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Citations for Invivogen :
1151 - 1200 of 1268 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... at a concentration of 1×106 T cells per well in RPMI medium supplemented with Primocin (100 µg/ml; Invivogen), 10 % charcoal stripped FBS (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2 and A549-S cell lines were selected using 10 μg/ml puromycin (InvivoGen, catalog no. ant-pr-1) 4 days post-transduction ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines have been routinely tested for mycoplasma contamination (Mycoplasma contamination detection kit; rep-pt1; InvivoGen - San Diego - CA).
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bioRxiv - Pathology 2019Quote: ... Confluent mIMCD-3 cells were serum deprived for 16 hours and then stimulated with 1µg/mL LPS (Invivogen, tlrl-3pelps). RNAs and proteins were extracted 24 hours after stimulation.
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bioRxiv - Cancer Biology 2019Quote: Retrovirus was generated by plating PlatE cells for 1 week in complete DMEM with the addition of selection antibiotics including 1 µg/mL puromycin (InvivoGen) and 10 µg/mL blasticidin (InvivoGen) ...
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bioRxiv - Immunology 2019Quote: moDCs and THP-1 cells were left unstimulated or stimulated with 10 µg/ml MDP or 1 µg/ml PAM3CSK4 (Invivogen) or both at the indicated time points ...
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bioRxiv - Immunology 2019Quote: ... BMDMs were seeded at a density of 2 × 105 cells/well in 48-well plates and pre-stimulated with 500 ng ml-1 of LPS (tlrl-peklps; InvivoGen) for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... tricornutum cell lines were supplemented with 50 or 100 μg/mL phleomycin (InvivoGen, San Diego, CA, Catalog #ant-ph-10p).
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bioRxiv - Microbiology 2020Quote: ... Recombinant human antibodies were expressed in HEK-293T cells following transient transfection with pairs of the IgG1 heavy and light chain expression plasmids according to protocols from Invivogen. Recombinant antibodies were purified using Protein A sepharose (IBA ...
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bioRxiv - Biochemistry 2021Quote: ... Stable MCF10A cells expressing these proteins were generated from MCF10A ARPIN −/− cells 22 transfected as described above and selected with 10 μg/ml Blasticidin (InvivoGen). Individual clones were picked with cloning rings.
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bioRxiv - Microbiology 2020Quote: ... at 37 °C in 5 % CO2 and differentiated into macrophage-like phenotype cells by adding 100 nM of phorbol 12-myristate 13-acetate (PMA; InvivoGen) two days prior infection by ZIKV.
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bioRxiv - Microbiology 2020Quote: ... or 7.7G6 mAb were produced in HEK-293T cells following transfection with pairs of the IgG1 heavy and light chain expression plasmids according to protocols from InvivoGen. Human antibodies were purified from cell culture supernatants using Protein-A affinity chromatography ...
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bioRxiv - Immunology 2020Quote: ... Transfected HEK-Blue-KD-TLR5 cells were then gently flushed in PBS and resuspended in HEK-Blue Detection Media (Invivogen) at 2.8 × 105 cells/mL ...
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bioRxiv - Immunology 2020Quote: Total spleen cells were cultured at a density of 1 × 106 cells/mL in RPMI 1640 supplemented with 10% fetal calf serum (FCS) with 5 ng/mL LPS (Invivogen). For 4-hydroxytamoxifen (OHTAM ...
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bioRxiv - Immunology 2021Quote: ... inhibitors were added to the T cells cultured in 24-well stimulation plates at the following concentrations 6-8 hours prior to electroporation: ODN A151 (Invivogen): 5 µM ...
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bioRxiv - Cancer Biology 2020Quote: ... transient transfection of HEK-293FT cells with packaging and lentiviral vector plasmids were performed using LENTI-Smart INT kit (InvivoGen) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were seeded at 1 × 105 cells/well in 96-well U-bottom plates in media containing 500 ng/mL Pam3CSK4 (Invivogen) for 4-6 hours then treated with either Val-boroPro (10 μM ...
