Labshake search
Citations for Invivogen :
1251 - 1300 of 1306 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: 1×106 freshly thawed PBMC were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) and Brefeldin A for 6 hours in 96-well tissue culture plates at 37C in a 5% CO2 environment ...
-
bioRxiv - Cancer Biology 2023Quote: ... The transfection mix was applied to the cells for six hours then removed and replaced with lentiviral collection media: DMEM + 10% FBS(HyClone) + 1.1% BSA + HEPES (10 mM) + sodium pyruvate (10 mM) + Primocin (Invivogen ant-pm-1). The lentivirus was collected in two batches at 48 and 72 hours and pooled together ...
-
bioRxiv - Bioengineering 2023Quote: ... the mice were intratumorally injected with 40 μg of STINGΔTM protein mixed with 1 μg of 2’3’-cGAMP (InvivoGen, Cat # tlrl-nacga23-02) in 25 μL sterile PBS ...
-
bioRxiv - Immunology 2023Quote: NF-κB/AP-1 activation in THP-1-XBlue-CD14 reporter monocytes was determined after 20– 24 h of incubation according to the manufacturer’s protocol (InvivoGen, San Diego, USA). Briefly ...
-
bioRxiv - Cell Biology 2024Quote: Differentiated CD11c+ CD1c+ cells from iPSC cell lines and CB donors were FACS sorted (Table 5) and stimulated with a combination of 1 µg/ml LPS (InvivoGen, #tlrl-smlps), 5 µg/ml HMW poly(I:C ...
-
bioRxiv - Cell Biology 2024Quote: ... Neutrophils were resuspended at 1×107 cells/mL in complete media w/o phenol red supplemented with 20 ng/ml GM-CSF and pulsed with 1 μg/ml LPS (InvivoGen, # tlrl-smlps) for 30 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were infected with virus in the presence of 5 µM polybrene for two days and subsequently selected either through antibiotic selection with 1 µg/ml puromycin (InvivoGen, ant-pr), 10 µg/ml blasticidin (InvivoGen ...
-
bioRxiv - Immunology 2022Quote: The following in vivo treatment regimens were used in this study: DMXAA (5,6-dimethylxanthenone-4-acetic acid, Invivogen): 20mg/Kg intraperitoneal (i.p.) ...
-
bioRxiv - Cell Biology 2021Quote: ... HeLa Cyclin B1-GFP cells were grown in media containing 4 μg/ml blasticidin (Invivogen # ant-bl-05). HeLa Cyclin A2-GFP cells 25 were grown in media containing 9 μg/ml blasticidin (gifts of Francis Barr) ...
-
bioRxiv - Cell Biology 2024Quote: ... Hygromycin selection plates were seeded with 4 mg/mL hygromycin B (Hygromycin B Gold, InvivoGen, HGG-44-04). Worm populations were synchronized by hypochlorite treatment (Bleach solution:2.8% bleach ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 days after transfection the cells were transferred to 10 cm dishes and 4-5 days after transfection cells were selected with 0.75-1.0 μg/ml Puromycin (InvivoGen). All CRISPaint plasmids are summarized in Table S1.
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were then sorted for GFP-positive to enrich for CROP-seq-vector-positive cells and plated on matrigel-coated plates in mTesr1supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and Y-27632 (final concentration 5µM) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Parent Flp-In™ T-REx™-293 cells were additionally supplemented with 15 μg/ml blasticidin (InvivoGen, cat n°ant-bl-1) and 100 μg/ml zeocin for cultivation ...
-
bioRxiv - Developmental Biology 2020Quote: ... and cells were re-plated to a 75 cm2 flask in culture medium supplemented with 20 µg/mL blasticidin (InvivoGen, ant-bl-1) to begin the antibiotic selection.
-
bioRxiv - Immunology 2020Quote: ... T cell independent type 1 (TI-I) stimulation was mimicked by incubation with CpG OND Type B or R848 (InvivoGen, San Diego, USA). TI type 2 (TI-II ...
-
bioRxiv - Cancer Biology 2020Quote: Tumor tissue is collected in Basis medium (Advanced DMEM F12 Serum Free medium (Thermo, 12634010) containing 10mM Hepes (Thermo,15630080), 2mM GlutaMAX supplement (Thermo, 35050061) and 100 μg/ml Primocin (InVivoGen, ant-pm-1)) ...
-
bioRxiv - Developmental Biology 2022Quote: ... dTomato-positive cells were sorted on a BD FACS Aria III using BD FACSDiva 8.0.1 Software using a 100 µm nozzle and cultured on Martigel-coated plates in mTeSR Plus supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and 25 µm Y-27632 ...
