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Citations for Invivogen :
1051 - 1100 of 1167 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Ultrapure LPS for cell culture experiments (lipopolysaccharide from Escherichia coli 0111:B4) was purchased from Invivogen (San Diego, CA, USA).
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bioRxiv - Immunology 2023Quote: Bioactive functional type I IFN was quantified in supernatants using the reporter cell line HEK-Blue IFN-α/β (Invivogen) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: Hela-S3 cells were purchased from ATCC and cultured in DMEM supplemented with 10 % heat inactivated FBS without antibiotics and incubated at 37 °C and 5 % CO2 Cells were tested regularly for mycoplasma with the PlasmoTest kit (InvivoGen) and are mycoplasma negative ...
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bioRxiv - Immunology 2023Quote: ... cells were pretreated with the caspase-1 inhibitor YVAD (Enzo) (1 μM) or with the NLRP3 inhibitor MCC-950 (Invivogen) (10 μM ...
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bioRxiv - Immunology 2024Quote: ... All the samples were collected for TLR4-stimulating activity using a cell-based colorimetric endotoxin detection kit (HEK-Blue LPS Detection Kit2, InvivoGen). Diluted samples were added to human embryonic kidney (HEK-293 ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: ... shControl and shLRBA HeLa cells were seeded at 0.3*106 cells in 6 well-plates followed by treatment for 16 h or 24 h with 5 μM of MG132 (InvivoGen) alone or in combination with 100 nM of Bafilomycin A1 for 3 h.
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bioRxiv - Molecular Biology 2024Quote: ... A549-ACE2 cells were further transduced with murine leukemia virus-based transduction vectors encoding TMPRSS2 and subsequently selected with blasticidin (Invivogen) to obtain A549-ACE2+TMPRSS2 cells ...
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bioRxiv - Immunology 2024Quote: ... PlatinumE cells were selected for 5 days using 1 µg/ml puromycin and 10 µg/ml blasticidin S (both Invivogen) and subsequently cultured ...
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bioRxiv - Genomics 2024Quote: ... was stably integrated at multiple copies into this cell line by Sleeping Beauty transposition then selected using 800 μg/mL G418 (InvivoGen). Monoclonal cell lines were isolated by sorting for cells expressing BFP and GFP and single-cell deposition.
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bioRxiv - Genomics 2024Quote: ... were integrated into cells by Sleeping Beauty transposition then selected using 800 μg/mL G418 and 10 μg/mL blasticidin (InvivoGen). Monoclonal cell lines were isolated by sorting for cells constitutively expressing GFP and expressing BFP upon treatment with 1 μg/mL doxycycline (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... Cells were recovered in media for 72 hrs and then cells that integrated the plasmid library by Bxb1 recombination were selected with 1 μg/mL puromycin (InvivoGen) for 7 days ...
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bioRxiv - Immunology 2024Quote: ... FL-BMDC cultures (2×105 cells per well) were stimulated with either 100 ng/ml profilin or 1 μM CpG-B/ODN1668 (Invivogen), or left unstimulated as a control in 200 ul DC medium in 96-well plates ...
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bioRxiv - Microbiology 2024Quote: ... or stimulated as follows to determine functional knockdown: AIM2 (cells were treated with ultra- pure LPS (100 ng/m, Invivogen) for 2 hrs ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 days after transfection the cells were transferred to 10 cm dish and 4-5 days after transfection cells were selected with 100 μg/ml Hygromycin (InvivoGen). All CRIS-PITCh plasmids are summarized in Table S1.
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bioRxiv - Molecular Biology 2024Quote: ... 293T and Calu-3 cells were transduced with murine leukemia virus-based transduction vectors encoding ACE2 and subsequently selected with puromycin (Invivogen), resulting in cell lines that stably expressed ACE2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Vero-TMPRSS2 cells were further transduced with murine leukemia virus-based transduction vectors encoding ACE2 and subsequently selected with puromycin (Invivogen) to obtain Vero-ACE2+TMPRSS2 cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Stably transfected 293 Flp-In T-REx derivative cell lines were cultured in a medium supplemented with 5 mg/mL Blasticidin (Invivogen) and 100 mg/mL Hygromycin B (Invivogen).