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bioRxiv - Immunology 2022Quote: Splenic B cells were isolated from WT and cKO mice and cultured in presence of LPS (InvivoGen, San Diego, CA) 5 µg/ml or 1 µg/ml for 2 ...
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bioRxiv - Biochemistry 2022Quote: ... hACE2- and S-expressing cells were maintained in hygromycin medium (complete growth medium supplemented with 100 μg/mL hygromycin B [Invivogen]) and puromycin medium (complete growth medium supplemented with 1 μg/mL puromycin [Invivogen]) ...
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bioRxiv - Immunology 2022Quote: ... Jurkat-Lucia™ NFAT-CD16 cells and Jurkat-Lucia™ NFAT-CD32 were obtained by InvivoGen (San Diego, CA, USA) cat ...
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bioRxiv - Immunology 2022Quote: ... shControl and shLRBA HeLa cells were seeded at 0.3*106 cells in 6 well-plates followed by treatment for 16 h or 24 h with 5 μM of MG132 (InvivoGen) alone or in combination with 100 nM of Bafilomycin A1 for 3 h ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were seeded as 0.3*106 cells in 6-wells plate followed by treatment for 4 h with 333 nM of Torin 1 (InvivoGen) alone or in combination with 200 nM of Bafilomycin A1 for 1 h.
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bioRxiv - Immunology 2022Quote: PBMCs were differentiated into memory B cells by incubating them with 500 ng/mL R848 (Invivogen, San Diego, CA, USA) and 5 ng/mL rIL-2 (R&D ...
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... Lucia and Cypridina luciferase activity was quantified by bioluminescence from aliquots of the cell supernatant (BioLux Cypridina Luciferase assay kit, New England Biolabs; Quanti-Luc, InvivoGen). Reference plasmid-normalized luciferase activity was from the average of three independent transfections ...
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bioRxiv - Neuroscience 2022Quote: ... some cells were activated with LPS (100 ng per mL, triple-purified from E. coli strain O55:B5, InvivoGen, Inc.) or IFNγ (20 ng per mL) ...
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bioRxiv - Microbiology 2022Quote: VeroE6/TMPRSS2 (JCRB 1819) cells (Matsuyama et al., 2020) were propagated in the presence of 1 mg/ml geneticin (G418; Invivogen) and 5 μg/ml plasmocin prophylactic (Invivogen ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells stably expressing human TMPRSS2 were generated by lentiviral transduction followed by selection with blasticidin (40 μg/mL; Invivogen). SARS-CoV-2 strains hCoV-19/USA-WA1/2020 (NR-52281) ...
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bioRxiv - Molecular Biology 2022Quote: ... The wild-type PCR product and the digested pCI vector backbone were ligated and transformed into GT115 competent cells (InvivoGen). To introduce the ABCA4 c.5461-10T>C mutation ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were grown until confluency and reseeded into 6-well dishes prior to addition of 1 µg/ml puromycin (InvivoGen) and 5 µg/ml blasticidin (InvivoGen) ...
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bioRxiv - Bioengineering 2023Quote: ... and 1000 ng/mL) was transfected into the cells using LyoVec cationic lipid-based transfection agent (Catalog Code, lyec-12, InvivoGen). Briefly ...
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bioRxiv - Systems Biology 2023Quote: ... Transfected cells were incubated for 24 hours before transfection media was replaced with media containing 10 ug/ml blasticidin (Invivogen). Cells were incubated for 30 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Jurkat cells expressing the TCR reporter construct were maintained in culture with 10 µg/ml blasticidin (Invivogen ant-bl-1). For TCR expression ...
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bioRxiv - Bioengineering 2023Quote: ... 50 μL cell supernatant was collected from each sample and added to 150 μL of QUANTI-Blue SEAP detection medium (InvivoGen) and incubated for 2 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... 20 µl of media from the THP-1 Dual cells was incubated with 180 µl Quanti-Blue (InvivoGen, # rep-qbs) and incubated for 4 hours at 37°C before quantification at OD635 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Compounds were incubated with the reporter cells for 48 h before 10 μL of 0.5 × QUANTI-Luc (InvivoGen, rep-qlc2) was added to each well ...
