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Citations for Invivogen :
101 - 150 of 158 citations for Rat Colony Assay Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: For IL-1ß assay HEK-Blue™ IL-1β reporter cells (Invivogen, #hkb-il1bv2) were used ...
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bioRxiv - Bioengineering 2022Quote: ... The injector was primed with QUANTI-Luc assay solution (Invivogen, cat. code rep-qlc1), and the measurement was taken ...
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bioRxiv - Bioengineering 2023Quote: ... and the QUANTI-Blue™ assay kit were obtained from Invivogen (San Diego, CA). BrightGLO™ was purchased from Promega (Madison ...
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bioRxiv - Biochemistry 2023Quote: Jurkat-Lucia TCR-hPD-1 effector cells and Raji-APC-hPD-L1 antigen presenting cells were obtained from Invivogen as components of the PD-1/PD-L1 Bio-IC assay (Invivogen, #rajkt-hpd1). The cells were cultivated in IMDM with glutamine and HEPES (Gibco ...
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bioRxiv - Immunology 2020Quote: ... The K562 cell line was cultured in the same media than NK cells supplemented with 5 µg/mL of Plasmocin (InvivoGen) and was routinely tested for mycoplasma infection with Venor GeM Classic detection kit (Minerva Biolabs).
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bioRxiv - Cell Biology 2022Quote: ... U2OS media was replaced with fresh media and 24 hours later U2OS infected cells were selected with 2 µg/ml of puromycin (InvivoGen) for 72 hours.
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed in 1x phosphate buffer saline (PBS) and subsequently incubated with puromycin containing media (InvivoGen, 10 μg/ml) for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... The U2OS USP42 KO cell line and its complemented cell lines stably expressing either GFP or GFP-USP42 (FL, ΔC or Δ946-1196) were cultured with identical media with 1 μg/ml puromycin (InvivoGen) or with 1 μg/ml puromycin and 500 μg/ml Geneticin ...
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bioRxiv - Immunology 2019Quote: ... 10% L929-conditioned media and 1% P/S supplemented with 100 ng/mL crude LPS (InVivogen, San Diego, CA, USA) and 20 ng/mL IFNγ (R&D Systems ...
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bioRxiv - Pathology 2020Quote: ... The conditioned media from each treatment group was analyzed for the presence of SEAP using QUANTIBlue™ detection reagent (Invivogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 10 uL of conditioned media from the co-culture wells were mixed with 50 uL of the commercial Quanti-Luc reagent (Invivogen) in a black ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 1% sorbitol and either 50 mg/liter phleomycin (after adjusting the media to pH 7) or 200 mg/liter hygromycin Gold (both InvivoGen). For solid medium ...
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bioRxiv - Immunology 2020Quote: ... the activation of NF-κB drives the expression of the alkaline phosphatase enzyme that induces a color shift from pink to blue of the chromogenic substrate in the HEK-Blue Detection Media (Invivogen). These cells were cultured in complete DMEM medium composed of DMEM GlutaMAX (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Transfected HEK-Blue-KD-TLR5 cells were then gently flushed in PBS and resuspended in HEK-Blue Detection Media (Invivogen) at 2.8 × 105 cells/mL ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were seeded at 1 × 105 cells/well in 96-well U-bottom plates in media containing 500 ng/mL Pam3CSK4 (Invivogen) for 4-6 hours then treated with either Val-boroPro (10 μM ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transfected Cells were incubated for 72 hours before selection with media containing 2 ug/mL Puromycin (Invivogen ant-pr-1). Cells were selected for at least 3 passages before fluorescence activated cell sorting (FACS) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then pelleted (150 RCF, 5 min) and SEAP enriched supernatant was then utilized for detection media (QUANTI-Blue, Invivogen) activation.
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bioRxiv - Immunology 2023Quote: ... 20 µl of media from the THP-1 Dual cells was incubated with 180 µl Quanti-Blue (InvivoGen, # rep-qbs) and incubated for 4 hours at 37°C before quantification at OD635 ...
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bioRxiv - Immunology 2023Quote: ... followed by the medium being replaced with fresh IFNγ (5 ng/ml) containing media for 2 h before treatment with indicated stimulations: MDP (100 μM, Invivogen), iE-DAP (100 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... Three days post-transfection cells were sub-cultured into media supplemented with puromycin (0.8 µg/mL; Invivogen, ANT-PR-1). After six days of selection ...
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bioRxiv - Immunology 2023Quote: ... MACS-DRG neurons were resuspended in F12 media supplemented with 10% fetal bovine serum (FBS) and 100 µg/ml primocin (Invivogen) and plated on chamber slides (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... After 48-72h the medium containing viruses was replaced with fresh media with 1 μg/mL Puromycin or 1 μg/mL Hygromycin B Gold (InvivoGen), used for selection.
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bioRxiv - Immunology 2022Quote: Inhibition of spike-mediated cell-cell fusion was tested using an assay developed by Invivogen. Briefly ...
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bioRxiv - Synthetic Biology 2021Quote: SEAP was detected in the media and in the lysate with an alkaline phosphatase detection medium – powder QUANTI-Blue™ (InvivoGen) as per the products instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Matrigel/cell mix was incubated for 15 min at 37C to allow for solidification and base media with Primocin® (InvivoGen) was added to top and bottom chambers of transwell ...
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bioRxiv - Molecular Biology 2021Quote: ... Stable transformants were maintained in media supplemented with blasticidin and 50 μg/ml Hygromycin B Gold (InvivoGen, cat n°ant-hg-1).
