Labshake search
Citations for ChromoTek :
201 - 250 of 398 citations for Poly rC Binding Protein 4 PCBP4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: eYFP-tagged fusion proteins were immunoprecipitated using anti-GFP magnetic agarose beads (Chromotek, gtma-20). After induction of gene expression of lentiviral transduced KO2 cells with 1 µg/ml doxycycline ...
-
bioRxiv - Plant Biology 2022Quote: ... 100 μg SIRK1-GFP protein extract was prebound to GFP-Traps®-MA beads (Chromotek) for two hours under rotation at 4 °C ...
-
bioRxiv - Microbiology 2019Quote: ... Cleared lysate (700 µL, approximately 0.8 mg total proteins) were incubated with GFP-Trap (Chromotek) for 30 min at room temperature under gentle rotation ...
-
bioRxiv - Genetics 2019Quote: ... For IP 25 μL of Red Fluorescent Protein (RFP)-Trap Magnetic Agarose (MA) beads (Chromotek) were added and 25 μL of MA beads (Chromotek ...
-
bioRxiv - Cell Biology 2020Quote: ... GFP-tagged proteins were immunoprecipitated using the GFP-Trap Agarose® beads (Chromotek; gta-20) according to the manufacture’s protocol and eluted with 2x Laemmli Sample Buffer (BioRad ...
-
bioRxiv - Cancer Biology 2020Quote: ... Lysates from cells expressing GFP tagged proteins were immunoprecipitated using a GFP-Trap Kit (Chromotek) as per manufacturer’s instructions and immunoblotting performed as above ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were immunoprecipitated by the addition of 50 μl pre-equilibrated GFP-Trap resin (Chromotek) and inverted for 2 hours at 4 °C ...
-
bioRxiv - Neuroscience 2022Quote: Target proteins were immunoprecipitated either with GFP- or RFP-trap Magnetic Agarose beads (Chromotek, Proteintech) or with Dynabeads Protein G (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2023Quote: ... MYC-tagged protein was purified from transduced cells (see above) with Myc-trap agarose (Chromotek), a nanobody based system ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates incubated on a nutator at 4°C for two hours with 20μl of GFP-Trap beads (ChromoTek). GFP-Trap complexes were then washed four times in IP buffer and re-suspended in 2× SDS-PAGE sample buffer ...
-
bioRxiv - Cell Biology 2019Quote: ... lysates were incubated for 2 hours at 4°C with 25 μL GFP-Trap coupled agarose beads (Chromotek). After incubation ...
-
bioRxiv - Microbiology 2019Quote: ... and then diluted 1/4 in dilution buffer with 0.025% Tween-20 and added to washed GFP-Trap_MA beads (Chromotek). For the negative control ...
-
bioRxiv - Molecular Biology 2021Quote: ... Lysate was cleared by centrifugation and incubated for 2h at 4°C with magnetic GFP-Trap agarose (Chromotek). The beads were washed 3 x 10 min with Buffer 3 and were either used for mass spectrometry analysis or the proteins were eluted in 1× SDS buffer with 5 min incubation at 95°C for western blotting ...
-
bioRxiv - Genomics 2020Quote: ... at 4°C or for 2 hours with 20 uL of GFP-Trap magnetic beads (Chromotek, gtma-20) at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... and incubated for 1h at 4 °C before being added to 10 µl magnetic V5 beads (v5tma, Chromotek), pre-equilibrated in buffer K ...
-
bioRxiv - Plant Biology 2020Quote: ... A rat monoclonal anti-RFP antibody (Chromotek, cat. no. 5F8) was used at 1:1000 dilution and combined with a 1:5000 donkey anti-rat secondary antibody (Agrisera ...
-
bioRxiv - Neuroscience 2022Quote: ... and immunoblotted with a monoclonal primary antibody against mNeonGreen (Chromotek) at room temperature for 80 min and secondary antibody 680nm-anti rabbit (Jackson ImmunoResearch Laboratories Inc. ...
-
bioRxiv - Plant Biology 2021Quote: ... The antibodies used in Western blots were anti-GFP (Chromotek), anti-FLAG M2 (Sigma) ...
-
bioRxiv - Immunology 2019Quote: ... and MCM2 were incubated with turbo-GFP trap_MA antibody (Chromotek) or control binding antibody (bmab-20 ...
-
bioRxiv - Biophysics 2022Quote: ... The antibody was anti-GFP (1:1000, Chromotek Cat# 3H9). Secondary horseradish peroxidase conjugated antibodies were from Dako Cytomation ...
-
bioRxiv - Plant Biology 2022Quote: ... and then incubated with anti-GFP antibodies (Rat monoclonal, ChromoTek) diluted 1/1500 in TBS containing 0.1% Tween overnight at 4C ...
-
bioRxiv - Biochemistry 2023Quote: ... The primary antibody was anti-GFP (Chromotek, 3H9 3h9-100). The secondary antibody was HRP-anti-rat (Invitrogen ...
-
bioRxiv - Genomics 2023Quote: ... GFP using the rabbit polyclonal antibody padg1 (1:2000, ChromoTek), tubulin using the monoclonal α-alpha tubulin TEU435 (1:10000 or 1:5000 ...
-
bioRxiv - Neuroscience 2024Quote: ... labeled using primary antibody (mouse anti-mNEONGreen, 1:1000, ChromoTek) and secondary antibody (goat anti-mouse conjugated with Alexa Fluor 555 ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein (1 mg/mL) was incubated with 25 μL of GFP-Trap magnetic micro beads (ChromoTek), or anti-FLAG M2 magnetic beads (Sigma) ...
