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Citations for Polysciences :
551 - 600 of 789 citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Numb provides a fail-safe mechanism for intestinal stem cell self-renewal in adult Drosophila midgutbioRxiv - Developmental Biology 2024Quote: ... The entire gastrointestinal tract was taken and fixed in 1 X PBS plus 8% EM grade formaldehyde (Polysciences) for 2 hours ...
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bioRxiv - Immunology 2021Quote: Flies were dissected in ice-cold PBS and fixed with 4% PFA in PBS (Polysciences) for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were rinsed before being cover-slipped with mounting media (Mowiol 4-88, Polysciences, 17951) containing an anti-fade reagent (p-Phenylenediamine ...
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bioRxiv - Cell Biology 2020Quote: EVs were incubated overnight (4°C) with polystyrene microspheres 4.5 µm (Polysciences INC, Warrington, PA). The microspheres were blocked with 2% BSA (v/w ...
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bioRxiv - Microbiology 2020Quote: ... and both the apical and basal parts were fixed with 4% paraformaldehyde (PFA) (Polysciences, Inc.), and blocked with 10% goat serum (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips with the hippocampal cultures were mounted with Mowiol 4–88 antifade medium (Polysciences Europe). Neuronal images were acquired using an Olympus IX81 inverted epifluorescence microscope at 63× optical magnification with a CCD camera (Princeton MicroMax ...
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bioRxiv - Microbiology 2022Quote: ... The cells were trypsinized and fixed in 4% paraformaldehyde/0.01% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 ...
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bioRxiv - Microbiology 2022Quote: ... and both the apical and basal parts were fixed with 4% paraformaldehyde (PFA) (Polysciences, Inc.) in 1x PBS for 30 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were fixed in cold 4% paraformaldehyde (PFA) (Polysciences, Inc. Warrington, PA, USA) at room temperature for 20 min and washed with staining medium (PBS containing 0.1% NaN3 and 2% FCS) ...
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bioRxiv - Microbiology 2020Quote: ... The fixed suspension of bacteria was washed in sodium cacodylate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h ...
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Novel amiloride derivatives that inhibit bacterial motility across multiple strains and stator typesbioRxiv - Microbiology 2021Quote: ... and incubated for 10 mins and washed with motility buffer and flushed with 1 μm polystyrene bead (Polysciences, Inc) solution ...
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bioRxiv - Immunology 2021Quote: ... 480µg of each plasmid was co-transfected with 120µg of BirA (Howarth et al., 2008) and 12mg PEI-Max (1 mg/mL solution, Polysciences) in the presence of 200 µM biotin (final concentration) ...
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bioRxiv - Neuroscience 2021Quote: ... Day 1 adults were placed on a 10% agarose pad and immobilized with a slurry of polystyrene beads (Polysciences). A 1KHz train of 100 fs pulses (15-30 nJ/pulse ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were cultured in T75 flasks and transfected at approximately 70% confluency with 7500 ng of the lentiviral vector of interest and packaging plasmids pCMV-dR8.91 (6750 ng) and pCMV-VSV-G (750 ng) with 75 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... pSIREN- or pMXs-based plasmids were co-transfected together with pVSVG into Plat-E cells (kindly provided by T. Kitamura, University of Tokyo) using PEI Max (Mw 40,000, 24765-1; Polysciences). The virus-containing supernatant was harvested 2 days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... and sorting efficiency was verified manually by sorting a specified number of 1-μm yellow-green microspheres (Polysciences, USA) onto a glass slide and counting the beads under an epifluorescence microscope ...
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bioRxiv - Microbiology 2022Quote: ... B16F10 cells (2,500,000 cells) were transfected with 5 μg S expression plasmid by PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The cells at 1.5*106 confluence were transiently transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated at 37°C with 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: Prior to flow cytometry analysis 2 μl of fluorescent microspheres (1×10−3 of the stock Fluoresbrite® YG Microspheres 0.75μm, Polysciences) was added and the volume was adjusted to 500 μl by adding FACSFlow solution (10 mM phosphate-buffered saline ...
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bioRxiv - Microbiology 2020Quote: ... was performed by transfecting HEK293T cells in five 15 cm tissue culture plates using PEI (Polyethylenimin Linear, MW 25,000, 23966-1-A, Polysciences) transfection method (45) ...
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bioRxiv - Biochemistry 2020Quote: ... For formation of DNA:PEI complexes 1 μg DNA/mL transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
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Fast and sensitive diffuse correlation spectroscopy with highly parallelized single photon detectionbioRxiv - Neuroscience 2020Quote: Our decorrelating tissue phantom was created by a 1 μm polystyrene microsphere-in-water suspension (Polysciences, inc.; Warrington, PA) in custom made cuvettes ...
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bioRxiv - Molecular Biology 2022Quote: L1 and L4 larvae were mounted on a 10% agarose pad and immobilized in a 1-2 μL suspension of 0.1 μm polystyrene beads (Polysciences), as described in (Kim et al ...