-
bioRxiv - Genomics 2022Quote: ... 2 mL STC was added and the protoplasts were mixed with 20 mL liquid MMS containing 0.3 % agar and 200 μg hygromycin mL-1 (InvivoGen, San Diego, CA, USA). The mixture was poured on MMS containing 0.6 % agar and 200 μg hygromycin mL-1 ...
-
bioRxiv - Cancer Biology 2020Quote: Tumor tissue is collected in Basis medium (Advanced DMEM F12 Serum Free medium (Thermo, 12634010) containing 10mM Hepes (Thermo,15630080), 2mM GlutaMAX supplement (Thermo, 35050061) and 100 μg/ml Primocin (InVivoGen, ant-pm-1)) ...
-
bioRxiv - Immunology 2020Quote: ... THP-1-XBlue cells were cultured in complete RPMI medium with the addition of zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA). Prior to stimulation ...
-
bioRxiv - Physiology 2021Quote: ... Duodenal or jejunal segments (∼6 cm) were isolated and placed into falcon tubes containing cold DMEM (high glucose, 4.5 g/l) containing Primocin (Invivogen, cat# ant-pm-1). Intestinal epithelial cells were dissociated using a Gentle Cell Dissociation Reagent (Stemcell Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: Cells were plated on glass-teflon microscope slide (Tekdon, #518plain) and the next day treated with either DMSO or Phleomycin (InvivoGen # ant-ph-1) for 1 hr ...
-
bioRxiv - Immunology 2022Quote: ... 106 UCBMC were stimulated for 16h at 37°C in RPMI 1640 medium supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E. Coli 055:B5; Invivogen, San Diego CA). Brefeldin A (Sigma ...
-
bioRxiv - Developmental Biology 2024Quote: ... H9 hESC cells with high expression of sgRNAs (fused with mCherry) were enriched by G418 selection (1000 μg/ml, InvivoGen, ant-gn-1) for 11 days and flow cytometry sorting ...
-
bioRxiv - Cell Biology 2023Quote: ... We used a variety of inhibitors including Torin 1 (inh-tor1) and Bafilomycin A1 (tlrl-baf1) both purchased from InvivoGen (San Diego, CA). INK128 (S2811) ...
-
bioRxiv - Genomics 2022Quote: ... the tissue was longitudinally cut and subjected to incubation in 3 mM EDTA in ice cold PBS with 1% (v/v) primocin (InvivoGen, San Diego, CA) for 15 min at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... A2780 cells were transduced with pLVX-TetOne-MDM4-Puro and selected for with 1.5 μg/mL puromycin (InvivoGen, cat. no. ant-pr-1). Tight inducibility was confirmed through doxycycline (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... animals were injected intraperitoneally with 1 mg/kg of lipopolysaccharide (LPS endotoxin from E. coli 0111:B4 ultra-pure; InvivoGen, San Diego, CA) dissolved in saline ...
-
bioRxiv - Cell Biology 2024Quote: ... was a gift from Kristen Brennard and stably expressed in RPE1 cells using puromycin selection (10 ug/mL, Invivogen Cat#ant-pr-1). CA-YAP and dCas9-expressing cells were generated using third-generation lentiviral transduction ...
-
bioRxiv - Cancer Biology 2024Quote: ... T47D and MCF7 cells were transduced with the lentiviral particles and stably expressing cells were generated after selection using G418 (InvivoGen ant-gn-1) for two weeks ...
-
bioRxiv - Molecular Biology 2024Quote: ... 293T cells transduced to stably express TIM1 (TIM1-293T) or mock transduced (Mock-293T) were maintained in the medium supplemented with 1 μg/mL puromycin (InvivoGen, Cat# ant-pr).
-
bioRxiv - Cell Biology 2024Quote: ... Stable cell lines generated in this background were cultured with 5 µg/ml of blasticidin and 200 µg/ml of hygromycin B (Invivogen #ant-hg-1). To minimise background expression of the transgene ...
-
bioRxiv - Cell Biology 2024Quote: ... For GSK3 knockout/knockdown cells were generated by transfecting 293T cells with the corresponding lentiCRISPR v2 plasmid with jetPRIME (Polyplus-transfection, Cat# 101000046) and selected with puromycin (InvivoGen ant-pr-1) (1 μg/ml ...
-
bioRxiv - Cancer Biology 2024Quote: ... were cultured in complete RPMI-1640 medium and were transfected with 1 mg/ml cGAS agonist (G3-ended Y-form Short DNA) (InvivoGen, San Diego, USA) and LyoVec™ (InvivoGen ...
