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Citations for ChromoTek :
351 - 382 of 382 citations for Indusatumab Anti GUCY2C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: Samples for STED microscopy were prepared by incubating yeGFP-tagged Cdc3 cells (wild-type CDC11 or mutants) with a 1/250 dilution of an anti-GFP nanobody Atto 647N (ChromoTek), as previously detailed in (52) ...
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bioRxiv - Cell Biology 2024Quote: ... The insoluble material was removed by centrifugation and the lysates were subjected to immunoprecipitation either by subsequent incubations with anti-Ecad antibody SHE78-7 (4 μg/ml) and protein A-beads or by incubation with GFP-trap beads (Chromotek). After incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was then transferred to new collection tubes and incubated with to 30 µl of prewashed anti-myc or -V5 beads (Chromotek) and equilibrated to a final volume of 1000 µl by adding lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-GFP VHH coupled to agarose beads for immunoprecipitation of GFP-fusion proteins (GFP-Trap; ChromoTek GmbH, Germany; gta-20) was used for immunoprecipitation of mCerN1 ...
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bioRxiv - Cell Biology 2023Quote: ... portions of the blot were reacted against anti- mNeonGreen (mouse monoclonal antibody 32F6 from Chromotek, Inc., at 1:200 dilution), anti- HA (rabbit monoclonal antibody ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed with a buffer containing 1% Triton X-100 for 20 min and the lysate was incubated with anti-GFP magnetic beads (Chromotek) for 2 hours in 0.33% Triton X-100 solution ...
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bioRxiv - Developmental Biology 2023Quote: ... The CUT and TAG experiment was done for two biological replicates using antibodies anti-GFP (Chromotek, PABG1-100, 1:50), anti-YFP (Merck Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... Immunogold labelling of CASP1-GFP was carried out according to [45] using a 1:5 dilution of rat monoclonal anti-GFP 3H9 (Chromotek) and a 1:20 dilution of goat anti-rat IgG conjugated to 10 nm colloidal gold particles (British Biocell International) ...
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bioRxiv - Neuroscience 2024Quote: ... In order to amplify the endogenous PSD95-mVenus fluorescence signal brain sections were immunostained with an anti-GFP Nanobody coupled to ATTO 647N (GBA647N-100, ChromoTek). For that brain sections were washed for 10 minutes in Phosphate Buffer (PB ...
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bioRxiv - Cell Biology 2024Quote: ... cell lysates were incubated for 1.5h at room temperature with anti-myc-Trap Magnetic Beads (ChromoTek GmbH, Planegg-Martinsried, Germany). After two washing steps (10 mM Tris/Cl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... 1:150 and Alex Fluor 488 conjugated site-specific dual monoclonal alpaca anti rabbit nano secondaries (single domain antibodies, VHHs, Chromotek) 1:1000 in blocking solution was applied to the sections at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... transduced cells were incubated with 100 μL Hybridization Buffer containing 2 μL 12.5 μM CF568-labled gRNA FISH probes and 1:2000 AF647-labeled anti-GFP nanobody (Chromotek, gb2AF647-50) for 4 hours in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... Then 800-1000 µg of total protein lysates were added to 25 μl of anti-GFP magnetic beads (ChromoTek GFP-Trap) and tumbled end-over-end for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: For CoIP experiments 500 μl protein extract was mixed with 25 μl calibrated anti-GFP magnetic beads (ChromoTek GFP-Trap®) and incubated on a rotator for 2 h ...
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bioRxiv - Microbiology 2021Quote: ... Slides were subsequently blocked overnight in PBS containing 3% BSA before labelling with anti-mNeonGreen mouse monoclonal antibody (32F6; 1:300; Chromotek, Germany) followed by Alexa Fluor 488 goat anti-mouse antibody (1:1000 ...
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bioRxiv - Pathology 2020Quote: ... aliquots of the supernatants equivalent to 2 x 108 cells were incubated with 10 μL anti-Myc agarose beads (Myc-Trap™, Chromotek) for 1 h at 4 °C ...
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bioRxiv - Genetics 2023Quote: ... for 30 min to eliminate the background signal, and then incubated with anti-HP1α (NB110-40623) (Novus, St Louis, MO, USA) or GFP-Trap (gta-20) (Chromotek, Germany) antibodies at 4 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and approximately 250 µg of protein was incubated with anti-GFP-conjugated magnetic agarose beads (ChromoTek GFP-Trap® Magnetic Agarose) for 1-1.5 hours ...
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bioRxiv - Cell Biology 2023Quote: ... mEGFP-mAID-NIPBL and SCC1-mEGFP were detected and visualised with an anti-GFP nanobody booster (Chromotek, gba488-100, 1:250).
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Mutual repression between JNK/AP-1 and JAK/STAT stratifies cell behaviors during tissue regenerationbioRxiv - Developmental Biology 2022Quote: ... Membrane was incubated with primary antibodies in PBS-T (1% Triton-X in 1xPBS) - rat anti-GFP (Chromotek, 3H9-100, 1:1000) and mouse anti-α-tubulin (Sigma ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation of tagged PC1 was done using anti-GFP nanobodies coupled to magnetic agarose beads (GFP-trap, Chromotek cat. no. gtma-10) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were blocked for 30 min in 1x Roti-block solution (Carl Roth GmbH, Karlsruhe) before applying rabbit anti-GFP 1:1000 (ChromoTek GmbH, Martinsried) over night at 4 °C in the same solution ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were clarified by centrifugation at 12,000 rpm at 4°C for 10 min and then mixed with anti-RFP antibody coupled agarose beads (RFP-Trap, ChromoTek, Martinsried, Germany) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Systems Biology 2024Quote: vDISCO was performed as previously described2,30 in combination with GFP-Nanobooster labeling (Atto647N-conjugated anti-GFP nanobooster Chromotek Cat.# gba647n-100;RRID:AB_2629215) for 6 days and passive labeling for 3 days ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C) and incubated with the equilibrated anti-GFP nanobody conjugated to magnetic particles (Chromotek GFP-Trap® Magnetic Particles M-270) for 1 h on a rotator disk at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... One ml of the supernatant was recovered in a new tube containing 25 µl of sepharose beads covalently attached to anti GFP nanobodies (VHH) (ChromoTek. Cat. Nº: gta). Samples were incubated in a rotating wheel for 60 minutes at 4ºC ...
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bioRxiv - Cell Biology 2024Quote: ... thawed 90-nm-thick cryosections were incubated for 30 min at RT with a rabbit anti-GFP antibody (Chromotek, PABG1, 1:200 dilution) followed by protein A conjugated to 10 nm gold particles for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... brucei MEX67 polyclonal antiserum (1:1000, kind gift of Mark Carrington) and anti-GFP nanobody (GFP-Booster Alexa Fluor 647 Chromotek gb2AF647, 1:1000).
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then probed overnight at 4°C with TBST buffer containing 1% non-fat dry milk and primary antibodies: mouse monoclonal anti-RFP (Chromotek, clone 6G6, 1:3,000), rabbit polyclonal anti-GFP (SYSY ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then transferred in 900 μl aliquots to fresh Eppendorf tubes and incubated with gentle head-over-tail agitation for 2 h at 4°C with 30 μl of anti-RFP or control beads (Chromotek rtma-2 and bmab-20, respectively) that had been washed in 1x lysis buffer.