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Citations for Polysciences :
651 - 700 of 789 citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and after 4 h the cells were transfected using 60 μg polyethyleimine (PEI, Polysciences, Warrington, PA, USA). Plasmid DNA in an amount of 1.5 μg was diluted in buffer containing 0.15 M NaCl and 20 mM HEPES (pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Segments of 0.5cm from flexible branches were fixed with 4% w/v acrolein (Polysciences, Warrington, PA, USA) in a modified piperazine-N ...
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bioRxiv - Cell Biology 2020Quote: ... 5*10^6 293FT cells were transfected in 10 cm dishes using 4 μg polyethylenimine (PEI, Polysciences) per μg plasmid DNA with lentiCRISPR_V2 (Addgene 52961) ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed 4 x 5 min in PBS and mounted with Aqua-Poly/mount (Polysciences, 18606). STED images were acquired by sequential acquisition of each channel ...
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bioRxiv - Neuroscience 2023Quote: ... 4 µL FastBlue (FB; 2% in sterile PBS; CAS# 73819-41-7; Polysciences Inc., Warrington, PA USA) per paw was then slowly injected ...
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bioRxiv - Developmental Biology 2024Quote: ... Testes or cauda epididymis were fixed at 4°C in Bouin’s solution (Polysciences, Inc., Warrington, PA, USA) and were processed for paraffin embedding ...
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bioRxiv - Microbiology 2020Quote: ... A 1 mL sample of exponential phase bacteria was pelleted at 6,000 rcf and resuspended in 1 mL fix (2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid transfections were performed with 1 μg of DNA per single well of a 6-well plate using PEI MAX (Polysciences, INC.) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... -eG-DECAI-FP or recombined forms of eG-SCON-FP or eG-DECAI-FP) was mixed with 8 μl of polyethyleneimine (1 mg/ ml; Polysciences, 23966) and incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biophysics 2021Quote: 1×107 cells were seeded in 75cm2 flasks 24 h prior to transfection with Polyethylenimine (PEI 25K, Polysciences Europe GmbH, Germany) at a DNA to PEI ratio (w/w ...
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bioRxiv - Microbiology 2022Quote: ... and 100 ng of the G2-tagged targets (CTD, RPB1 or RPB2, respectively) using polyethyleneimine (PEI-max, #24765-1 Polysciences Inc). When indicated ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection reactions were prepared by the addition of 2-10 ug of bacmid DNA to 100 μl 1xPBS and 12 μL polyethyleneimine (PEI) at 1 mg/mL (Polyethyleneimine “Max” MW 40.000, Polysciences #24765, USA) to 100 μL 1xPBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were washed 3x 20 minutes in 1% Triton X-100 PBS at room temperature and tissues preserved under coverslips using Aqua-Poly/Mount (Polysciences, 18606). The following primary and secondary antibodies at indicated concentrations were used ...
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bioRxiv - Microbiology 2021Quote: ... Optimization and calibration of the quantification parameters were achieved using yellow-green 1 μm microspheres (441/485 ex/em; Polysciences, PA). Live cyanobacteria were also quantified using Syto9 using FITC filters ...
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bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... and transient transfection was achieved after 48h when reaching 80% confluency in a 1:3 ratio DNA (μg): PEI (μL) (Polysciences, USA) at 1mg/mL in molecular grade water ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection reactions were prepared by the addition of 2-10 μg of bacmid DNA to 100 μL 1xPBS and 12 μL polyethyleneimine (PEI) at 1 mg/mL (Polyethyleneimine ‘‘Max’’ MW 40.000, Polysciences #24765, USA) to 100 μL 1xPBS ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293™ cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Bioengineering 2022Quote: ... and gRNA expression plasmid (6 μg) were co-transfected with transfection reagent at a 2.6/1 ratio of Polyethylenimine (PEI; Polysciences, Warrington, USA) (μg)/total DNA (μg) ...
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bioRxiv - Microbiology 2022Quote: ... mice were fasted for two hours and then given a single dose of 1×106 10 μm Fluoresbrite® YG carboxylate microspheres (Polysciences) via oral gavage ...
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Neuroscience 2022Quote: ... cell nuclei were stained by DAPI for 1 min at RT and tissues were mounted in Aqua-Poly/Mount (Polysciences, #18606). Images were acquired using Zeiss LSM 880 confocal microscope with a Zeiss 20x/0.8 Plan-Apochromat objective and later analyzed using ImageJ.
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bioRxiv - Bioengineering 2024Quote: ... negative staining was performed by depositing for 10 minutes with the gird membrane side down on a drop of 1% (w/v) uranyl acetate (Polysciences, USA). The remaining solution on the grid was removed using filter paper (Whatman ...
