ml of culture) using polyethylenimine (Polysciences, Inc.) at a DNA-to-PEI ratio of 1:3 ...
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bioRxiv - Cell Biology 2020Quote: ... and 15µg of an AAV plasmid carrying the transgene of interest were mixed in OptiMEM with PEI-MAX (final concentration 80µg/ml, Polysciences #24765). 2ml of this solution were then added dropwise to each of the 6 HEK293T 15cm2 plates ...
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bioRxiv - Biochemistry 2019Quote: ... followed by ∼3 microliters of the final assay solution containing 5 ng mL-1 500 nm diameter beads (PolySciences Inc,), coated nonspecifically with neutravidin18(ThermoFisher Inc.).
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bioRxiv - Microbiology 2021Quote: ... expression medium was added to reduce cell density to 1.0 × 106 ml−1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Neuroscience 2020Quote: ... and light chains (CA5-VL(X3)_pHL_sec) at a 1:1 ratio using polyethyleneimine (PEI, 1 mg/ml, MW 40,000, Polysciences Cat.# 24765) transfection reagent ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Bioengineering 2020Quote: ... was applied at 1 μg/mL for 90 minutes to stain the nuclei and the samples were washed and mounted with AquaPoly/Mount (Polysciences). Whole and pseudo-islets were imaged on a Zeiss NLO LSM 710 laser scanning confocal microscope (Carl Zeiss).
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bioRxiv - Cell Biology 2022Quote: ... Beads were incubated for 2h at RT and then washed in PBS and further incubated for 30 minutes in 1 ml 50 mM ethanolamine (Polysciences). The beads were then washed 3 times in PBS and left in PBS on a cold room rotator.
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bioRxiv - Microbiology 2020Quote: ... Infections were performed by spinoculation for 2 hours at 1400 x g with VSVg pseudotyped HIV-1 at MOI of 5 in the presence of 1 μg/ml PEI (Polysciences) and 16 μg/ml Polybrene (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ... RIKEN BRC) [22] were mixed with 111 µl of 1 mg/ml PEI MAX® (pH7.5) (Cat. No. 24765-1, Polysciences) in Opti-MEM™ I Reduced Serum Medium (Cat ...
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bioRxiv - Microbiology 2019Quote: ... expression medium was added to reduce cell density to 1.0×106 ml-1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Immunology 2020Quote: ... the cells were transfected using 6 μg p509 and 6 μg p2670 plasmid DNAs (Neuhierl et al., 2002) mixed with 72 μl of a 1 mg/ml stock solution of polyethylenimine (#24765; Polysciences) in water (pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C for 72 hours after which cells were fixed with 1 ml of 10% formaldehyde (Polysciences) was added for 24 hours to ensure inactivation of virus ...
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bioRxiv - Molecular Biology 2020Quote: ... Yeast strains were grown in rich medium (YPD) to mid-log phase (∼1.5×107/ml) and cross-linked with methanol-free formaldehyde (1% final concentration, Polysciences Inc) for 30 min and quenched with 0.25M final concentration of glycine (from 2.5M stock ...
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bioRxiv - Immunology 2020Quote: Cell numbers per tissue were determined by mixing 20 µl of each single cell suspension into a 96-well plate with 50 μl of 8.4 × 104 Fluoresbrite Carboxylate YG 10 µm microspheres/ml (Polysciences Inc.) and 180 μl staining media (dPBS + 2% FBS ...
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bioRxiv - Microbiology 2020Quote: ... at a density of 2.5 × 106 cells/ml were transfected with microbody expression vector plasmid DNA using polyethyleneimine (Polysciences, Inc) at a 1:3 plasmid:PEI ratio ...
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bioRxiv - Immunology 2021Quote: ... HEK293F were grown to a density of ∼1.0 × 106 cells/ml and were co-transfected with heavy chain (HC) and (LC) plasmids (1:1) mixed with PEIMAX (Polysciences). After five days ...
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bioRxiv - Biochemistry 2022Quote: ... The transfection solution was prepared by combining 25 µl (per dish hereafter) of polyethylenimine (PEI) Max solution (1 mg ml-1; Polysciences), 1 ml of Opti- MEM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... These vectors were transiently transfected into 293F cells at a concentration of 1 million/ml with 1 μg of DNA per million cells using branched polyethylenimine (PEI) (Polysciences). 60-72 hours post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... pUCmini-iCAP-PHP.eB and pHelper plasmid with the plasmid ratio of 1:4:2 based on micrograms of DNA were transfected into cells at >90% confluency with 1mg/ml polyethyleneimine (PEI, Linear, MW 25000, Polysciences). pUCmini-iCAP-PHP.eB for PHP.eB production was a gift from Dr ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were used for transient transfection with the suitable S2P stabilized S-expression plasmids or other vectors by using 1 mg/mL polyethylenimine (PEI, Polysciences). The supernatant was harvested and the S trimer was purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... or recombined forms of eG-SCON-FRT-FP was mixed with 8 μl of polyethyleneimine (1 mg/ml; Polysciences, 23966). These solutions were then incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... freshly seeded 293T cells grown in DMEM supplemented with 10% FBS + 50 µg/mL gentamycin at 37°C with 5% CO2 (hereafter, supplemented DMEM) were transfected using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection was performed when the cell density reached 2.5 × 106 cells per mL by using expression plasmids and polyethylenimines (PEIs, Polysciences, USA). Approximately 1.5 mg of plasmids were premixed with 4.5 mg of PEIs in 50 mL of fresh medium for 25 minutes before application ...
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bioRxiv - Cell Biology 2024Quote: ... and 800 ng of the desired pLenti CMV Puro DEST or pLenti-TRE-rtta3G-BLAST construct using 7 μl of 1mg/ml polyethylenimine (PEI) (Polysciences). Approximately 48 hours post transfection ...
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bioRxiv - Biophysics 2024Quote: ... Transfection solution was prepared by combining 6 µL (per dish hereafter) of polyethylenimine (PEI) Max solution (1 mg ml-1; Polysciences), 200 µL of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... -eG-DECAI-FP or recombined forms of eG-SCON-FP or eG-DECAI-FP) was mixed with 8 μl of polyethyleneimine (1 mg/ ml; Polysciences, 23966) and incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by incubation on a mixer at room temperature overnight in an approximately 1 mL mixture of 1 part propylene oxide and 1 part Polybed resin solution (Poly/Bed® 812 Embedding Media, Polysciences, Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...