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Citations for Biotium Inc. :
151 - 166 of 166 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 100 µL 2x copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction buffer was added to cells to append a fluorophore reporter (2x concentrations: 10 µM CF488A picolyl azide (Biotium #92187), 2 mM CuSO4 (Sigma C8027) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Cell Biology 2021Quote: At 48h post-infection cells were incubated with 300μl of 4 μM Calcein-AM and 2 μM EthD-III from the Viability/Cytotoxicity Assay Kit for Animal Live and Dead cells (Biotium 30002-T) for 45 min at room temperature as per manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: Viability of monolayers post-antibiotic treatment was assessed using the Viability/Cytotoxicity Assay for Animal Live & Dead Cells kit (Biotium, Fremont, CA) according to package insert ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from the cell pellets using the hot-phenol procedure and followed by biotinylation with MTSEA-Biotin-XX (Biotium, USA, Fremont) as previously described (27 ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed with DPBS and processed for either immunoblotting staining or were directly stained with WGA CF®488A (Biotium®) for 30 minutes at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... 1×106 cells from each liver were stained for various cell markers: Live-or-Dye™ fixable viability dye in APC-Cy™7 (Biotium, Cat ...
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bioRxiv - Microbiology 2024Quote: ... The bladder cells were stained for membrane (CellBrite Steady Membrane 405) and nucleus (NucSpot Live Cell 650) for 30 minutes in pre-warmed EpiLife as per manufacturer’s instructions (Biotium, San Francisco, USA). After the staining step and two washes with 1 x PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The reporter gene construct was transiently transfected to each stable cell line and luminescence measured using Firefly Luciferase Assay Kit (Biotium, Inc., Fremont, California) and a Wallac 1420 VICTOR2 microplate reader (PerkinElmer ...
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bioRxiv - Bioengineering 2022Quote: ... Aliquots of detached cells (approximately 2×106) were incubated with a series of CF®640R succinimidyl ester-labeled (Biotium, USA, cat. 92108) ACE2 extracellular domain (AA Q18 – S740 ...
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bioRxiv - Bioengineering 2022Quote: ... the cell aggregates were processed and imaged as described in the whole-mount immunofluorescence methods using Streptavidin (29072, Biotium, 1:500, CA, USA) and a DAPI (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... by IP3R-mediated ER Ca2+ release were measured in cells loaded with membrane-permeant AM ester form of Fluo-3 (Fluo-3-AM) (Biotium, Fremont, CA, USA) and Fura-Red ...
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bioRxiv - Microbiology 2023Quote: ... and DNA from membrane-compromised bacterial cells was removed by treatment of coral homogenates and tissue airbrushed blanks with the viability dye PMAxxTM (Biotium, Fremont, CA, USA). PMAxx is a DNA-intercalating agent that forms photo-induced crosslinks making the bound DNA inaccessible for downstream molecular applications ...
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bioRxiv - Cell Biology 2022Quote: ... Cell viability was evaluated in the control condition (cells grown in the complete medium) and in the test conditions (cells grown in the hyperosmolar media) via a resazurin-based assay (Biotium, Inc., Fremont, CA, USA) performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were dried and slides were coverslipped using fluorescent mounting medium (VectaShield, Vector Laboratories, Inc., Burlingame, CA, USA; or EverBrite, Biotium, Inc, Fremont, CA USA). In some studies ...