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bioRxiv - Molecular Biology 2024Quote: ... was co-transfected with a pcDNA5/FRT/TO plasmid containing the gene of interest in a 1:10 ratio using polyethylenimine “MAX” (Polysciences, 24765). After medium exchange 6 h post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with 100 μg plasmid in complex with 250 μg PEI MAX 4000 (Polysciences, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... 5.7 μg/150 mm dish of construct DNA was transfected with 22.8 μg/150 mm dish of pAAV9 capsid plasmid and 11.45 μg/150 mm dish of pAdDeltaF6 helper plasmid using polyethylenimine 25K MW (Polysciences, 23966-1). Collection of cells and media for AAV harvesting began 72 hours following transfection before iodixanol gradient ultracentrifugation purification using a Type 70 Ti Fixed-Angle Titanium Rotor (Beckman-Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... DAPI was added to the PBS at 1:2500 for nuclear staining) and then mounted on charged glass-slides and cover-slipped in Aqua Poly/Mount (Polysciences, Inc.). Primary antibodies used ...
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bioRxiv - Neuroscience 2024Quote: ... AAV vectors were generated through a transient transfection method utilizing three plasmids and linear polyethylenimine (Polysciences, Warrington, PA, USA, 24765-1) for the process ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293 and HEK293 β-arrestin1/2 KO cells were transfected in suspension with a total of 1 μg DNA and 6 μg 25 kDa linear polyethyleimine (PEI, Polysciences Inc.) in 150 mM NaCl solution per 1 million cells ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293 cells were transfected with a total of 2 µg DNA per 1 x 106 cells using 6 µg polyethyleneimine (PEI, Polysciences Inc.) as a transfection reagent in 150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected at 60-70% confluence using polyethyleneimine (PEI) as the transfection reagent from Polysciences (cat. no. #24765-1).The cell medium was replaced with conditioned culture medium 5 hours post-transfection ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were transfected with a total of 1 μg DNA and 6 μg 25 kDa linear polyethyleimine (PEI, Polysciences Inc.) in 150 mM NaCl solution per 1 × 106 cells ...
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bioRxiv - Microbiology 2021Quote: ... HEK293F cells grown in 10 ml of medium (3.0 x 106 cells/ml) were transfected with 6 µg of pCAGGS-NP (1-395) using Polyethyleneimine MAX (Polysciences, Warrington, PA, USA). Three days post-transfection ...
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bioRxiv - Bioengineering 2021Quote: ... DexMA was dissolved at 0.5 g mL-1 in a 1:1 mixture of milli-Q water and dimethylformamide with 1% (w/v) Irgacure 2959 photocrosslinker and 0.625 mM methacrylated rhodamine (Polysciences, Inc., Warrington, PA). For matrix displacement studies ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with 100 µg plasmid in complex with 250 µg PEI MAX 4000 (Polysciences, Inc, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post transfection ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then rinsed 1 x 5 min with Tris buffer without milk powder and mounted using Aqua-Poly/Mount (Polysciences, Eppelheim, Germany). Antibodies were specific for peripheral myelin protein (PMP2 ...
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bioRxiv - Immunology 2022Quote: ... the next day cells were first transfected with 1 μg/well of the paT7 plasmids using polyethyleneimine (PEI) (Polysciences, Inc. Warrington, PA); 2 hours later ...
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bioRxiv - Cancer Biology 2022Quote: ... or HEK293T cells were seeded for 24h and transiently transfected with the indicated vectors using either polyethyleneimine polymer (Polysciences Inc; 23966-1) TransIT-X2 Dynamic Delivery System (Mirus Bio ...
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bioRxiv - Cell Biology 2021Quote: ... 293T cells grown to 70-80% confluency were transfected with 6 μg ITR-containing plasmid and 22 μg pDGM6 using 112 μg acidified PEI (Polysciences, 23966-1). 48-72 hours after transfection ...
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... histological analysis of periodic acid-Schiff stain was performed on mouse kidney sections using PAS Stain Kit (24200-1, Polysciences, Warrington, PA). Images were visualized at 25× magnification using Leica TCS SP8 (Leica Microsystems ...
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bioRxiv - Microbiology 2022Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences, cat#24765). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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bioRxiv - Immunology 2020Quote: ... to 100 μL and then added to 6.0 μg of polyethylenimine (PEI) (1 mg/mL, linear, 25 kDa; Polysciences, Warrington, FL, USA) diluted in PBS to 100 μL (3:1 ratio of PEI to DNA (w/w)) ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...