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bioRxiv - Immunology 2023Quote: ... FreeStyleTM 293-F cells were co-transfected with the specified M2e-MAbs heavy and light chain variable region plasmids (InvivoGen) using Polyethylenimine (PEI ...
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bioRxiv - Genomics 2022Quote: ... while material that was collected with washing of the cell strainer surface with ice cold PBS with 1% (v/v) primocin (InvivoGen) was referred to as jejunal epithelial cell fraction two (IEC-2) ...
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bioRxiv - Immunology 2023Quote: ... Ultrapure LPS for cell culture experiments (lipopolysaccharide from Escherichia coli 0111:B4) was purchased from Invivogen (San Diego, CA, USA).
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bioRxiv - Immunology 2023Quote: Bioactive functional type I IFN was quantified in supernatants using the reporter cell line HEK-Blue IFN-α/β (Invivogen) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: Hela-S3 cells were purchased from ATCC and cultured in DMEM supplemented with 10 % heat inactivated FBS without antibiotics and incubated at 37 °C and 5 % CO2 Cells were tested regularly for mycoplasma with the PlasmoTest kit (InvivoGen) and are mycoplasma negative ...
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bioRxiv - Immunology 2023Quote: ... cells were pretreated with the caspase-1 inhibitor YVAD (Enzo) (1 μM) or with the NLRP3 inhibitor MCC-950 (Invivogen) (10 μM ...
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bioRxiv - Immunology 2024Quote: ... with WT or Sulf2+/- antigen-presenting cells (BMDM or DCs) activated with LPS (100 ng/ml LPS, 4 h, InvivoGen). As a source of antigen ...
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bioRxiv - Microbiology 2023Quote: ... S10-3 ITGB1 KO cells were transduced with pWPI-ITGB1 and selected in cDMEM supplemented with 400 μg/ml G418 (Invivogen). S10-3 WT cells were transduced with pTRIPZ-Rab5/7/11-GFP or EGFP-LAMP1 and selected in cDMEM supplemented with 2 μg/ml puromycin (Invivogen).
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bioRxiv - Molecular Biology 2023Quote: ... Three days post-transfection cells were sub-cultured into media supplemented with puromycin (0.8 µg/mL; Invivogen, ANT-PR-1). After six days of selection ...
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bioRxiv - Immunology 2024Quote: Assessment of TLR4 and TLR5 activity was implemented using human embryonic kidney (HEK)-Blue-mTLR4 and HEK-Blue-mTLR5 cells respectively (Invivogen) using a protocol as previously described (Allen et al. ...
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bioRxiv - Immunology 2024Quote: ... 2cz-Tag-it co-culture with RAW 264.7 cells activated for 6 hours with 1.5 ug/ml Ultrapure LPS from E.coli O111:B4 (InvivoGen, Cat#tlrl-3pepls) and 2cz cultured with 50 ng/ml of its strong agonist peptide SIYRYYGL (GenScript ...
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bioRxiv - Microbiology 2024Quote: ... A positive control for TNF-α or IL-1β release was also prepared by challenging cells with 1 μg/ml lipopolysaccharide (Escherichia coli 0111:B4; InvivoGen) for an equivalent time ...
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bioRxiv - Cancer Biology 2024Quote: ... 24-well or 96-well plates at a concentration of 5×105 cells/mL in RPMI 1640 supplemented with 20% fetal calf serum and 1X primocin (Invivogen).
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bioRxiv - Genomics 2023Quote: ... Cells were then imaged in the imaging buffer (0.5 μg/ml DAPI, 10 μg/ml Fungin (InvivoGen ant-fn-1) in PBS ...
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bioRxiv - Genetics 2024Quote: ... the pooled mixture of cells were expanded and subsequently maintained in D10-dox containing 1 μg/mL puromycin (InvivoGen, ANTPR).