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Citations for Invivogen :
51 - 100 of 151 citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... NK cell activating medium was supplemented with 10 ng/mL IL-12 (Invivogen), 50 ng/mL IL-15 ...
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bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was added IL-1β Reporter HEK 293 Cells (Invivogen cat. hkb-il1bv2) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mouse TLR2 neutralizing antibody monoclonal mouse IgG2a (C9A12) and control isotypes mouse IgG2a were from InvivoGen. Mouse TLR4/MD2 complex neutralizing antibody monoclonal rat IgGk clone MTS510 and control isotypes rat IgGk were from eBioscience™ Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: Wild-type and mutants MmEsco2-myc/his and H2B-mCherry were cloned into the pVITRO2-hygro-mcs vector (InvivoGen) in two steps ...
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bioRxiv - Molecular Biology 2021Quote: ... + 4 μg/ml Blasticidin (Invivogen). In order to knock-out SLX4 gene in these cells ...
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bioRxiv - Developmental Biology 2020Quote: ... 4 μg/μL Zeocin (Invivogen) were added to culture media ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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bioRxiv - Immunology 2023Quote: ... stimulated monocytes were partially pre-incubated with a neutralizing IL-1α antibody (1:1,000, Invivogen, USA) for 1h ...
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bioRxiv - Immunology 2023Quote: ... These analyses were conducted using either a HEK293T TLR4-CD14-MD2 IL-18 reporter line (Invivogen) or the RAW-Blue Cell assay (Invivogen) ...
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bioRxiv - Physiology 2019Quote: ... and 4 µg/ml blasticidin (InvivoGen). Monoclonal cell lines were tested for robust expression of E2GFP and FaNaC by fluorescence microscopy and patch clamp electrophysiology ...
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bioRxiv - Genomics 2024Quote: ... cells were stimulated for 45 minutes with 30 ng/ml human recombinant IL-1α (rcyec-hil1b; Invivogen) or 30 ng/ml human recombinant TNF-α (rcyc-htnfa ...
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bioRxiv - Molecular Biology 2022Quote: ... and 4 μg ml-1 puromycin (InvivoGen), and grown at 37 °C in an atmosphere containing 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... Pam3CSK4 (100 µg/mouse; InvivoGen), or Poly(I:C ...
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bioRxiv - Microbiology 2020Quote: ... then incubated with 1 µM okadaic acid 30 min before transfer onto transgenic IL-1R reporter cells (Invivogen). After 18 h ...
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bioRxiv - Immunology 2021Quote: ... 4 µg cGAMP (Invivogen, San Diego, Californien, USA), 4 µg c-di-UMP (Invivogen ...
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bioRxiv - Immunology 2020Quote: ... for 4 hours and 10 μM nigericin (InvivoGen) for an additional 1 hour ...
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bioRxiv - Immunology 2023Quote: ... and assayed with QUANTI-Luc 4 reagent (Invivogen). Luminescence was read immediately with an Envision system.
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bioRxiv - Immunology 2023Quote: ... selective medium containing Puromycin (4 μg/ml, InvivoGen) was added and after 7 days cells were sorted using FACS Aria (BD ...
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bioRxiv - Immunology 2023Quote: ... cell culture supernatant was analyzed for NF-κB/AP-1-induced SEAP production by using QUANTI-Blue reagents and IL-8-dependent expression of Lucia luciferase using QUANTI-Luc reagents (both Invivogen). For QUANTI-Blue measurement ...
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bioRxiv - Microbiology 2024Quote: ... A positive control for TNF-α or IL-1β release was also prepared by challenging cells with 1 μg/ml lipopolysaccharide (Escherichia coli 0111:B4; InvivoGen) for an equivalent time ...
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bioRxiv - Immunology 2020Quote: ... and selected with 4 μg mL−1 puromycin (InvivoGen) for two weeks.
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bioRxiv - Immunology 2020Quote: ... ovalbumin (OVA) EndoFit (5 μg/mouse) and Alhydrogel adjuvant 2% (alum, 100 μg/mouse) were purchased from Invivogen; recombinant hemagglutinin (rHA ...
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bioRxiv - Microbiology 2020Quote: ... with 50 μl/mouse of AddaVax (Invivogen) as the adjuvant ...
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bioRxiv - Immunology 2023Quote: ... or Poly(I:C) (100 µg/mouse; InvivoGen) in 200 µl PBS for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... IL-18 signaling activity was measured in 50 µL volumes of cell supernatant using HEK-Blue™ IL-18 Reporter cells (Invivogen) and secreted alkaline phosphatase activity measured after 18 h incubation as previously [56] ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were obtained from ATCC (catalog # CRL-3216) and HEK-Blue-IL-1β cells were obtained from Invivogen (catalog # hkb-il1b) and all lines were verified by those sources ...
