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Citations for Invivogen :
51 - 100 of 376 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The day following transfection cells were treated with selection antibiotics: 10 μg/ml blasticidin (Invivogen) and 50 μg/ml hygromycin B (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... transfection reagent (vehicle) and 2 μg/ml or 10 μg/ml ultrapure LPS-B5 (InvivoGen) prepared from E ...
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bioRxiv - Molecular Biology 2020Quote: ... cGAS functional cells were selected by fluorescence activated cell sorting after Y-DNA transfection (Invivogen). For cell treatments the following compounds were used ...
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bioRxiv - Immunology 2020Quote: ... and two healthy control (HC) donors were treated with STING inhibitor H-151 (5μM, InvivoGen) at 37°C for 4 hours ...
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bioRxiv - Microbiology 2019Quote: ... Scrambled control ssRNA (Table S2) was designed using siRNA wizard software (Invivogen, San Diego CA).
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bioRxiv - Immunology 2022Quote: Peritoneal macrophages from control or EROS-/- mice were primed overnight with LPS (100ng/mL, Invivogen) and treated for 2h with ATP (2.5mM ...
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bioRxiv - Bioengineering 2023Quote: ... and the control cell line HEK-Lucia™ Null cells (Catalog No. hkl-null, InvivoGen), and Mouse skin melanoma cells (B16-F10 ...
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bioRxiv - Immunology 2020Quote: ... and Poly:IC were all complexed with LyoVec transfection reagent and purchased from Invivogen (San Diego, California). Human CXCL10 was quantified using a commercial ELISA with ancillary reagent kit (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... Medium was replaced 1 days after transfection and cells were selected with 2µg/ml Puromycin (Invivogen). Cells were replated at 0.15×10^6c/w in a 96 well plate 5 days post differentiation ...
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bioRxiv - Molecular Biology 2023Quote: ... seeded at low density the day after transfection and selected with Ganciclovir (5 μg/mL, Invivogen) for one week ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibiotic selection was applied two days after transfection (2 μg/mL puromycin (#ant-pr-1, Invivogen) for 3 days).
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bioRxiv - Immunology 2022Quote: ... mouse TLR2 neutralizing antibody monoclonal mouse IgG2a (C9A12) and control isotypes mouse IgG2a were from InvivoGen. Mouse TLR4/MD2 complex neutralizing antibody monoclonal rat IgGk clone MTS510 and control isotypes rat IgGk were from eBioscience™ Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... pneumoniae and control reagents were resuspended or diluted in pre-warmed HEK-BlueTM Detection medium (Invivogen). 5 x 104 HEK-BlueTM hTLR2 cells were mixed with 5 x 105 CFU S ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of high molecular weight poly-(I:C) was transfected per 1e6 cells using LyoVec transfection reagent (Invivogen). To stimulate type I IFN signaling ...
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bioRxiv - Cell Biology 2022Quote: ... one day post-transfection cells were treated with 1 μg poly(I:C) HMW (InvivoGen, San Diego, CA) and 1 μg/mL dox per well ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of high molecular weight poly-(I:C) was transfected per 1e6 cells using LyoVec transfection reagent (Invivogen). To stimulate type I IFN signaling ...
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bioRxiv - Neuroscience 2023Quote: ... Twenty-four to 72 h after transfection they were selected with 0.5 µg/mL of Puromycin (Invivogen). Individual cells were allowed to recover into single colonies ...
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bioRxiv - Molecular Biology 2022Quote: ... the FTO-targeting and Control shRNAs were designed in silico with Blastn (NCBI) and siRNA wizard (Invivogen) online tools ...
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bioRxiv - Immunology 2020Quote: The following PRR agonists were used as controls in various experiments: Pam3CysSerLys4 (Pam3CSK4, TLR1/TLR2 agonist, InvivoGen); Lipopolysaccharide (LPS ...
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bioRxiv - Immunology 2022Quote: ... Each plate included a healthy donor positive and negative control with the Phorbol myristate acetate (PMA, InvivoGen) to confirm reagent performance ...
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bioRxiv - Microbiology 2022Quote: ... Positive controls were conducted by the addition of 1.25 µg/mL triDAP 6 hpi (InvivoGen, Toulouse, France). Stimulation of NOD1 was measured at OD600 nm 18 hpi and 24 hpi (corresponding to treatment durations of 12 h and 18 h ...
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bioRxiv - Cell Biology 2022Quote: ... Transfected cells were selected 24 h after transfection with either 10 μg·mL-1 Blasticidin S or 10 μg·mL-1 G418 (both InvivoGen). Gene disruptions were confirmed either by immunoblotting (forB- and forG- ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfection media was aspirated and reduced serum media with 10 μg/mL of Poly(I:C) (InvivoGen, California, USA) was added to the cells for three days ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cell transfection and infection of target cells was performed by Calcium Chloride followed by hygromycin B (InvivoGen) 0.5 mg/mL selection for 8 days.
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BET protein inhibition regulates macrophage chromatin accessibility and microbiota-dependent colitisbioRxiv - Immunology 2021Quote: ... or vehicle control for 12 hours followed by the addition of 50 ng/mL LPS (InvivoGen, #tlrl-peklps) for 4 hours ...