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bioRxiv - Microbiology 2024Quote: ... VK2/E6E7 cells were pre-incubated for 30 min at 37 °C with 1 μg/ml of anti-hTLR1 (mabg-htlr1, InvivoGen), anti-hTLR2 (MAB2616 ...
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bioRxiv - Immunology 2024Quote: ... Cell lines were passaged 2-3 times a week and tested for mycoplasma contamination on routine basis using Mycostrip (Invivogen). Cells were treated with indicated concentration of oligonucleotides or Enpatoran (MedChemExpress ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50 μL of supernatants from transfected cells were incubated with 30 μL of luciferase substrate (rep-qlc2, QUANTI-LucTM, InvivoGen), and the luciferase activity was measured using a spectrophotometer (Varioskan lux ...
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bioRxiv - Cell Biology 2024Quote: ... Targeted cells were transduced with the lentivirus and selected in medium containing puromycin (1 μg/mL, InvivoGen, #ant-pr-1) for 2-3 days ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µL of cell supernatant was transferred to a 96-well plate containing 180 µL of QUANTI-Blue assay solution (InvivoGen) to measure SEAP levels ...
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bioRxiv - Cell Biology 2024Quote: ... Jurkat cells stably expressing GFP were cultured in RPMI 1640 containing 10% (v/v) FCS and 5 μg/ml blasticidin (InvivoGen). Mouse thymocytes were cultured in RPMI1640 medium containing 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... of the resulting vector into MDAMB231 or BY549 cells and further selection on 5 µg/ml puromycin (InvivoGen, Toulouse, France), for 10–15 days ...
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bioRxiv - Cancer Biology 2024Quote: All cell lines were routinely verified by STR fingerprinting and confirmed mycoplasma negative using the PlasmoTest Kit (Invivogen: REP-PT1). SU-DHL-10 ...
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bioRxiv - Cell Biology 2024Quote: ... Stable HEK293 SNAP-EGFP and CLIP-EGFP cells were selected and cultured in growth medium supplemented with 100 µg/ml hygromycin B (Invivogen).
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bioRxiv - Immunology 2021Quote: ... HEK-Blue TLR3 and HEK-Blue TLR4 reporter cells (Cat. no.# hkb-htlr2, hkb-htlr3 and hkb-htlr4) were purchased from InvivoGen (USA). Human monocytic cells ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μL of the supernatant or an IFNα/β standard containing purified IFNα and IFNβ were added to 180 μL HEK-Blue cells (Invivogen) (50,000 cells ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/uL primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/m primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in E14 standard medium supplemented by 100 μg/m primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... The clonal Dia-C6 cells were used for lenti-sgRNA transduction followed by 20 µg/mL blasticidin (InvivoGen, ant-bl-1) selection and fluorescence cell sorting for mNeonGreen or mScarlet (see Plasmids ...
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bioRxiv - Physiology 2021Quote: ... transfection: Cells with 60-80% confluency were transfected with synthetic double-stranded RNA poly(I:C) (high molecular weight, InvivoGen #tlrl-pic) using lipofectamine 2000 following the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... A459/KZFP cells were cultured in F-12K medium with 1.0 μg/ml puromycin (Invivogen, San Diego, CA; #ant-pr-1). An A549 cell line stably expressing Cas9 (A549/Cas9 cells ...
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bioRxiv - Cell Biology 2022Quote: ... 24 and a Blasticidin resistance gene used for the initial selection of infected cells (10 µg/ml Blasticidin, Invivogen, ant- bl). MEF cells were cultivated in DMEM (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: B-ALL cells were infected with Cas9-2A-blast lentivirus followed by one-week selection with 5-15 µg/mL blasticidin (InvivoGen, CA). Cells were amplified and tested for Cas9 activity using the reporter assay described in Tzelepis et al.14 Pools with >75% Cas9 activity were used going forward ...
