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Citations for Invivogen :
1101 - 1150 of 1306 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: WT and LRBA-KO HEK293T cells were seeded and treated with 333 nM of Torin 1 (Invivogen) or with 1 nM of VPS34-IN (Biomol ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: ... 10 mM HEPES and 1 mM sodium pyruvate) with or without 100 nM of Bafilomycin A1 (InvivoGen) for 3 h ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were selected with 0.5 ug/ml of puromycin in fresh medium (InvivoGen, cat#ant-pr-1). 48 hours after selection with puromycin ...
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bioRxiv - Immunology 2024Quote: ... Further stimulatory reagents used: 0.25 or 1 µg/ml Resiquimod (R848; InVivoGen, Toulouse, France, dissolved in PBS), 0.5 mM loxoribine (dissolved in dH2O ...
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bioRxiv - Neuroscience 2024Quote: ... ESCs and iPSC lines were supplemented with mTesRTM1 (Stem Cell,85850) with Normacin (Invivogen, ant-nr-1). SH-SY5Y immortalized cell lines were maintained with DMEM-F12 media (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Selection of GFP-positive cells was performed complementing the medium with 1 μg/mL puromycin (InvivoGen/LabForce). GFP-positive bulk cultures were tested for expression and nuclear localization of the transgene-products by immunofluorescence microscopy and western blotting analysis.
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bioRxiv - Immunology 2020Quote: ... the cells were primed for 4 h with 500 ng/mL ultrapure LPS (InvivoGen). The supernatant was discharged and macrophages were infected with trypomastigote forms of T ...
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bioRxiv - Immunology 2024Quote: ... with the STING agonist 5,6-dimethylxanthenone-4-acetic acid (DMXAA) (10 µg/mL, InvivoGen).
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bioRxiv - Pathology 2024Quote: ... Stable cell lines were established by selection with 4 mg/mL of puromycin (InvivoGen) for 2 weeks and maintained in 1 mg/mL of puromycin.
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bioRxiv - Cell Biology 2020Quote: ... on sterilized 5mm diameter metal tissue mounts and fixed in place with sterilized “O-rings.” Tissues were washed thoroughly in sterile PBS (0.01 mol L−1, pH 7.2) supplemented with Primocin (100mg/ml; InvivoGen) and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Immunology 2021Quote: ... On the next day the cells were treated with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h or with 1 μg/ml LPS for 2.5 h and additionally with 20 μM MCC950 (Millipore Sigma ...
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bioRxiv - Immunology 2021Quote: ... MBCs were restimulated in the presence of the TLR7 agonist R848 (1 μg/ml; Invivogen, San Diego, CA) and human IL-2 (10 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... then incubated with 1 µM okadaic acid 30 min before transfer onto transgenic IL-1R reporter cells (Invivogen). After 18 h ...
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bioRxiv - Cell Biology 2022Quote: ... coli as described in Zumthor et al. (Zumthor et al. 2016) Transfectants were selected using Puromycin (final conc. 50 μg ml−1; InvivoGen). Spironucleus vortens and Spironucleus salmonicida were cultured as described before (Paull and Matthews 2001 ...
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bioRxiv - Immunology 2021Quote: Human cell line THP-1 monocytes – ASC speck reporter cells were purchased from Invivogen (#thp-ascgfp, Invivogen, France) and called THP-1-ASC-GFP ...
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bioRxiv - Immunology 2023Quote: ... the PtLp pellet was resuspend in 90 µL of HEPES-KAc buffer with 1 µg mIL-1β (Invivogen) and incubated for 1 h in 30°C ...
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bioRxiv - Immunology 2023Quote: ... Jurkat-Lucia TCR-hPD-1 and Raji-APC-hPD-L1 cells were purchased from InvivoGen (San Diego, CA) and cultured in IMDM media (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Alternating passages of THP1 cells were treated with 200 μg/ml Hygromycin Gold (Invivogen cat. ant-hg-1) as recommended by Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... transfected clones were selected by drug selection with 1 μg/ml of puromycin (InvivoGen, San Diego, CA, USA). Mycoplasma testing was performed using an indirect immunofluorescence test ...
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bioRxiv - Cell Biology 2024Quote: ... and maintained under selection pressure by additionally supplementing the above described medium with 1 µg/mL puromycin (Invivogen). HEK-293T were cultured in DMEM containing 4,5g/L D-glucose ...
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bioRxiv - Cancer Biology 2023Quote: ... Post-transduction selection was conducted over five days with 0.5 µg/mL of puromycin (Invivogen, ant-pr-1) at an MOI of 0.15.
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bioRxiv - Cell Biology 2023Quote: ... co-transfected cells were selected in culture medium supplemented with 3 μg/ml puromycin (Invivogen, ant-pr-1), until selection was complete after about 48 h ...
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bioRxiv - Cancer Biology 2024Quote: ... OSCC cell lines were than infected with the lentiviruses followed by selection using 1 µg/ml puromycin (Invivogen)for 5 days ...
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bioRxiv - Developmental Biology 2022Quote: ... The next day the selection of the cells started with 300 μg/ml G418 (InvivoGen, #ant-gn-1). The selection was done till the colonies were big enough for picking after 7-8 days ...
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bioRxiv - Cell Biology 2022Quote: ... and were selected for ten to fourteen days with a selection medium (contains 1 μg/ml Blasticidin [Invivogen, #ant-bl] ...
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bioRxiv - Cell Biology 2022Quote: ... pUNO1-SpikeV8 carrying delta variant spike and pUNO1-SpikeV11 carrying omicron (B.1.1.529/BA.1 lineage) spike were purchased from InvivoGen (catalogue numbers p1-spike-v8 and p1-spike-v11 respectively).
