Labshake search
Citations for ChromoTek :
301 - 350 of 382 citations for Indusatumab Anti GUCY2C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... The supernatant was subjected to immunoprecipitation for 1 hour using anti-GFP (ChromoTek, Planegg- Martinsried Germany), anti-RFP (ChromoTek ...
-
bioRxiv - Plant Biology 2023Quote: ... 600 µL of plant lysate was incubated with 30 µL of anti-mRFP magnetic beads (Chromotek) in a rotary mixer for 1 hrs at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... The rest of the supernatant was mixed with 8 µL anti-GFP beads (Chromotek, gta-20). The mixture was incubated for 2 h at 4 °C while rotating ...
-
bioRxiv - Neuroscience 2024Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
-
bioRxiv - Cell Biology 2024Quote: The following primary antibodies were used at the indicated dilutions: alpaca anti-GFP Booster (ChromoTek, gb2AF488) was used at 1:1000 ...
-
bioRxiv - Microbiology 2024Quote: ... The precleared lysate was incubated overnigh with an anti-GFP-Trap-A beads (ChromoTek, gta-20) antibody ...
-
bioRxiv - Neuroscience 2024Quote: ... was used at a 1:2,000 dilution and the primary anti-RFP monoclonal antibody (Chromotek, 6G6) was used at a 1:1,000 dilution ...
-
bioRxiv - Cell Biology 2024Quote: ... Cell lysates were then subjected western blotting probed by mouse anti-RFP (6g6, Chromotek, IL, USA) and mouse anti-α-tubulin antibodies (TAT-1 ...
-
bioRxiv - Cell Biology 2024Quote: ... Xbp1s-GFP protein levels were analyzed by immunoblot with rat anti-GFP (1:1000, 3H9, Chromotek) and tubulin was used as loading control ...
-
bioRxiv - Developmental Biology 2021Quote: ... and then incubated overnight at 4°C with primary antibody (mouse anti-mNeonGreen [32F6], Chromotek, 1:250) diluted in PBT + 1x WBR ...
-
bioRxiv - Neuroscience 2022Quote: ... TH-GFP signal (after activity imaging) was amplified using a rabbit anti-GFP (1:2000; Chromotek PABG1) antibody ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were further incubated on ice with membrane impermeable Alexa Fluor 647– labeled anti-GFP nanobodies (Chromotek) for 30 min ...
-
bioRxiv - Plant Biology 2022Quote: ... Twenty μl of anti-GFP beads was mixed with 1.4 ml of filtered extracts (Chromotek, Munich, Germany) and incubated at 4□ for 2 hours ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were further incubated on ice with membrane impermeable Alexa Fluor 647– labelled anti-GFP nanobodies (Chromotek) for 30 min ...
-
bioRxiv - Cell Biology 2021Quote: ... cleared lysates containing GFP-tagged proteins were added to anti-GFP agarose beads (GFP-Trap, ChromoTek, Germany) and incubated while rotating end over end for an hour at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: Expressed GFP-ALDH9A1 was enriched using anti-GFP beads following the manufacturer’s protocol for GFP-Trap (Chromotek). Briefly ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 15 µl of Myc-Trap Agarose (with anti-Myc alpaca antibody) (Chromotek, cat. No. yta) and incubated for 3 h at 4°C with end-over-end rotation ...
-
bioRxiv - Cell Biology 2023Quote: ... The signal of mCherry and GFP was enhanced using a rat mAb anti-RFP (Chromotek, 5f8-100) and a rabbit pAb anti-GFP antibody (BIOZOL/MBL ...
-
bioRxiv - Neuroscience 2024Quote: ... then incubated overnight on a Nutator with anti-GFP bound agarose beads (Chromotek GFP-Trap® #gta). Lysates and beads were separated in a centrifuge ...
-
bioRxiv - Pathology 2024Quote: ... The supernatants obtained from the samples were incubated with 10 µL anti-GFP magnetic beads (Chromotek, Germany) for three hours under gentle agitation at 4℃ ...
-
bioRxiv - Biochemistry 2021Quote: ... immunoprecipitation was performed using either GFP-Trap (high affinity anti-GFP alpaca nanobody bound to agarose beads; Chromotek) or anti-HA beads (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2023Quote: The input and elution samples obtained after immunoprecipitation using anti-GFP beads (Miltenyi or agarose beads from Chromotek). If necessary ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples (inputs and eluates) were further analysed by western blot with rat anti-GFP (1:1000, 3H9, Chromotek) and rabbit anti-Fbxo42 (1:1000 ...
-
bioRxiv - Cell Biology 2020Quote: ... Co-IP’s on the total cell lysates or on subcellular fractions (nuclear and cytoplasmic) were performed at different timepoints using anti-GFP VHH coupled to magnetic agarose beads (GFP-Trap_MA, Chromotek) or ...
-
bioRxiv - Neuroscience 2020Quote: ... 2 and 3 GAGs fusion proteins were first concentrated by immunoprecipitation of roughly 20,000 nematodes per genotype using Trap-A bead coupled with anti-mNG nanobody (Chromotek). 1.5 U of heparanase I and III (Sigma ...
