Labshake search
Citations for Biotium Inc. :
101 - 150 of 166 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Cells were stained with Lysotracker red (Thermo) or Alexa594-labeled cholera toxin subunit B (CTB, Biotium) according to manufacturer’s protocols ...
-
Proteasomal degradation of human SERINC4: a potent host anti-HIV-1 factor that is antagonized by NefbioRxiv - Microbiology 2020Quote: ... cells were lysed and intracellular luciferase activities were determined using Firefly Luciferase Assay Kit 2.0 (Biotium). These luciferase activities were used to calculate viral infectivity.
-
bioRxiv - Microbiology 2023Quote: ... the cells were treated with 1 µg ml-1 nuclear stain NucSpot 650/665 (#41034, Biotium) for 30 min at RT ...
-
bioRxiv - Systems Biology 2023Quote: ... Cells were then mounted onto glass slides with EverBrite mounting medium with DAPI (Biotium Cat #23002), and ready for imaging ...
-
bioRxiv - Bioengineering 2023Quote: Organoids were assayed for cell viability using a live/dead reagent kit (Biotium, Catalog #30002-T). Please see Sup ...
-
bioRxiv - Cell Biology 2023Quote: ... Fixed cells were additionally stained with membrane marker CellBrite (dilution 1:200, Biotium, Fremont, CA, USA) and actin marker Phalloidin (dilution 1:1000 ...
-
bioRxiv - Biophysics 2024Quote: ... the cells were incubated in AnV buffer (1:175 dilution of the fluorescently tagged AnV (Biotium) in Hank’s balanced salt solution ...
-
bioRxiv - Genomics 2023Quote: ... Cells were then incubated in 0.1 mg/ml Wheat Germ Agglutinin (WGA) CF770 conjugate (Biotium 29059) and 0.5 μg/ml DAPI (Abcam ab285390 ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were washed twice and loaded with 25 nM TMRM and either 5 μM Nucview 488 (Biotium) or 5 μM Fluo4-AM (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then washed several times for 2hrs in BB and mounted on glass with EverBrite (Biotium). For primary and secondary antibodies see Immunofluorescence microscopy and antibodies section.
-
bioRxiv - Bioengineering 2022Quote: ... the plasma membrane of live cells were stained with Wheat Germ Agglutinin (WGA) (CF633, Biotium, Fremont, CA). Cells were washed twice in Hank’s Balanced Salt Solution (HBSS ...
-
bioRxiv - Microbiology 2021Quote: ... cells were stained with Wheat Germ Agglutinin (WGA) Alexa Fluor 488 Conjugate (Biotium; Catalog no. 29022-1) at a final concentration of 0.1 mg/mL ...
-
bioRxiv - Bioengineering 2021Quote: ... the plasma membrane of live cells were stained with Wheat Germ Agglutinin (WGA) (CF633, Biotium, Fremont, CA). Cells were washed twice in Hank’s balanced salt solution (HBSS ...
-
bioRxiv - Bioengineering 2021Quote: ... the cells were fixed with 4% PFA and stained with CF532 Wheat Germ Agglutinin (WGA) Conjugates (Biotium) and DAPI and imaged under a confocal microscope (Leica SP8) ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were pre-treated 16-20 h with CellBrite®650 according to the manufacturers’ instructions (Biotium). For the analysis of ALIX-vesicle motility ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were pre-incubated with Fura-2·AM or Fluo-4·AM (30 minutes, 1 μM, Biotium) at 37°C with 5% CO2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Then, cells were resuspended in PI Staining Solution (3.8mM sodium citrate, 50 μg/ml PI (Biotium, USA), 40 μg/ml RNase A (Zymo Research ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were exposed to 10 μM SynaptoRed™ C2 (Equivalent to FM®4-64; Biotium; 70027) in Stimulation Buffer consisting of 31.5 mM NaCl ...
-
bioRxiv - Physiology 2023Quote: ... Imaging of microtubules was performed by staining the microtubules with ViaFluor Live Cell Microtubule Stain 647 (Biotium) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were blocked for 1 hour at room temperature with 1x Fish Gelatin Blocking Agent (Biotium, #22010) in wash buffer ...
-
bioRxiv - Molecular Biology 2023Quote: TUNEL assay was used to detect cell apoptosis using the CF605R TUNEL Assay Apoptosis Detection Kit (Biotium) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then washed twice with 1X PBS and then incubated with TrueBlack IF Background Suppressor (Biotium) for 30 minutes at RT ...
-
bioRxiv - Cancer Biology 2024Quote: ... The cells were then incubated with 25 µM Alexa Fluor 568-hydroxylamine (Fluoroprobes) or CF680R-Aminooxy (Biotium) in the presence of 10 mM aniline (Sigma ...
-
bioRxiv - Immunology 2021Quote: ... cells were stained with 1 µg/mL of wheat germ agglutinin (WGA) conjugated to CF568 (Biotium; #29077-1) and 1 µg/mL anti-CD154 (CD40L ...