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bioRxiv - Immunology 2021Quote: ... cells were incubated at 1×106 cells/mL T cell media plus 1 μg/mL phorbol myristate acetate (PMA) (Invivogen; tlrl-pma), 1μg/mL ionomycin (Calbiochem ...
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bioRxiv - Bioengineering 2021Quote: ... performing half media changes every 3-4 days with fresh NbActiv1 supplemented with PrimocinTM (ant-pm-1, InvivoGen, San Diego, California, USA). Cultures were incubated at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... ≈80% confluent adherent cells were washed twice in 1× PBS and subsequently pulsed with puromycin-containing media (InvivoGen, 10 μg mL−1) for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... 180 µl/well of cells at a concentration of 2.8 × 105 cells/ml in HEK-Blue detection media (Invivogen; catalog #hb-det2) were plated in 96 well tissue-culture plate ...
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bioRxiv - Cancer Biology 2023Quote: ... The transfection mix was applied to the cells for six hours then removed and replaced with lentiviral collection media: DMEM + 10% FBS(HyClone) + 1.1% BSA + HEPES (10 mM) + sodium pyruvate (10 mM) + Primocin (Invivogen ant-pm-1). The lentivirus was collected in two batches at 48 and 72 hours and pooled together ...
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bioRxiv - Bioengineering 2023Quote: ... 2×105 PBMCs and 4×104 autologous CD8+ T cells were incubated with either supernatants from engineered PCs or media containing recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) or anti-CD33 x anti-CD3 bispecific (AMG 330 ...
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bioRxiv - Bioengineering 2023Quote: ... 5×103 control cells and 5×104 CD8+ T cells were incubated with either various dilutions of supernatants from genome-engineered cells or media containing various concentrations of recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) for 48 hours (Figure 1F-H & 3G-I) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: PLpro inhibitor antiviral activities were tested in a cell culture assay with A549:hACE2 cells (Invivogen) by the following protocol ...
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bioRxiv - Microbiology 2023Quote: ... Full length RIG-I (pUNO-hRIG-I) used in the 5Br assay was obtained from InvivoGen. To generate the FLAG-pTBK1 plasmid ...
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bioRxiv - Developmental Biology 2024Quote: ... to prevent mycoplasma contamination during maintenance of the cells but was removed before any experimental assay (InvivoGen).
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bioRxiv - Biochemistry 2024Quote: NF-kB activation was determined by performing the QUANTI-Blue assay using the RAW-BlueTM cells (InvivoGen). Through exposure of the RAW-Blue cells to various substances ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... cells were washed in 1x phosphate buffer saline (PBS) and subsequently incubated with puromycin containing media (InvivoGen, San Diego, California, USA, 10 µg/ml) for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Levels of SEAP were detected in the culture media after 3 hours incubation of supernatants with Quanti-Blue solution (InvivoGen, San Diego, CA, USA) at 650nm wavelength by ELISA reader.
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bioRxiv - Immunology 2020Quote: ... Promoter induction was measured by the SEAP reporter secretion (quantified at 620nm following addition of Quanti-Blue™ media, Invivogen, per the manufacturer’s instructions).
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bioRxiv - Cell Biology 2022Quote: ... For KO generation 1 GFP-positive cell was placed in each wheel of a 96 multi-well plate containing complete media supplemented with plasmocin (15 µg / ml) (InvivoGen. Cat. Nº: ant-mpt). Cells were amplified until final confirmation of gene knockout by RT-PCR and protein electrophoresis followed by western blot as previously described (45) ...
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bioRxiv - Immunology 2021Quote: ... For inflammasome activation on the day of the assay cells were treated with 10 ng/mL LPS (Invivogen), followed by 1 ug/mL poly dA:dT (Invivogen ...
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bioRxiv - Microbiology 2023Quote: HEK-BlueTM hTLR2 secreted alkaline phosphatase (SEAP) reporter assays were performed according to manufacturer instructions (Invivogen, hkb-htlr2). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Cell lines were regularly checked for Mycoplasma contamination using the cell- based colometric HEK-Blue detection assay (Invivogen) and passaged maximally 20 times ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-PATC148 cells were obtained from MD Anderson Cancer Center and cultured in RPMI media supplemented with 2 uM glutamine and containing 100ug/mL primocin (Invivogen, Cat. no. ant-pm-1). Cell lines were routinely tested for mycoplasma and validated using STR profiling and mutational analysis.
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bioRxiv - Immunology 2021Quote: ... For rescue assays cells were treated at the time of LPS priming with 10 μM AC-YVAD-CMK (Invivogen), 1 mM GNE9605 (Cayman Chemicals) ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were verified as >95% pure by SDS-PAGE and contained <0.01 endotoxin units ml-1 by HEK-Blue TLR4 reporter assay (InvivoGen).
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bioRxiv - Genetics 2020Quote: ... Cells were lysed using passive lysis buffer and the activity of the enhancer assessed using a Lucia QUANTI-Luc Gold Assay #3 luciferase kit (InvivoGen). Because of clear evidence of the response of the pGL4.74 Renilla normalisation control plasmid to PMA treatments and co-transfection with the EGR1 expression vector Lucia activity levels were normalised against quantitative PCR (qPCR ...
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bioRxiv - Immunology 2022Quote: The different reagents used in this assay and their sources were: ultrapure Escherichia coli lipopolysaccharide (LPS) serotype 0111:B4 (InvivoGen), Adenosine 5’-triphosphate (ATP ...
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... Lucia and Cypridina luciferase activity was quantified by bioluminescence from aliquots of the cell supernatant (BioLux Cypridina Luciferase assay kit, New England Biolabs; Quanti-Luc, InvivoGen). Reference plasmid-normalized luciferase activity was from the average of three independent transfections ...