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Citations for ChromoTek :
1 - 50 of 398 citations for Poly rC Binding Protein 4 PCBP4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2019Quote: ... or control binding antibody (bmab-20, Chromotek). For tight chromatin fractionation ...
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bioRxiv - Biophysics 2020Quote: ... they were incubated with unlabelled (GFP-binding protein, Chromotek) and Abberior 635P conjugated Nanobody (GFP-booster ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
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bioRxiv - Cell Biology 2020Quote: IPs with GFP-tagged proteins were performed with GFP-binding magnetic agarose beads (GFPtrap MA, Chromotek, #gtma-20). Per IP sample ...
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bioRxiv - Cell Biology 2024Quote: ... The insoluble material was removed by centrifugation and the lysates were subjected to immunoprecipitation either by subsequent incubations with anti-Ecad antibody SHE78-7 (4 μg/ml) and protein A-beads or by incubation with GFP-trap beads (Chromotek). After incubation ...
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bioRxiv - Cell Biology 2021Quote: ... and binding control agarose beads (Chromotek).
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bioRxiv - Microbiology 2019Quote: ... binding control magnetic agarose beads (Chromotek) were used ...
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bioRxiv - Plant Biology 2021Quote: ... thaliana plants were incubated with 50-100 μl previously washed 50% GFP-binding protein beads slurry (Chromotek, GFP-Trap®) at 4 °C for 3 hours on a roller mixer ...
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bioRxiv - Developmental Biology 2024Quote: ... To avoid unspecific protein binding the worm lysate was pre-cleared by incubation to uncoupled agarose beads (Chromotek, bab-20) for 1 hour at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was subsequently used for the Co-IP using a GFP-binding protein coupled to magnetic beads (GFP-Trap_M; ChromoTek, Planegg-Martinsried) following manufacturers instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... or binding control beads (Chromotek #bmab-20). The immune complexes were run on SDS-PAGE and analyzed by Western blotting.
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bioRxiv - Cell Biology 2022Quote: ... 10 µl of GFP-binding agarose beads (Chromotek) were incubated with the protein extract for 1 hour at 4 °C in head-over-shoulder rotation ...
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bioRxiv - Plant Biology 2024Quote: ... a negative control (binding control agarose beads, Chromotek) and input control were used ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were detected using anti-GFP antibody (3H9, Chromotek) or anti-alpha tubulin (T5168 ...
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bioRxiv - Plant Biology 2020Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Systems Biology 2021Quote: ... Protein concentration was evaluated with a BCA dosage and 1 mg of total protein was incubated at 4 °C for 4 hours with agarose GFP beads (ChromoTek, Germany) for PHB-GFP or with magnetic FLAG beads (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... or the 32F6 IgG2c monoclonal antibody against mNeonGreen protein (Chromotek). After several 5 min washes in PBS ...
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bioRxiv - Plant Biology 2024Quote: ... GFP-tagged proteins were detected by α-GFP antibodies (ChromoTek).
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bioRxiv - Microbiology 2021Quote: ... 25 μl of binding control agarose beads (Chromotek; bab20), prepared in dilution buffer (10 mM Tris-Cl pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... or with binding control agarose beads (Chromotek #bab-20) using RIPA lysis buffer and according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was precleared with binding control agarose beads (ChromoTek) for 1hr at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Plant Biology 2021Quote: ... the protein–chromatin solution was incubated with GFP antibody-conjugated magnetic beads (ChromoTek) under 4°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... total proteins were incubated with 10 μl agarose conjugated anti-GFP antibodies (Chromotek) for 3 h and washed 3 times with the washing buffer (50 mM HEPES [pH 7.5] ...
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bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were immunoprecipitated for 1 h at 4°C using GFP-Trap beads (ChromoTek, Planegg, Bavaria, Germany) following the method of Chinchilla et al5 or for Fig ...
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bioRxiv - Cell Biology 2021Quote: ... the proteins were detected by Western blot analyses with the indicated antibodies (GFP (Chromotek) 1:4,000 ...
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bioRxiv - Plant Biology 2019Quote: ... RFP-fused proteins were detected using a rat anti-RFP antibody (1:5,000; ChromoTek). Light harvesting complex protein (LhcB1 ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were then incubated for 1.5 h at 4 ⁰C with 25 µL RFP-Trap agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were left on ice for 30 minutes and centrifuged at 16,000 g for one hour at 4 °C and the protein was captured with 25 μL Myc-trap (Chromotek) beads by tumbling overnight ...
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bioRxiv - Plant Biology 2019Quote: ... mCherry proteins were detected by a rat anti-RFP antibody (5F8-100, 1:2,000, Chromotek), and then with a goat anti-rat IgG HRP-conjugated secondary antibody (AP202P ...
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bioRxiv - Plant Biology 2020Quote: ... 50μg of total proteins were incubated 3H at 4°C with 30μl of GFP-Trap Agarose beads (Chromotek; gta-20) under gentle agitation for GFP-tagged protein purification ...
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bioRxiv - Cell Biology 2024Quote: ... 0.6 mL of the resulting supernatant (containing 2 – 4 mg of proteins) were supplemented with 15 μL of RFP-Trap magnetic particles (ChromoTek), and incubated for 2.5 h at 4°C on a rotating wheel ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated overnight at 4°C with primary antibody (mouse anti-mNeonGreen [32F6], Chromotek, 1:250) diluted in PBT + 1x WBR ...
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bioRxiv - Plant Biology 2023Quote: ... GFP tagged proteins were detected using an anti-GFP antibody (1:4000; mAb rat 3H9; Chromotek) and a secondary anti-rat antibody (1:15000 ...
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bioRxiv - Biochemistry 2020Quote: ... Diluted lysate was pre-cleared by incubation with binding control agarose beads (bab-20, CHROMOTEK), at a volume of slurry equivalent to 1:200 (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were incubated for 1 hr with 20 μl of agarose binding control beads (ChromoTek), equilibrated in IP buffer 2 (200 mM NaCl ...
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bioRxiv - Biophysics 2019Quote: ... Presence of the mNeonGreen protein was probed using a monoclonal mNeonGreen antibody [32F6] (Chromotek; Planegg-Martinsried, Germany) at a 1:1000 dilution and visualized using a DyLight 680 secondary antibody (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 30 μl GFP-Trap Agarose or Binding Control Agarose Beads (Chromotek, gta-10 and bab-20) were washed once in dilution buffer (10mM tricHCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 100 mg of total protein (4 ml of extract) were immunoprecipitated with 25 µl of GFP-Trap magnetic agarose beads (Chromotek gtma-20) following incubation for 2 h at 4°C in a rotating wheel ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at +4°C with the primary antibody (rabbit anti-GFP, 1:600, Chromotek) diluted in the blocking solution ...
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bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 4 mgs of protein extract per sample was then incubated with GFP-Traps beads (ChromoTek; 3 μl/mg of extract) for 3 hours at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was precleared in 20 μl binding control magnetic beads (bmp-20, ChromoTek; Munich, DE) for 30 min at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... The blot was incubated overnight on a shaker (4°C) with the primary antibody (anti-GFP 3H9, anti-RFP 6G6 (Chromotek) or anti-tRFP (Evrogen)) ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...