-
bioRxiv - Plant Biology 2021Quote: ... protonemal cells were stained by the 5 µM of fluorescent dye SynaptoGreen C4 (equivalent to FM1-43, Biotium, USA ...
-
bioRxiv - Molecular Biology 2019Quote: ... cells were washed twice with PBS and incubated with PBS containing 50 μg/mL propidium iodide (Biotium, 40017) and 100 μg/mL RNase A (Omega ...
-
bioRxiv - Neuroscience 2022Quote: Lysosomal activity was measured for the BV2 cell line and iMGLs using the LysoViewTM 540 reagent (Biotium, 70061) and Incucyte S3 Live-Cell imaging ...
-
bioRxiv - Biophysics 2023Quote: ... After removal of excess NeutrAvidin the flow cell is incubated with 150 pM biotinylated NTA beads (#90074, Biotium) for 3 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1 h at 37°C in mKRB supplemented with native coelenterazine (5 µM, BIOTIUM), and then incubated for 15 minutes in mKRB supplemented with either TPEN (500 µM ...
-
Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... cells were rinsed and 1:1000 CellBrite Steady 650 membrane dye and 1:1000 kit enhancer (Biotium #30108) was added in Fluorobrite DMEM +10% FBS for 30 minutes at 37℃.
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were then washed with annexin-binding buffer and additionally incubated with NucSpot Live 650 (1:1,000; Biotium) for 1 hr at 37 °C ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed with PBS three times and coverslips were mounted onto glass slides in EverBrite Mounting Medium (Biotium). Cells were imaged using an EVOS FL microscope (Life Technologies) ...
-
bioRxiv - Cancer Biology 2023Quote: ... The Live-or-DyeTM Zombie Aqua fixable viability staining kit was used for live cell analysis (32004-T; Biotium). For quantification ...
-
bioRxiv - Biophysics 2023Quote: ... the growth medium was removed and cells were incubated with 1 μM CellBrite™ NIR680 (Biotium, Fremont, CA, USA) for 20 min ...
-
bioRxiv - Immunology 2023Quote: ... Dead cells and doublets were excluded from analyses based on staining of 7-amino-actinomycin D (7-AAD) (Biotium) or Zombie yellow (catalog ...
-
bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
-
bioRxiv - Cell Biology 2023Quote: ... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
-
bioRxiv - Neuroscience 2024Quote: ... cocultures exposed to TSZ after 4.5 h after were stained using a propidium iodide-based (PI) cell viability kit (Biotium) according to manufacturer protocols ...
-
bioRxiv - Neuroscience 2022Quote: ... and 48 h later luciferase activity was measured in cell lysates using the Firefly & Renilla Single Tube Luciferase Assay Kit (Biotium), according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... flow cytometery was used to assess mitochondrial number and membrane potential of the cells treated with Mito Tracker (Biotium, 70075) and TMRE (tetramethylrhodamine ethyl ester ...
-
bioRxiv - Immunology 2020Quote: ... sialic acid residues at the cell surface were oxidized using sodium meta-periodate (Thermo) and biotinylated with aminooxy-biotin (Biotium). Following quenching ...
-
Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... then maintained in complete darkness for two days prior to incubation with a cell viability stain (Live-or-Dye 488/515 (Biotium). Briefly ...
-
bioRxiv - Genetics 2020Quote: To test the relative expression of XIST across cell lines quantitative real-time PCR (qPCR) was carried out using EVAgreen (Biotium) and Maxima Hot Start Taq (Thermo Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... cells were centrifuged to pellet (table-top centrifuge, 3000 xg, 15 min) and resuspended in PBS containing MitoView 633 (Biotium). MitoView 633 accumulates in the inner membrane as a function of membrane potential ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested on day 5 and the proportion of apoptotic cells was assessed using the NucView 488 Caspase-3 Assay Kit (Biotium) according to the manufacturer’s instructions and an Attune NxT flow cytometer (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... The washed cells were stained with 0.1 mg/ml CF®488A wheat germ agglutinin conjugate (Biotium, Fremont, CA, USA) in PBS for 5 min at room temperature and washed two more times with PBS buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were then re-hydrated in PBS for 10 minutes before blocking with TrueBlack® IF blocking buffer (Biotium #23012B) for 10 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... Infected cells were then washed with PBS buffer (pH 7.4) and resuspended in complete RPMI containing NucSpot® Live 488 (Biotium, #40081) following the manufacturer recommendations ...
-
bioRxiv - Microbiology 2022Quote: ... immediately spread as a single-cell layer between a quartz slide and coverslip and sealed with a CoverGrip sealant (Biotium, USA). Two-dimensional Raman maps were obtained with a laser excitation at 532 nm (20 mW power at the focal plane ...
-
bioRxiv - Cell Biology 2019Quote: ... The Calbryte-590 loaded cells were then incubated in HBSS buffer containing 0.5 µg/mL CF 488-tagged-Annexin-V (Biotium, cat. 29011), a fluorescence PS probe ...