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bioRxiv - Biophysics 2022Quote: ... The cells in each well were transiently transfected with 1 μg HcKCR1/2-CGFP DNA plasmid using 3μg linear polyethylenimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with 1 μg DNA/ml cells in a 3:2 (LC:HC) ratio were transfected using polyethylenimine HCl MAX (Polysciences). After 4 to 5 days of expression ...
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bioRxiv - Cell Biology 2022Quote: ... PRMT6-HA or Pu.1 as indicated using Polyethylenimine according to the manufacturer’s protocol (PEI, Polysciences, Cat#24765-2)
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Immunology 2022Quote: ... expi293F cells were transfected with antibody expression plasmids at a density of 2.5 million cells per/ml using 1 mg/ml PEI-Max transfection reagent (Polysciences). Supernatants were harvested 4-5 days post-transfection and tested for their SARS-CoV-2 RBD binding potential by enzyme-linked immunosorbent assay (ELISA) ...
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bioRxiv - Neuroscience 2024Quote: Animals were immobilized on a pad composed of 4% ultrapure agarose in H2O in 1-2 µL of 5 mM levamisole in H2O or in a suspension of 0.1 µm polystyrene beads (Polysciences) in H2O ...
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bioRxiv - Cell Biology 2024Quote: ... or with GFP-tau and vectors pCDNA3 overexpressing Cdk5 and p25 (1:0.85:0.15) using linear Polyethylenimine (PEI; Polysciences, Inc.). Cells were harvested 48 hours after transfection by centrifugation and flash-frozen ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... the remained solvent was blotted and three drops of 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was applied on the grid for staining purpose ...
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bioRxiv - Microbiology 2023Quote: HIV-1 viruses were generated by transfection of proviral DNA into HEK293T cells via polyethylenimine (PEI, Polysciences, Cat. #23966) transfection protocol ...
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bioRxiv - Biophysics 2023Quote: ... The cells in each well were transiently transfected with 1 μg DNA plasmid using 3μg linear polyethyleneimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Imaging was carried out by placing adult worms in 1.5 μl of M9 mixed with 1.5 μl of 1 μm polysterene microspheres (Polysciences Inc.) on a coverslip which was mounted carefully onto a 5% agarose pad.
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bioRxiv - Biochemistry 2023Quote: ... Flp-In Trex cells were co-transfected with a 1:9 ratio (w/w) of GFP vector:pOG44 Flp recombinase vector using PEI Max 40k (Polysciences). To select for integrant cells ...
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bioRxiv - Bioengineering 2023Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed MBV samples were incubated on the grids for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... followed by brief nuclear counterstaining using Hoechst (1:10,000) in PBS and mounting on a glass slide with Aqua-Poly-Mount (Polysciences).
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bioRxiv - Microbiology 2023Quote: ... Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in a sterile aqueous suspension without sodium azide ...
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bioRxiv - Neuroscience 2024Quote: ... the left sides of the brains were placed (anterior portion facing downwards) in disposable cryomold (Polysciences Inc. #18986– 1), and frozen in O.C.T ...
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bioRxiv - Neuroscience 2024Quote: ... the transfer and pDGM6 packaging plasmids were co-transfected in 293T cells (ATCC) using PEI reagent (Polysciences, 23966-1), as described previously 52 ...
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bioRxiv - Microbiology 2024Quote: ... Transient DNA transfections were performed using linear poly(ethylenimine) (1 mg/mL of solution in 10 mM Tris-HCl, pH 6.8; Polysciences), Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... 8.3 µg psPAX and 2.5 µg pMD2.G were mixed with 80 µg PEI MAX 40K (Polysciences, 24765–1) in 1.5 ml of Expi293 media ...
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bioRxiv - Neuroscience 2024Quote: ... Cells at ∼80% confluency were transfected with DNA plasmids using polyethyleneimine max according to the manufacturer’s manual (PEI Max; 24765-1, Polysciences). For CaST experiments ...
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bioRxiv - Bioengineering 2024Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed EV samples were incubated on the grids for 20 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.5-1 μg/mL of each construct was transfected with 5 μg/mL polyethyleneimine (PEI-25K; Polysciences, 23966). 24 hr after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... pEGFP-N1-RHBDL4 was transfected as a 1:10 mix with empty pcDNA3.1 plasmid into Hek293T cells (ATTC) using 25-kDa linear polyethylenimine (Polysciences) as had been described (Knopf et al ...
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bioRxiv - Developmental Biology 2024Quote: ... A second droplet of (∼ 10 µl) of a solution of 1 µm polystyrene microspheres (Polysciences, cat. no. 07310-15) was then added and gently mixed ...
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bioRxiv - Developmental Biology 2021Quote: Dechorionated and anesthetized embryos were fixed at desired stages in 4% methanol-free FA (Polysciences, 040181) overnight at 4°C and stored in 100% methanol at -20°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and chemically fixed by re-suspending in a buffer containing 4% formaldehyde (Polysciences, Inc., Warrington, USA), 80 mM HEPES-K ...