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bioRxiv - Immunology 2021Quote: ... TLR4 (HEK-TLR4) and HEK-TLR2-blue and control HEK cell line (HEK-null) were purchased from InvivoGen and cultured in DMEM supplemented with 10 % FCS ...
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bioRxiv - Immunology 2021Quote: ... was added to the culture at 0.5μg/mL or vehicle control (endotoxin-free water) was added at a matched volume (InvivoGen). On day 2 of culture ...
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bioRxiv - Immunology 2023Quote: ... Cat# tlrl-hprna), LyoVec (Cat# lyec-1) and dispersible whole glucan particles (WGP, Cat# tlrl-wgp) were purchased from InvivoGen. Recombinant murine IFN-β1 (Cat# 581304 ...
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bioRxiv - Cancer Biology 2022Quote: ... Primocin (InvivoGen, Cat# N001), 1% Noggin conditioned medium (U-Protein Express ...
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bioRxiv - Biophysics 2023Quote: ... + Primocin (Cat. No.NC9392943, Invivogen) in an incubator at 37°C with 5% CO2. ...
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bioRxiv - Microbiology 2019Quote: ... in 12-well plates were transfected with 0.2 µg of plasmid DNA per well using LyoVec transfection reagent (InvivoGen), according to manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were replated in 96-well plates the next day at 2 × 104 cells/well density and transfected with poly I:C (700 ng in 110 μl volume) using Lyovec transfection reagent (InvivoGen). After 20 hours ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The selection was started 48 hours after transfection using growth media supplemented with 500µg/ml Hygromycin Gold (ant-hg-1, Invivogen). Selection media was changed every 3-4 days until confluent colonies of stable transgenic cells had formed and cells were ready to be split.
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bioRxiv - Molecular Biology 2021Quote: ... The M1 cells with stable CD9-EGFP expression was generated by transfection with pcDNA3.1 CD9-EGFP using Metafectene Pro (Biontex) and selection with G418 (InvivoGen). HEK293T cells were maintained in Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Microbiology 2023Quote: ... in 24-well plates were transfected with 0.1 µg of plasmid DNA per well using LyoVec transfection reagent (InvivoGen), according to manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: Activity of the secreted Lucia luciferase under IRF3 promoter control (THP1 Dual cells) was assessed using QuantiLuc substrate (Invivogen) according to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... and cells were stimulated with 100 μl milk EVs or EV-depleted controls in the presence or absence of 5 μg/ml Poly I:C (Invivogen). After 4 or 5 hours ...
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bioRxiv - Immunology 2022Quote: ... Positive control stimulation of microglia was also performed by the addition of LPS (100 ng/mL; Invivogen tlrl-b5lps) and ...
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bioRxiv - Immunology 2019Quote: ... 293/NOD2 cells were obtained by stable transfection of HEK293 cells with the pUNO-hNOD2 plasmid which expresses the human NOD2 gene (Invivogen). THP1 cells were obtained from ATCC.
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bioRxiv - Microbiology 2019Quote: ... Post transfection freshly egressed parasites were resuspended in DMEM supplemented with 50 μg/mL of Zeocin (InvivoGEN, 11006-33-0) for 4 hour and then added to HFF monolayers supplemented with 5μg/mL Zeocin to select for integrants ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human antibodies were expressed in HEK-293T cells following transient transfection with pairs of the IgG1 heavy and light chain expression plasmids according to protocols from Invivogen. Recombinant antibodies were purified using Protein A sepharose (IBA ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5nM WR99210 (Jacobus Pharmaceuticals, Princeton, NJ) was added 6-8hours post transfection for the pSLI construct and with blasticidinS (Invivogen) at 2 μg/ml for the pDC2 plasmid ...
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bioRxiv - Microbiology 2021Quote: Human embryonic kidney HEK293T/17-hACE2 cells with stable expression of human angiotensin-converting enzyme ACE2 (AXON Neuroscience SE) were prepared by stable transfection of pDUO2-hACE2-TMPRSS2a (InvivoGen) with transfection reagent Lipofectamine 3000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... or 7.7G6 mAb were produced in HEK-293T cells following transfection with pairs of the IgG1 heavy and light chain expression plasmids according to protocols from InvivoGen. Human antibodies were purified from cell culture supernatants using Protein-A affinity chromatography ...
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bioRxiv - Cancer Biology 2020Quote: ... transient transfection of HEK-293FT cells with packaging and lentiviral vector plasmids were performed using LENTI-Smart INT kit (InvivoGen) following manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... and 1000 ng/mL) was transfected into the cells using LyoVec cationic lipid-based transfection agent (Catalog Code, lyec-12, InvivoGen). Briefly ...
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bioRxiv - Systems Biology 2023Quote: ... Transfected cells were incubated for 24 hours before transfection media was replaced with media containing 10 ug/ml blasticidin (Invivogen). Cells were incubated for 30 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transferred to a 10 cm dish the day after transfection and selection with Puromycin (2 μg/mL, InvivoGen) was started within 48 h of transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Three days post-transfection cells were sub-cultured into media supplemented with puromycin (0.8 µg/mL; Invivogen, ANT-PR-1). After six days of selection ...
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bioRxiv - Immunology 2021Quote: ... normocin (cat#ant-nr-1) and zeocin (cat#ant-zn-05) were from InvivoGen. K777 [K11777] (cat#AG-CR1-0158-M001 ...