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Citations for Invivogen :
1101 - 1150 of 1167 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... All cell lines were negative for mycoplasma contamination as confirmed using a MycoStrip™ - Mycoplasma Detection Kit (InvivoGen rep-mys-10). FBS was heat inactivated prior to use at 56°C for 30 minutes ...
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bioRxiv - Immunology 2023Quote: The RAW-Blue ISG macrophage cell line stably transfected with an ISGF3-dependent reporter construct was cultured and maintained according to the manufacturer’s instructions (RAW-Blue ISG cells, Invivogen raw-isg). ISGF3 reporter activity was determined from cell culture supernatants using QUANTI-Blue detection reagents according to the manufacturer’s instructions (Invivogen).
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bioRxiv - Microbiology 2023Quote: ... Recombinant IgA1 produced in ExpiCHO-S cells as described above was purified using protein M agarose beads (Cat# gel-pdm-2, InvivoGen US) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The ADCC reporter assay was performed by using Jurkat-Lucia NFAT-CD16 cells (human FcγRIII, V158) (Invivogen, Cat# jktl-nfat-cd16) as effector cells according to the manufacturer’s instruction with modifications ...
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bioRxiv - Immunology 2023Quote: The inhibitory effect of APB-R3 on IL-18 signal transduction was tested using the HEK-BlueTM IL-18 reporter cell assay system (InvivoGen, US). APB-R3 and recombinant IL-18BP were appropriately diluted ...
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bioRxiv - Bioengineering 2023Quote: ... or ss-ppp-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (Catalog Code, lyec-12, InvivoGen), following the manufacturer’ ss instructions ...
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bioRxiv - Bioengineering 2023Quote: ... or ss-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (InvivoGen, Catalog Code. lyec-12, InvivoGen) following the manufacturer’ ss instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated for 6 h with the indicated concentration of either High Molecular Weight (HMW) Poly(I:C) (InvivoGen, tlrl-pic) or Poly(I:C ...
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bioRxiv - Molecular Biology 2024Quote: THP1-DualTM KI STING cells harboring either the WT allele or V155M point mutation were acquired from Invivogen (cat# thpd-m155). Both cell lines were maintained and passaged according to manufacturer’s recommendation ...
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bioRxiv - Immunology 2024Quote: ... cells in the wells designated for “stimulation” were resuspended in a combination of Resiquimod (R848) (4µg/mL; Invivogen Cat# tlrl-r848) and Polyinosinic:polycytidylic acid (Poly IC ...
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bioRxiv - Microbiology 2024Quote: Levels of AhR ligands were measured in milk samples using HT-29-Lucia™ AhR reporter cells (Invivogen, San Diego, California). Briefly ...
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bioRxiv - Microbiology 2024Quote: Cells were seeded at a density of 1.5×105 cells/well for 24 hours followed by transfection with 10 ug of poly(I:C) chemically labeled with rhodamine (Invivogen, #tlrl-picr) for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... IFN-α2 levels were independently quantified from the same cell supernatants with the LumiKine Xpress hIFNa 2.0 ELISA (InvivoGen luex-hifnav2) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... H1299 cells were transduced by the lentivirus vectors and selected with DMEM containing 5 µg/ml puromycin (InvivoGen, San Diego, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... 20 μl of supernatants from the HEK-Blue™ or THP1-Blue™ cells were mixed with 180 μl of QuantiBlue reagent (InvivoGen) and optical density (O.D. ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... hDaxx or ATRX were maintained in HFF cell culture medium additionally supplemented with 5 μg/ml puromycin (InvivoGen, San Diego, CA, USA). HFF expressing FLAG-tagged PML isoforms or mCherry-tagged PML were cultured in the presence of 500 μg/ml geneticin (InvivoGen ...
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bioRxiv - Bioengineering 2020Quote: ... 20 µL of cell culture supernatant was then withdrawn and analyzed for alkaline phosphatase content via change in Quanti-Blue (Invivogen, rep-qbs) absorbance at 620 nm (Spectramax id3 plate reader).