-
bioRxiv - Cancer Biology 2021Quote: ... The precleared lysate was incubated with 30 μl of magnetic agarose beads coupled to anti-GFP nanobodies (GFPTrap; ChromoTek) for 1 hr at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... mCherry and GFP or EYFP signals in the neurons were enhanced by immunostainings using the following antibodies: rat anti-RFP (1:1,000, Chromotek) revealed by Cy3-conjugated donkey anti-rat secondary antibody (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: Single-molecule tracking was carried out in modified Tyrode’s solution supplement with 0.8% BSA and ATTO647N-conjugated anti-GFP nanobodies (1:15000 dilution, GFPBooster-ATTO647N, Chromotek, #gba647n) on Nanoimager microscope (Oxford Nanoimaging ...
-
bioRxiv - Developmental Biology 2021Quote: ... blocked as above and incubated in primary (rat anti-GFP, 1:200, ChromoTek, Cat. No. 3h9-100, clone 3H9) diluted in block as above ...
-
bioRxiv - Plant Biology 2021Quote: ... The membrane was blocked using BlueBlock PF (SERVA) and developed using rat monoclonal anti-RFP 5F8 (1:2000, Chromotek) and rabbit anti-rat-peroxidase A9542 (1:20000 ...
-
bioRxiv - Plant Biology 2022Quote: ... The ribosylated NbFBN1a2A-GFP and NbFBN1a-GFP were detected by the anti-pan-ADP-ribose binding reagent after they were pulled down with Co-IP by GFP-Trap_A (ChromoTek).
-
bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at +4°C with the primary antibody (rabbit anti-GFP, 1:600, Chromotek) diluted in the blocking solution ...
-
bioRxiv - Microbiology 2020Quote: ... and then incubated with 25 μl anti-HA magnetic beads (Pierce) or with 25 μL GFP-Trap Magnetic Agarose (Chromotek) overnight at 4 °C ...
-
bioRxiv - Genetics 2020Quote: ... The supernatant was transferred to a fresh tube and incubated with anti-GFP antibody coupled with magnetic agarose (GFP-Trap, Chromotek) overnight at 4 °C on a rocking platform ...
-
bioRxiv - Molecular Biology 2021Quote: ... They were then incubated with antibody-bead conjugate (Anti-FLAG® M2 Magnetic Beads, Millipore & Sigma or GFP-Trap_A, Chromotek) overnight at 4 °C ...
-
bioRxiv - Plant Biology 2021Quote: ... The filtered supernatant was then incubated for one hour at 4°C with 50 μL of anti-GFP agarose beads (Chromotek) to pull down GRP7-GFP and with 50 μL of anti-HA agarose beads (ThermoFisher ...
-
bioRxiv - Plant Biology 2020Quote: ... Small cuts from the infiltrated regions were incubated for one hour in anti-Spot-Tag VHH/Nanobodies conjugated to the organic fluorophore ATTO488 (Chromotek), diluted 1:1000 in 10 mM Tris-HCl solution pH 7.5 ...
-
bioRxiv - Plant Biology 2020Quote: ... The roots were incubated overnight at 4°C with the secondary anti-mouse (1:500; Jackson immunoreactive, conjugated to Rhodamine Red) and eba488-10 Spot Label ATTO488 (1:1000, Chromotek) diluted in PBS supplemented with 2% (w/v ...
-
bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... then mixtures were centrifuged (15 min, 4º, 14000 rcf) and the supernatant (input) was added to washed anti-GFP beads (Chromotek). Beads and lysates were incubated 2-4 h at 4 °C while rotating ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek, Germany, Cat. No. gtma-20) for 3h at 4°C while rotating ...
-
bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
-
bioRxiv - Developmental Biology 2021Quote: ... Vibratome sections prepared from inflated and fixed lobes at 350 µm thickness were stained with chicken anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
-
bioRxiv - Developmental Biology 2021Quote: ... Lungs were collected at 5 days after tamoxifen injection and vibratome sections (350 µm) were stained with rat anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
-
bioRxiv - Pathology 2021Quote: ... sections were blocked and antibodies were incubated at 4°C overnight which was either primarily conjugated with Alexa Fluor 488-conjugated dual monoclonal recombinant alpaca anti-rabbit IgG nano secondaries (Chromotek) or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories ...
-
bioRxiv - Cell Biology 2021Quote: ... or to affinity purify ribosomes with translated RNA using GFP-TRAP beads (magnetic agarose beads coated with Alpaca anti-GFP VHH antibody, Chromotek). Incubation with the GFP-TRAP beads proceeded for 3 h at 4°C with gentle end-over-end mixing in a tube rotator and was followed by three washes with high salt buffer (350 mM KCl ...
-
bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was then aspirated without disturbing the upper lipid layer and split by half into either the anti-GFP agarose beads or the control blocked beads (22 μl, Chromotek). After head over tail rotation at 4°C for 90 min ...
-
bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
-
bioRxiv - Microbiology 2022Quote: ... the supernatants were incubated with a 5-μL slurry of anti-Spot nanobody-coupled magnetic agarose beads (Spot-Trap, ChromoTek) for 3 h at 4 °C ...
-
bioRxiv - Microbiology 2024Quote: ... and then incubated with 25 μl of anti-HA magnetic beads (Pierce) or with 25 μl of GFP-Trap Magnetic Agarose (ChromoTek) overnight at 4°C ...