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bioRxiv - Microbiology 2022Quote: ... and TLR9 and TLR7 activation was determined by measuring secreted embryonic alkaline phosphatase (SEAP) reporter activity in cell culture supernatants by QuantiBlue™ reagent (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Microbiology 2022Quote: ... RIG-I stimulation was assessed by analyzing Lucia luciferase reporter gene expression in 20 µl cell culture supernatants using QUANTI-Luc™ (Invivogen). Data are expressed as the normalized fold change over mock infected cells ...
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bioRxiv - Microbiology 2021Quote: ... 50 µL of each cell supernatant were transferred into separate plates and added 50 µL of quantiblue reagent™ following manufacturer’s instructions (InvivoGen Inc.). Afterwards ...
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bioRxiv - Systems Biology 2021Quote: ... Different innate immune stimuli were applied to cell medium at 10% culture volume as follows: High molecular weight (HMW) poly(I:C) (Invivogen #tlrl-pic) at a concentration of 1mg/mL for 2 h (final concentration in the well 100 ug/mL ...
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bioRxiv - Systems Biology 2021Quote: ... 20 μL of the cell media supernatant was added to 50 μL of QUANTI-Luc™ assay solution (Invivogen #rep-qlc1) in a white 96-well microplate (Greiner ...
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bioRxiv - Cell Biology 2021Quote: ... Double knockout cells were selected by using a combination of 1μg/ml Puromycin and 6μg/ml Blasticidin (Invivogen, #ant-bl-10p) for 1 week.
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bioRxiv - Microbiology 2021Quote: ... HEK293-FT_hACE2 and A549_hACE2 cells were grown in their correspondent medium with 250-500 μg/ml of blasticidin (Invivogen # ant-bl-1). All cell media were supplemented with 8% (v/v ...
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bioRxiv - Immunology 2023Quote: The RAW-Blue ISG macrophage cell line stably transfected with an ISGF3-dependent reporter construct was cultured and maintained according to the manufacturer’s instructions (RAW-Blue ISG cells, Invivogen raw-isg). ISGF3 reporter activity was determined from cell culture supernatants using QUANTI-Blue detection reagents according to the manufacturer’s instructions (Invivogen).
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bioRxiv - Microbiology 2023Quote: ... Recombinant IgA1 produced in ExpiCHO-S cells as described above was purified using protein M agarose beads (Cat# gel-pdm-2, InvivoGen US) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: The inhibitory effect of APB-R3 on IL-18 signal transduction was tested using the HEK-BlueTM IL-18 reporter cell assay system (InvivoGen, US). APB-R3 and recombinant IL-18BP were appropriately diluted ...
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bioRxiv - Microbiology 2023Quote: The ADCC reporter assay was performed by using Jurkat-Lucia NFAT-CD16 cells (human FcγRIII, V158) (Invivogen, Cat# jktl-nfat-cd16) as effector cells according to the manufacturer’s instruction with modifications ...
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bioRxiv - Cancer Biology 2022Quote: ... Matrigel/cell mix was incubated for 15 min at 37C to allow for solidification and base media with Primocin® (InvivoGen) was added to top and bottom chambers of transwell ...
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bioRxiv - Bioengineering 2024Quote: All materials were verified to be free of endotoxins using the HEK-Blue mTLR4 reporter cell line (InvivoGen, San Diego, CA) using the protocol provided by the supplier ...
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bioRxiv - Immunology 2024Quote: ... differentiated for seven days with 50ng/mL M-CSF) and adherent peritoneal exudate cells (PECs) incubated with and without 200ng/mL lipopolysaccharide (LPS, InvivoGen, #tlrlpb5lps) for 6 hours (Fig S1D) ...