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bioRxiv - Microbiology 2022Quote: ... 293-ACE2 cells were maintained in DMEM supplemented with 10% fetal bovine serum and 200 µg/mL G418 (Invivogen #ant-gn-2). HEK-Blue 293 hACE2-TMPRSS2 cells (Invivogen #hkb-hace2tpsa ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were treated with 400 µM FFA and 3 µM HSG4112 in the presence or absence of bafilomycin A1 (Invivogen, tlrl-baf1). Cellular localization of LC3B was observed using a Carl Zeiss Confocal LSM710 Meta microscope and the images were processed with the software supplied by the manufacturer (Carl Zeiss ...
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bioRxiv - Cell Biology 2020Quote: ... During this incubation cells were resuspended in DMEM supplemented with 10% (vol/vol) FBS and 1x Normocin (InvivoGen, San Diego, CA, USA) and 6,000 cells were dispensed onto each well loaded with medium for a final siRNA concentration of 20 nM ...
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bioRxiv - Cancer Biology 2020Quote: ... ≈80% confluent adherent cells were washed twice in 1× PBS and subsequently pulsed with puromycin-containing media (InvivoGen, 10 μg mL−1) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... A549-Cas9 cells were transduced with a lentivirus containing hACE2 and subjected to Zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA) at 72 h after transduction ...
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bioRxiv - Bioengineering 2024Quote: All materials for in vivo use were tested for endotoxin using the HEK-Blue mTLR4 reporter cell line (InvivoGen, San Diego, CA) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... 2×105 PBMCs and 4×104 autologous CD8+ T cells were incubated with either supernatants from engineered PCs or media containing recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) or anti-CD33 x anti-CD3 bispecific (AMG 330 ...
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bioRxiv - Bioengineering 2023Quote: ... 5×103 control cells and 5×104 CD8+ T cells were incubated with either various dilutions of supernatants from genome-engineered cells or media containing various concentrations of recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) for 48 hours (Figure 1F-H & 3G-I) ...
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bioRxiv - Immunology 2023Quote: ... Guide-RNAs were stably transduced in SpCas9-expressing DC 2.4 cells using the LentiGuide-Neo vector and selected with 500 ug/mL of G418 (InvivoGen #108321-42-2). LentiGuide-Neo was a gift from Caroline Goujon (Addgene plasmid # 139449 ...
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bioRxiv - Biochemistry 2023Quote: ... Selection and maintenance of cells that underwent recombination started 24 h later by including hygromycin (50 µg/ml, Invivogen ant-hg-5) in the culture media ...
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bioRxiv - Neuroscience 2023Quote: ... To assess autophagic function, cells were treated with Bafilomycin A1 (BafA1, 200 nM, Enzo, BML-CM110-0100) and/or Torin1 (200 nM, Invivogen, inh-tor1). All compounds were mixed with culture medium and placed onto plated cells for 4 h before extraction.
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then sorted for GFP-positive to enrich for CROP-seq-vector-positive cells and plated on matrigel-coated plates in mTesr1supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and Y-27632 (final concentration 5µM) ...
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bioRxiv - Molecular Biology 2021Quote: ... Parent Flp-In™ T-REx™-293 cells were additionally supplemented with 15 μg/ml blasticidin (InvivoGen, cat n°ant-bl-1) and 100 μg/ml zeocin for cultivation ...
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bioRxiv - Developmental Biology 2020Quote: ... and cells were re-plated to a 75 cm2 flask in culture medium supplemented with 20 µg/mL blasticidin (InvivoGen, ant-bl-1) to begin the antibiotic selection.
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bioRxiv - Immunology 2020Quote: ... T cell independent type 1 (TI-I) stimulation was mimicked by incubation with CpG OND Type B or R848 (InvivoGen, San Diego, USA). TI type 2 (TI-II ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were selected by at least one-week treatment with 150 ug/ml G418 or 10 μg/mL blasticidin (InvivoGen, San Diego, CA). Bulk populations of selected cells were used in this study ...
