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Citations for ChromoTek :
351 - 400 of 454 citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and immunoblots were performed with rat anti-HA (1:1000, 7C9, Chromotek) antibody.
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bioRxiv - Plant Biology 2021Quote: ... For the localization of RNAPII we used antibodies recognizing RNAPII phosphorylated at serine 5 (Chromotek, diluted 1:200) and serine 2 (Chromotek ...
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bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Further primary antibodies used for western blots in this manuscript are as follows: monomeric NeonGreen (1:1’000, Chromotek), phospho-eIF2α (1:1’000 ...
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bioRxiv - Biophysics 2023Quote: ... The membrane was air-dried for 1 h and immunoblotted with a primary antibody against mNeonGreen tag (Chromotek) at room temperature for 1 h followed by a secondary antibody (680nm anti-mouse ...
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bioRxiv - Plant Biology 2023Quote: ... GFP was detected using a rat 3H9 GFP-specific polyclonal antibody (diluted 1:1000)(ref 3H9-100ab, Chromotek, Germany ...
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bioRxiv - Microbiology 2020Quote: ... 1% FCS for 30 min and immune-stained with anti-GFP nanobody (Chromotek) conjugated in-house to AlexaFluor647-NHS (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... The immunoprecipitation (IP) step was carried out using an anti-GFP beads (Chromotek). Five independent experiments for DHX34 were performed using DHX34-GFP-FLAG A5 clone with parental HEK293T serving as negative control ...
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bioRxiv - Neuroscience 2023Quote: ... The lysates were subjected to anti-GFP immunoprecipitation using GFP- Trap beads (ChromoTek). The presence of GFP and GFP-PGRN in immunoprecipitated samples was confirmed by SDS-PAGE and silver staining ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µl of either anti-GFP Trap magnetic beads (Chromotek Inc. Cat# gtma) or 1D4-Ab-conjugated sepharose beads (∼30% slurry ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were immunostained with rat anti-RFP (1:800, Chromotek, recognizing mCherry) together with guinea pig anti-c-Fos (AB_2619946 ...
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bioRxiv - Microbiology 2020Quote: ... incubated O/N in primary antibody (Rabbit Pan-ADPr 1:1000, Cell Signaling E6F6A; Rabbit GFP 1:1000, Chromotek PABG1-100 ...
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bioRxiv - Physiology 2022Quote: ... 2mg/ml was used per IP experiments performed with camelid antibodies GFP-Trap Magnetic Agarose (Cat# gtma-20, Chromotek); RFP-Trap Magnetic Agarose (Cat# rtma-20 ...
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bioRxiv - Neuroscience 2022Quote: ... Another set of sections was incubated in a mixture of the following primary antibodies: rat-RFP (1:1,000, Chromotek), rabbit anti-somatostatin (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Neuroscience 2022Quote: ... Sections ipsilateral to the injection side were incubated in a mixture of the following primary antibodies: rat-RFP (1:1,000, Chromotek), rabbit anti-somatostatin (1:10,000 ...
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bioRxiv - Cell Biology 2024Quote: ... A 30% suspension of protein A sepharose beads in RIPA buffer were incubated with the mNG antibody (32F6 Chromotek) for 1 hr at 4 °C ...
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bioRxiv - Genetics 2021Quote: ... Pulldowns of the GFP-fusion proteins were performed by incubating tissue homogenate (7-day-old seedlings) or cell lysate (CHO cell lysate) with agarose beads that conjugated with GFP antibodies (GFP Nano-Trap, Chromotek). After incubations of 4-24 hours with gentle inverting ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with primary antibody solution containing rabbit a-HA (1:500) (Cell Signalling) and rat a-RFP (1:500) (Chromotek) diluted in 3% BSA in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunoprecipitation was performed on clarified lysates using anti-GFP (GFP-Trap agarose beads, Chromotek) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 mM DTT was incubated with 25 µl of anti-GFP agarose beads (Chromotek) (Wu et al. ...
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bioRxiv - Biophysics 2022Quote: ... Anti-GFP nanobody used to label FAK-eGFP was from Chromotek (Chromotek: gt-250) and was conjugated to JaneliaFluor 646 through incubation with JF646 NHS ester (gift of Luke Lavis ...
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bioRxiv - Neuroscience 2021Quote: ... and anti-GFP nanobodies conjugated with ATTO647N (1:15000 dilution, GFPBooster-ATTO647N, Chromotek, #gba647n). The following secondary antibodies were used ...
