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Citations for ChromoTek :
401 - 450 of 454 citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cells were fixed and treated for immunofluorescence with α-SINV C antibody at 1:200 dilution and GFP-Booster_Atto488 (ChromoTek GmbH, #gba488-100) at 1:500 dilution following the procedure described in (Garcia-Moreno et al. ...
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bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
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bioRxiv - Neuroscience 2022Quote: ... TH-GFP signal (after activity imaging) was amplified using a rabbit anti-GFP (1:2000; Chromotek PABG1) antibody ...
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bioRxiv - Biophysics 2021Quote: ... Cells were further incubated on ice with membrane impermeable Alexa Fluor 647– labeled anti-GFP nanobodies (Chromotek) for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... Twenty μl of anti-GFP beads was mixed with 1.4 ml of filtered extracts (Chromotek, Munich, Germany) and incubated at 4□ for 2 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were further incubated on ice with membrane impermeable Alexa Fluor 647– labelled anti-GFP nanobodies (Chromotek) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... cleared lysates containing GFP-tagged proteins were added to anti-GFP agarose beads (GFP-Trap, ChromoTek, Germany) and incubated while rotating end over end for an hour at 4°C ...
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bioRxiv - Biochemistry 2022Quote: Expressed GFP-ALDH9A1 was enriched using anti-GFP beads following the manufacturer’s protocol for GFP-Trap (Chromotek). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... The signal of mCherry and GFP was enhanced using a rat mAb anti-RFP (Chromotek, 5f8-100) and a rabbit pAb anti-GFP antibody (BIOZOL/MBL ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight on a Nutator with anti-GFP bound agarose beads (Chromotek GFP-Trap® #gta). Lysates and beads were separated in a centrifuge ...
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bioRxiv - Pathology 2024Quote: ... The supernatants obtained from the samples were incubated with 10 µL anti-GFP magnetic beads (Chromotek, Germany) for three hours under gentle agitation at 4℃ ...
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bioRxiv - Biochemistry 2021Quote: ... immunoprecipitation was performed using either GFP-Trap (high affinity anti-GFP alpaca nanobody bound to agarose beads; Chromotek) or anti-HA beads (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: The input and elution samples obtained after immunoprecipitation using anti-GFP beads (Miltenyi or agarose beads from Chromotek). If necessary ...
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bioRxiv - Cell Biology 2024Quote: ... Samples (inputs and eluates) were further analysed by western blot with rat anti-GFP (1:1000, 3H9, Chromotek) and rabbit anti-Fbxo42 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Co-IP’s on the total cell lysates or on subcellular fractions (nuclear and cytoplasmic) were performed at different timepoints using anti-GFP VHH coupled to magnetic agarose beads (GFP-Trap_MA, Chromotek) or ...
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bioRxiv - Neuroscience 2020Quote: ... 2 and 3 GAGs fusion proteins were first concentrated by immunoprecipitation of roughly 20,000 nematodes per genotype using Trap-A bead coupled with anti-mNG nanobody (Chromotek). 1.5 U of heparanase I and III (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... The precleared lysate was incubated with 30 μl of magnetic agarose beads coupled to anti-GFP nanobodies (GFPTrap; ChromoTek) for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Single-molecule tracking was carried out in modified Tyrode’s solution supplement with 0.8% BSA and ATTO647N-conjugated anti-GFP nanobodies (1:15000 dilution, GFPBooster-ATTO647N, Chromotek, #gba647n) on Nanoimager microscope (Oxford Nanoimaging ...
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bioRxiv - Developmental Biology 2021Quote: ... blocked as above and incubated in primary (rat anti-GFP, 1:200, ChromoTek, Cat. No. 3h9-100, clone 3H9) diluted in block as above ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was blocked using BlueBlock PF (SERVA) and developed using rat monoclonal anti-RFP 5F8 (1:2000, Chromotek) and rabbit anti-rat-peroxidase A9542 (1:20000 ...
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bioRxiv - Plant Biology 2022Quote: ... The ribosylated NbFBN1a2A-GFP and NbFBN1a-GFP were detected by the anti-pan-ADP-ribose binding reagent after they were pulled down with Co-IP by GFP-Trap_A (ChromoTek).
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bioRxiv - Microbiology 2020Quote: ... and then incubated with 25 μl anti-HA magnetic beads (Pierce) or with 25 μL GFP-Trap Magnetic Agarose (Chromotek) overnight at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... The filtered supernatant was then incubated for one hour at 4°C with 50 μL of anti-GFP agarose beads (Chromotek) to pull down GRP7-GFP and with 50 μL of anti-HA agarose beads (ThermoFisher ...
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bioRxiv - Plant Biology 2020Quote: ... Small cuts from the infiltrated regions were incubated for one hour in anti-Spot-Tag VHH/Nanobodies conjugated to the organic fluorophore ATTO488 (Chromotek), diluted 1:1000 in 10 mM Tris-HCl solution pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... then mixtures were centrifuged (15 min, 4º, 14000 rcf) and the supernatant (input) was added to washed anti-GFP beads (Chromotek). Beads and lysates were incubated 2-4 h at 4 °C while rotating ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek, Germany, Cat. No. gtma-20) for 3h at 4°C while rotating ...