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bioRxiv - Immunology 2020Quote: ... THP-1-XBlue cells were cultured in complete RPMI medium with the addition of zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA). Prior to stimulation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... we used engineered HEK293 cell lines (HEK-Blue) that stably express either mouse TLR9 (mTLR9) and an NF-κB reporter gene (Invivogen, San Diego, CA). In brief ...
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bioRxiv - Immunology 2020Quote: ... Immune cell stimulations were performed in the absence (Control) or presence of 0,1 μg/ml of extrapure Lipopolysacharide (Invivogen LPS, Cat. tlrl-3pelps), 10 μg/ml Poly I:C (Invivogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The final product was verified with Sanger sequencing from both directions and incorporated into cells using PEI transfection and selection with G418 (InvivoGen, ant-gn-5).
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bioRxiv - Synthetic Biology 2022Quote: ... 20 μL of supernatants from the reporter cells (HEK-Blue IFN-α/β or HEK-Blue IFN-λ) were added to 180 μL of Quanti-blue substrate (InvivoGen) and incubated at 37°C for 1-2 hours ...
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bioRxiv - Immunology 2022Quote: ... Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen, San Diego, US) added at alternative passages ...
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bioRxiv - Developmental Biology 2024Quote: ... H9 hESC cells with high expression of sgRNAs (fused with mCherry) were enriched by G418 selection (1000 μg/ml, InvivoGen, ant-gn-1) for 11 days and flow cytometry sorting ...
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bioRxiv - Immunology 2024Quote: Human TLR4 reporter cell line HEK-TLR4 cells containing a nuclear factor κ-light-chain-enhancer of activated B cells (NFκB)-inducible secreted embryonic alkaline phosphate (SEAP) reporter (InvivoGen; San Diego, CA) were used to assess the effect of resistin on TLR4 activation as previously reported15 ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were routinely confirmed to be negative for mycoplasma infection every 3–4 months using PlasmoTest mycoplasma detection kit (InvivoGen, catalog # rep-pt1). No other authentication assay was performed.
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bioRxiv - Cancer Biology 2023Quote: ... A2780 cells were transduced with pLVX-TetOne-MDM4-Puro and selected for with 1.5 μg/mL puromycin (InvivoGen, cat. no. ant-pr-1). Tight inducibility was confirmed through doxycycline (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Cas9+mCherry+ RMS cells were then transiently transfected with pLentiguide-PURO plasmid and selected with 1 µg/ml Puromycin (InVivoGen, #ant-pr-1) for two days ...
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bioRxiv - Cell Biology 2024Quote: ... was a gift from Kristen Brennard and stably expressed in RPE1 cells using puromycin selection (10 ug/mL, Invivogen Cat#ant-pr-1). CA-YAP and dCas9-expressing cells were generated using third-generation lentiviral transduction ...
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bioRxiv - Microbiology 2024Quote: ... Human adenocarcinoma alveolar basal epithelial A549 cells overexpressing ACE2 and TMPRSS2 (‘A549-ACE2-TMPRSS2’) (Cat#: a549-hace2tpsa) was purchased from Invivogen (San Diego, CA). Human airway epithelial Calu-3 (Cat# ...
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bioRxiv - Cell Biology 2024Quote: ... Isogenic cells stably expressing LEXY from a doxycycline-sensitive promoter were selected by treatment with 200 µg/ml hygromycin B (InvivoGen, ant-hg-5).
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bioRxiv - Cell Biology 2024Quote: ... Two days after transfection cell were transferred to a 6-well plate and placed under selection of 100 µg/ml Zeocin (InvivoGen, San Diego, USA) for Fat4-Citrine constructs ...
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bioRxiv - Biochemistry 2024Quote: ... cells were covered with selection media comprised of DMEM + 10 % HI FBS + 1% PS and 2 μg/mL puromycin (Invivogen 58-58-2). Selection media was replaced every 48 h until control WT cells were completely dead ...
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bioRxiv - Immunology 2024Quote: ... 20μl of the HEK cell supernatant was transferred to a flat-bottom 96-well plate containing 180μl of QuantiBlue (Invivogen, cat. code rep-qbs) detection reagent and incubated at 37°C for 2 hours ...