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bioRxiv - Developmental Biology 2022Quote: ... 150 μl of anti-GFP nanobody conjugated to magnetic beads (Chromotek; cat# gtma-10) were incubated with the lysates at 4°C for 90 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatant was added to 1.5 mg of anti-V5- conjugated magnetic beads (Chromotek). The solubilized mitochondria were incubated under rotation with the beads for 90 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... The rest of the lysate was incubated with the HK2 antibody-conjugated beads or GFP-Trap® Magnetic Agarose (ChromoTek, #gtma) overnight at 4°C while constantly rotating ...
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bioRxiv - Cell Biology 2020Quote: ... anti-GFP conjugated to Atto488 (1:100; ChromoTek gba-488; Fig. 3B-C, S2A,C), anti-mouse secondary antibodies (1:400 ...
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bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was then incubated with anti-GFP coupled magnetic microparticles (GFP-Trap, ChromoTek) for 2 h followed by four washing steps ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated with 5% (w/v) milk powder-TBST including anti-GFP (Chromotek) as primary antibody (diluted to1:1000 ...
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bioRxiv - Cell Biology 2022Quote: eYFP-tagged fusion proteins were immunoprecipitated using anti-GFP magnetic agarose beads (Chromotek, gtma-20). After induction of gene expression of lentiviral transduced KO2 cells with 1 µg/ml doxycycline ...
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bioRxiv - Cell Biology 2020Quote: ... anti-RFP (Invitrogen, #R10367, 1:1000 for WB and Chromotek #5F8, 1:1000 for WB), anti-phospho myosin light chain (Cell Signaling Technology ...
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bioRxiv - Systems Biology 2022Quote: ... and 1:500 anti-rabbit nanobody coupled to Alexa Fluor 647 (Chromotek srbAF647-1-100). Slides were washed three times for two minutes in PBS in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... the RFP-Booster Alexa Fluor 568 (alpaca monoclonal anti-RFP Nanobody; 1:1000; rb2AF568, ChromoTek) was used with second antibodies to amplify the signal of NLS-sfCherry.
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bioRxiv - Cell Biology 2022Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were then added to 75 uL (slurry) of anti-V5 magnetic resin (ChromoTek) and let rotate at 4°C for 2 hrs ...
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bioRxiv - Neuroscience 2024Quote: ... sections were stained with rat anti-red fluorescent protein (RFP) (1:800, mAB 5F8, Chromotek) and then IRDye® 800CW goat anti-rat secondary antibody (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Total cell extracts were analysed by immunoblotting with rat anti-GFP (1:1000, 3H9, Chromotek) and rabbit anti-Fbxo42 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... GFP-Trap Magnetic Agarose beads or control beads without antibodies were used according to the manufactured protocol (both Chromotek, Planegg-Martinsried, Germany). The loaded magnetic beads were heated for 10 min at 95 °C in SDS loading buffer for the dissociation of purified proteins ...
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bioRxiv - Plant Biology 2021Quote: ... For immunoprecipitations, α-rabbit Trueblot agarose beads (eBioscience) coupled with α-FLS2 antibodies (Chinchilla et al., 2007) or GFP-Trap agarose beads (ChromoTek) were used and incubated with the crude extract for 3-4 h at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Protein extracts were then subjected to immunoprecipitation using agarose beads coupled with anti-RFP nanobodies (Chromotek). Eluates were collected and analysed by western blot ...
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bioRxiv - Plant Biology 2021Quote: ... Anti-GFP coated beads were added (25 μL ChromoTek GFP-Trap or 50 μL MACS magnetic) and IP was carried out as per the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was subjected to immunoprecipitation for 1 hour using anti-GFP (ChromoTek, Planegg- Martinsried Germany), anti-RFP (ChromoTek ...
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bioRxiv - Plant Biology 2023Quote: ... 600 µL of plant lysate was incubated with 30 µL of anti-mRFP magnetic beads (Chromotek) in a rotary mixer for 1 hrs at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... each supernatant was incubated with 50 µL of Anti-cMyc agarose beads (Chromotek, 004282-07-02) at 4°C for 4 h ...
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bioRxiv - Biochemistry 2023Quote: ... The rest of the supernatant was mixed with 8 µL anti-GFP beads (Chromotek, gta-20). The mixture was incubated for 2 h at 4 °C while rotating ...
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bioRxiv - Microbiology 2024Quote: ... The precleared lysate was incubated overnigh with an anti-GFP-Trap-A beads (ChromoTek, gta-20) antibody ...
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bioRxiv - Neuroscience 2024Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
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bioRxiv - Cell Biology 2024Quote: ... Xbp1s-GFP protein levels were analyzed by immunoblot with rat anti-GFP (1:1000, 3H9, Chromotek) and tubulin was used as loading control ...