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Citations for Invivogen :
1201 - 1250 of 1268 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 24-well or 96-well plates at a concentration of 5×105 cells/mL in RPMI 1640 supplemented with 20% fetal calf serum and 1X primocin (Invivogen).
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bioRxiv - Biochemistry 2024Quote: ... The identities of cell lines were confirmed by chromosomal marker analysis and tested negative for mycoplasma using a MycoStrip (InvivoGen).
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bioRxiv - Immunology 2021Quote: ... HEK-Blue TLR3 and HEK-Blue TLR4 reporter cells (Cat. no.# hkb-htlr2, hkb-htlr3 and hkb-htlr4) were purchased from InvivoGen (USA). Human monocytic cells ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μL of the supernatant or an IFNα/β standard containing purified IFNα and IFNβ were added to 180 μL HEK-Blue cells (Invivogen) (50,000 cells ...
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bioRxiv - Developmental Biology 2020Quote: ... The clonal Dia-C6 cells were used for lenti-sgRNA transduction followed by 20 µg/mL blasticidin (InvivoGen, ant-bl-1) selection and fluorescence cell sorting for mNeonGreen or mScarlet (see Plasmids ...
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bioRxiv - Immunology 2019Quote: ... cells were incubated for additional 5h with 50 ng/ml Phorbol-12- myristate-13-acetate (PMA) (InvivoGen, San Diego, CA, USA) + 1 µg/ml Ionomycin (MP Biomedicals ...
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bioRxiv - Physiology 2021Quote: ... transfection: Cells with 60-80% confluency were transfected with synthetic double-stranded RNA poly(I:C) (high molecular weight, InvivoGen #tlrl-pic) using lipofectamine 2000 following the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... A459/KZFP cells were cultured in F-12K medium with 1.0 μg/ml puromycin (Invivogen, San Diego, CA; #ant-pr-1). An A549 cell line stably expressing Cas9 (A549/Cas9 cells ...
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bioRxiv - Cell Biology 2022Quote: ... 24 and a Blasticidin resistance gene used for the initial selection of infected cells (10 µg/ml Blasticidin, Invivogen, ant- bl). MEF cells were cultivated in DMEM (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: B-ALL cells were infected with Cas9-2A-blast lentivirus followed by one-week selection with 5-15 µg/mL blasticidin (InvivoGen, CA). Cells were amplified and tested for Cas9 activity using the reporter assay described in Tzelepis et al.14 Pools with >75% Cas9 activity were used going forward ...
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bioRxiv - Microbiology 2022Quote: ... and TLR9 and TLR7 activation was determined by measuring secreted embryonic alkaline phosphatase (SEAP) reporter activity in cell culture supernatants by QuantiBlue™ reagent (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Microbiology 2022Quote: ... RIG-I stimulation was assessed by analyzing Lucia luciferase reporter gene expression in 20 µl cell culture supernatants using QUANTI-Luc™ (Invivogen). Data are expressed as the normalized fold change over mock infected cells ...
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bioRxiv - Microbiology 2021Quote: ... 50 µL of each cell supernatant were transferred into separate plates and added 50 µL of quantiblue reagent™ following manufacturer’s instructions (InvivoGen Inc.). Afterwards ...
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bioRxiv - Systems Biology 2021Quote: ... Different innate immune stimuli were applied to cell medium at 10% culture volume as follows: High molecular weight (HMW) poly(I:C) (Invivogen #tlrl-pic) at a concentration of 1mg/mL for 2 h (final concentration in the well 100 ug/mL ...
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bioRxiv - Systems Biology 2021Quote: ... 20 μL of the cell media supernatant was added to 50 μL of QUANTI-Luc™ assay solution (Invivogen #rep-qlc1) in a white 96-well microplate (Greiner ...
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bioRxiv - Cell Biology 2021Quote: ... Double knockout cells were selected by using a combination of 1μg/ml Puromycin and 6μg/ml Blasticidin (Invivogen, #ant-bl-10p) for 1 week.