-
bioRxiv - Microbiology 2021Quote: ... Protein extracts were then subjected to immunoprecipitation using agarose beads coupled with anti-RFP nanobodies (Chromotek). Eluates were collected and analysed by western blot ...
-
bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated for 1 h with 25 μl GFP trap (ChromoTek; Planegg-Martinsried, Germany) on a rotating wheel at 4°C ...
-
bioRxiv - Plant Biology 2019Quote: ... Total proteins were extracted and immunoprecipitation was performed with GFP-trap beads (Chromotek, Planegg-Martinsried, Germany). Proteins were stripped from the beads by boiling in 50 μl SDS loading buffer for 10 mins ...
-
bioRxiv - Cell Biology 2022Quote: ... with equal amounts of total protein extract for each condition incubated with GFP-Trap_A beads (Chromotek) at 4°C for 1 hour ...
-
bioRxiv - Microbiology 2022Quote: ... to equalise protein concentrations across the samples before immunoprecipitation with GFP-Trap resin (gta-20, ChromoTek) or anti-HA affinity matrix (11815016001 ...
-
bioRxiv - Plant Biology 2022Quote: ... leaf material was harvested and protein extracts were subjected to immunoprecipitation using GFP-Trap beads (ChromoTek). 200 mg ground leaf material was subjected to protein extraction and immunoprecipitation using GFP-Trap beads (ChromoTek)19 ...
-
bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 mg total proteins were incubated with 15 µl pre-equilibrated magnetic GFP-TRAP beads (Chromotek) for 2 h on a rotator at 4 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... 1.5 mg of protein was mixed with 25 μl of pre-equilibrated GFP-Trap_A beads (ChromoTek) and incubated for 2 hours at 4°C with gentle agitation ...
-
bioRxiv - Cell Biology 2022Quote: ... EGFP-INPP5E or its mutants were then immunoprecipitated from 1 mg of cell lysate by overnight rotation at 4°C with GFP-Trap_MA beads (Chromotek). Beads were then washed thrice in buffer containing 50mM Tris-HCl pH=7.5 and 150mM NaCl buffer (no protease inhibitors or detergent) ...
-
bioRxiv - Cell Biology 2019Quote: ... The homogenate was clarified by centrifugation at 750 g for 15 min at 4°C and the supernatant was incubated with 200 μl of GFP-Trap_MA magnetic beads (ChromoTek) for 4 h on a rotating wheel at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... for 30 min at 4°C and then with GFP-Trap Magnetic Particles M-270 (gtd-20, ChromoTek GmbH) for 1 h at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 16,000 g for 5 min at 4 °C before it was immobilized on 20 μl GFP-Trap beads (ChromoTek) for 30 min at 4 °C ...
-
bioRxiv - Biophysics 2023Quote: ... 0.25 mM PMSF) and eluted with 4 × 1 mL 0.15 mg/mL (50 µM) 3 x FLAG peptide (Chromotek). The elutant was concentrated with Amico-4 (50k MWCO ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was cleared at 14,000 rpm for 10 min and incubated for 10 min rotating at 4 °C together with 12.5 μl pre-equilibrated GFP-Trap beads (Chromotek). Beads were washed four times with GFP pull-down buffer at 2,500 g for 2 min at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... Supernatants were centrifuged at 21,000 g at 4 °C for 10 min and then incubated for 10 min at 4 °C with 12.5 μl pre-equilibrated GFP-Trap beads (Chromotek) rotating ...
-
bioRxiv - Plant Biology 2024Quote: ... Lysates were cleared by filtering through miracloth followed by centrifugation (6,000 g, 30 min, 4°C) and then incubated with GFP-Trap (Chromotek) for 4h ...
-
bioRxiv - Molecular Biology 2019Quote: ... GFP-tagged proteins were immunoprecipitated by incubation with 25 µl of GFP-Trap magnetic agarose beads (Chromotek) overnight at 4 °C on a tube rotator ...
-
bioRxiv - Plant Biology 2021Quote: ... Protein extraction and immunoprecipitation were performed as described previously (Kadota et al., 2014) using GFP-trap (Chromotek) or GFP-clamp (Hansen et al. ...
-
bioRxiv - Plant Biology 2021Quote: ... The protein extracts were incubated with 20 μL of GFP-Trap magnetic agarose beads (GFP-TrapMA, Chromotek) for 2 h at 4 °C by vertical rotation (10 rpm) ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were immunoprecipitated by incubation with anti-HA sepharose beads (Pierce) or anti-GFP-Trap beads (ChromoTek) for 2 h at 4°C ...
-
bioRxiv - Plant Biology 2021Quote: ... To each 15 ml of protein extract 40 μl of GFP-TRAP AGAROSE BEADS (50 % slurry, ChromoTek) washed in extraction buffer were added and incubated with gentle agitation for 4 h at 4 °C ...
-
bioRxiv - Systems Biology 2020Quote: ... were proportioned for analysis of protein expression (5%) and immunoprecipitation (95%) using GFP-Trap beads (ChromoTek, Germany). Co-immunoprecipitated proteins were detected using anti-Flag antibody (M2 – Sigma) ...
-
bioRxiv - Neuroscience 2022Quote: ... Overexpressed protein was immunoprecipitated from the cell lysate using tag specific nano-trap (SPOT-Trap-MA, Chromotek) and analysed by WB.
-
bioRxiv - Cell Biology 2021Quote: ... cleared lysates containing GFP-tagged proteins were added to anti-GFP agarose beads (GFP-Trap, ChromoTek, Germany) and incubated while rotating end over end for an hour at 4°C ...