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bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
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bioRxiv - Developmental Biology 2021Quote: ... Vibratome sections prepared from inflated and fixed lobes at 350 µm thickness were stained with chicken anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
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bioRxiv - Developmental Biology 2021Quote: ... Lungs were collected at 5 days after tamoxifen injection and vibratome sections (350 µm) were stained with rat anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was then aspirated without disturbing the upper lipid layer and split by half into either the anti-GFP agarose beads or the control blocked beads (22 μl, Chromotek). After head over tail rotation at 4°C for 90 min ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-GFP VHH coupled to agarose beads for immunoprecipitation of GFP-fusion proteins (GFP-Trap; ChromoTek GmbH, Germany; gta-20) was used for immunoprecipitation of mCerN1 ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated with 25 μl of anti-HA magnetic beads (Pierce) or with 25 μl of GFP-Trap Magnetic Agarose (ChromoTek) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was then transferred to new collection tubes and incubated with to 30 µl of prewashed anti-myc or -V5 beads (Chromotek) and equilibrated to a final volume of 1000 µl by adding lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: Samples for STED microscopy were prepared by incubating yeGFP-tagged Cdc3 cells (wild-type CDC11 or mutants) with a 1/250 dilution of an anti-GFP nanobody Atto 647N (ChromoTek), as previously detailed in (52) ...
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bioRxiv - Plant Biology 2023Quote: ... Immunogold labelling of CASP1-GFP was carried out according to [45] using a 1:5 dilution of rat monoclonal anti-GFP 3H9 (Chromotek) and a 1:20 dilution of goat anti-rat IgG conjugated to 10 nm colloidal gold particles (British Biocell International) ...
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bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were incubated with a 5-μL slurry of anti-Spot nanobody-coupled magnetic agarose beads (Spot-Trap, ChromoTek) for 3 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed with a buffer containing 1% Triton X-100 for 20 min and the lysate was incubated with anti-GFP magnetic beads (Chromotek) for 2 hours in 0.33% Triton X-100 solution ...
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bioRxiv - Neuroscience 2024Quote: ... In order to amplify the endogenous PSD95-mVenus fluorescence signal brain sections were immunostained with an anti-GFP Nanobody coupled to ATTO 647N (GBA647N-100, ChromoTek). For that brain sections were washed for 10 minutes in Phosphate Buffer (PB ...
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bioRxiv - Cell Biology 2024Quote: ... cell lysates were incubated for 1.5h at room temperature with anti-myc-Trap Magnetic Beads (ChromoTek GmbH, Planegg-Martinsried, Germany). After two washing steps (10 mM Tris/Cl pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... transduced cells were incubated with 100 μL Hybridization Buffer containing 2 μL 12.5 μM CF568-labled gRNA FISH probes and 1:2000 AF647-labeled anti-GFP nanobody (Chromotek, gb2AF647-50) for 4 hours in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... Then 800-1000 µg of total protein lysates were added to 25 μl of anti-GFP magnetic beads (ChromoTek GFP-Trap) and tumbled end-over-end for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: For CoIP experiments 500 μl protein extract was mixed with 25 μl calibrated anti-GFP magnetic beads (ChromoTek GFP-Trap®) and incubated on a rotator for 2 h ...
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bioRxiv - Pathology 2020Quote: ... aliquots of the supernatants equivalent to 2 x 108 cells were incubated with 10 μL anti-Myc agarose beads (Myc-Trap™, Chromotek) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and approximately 250 µg of protein was incubated with anti-GFP-conjugated magnetic agarose beads (ChromoTek GFP-Trap® Magnetic Agarose) for 1-1.5 hours ...
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bioRxiv - Genetics 2023Quote: ... for 30 min to eliminate the background signal, and then incubated with anti-HP1α (NB110-40623) (Novus, St Louis, MO, USA) or GFP-Trap (gta-20) (Chromotek, Germany) antibodies at 4 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... mEGFP-mAID-NIPBL and SCC1-mEGFP were detected and visualised with an anti-GFP nanobody booster (Chromotek, gba488-100, 1:250).
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation of tagged PC1 was done using anti-GFP nanobodies coupled to magnetic agarose beads (GFP-trap, Chromotek cat. no. gtma-10) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were blocked for 30 min in 1x Roti-block solution (Carl Roth GmbH, Karlsruhe) before applying rabbit anti-GFP 1:1000 (ChromoTek GmbH, Martinsried) over night at 4 °C in the same solution ...
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bioRxiv - Systems Biology 2024Quote: vDISCO was performed as previously described2,30 in combination with GFP-Nanobooster labeling (Atto647N-conjugated anti-GFP nanobooster Chromotek Cat.# gba647n-100;RRID:AB_2629215) for 6 days and passive labeling for 3 days ...