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bioRxiv - Microbiology 2021Quote: ... HEK293-FT_hACE2 and A549_hACE2 cells were grown in their correspondent medium with 250-500 μg/ml of blasticidin (Invivogen # ant-bl-1). All cell media were supplemented with 8% (v/v ...
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bioRxiv - Immunology 2022Quote: ... THP-1 Dual cells were grown in the presence of respective selection agents [100 mg/mL zeocin (InvivoGen, #ant-zn-1) and 10 mg/mL blasticidin (InvivoGen ...
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bioRxiv - Microbiology 2022Quote: Selection of cells transfected with in vitro RNA transcripts produced from replicon constructs was initiated by addition of puromycin (InvivoGen, UK) to the culture media at 24 - 48 hours post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... 20 µl of cell supernatant were transferred to new plates and supplemented with freshly made 180 µl Quanti-Blue solution (Invivogen, France). After 180 minutes of incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... Matrigel/cell mix was incubated for 15 min at 37C to allow for solidification and base media with Primocin® (InvivoGen) was added to top and bottom chambers of transwell ...
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bioRxiv - Immunology 2023Quote: The RAW-Blue ISG macrophage cell line stably transfected with an ISGF3-dependent reporter construct was cultured and maintained according to the manufacturer’s instructions (RAW-Blue ISG cells, Invivogen raw-isg). ISGF3 reporter activity was determined from cell culture supernatants using QUANTI-Blue detection reagents according to the manufacturer’s instructions (Invivogen).
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bioRxiv - Microbiology 2023Quote: The ADCC reporter assay was performed by using Jurkat-Lucia NFAT-CD16 cells (human FcγRIII, V158) (Invivogen, Cat# jktl-nfat-cd16) as effector cells according to the manufacturer’s instruction with modifications ...
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bioRxiv - Immunology 2023Quote: The inhibitory effect of APB-R3 on IL-18 signal transduction was tested using the HEK-BlueTM IL-18 reporter cell assay system (InvivoGen, US). APB-R3 and recombinant IL-18BP were appropriately diluted ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated for 6 h with the indicated concentration of either High Molecular Weight (HMW) Poly(I:C) (InvivoGen, tlrl-pic) or Poly(I:C ...
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bioRxiv - Immunology 2024Quote: ... differentiated for seven days with 50ng/mL M-CSF) and adherent peritoneal exudate cells (PECs) incubated with and without 200ng/mL lipopolysaccharide (LPS, InvivoGen, #tlrlpb5lps) for 6 hours (Fig S1D) ...
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bioRxiv - Immunology 2023Quote: ... All cell lines were negative for mycoplasma contamination as confirmed using a MycoStrip™ - Mycoplasma Detection Kit (InvivoGen rep-mys-10). FBS was heat inactivated prior to use at 56°C for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: All materials were verified to be free of endotoxins using the HEK-Blue mTLR4 reporter cell line (InvivoGen, San Diego, CA) using the protocol provided by the supplier ...
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bioRxiv - Molecular Biology 2020Quote: ... H1299 cells were transduced by the lentivirus vectors and selected with DMEM containing 5 µg/ml puromycin (InvivoGen, San Diego, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... 20 μl of supernatants from the HEK-Blue™ or THP1-Blue™ cells were mixed with 180 μl of QuantiBlue reagent (InvivoGen) and optical density (O.D. ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... hDaxx or ATRX were maintained in HFF cell culture medium additionally supplemented with 5 μg/ml puromycin (InvivoGen, San Diego, CA, USA). HFF expressing FLAG-tagged PML isoforms or mCherry-tagged PML were cultured in the presence of 500 μg/ml geneticin (InvivoGen ...
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bioRxiv - Bioengineering 2020Quote: ... 20 µL of cell culture supernatant was then withdrawn and analyzed for alkaline phosphatase content via change in Quanti-Blue (Invivogen, rep-qbs) absorbance at 620 nm (Spectramax id3 plate reader).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were treated with 400 µM FFA and 3 µM HSG4112 in the presence or absence of bafilomycin A1 (Invivogen, tlrl-baf1). Cellular localization of LC3B was observed using a Carl Zeiss Confocal LSM710 Meta microscope and the images were processed with the software supplied by the manufacturer (Carl Zeiss ...