-
bioRxiv - Biochemistry 2021Quote: PMA-differentiated THP1 macrophages in 24-well plates were pretreated with fatty acids (2.5 μM and 10 μM) for 30 min prior to stimulation with 4 μg/mL cGAMP (InvivoGen) using Lipofectamine 2000 (Invitrogen) ...
-
bioRxiv - Microbiology 2023Quote: ... 20 µl UV-treated sample was inoculated onto 4×104 cells/ well of HEK-Blue IFNα/β cells (InvivoGen) in 96-well plates ...
-
bioRxiv - Genetics 2023Quote: ... Ganciclovir selection was conducted for 4 days under 10 μM ganciclovir/culture medium (#sud-gcv; InvivoGen, San Diego, CA) after G418 selection or 8 days after single-cell sorting ...
-
bioRxiv - Genetics 2024Quote: ... Selection for blasticidin-expressing infected cells was performed for 3 to 4 days using 3.0 µg/mL blasticidin (InvivoGen), which was added on the 3rd day post-infection.
-
bioRxiv - Immunology 2021Quote: WT and NLRP3-/- iBMDMs were seeded on 150 mm plates at 5×106 cells/plate and on the next day treated with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h. ...
-
bioRxiv - Immunology 2021Quote: ... NLRP3-/- iBMDMs or NLRP3-/- iBMDMs reconstituted with mScarlet-tagged NLRP3 were seeded at 0.5×106 cells/well on a 12-well tissue culture plate and on the next day treated or not with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h or with 1 μg/ml LPS for 2.5 h and additionally with 20 μM MCC950 (Millipore Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... 1% (v/v) nonessential amino acid (Multicell Wisent Inc., St-Bruno, QC, Canada) and 5μg/mL of Plasmocin® (Invivogen, San Diego, CA, USA), to prevent mycoplasma contamination ...
-
bioRxiv - Immunology 2022Quote: ... Cells were stimulated for 24 hours with 1×108 pfu/well of purified Pf4 phage or equivalent volume ΔPf4 preparation and 100 ng/ml LPS (Invivogen, Cat. No. tlrl-eblps). Cells were centrifuged at 300 x g for 5 minutes and supernatant was collected and used for cytokine profiling through Luminex (The Human Immune Monitoring Center ...
-
bioRxiv - Developmental Biology 2024Quote: ... The EGFP-fused lineage barcode was confirmed to exist as a single copy in the genome and to be highly expressed after blasticidin selection (15 g/ml, InvivoGen, no. ant-bl-1) and flow cytometry sorting ...
-
bioRxiv - Immunology 2024Quote: ... with WT or Sulf2+/- antigen-presenting cells (BMDM or DCs) activated with LPS (100 ng/ml LPS, 4 h, InvivoGen). As a source of antigen ...
-
bioRxiv - Immunology 2023Quote: ... the luciferase detection reagent QUANTI-Luc 4 Lucia/Gaussia (Cat. #.: rep-qlc4lg1) and the SEAP detection reagent QUANTI-Blue 4 (Cat. #.: rep-qbs) were purchased from InvivoGen. Cell staining antibodies for the flow cytometry analysis ...
-
bioRxiv - Microbiology 2023Quote: ... 20 μL of cell mixture was transferred to a white opaque 96-well plate and treated with 50 μL of QUANTI-Luc 4 Lucia/Gaussia (InvivoGen) and immediately read using luminescence at 0.1 second read time ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 days after transfection the cells were transferred to 10 cm dish and 4-5 days after transfection cells were selected with 100 μg/ml Hygromycin (InvivoGen). All CRIS-PITCh plasmids are summarized in Table S1.
-
bioRxiv - Immunology 2020Quote: ... NF-kB reporter monocytic cells (THP-1-XBlue) stably expressing a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB were purchased from InvivoGen (InvivoGen, San Diego, CA, USA). - ...
-
bioRxiv - Immunology 2021Quote: ... Cells reconstituted with pLenti CMVie-IRES-BlastR-based constructs were maintained with addition of 5 µg/ml and 1 µg/ml blasticidin (InvivoGen, Cat. no: ant-bl-05) for HEK293T and iBMDMs ...
-
bioRxiv - Cancer Biology 2022Quote: ... Stably transduced cells were either selected by flow cytometry using BD FACSAria III cell sorter or 1μg/ml puromycin (InvivoGen, San Diego, USA, #ant-pr-1).