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bioRxiv - Cell Biology 2020Quote: ... Stable transformants were selected using 4 μg/mL Blasticidin (Invivogen) and individual colonies were picked ...
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bioRxiv - Immunology 2021Quote: ... 4 µg c-di-UMP (Invivogen, San Diego, Californien, USA) or mock-electroporated using a Gene Pulser Xcell Electroporation instrument (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2023Quote: ... or the mouse kappa light chain plasmid (InVivoGen). The gBlock DNA and plasmids were cut with the specified enzymes ...
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bioRxiv - Immunology 2020Quote: ... The coding sequences of the 4A3 heavy chain variable region and light chain variable region were amplified by adding the IL-2 signal peptide and cloned into the expression vectors pFUSE-CHIg-HG1 and pFUSE2-CLIg-hk (Invivogen, San Diego, CA), respectively ...
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bioRxiv - Immunology 2020Quote: The VRC01 LC-P2A-HC construct was PCR-amplified from engineered C57BL/6J mouse cDNA and cloned between the promoter and constant regions of a mouse IgG2b heavy chain expression vector (InvivoGen, #pfuse-mchg2b) using Gibson Assembly ...
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bioRxiv - Immunology 2019Quote: ... a Mouse TLR Agonist kit was purchased from Invivogen and BMDMs were treated with each ligand for 16 hours ...
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bioRxiv - Systems Biology 2023Quote: ... TLR4 agonist at 4 ng/mL (LPS-B5, Invivogen, San Diego, CA), BTC at 40 ng/mL (human recombinant Betacellulin protein ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µM digitonin) with or without 7 µM 2’,3’-cGAMP (Invivogen). Cells were further washed in PBS and incubated in culture medium until collection ...
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bioRxiv - Immunology 2023Quote: ... cells were transferred to selective medium containing 4 µg/mL Blasticidin (Invivogen) (MC38-B2m-/- transduced with Her2/neu ...
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bioRxiv - Cell Biology 2022Quote: ... ASC-expressing RAW264.7 mouse macrophages (RAW-ASC; InvivoGen raw-asc) are engineered to stably express murine ASC ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibodies used: Mouse anti-HA antibody (InvivoGen, 1:1000), rabbit anti-ACP (1:2000) ...
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bioRxiv - Immunology 2021Quote: ... Mouse IFN-α was measured by a bioluminescence kit (InvivoGen). All assays were performed on cell free supernatants according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... or 200 µg of isotype control antibody (mouse IgG2a, Invivogen) three times at days 6 ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse STING activator DMAXX and cGAMP were purchased from InvivoGen.
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bioRxiv - Immunology 2023Quote: ... mice were immunised with VacOva (vac-pova, InvivoGen; 50μg/mouse) and LPS (L6529 Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse NIK expression plasmid was purchased from Invivogen (pUNO1-mMap3k14). Wild-type human NIK and the NIK-K429/430A mutant plasmids were a gift from Prof ...
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bioRxiv - Cancer Biology 2024Quote: ... or 10 mg/kg anti-CTLA-4 (clone 9D9, cat. mctla4-mab10, InvivoGen). Antibodies were administered twice weekly for a maximum of four weeks.
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bioRxiv - Immunology 2020Quote: ... the cells were primed for 4 h with 500 ng/mL ultrapure LPS (InvivoGen). The supernatant was discharged and macrophages were infected with trypomastigote forms of T ...
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bioRxiv - Immunology 2019Quote: ... HBV Pol and HBcAg in pVAC1 vector (Invivogen, 50 μg/mouse ea.); the group of mice immunized with empty vector VLV received empty pVAC1 vector (150 μg/mouse) ...
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bioRxiv - Immunology 2020Quote: ... mouse RAW macrophages (RAW-Lucia ISG, RAW-Lucia ISG-KO-cGAS, Invivogen) were cultured in DMEM (Thermo Fisher cat ...
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bioRxiv - Immunology 2023Quote: ... mouse NIH/3T3 fibroblasts were transfected with NotI-linearized pUNO1 vectors (Invivogen) encoding human BAFF (CD257 ...
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bioRxiv - Immunology 2023Quote: ... LPS-primed mouse BMDMs were transfected with 2 μg poly(dA:dT) (InvivoGen) using Lipofectamine 3000 and incubated for 6 h.
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bioRxiv - Biochemistry 2023Quote: ... and 50 µL of the working solution of the QUANTI1Luc 4 Lucia/Gaussia reagent (Invivogen) was added ...