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bioRxiv - Bioengineering 2022Quote: ... CD8+ T cells and 5×104 NALM-6 cells were plated with 0.5-1 ng/mL blinatumomab (Invivogen) and cytokine ...
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bioRxiv - Immunology 2022Quote: ... Mice were infected intranasally with 3×104 PFU of x31-PmpG-1 virus and boosted intranasally with 1μg PmpG-1303-311 peptide + 2μg CpG (ODN 1826, Invivogen) on days 14 and 28 (75) ...
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bioRxiv - Cancer Biology 2024Quote: ... the old medium was replaced with fresh medium with 400 ug/ml zeocin (InvivoGen, cat#ant-zn-1) for 48 hours to remove non-transfected cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Conditioned medium from the interferon response positive control: HCT116 WT cells transfected with PolyI:C (1 µg/mL, Invivogen) using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Human primary cell cultures were treated empirically for Mycoplasma with 25 mg/mL plasmocin (InvivoGen #ant-mpt-1) after initial isolation for a minimum of two weeks unless used before this for experiments ...
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bioRxiv - Microbiology 2024Quote: ... followed by selection of positive clones in the presence of 10 μg/ml puromycin (ant-pr-1, InvivoGen). All plasmid and siRNA transfections were performed using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... B cell activation was induced using 24-hour incubation with the Tlr9 agonist CpG (Invivogen tlrl-1826-1). In vitro activated lymphocytes were collected and prepared for FISH and IF as previously described22 ...
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bioRxiv - Biochemistry 2023Quote: ... and 50 µL of the working solution of the QUANTI1Luc 4 Lucia/Gaussia reagent (Invivogen) was added ...
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bioRxiv - Immunology 2021Quote: ... 8 x 104 U937 cells per well of a 96 wells plate were exposed to 100 ng.mL-1 of phorbol 12-myristate 13-acetate (PMA; InvivoGen) for 48 h and primed with LPS at 1 μg.mL-1 for 3 h ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 µg of pcDNA5 construct were co-transfected with 1.5 µg Flippase expression vector (pOG44) and cells were selected with 100 µg ml-1 Hygromycin (InvivoGen). All cell lines generated and plasmids used in this study are listed in Supplementary Table 1.
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bioRxiv - Immunology 2021Quote: ... were transduced 3 times with lentivirus containing supernatant and selected with 1 μg/ml Puromycin (Invivogen, San Diego, USA). Surviving cells were expanded and referred to as HEK293 luciferase reporter cells.
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bioRxiv - Immunology 2021Quote: ... cells were pretreated for 1 hour with Chloroquine or CU-CPT9a or stimulated with TLR agonists (all from Invivogen).
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bioRxiv - Microbiology 2021Quote: ... Two days post-transfection the media was replaced with DMEM containing 0.5% fetal bovine serum and NOD1 or NOD2 agonist [1 μg/ml C12-iE-DAP (Invivogen)+ 10 ng/ml human interferon gamma (AbD serotec ...
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bioRxiv - Microbiology 2020Quote: ... DNA ratio of 3:1 according to manufacturer’s instructions or with Poly(I:C) (LMW)/LyoVec™ (tlrl-picwlv, InvivoGen). The treatment was administered as indicated ...
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bioRxiv - Cancer Biology 2021Quote: ... LPS-EK (1 μg/mL) and ODN2006 (10 μg/mL) were purchased from InvivoGen (tlrl-kit1hw and tlrl-pslta). Calf thymus DNA was purchased from Sigma (D1501) ...
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bioRxiv - Immunology 2022Quote: ... 2×104 DCs were co-cultured for 3 days with 5×104 FACS sorted naïve OT-II CD4+ T cells in the presence of 1 µg/ml OVA peptide (OVA323-339, Invivogen) and 0.25 ng/ml TGFβ (recombinant mouse TGF-b1 ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa EGFP-CHIP and HEK EGFP-CHIP cells were supplemented with blasticidin (10 µg/ml) (ant-bl-1, Invivogen) and hygromycin B (50 µg/ml ...
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bioRxiv - Microbiology 2020Quote: ... IFN-β promoter activity was induced by transfecting 250 ng of poly I:C (average size 0.2-1 kb) (InvivoGen) during 16 h ...
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bioRxiv - Cell Biology 2021Quote: Endogenously tagged cell lines were maintained in HMI-9 supplemented with 20 % (v/v) FCS at 37 °C in 5% C02 and maintained in 10 µg.mL-1 Blasticidin (InvivoGen). Parasite density was monitored using a haemocytometer.
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bioRxiv - Immunology 2022Quote: ... d6-monocytes were stimulated with either 3pRNA as described above or 1 μg/ml P3CSK (InvivoGen, San Diego, USA) 5h prior to ATP challenge ...
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bioRxiv - Microbiology 2021Quote: ... Long-term passaging of landing pad cells utilized D10-dox with 20 μg/mL blasticidin (InvivoGen, ANT-BL-1) to remove cells with silenced landing pad loci.
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The selection was started 48 hours after transfection using growth media supplemented with 500µg/ml Hygromycin Gold (ant-hg-1, Invivogen). Selection media was changed every 3-4 days until confluent colonies of stable transgenic cells had formed and cells were ready to be split.
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... total splenocytes isolated from OT-II mouse spleens were cultured with OVA 323-339 peptide (1 mg/ml, InvivoGen) and recombinant murine IL-2 (20 ng/ml ...
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bioRxiv - Immunology 2021Quote: Enriched human memory B cells were cultured at 0.2 x 106 cells per ml in complete RPMI supplemented with 1 mg/ml R848 (Invivogen), 1 mg/ml soluble CD40L (Fitzgerald) ...