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bioRxiv - Cell Biology 2020Quote: ... During this incubation cells were resuspended in DMEM supplemented with 10% (vol/vol) FBS and 1x Normocin (InvivoGen, San Diego, CA, USA) and 6,000 cells were dispensed onto each well loaded with medium for a final siRNA concentration of 20 nM ...
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bioRxiv - Cancer Biology 2020Quote: ... ≈80% confluent adherent cells were washed twice in 1× PBS and subsequently pulsed with puromycin-containing media (InvivoGen, 10 μg mL−1) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... A549-Cas9 cells were transduced with a lentivirus containing hACE2 and subjected to Zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA) at 72 h after transduction ...
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bioRxiv - Bioengineering 2023Quote: ... 2×105 PBMCs and 4×104 autologous CD8+ T cells were incubated with either supernatants from engineered PCs or media containing recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) or anti-CD33 x anti-CD3 bispecific (AMG 330 ...
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bioRxiv - Bioengineering 2023Quote: ... 5×103 control cells and 5×104 CD8+ T cells were incubated with either various dilutions of supernatants from genome-engineered cells or media containing various concentrations of recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) for 48 hours (Figure 1F-H & 3G-I) ...
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bioRxiv - Biochemistry 2023Quote: ... Selection and maintenance of cells that underwent recombination started 24 h later by including hygromycin (50 µg/ml, Invivogen ant-hg-5) in the culture media ...
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bioRxiv - Neuroscience 2023Quote: ... To assess autophagic function, cells were treated with Bafilomycin A1 (BafA1, 200 nM, Enzo, BML-CM110-0100) and/or Torin1 (200 nM, Invivogen, inh-tor1). All compounds were mixed with culture medium and placed onto plated cells for 4 h before extraction.
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bioRxiv - Microbiology 2023Quote: ... The plasmid was transfected into Huh7 cells using TransIT LT-1 transfection reagent and after selection with puromycin dihydrochloride (Invivogen, CA, USA), a single colony was isolated using cloning cylinders (Merck ...
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bioRxiv - Bioengineering 2024Quote: All materials for in vivo use were tested for endotoxin using the HEK-Blue mTLR4 reporter cell line (InvivoGen, San Diego, CA) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then sorted for GFP-positive to enrich for CROP-seq-vector-positive cells and plated on matrigel-coated plates in mTesr1supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and Y-27632 (final concentration 5µM) ...
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bioRxiv - Molecular Biology 2021Quote: ... Parent Flp-In™ T-REx™-293 cells were additionally supplemented with 15 μg/ml blasticidin (InvivoGen, cat n°ant-bl-1) and 100 μg/ml zeocin for cultivation ...
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bioRxiv - Developmental Biology 2020Quote: ... and cells were re-plated to a 75 cm2 flask in culture medium supplemented with 20 µg/mL blasticidin (InvivoGen, ant-bl-1) to begin the antibiotic selection.
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bioRxiv - Neuroscience 2019Quote: ... Tet-inducible EGFP-RBM45 stable cell lines (see below) were maintained in the presence of 50μg/ml Zeocin (Invivogen, San Diego, CA, USA). The expression of EGFP-RBM45 was induced with 1 μg/ml doxycycline hyclate (Sigma ...
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bioRxiv - Immunology 2020Quote: ... T cell independent type 1 (TI-I) stimulation was mimicked by incubation with CpG OND Type B or R848 (InvivoGen, San Diego, USA). TI type 2 (TI-II ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were selected by at least one-week treatment with 150 ug/ml G418 or 10 μg/mL blasticidin (InvivoGen, San Diego, CA). Bulk populations of selected cells were used in this study ...
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bioRxiv - Immunology 2020Quote: ... THP-1-XBlue cells were cultured in complete RPMI medium with the addition of zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA). Prior to stimulation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... we used engineered HEK293 cell lines (HEK-Blue) that stably express either mouse TLR9 (mTLR9) and an NF-κB reporter gene (Invivogen, San Diego, CA). In brief ...