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Citations for Biotium Inc. :
151 - 200 of 506 citations for 7 HYDROXY 5 METHYL 1 3 4 TRIAZAINDOLIZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... BRET signals were recorded from microsomes (each ∼10 µg) in the presence of 5 µM coelenterazine (Biotium Corp.) using the Cytation 5 image reader (BioTek Instruments) ...
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bioRxiv - Cell Biology 2022Quote: ... BRET signals were recorded from microsomes (each ∼10 μg) in the presence of 5 µM coelenterazine (Biotium Corp.) using the Cytation 5 image reader (BioTek Instruments ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was denatured at 60°C for 10 min and biotinylated using 5 µg/mL biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
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bioRxiv - Immunology 2021Quote: ... the inserts were transferred to a fresh 24-well plate with black walls containing 500 μl of 4 μM Calcein AM (Biotium) in mHBSS per well ...
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bioRxiv - Immunology 2019Quote: ... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... we utilized fluorophore-conjugated forms of the lectin wheat germ agglutinin (WGA) (Biotium, 29022- 1, 29062-1, 29025-1) to outline all cellular membranes ...
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bioRxiv - Biochemistry 2021Quote: Polymerase activity from 5°C to 50°C was measured with EvaEZ™ Fluorometric Polymerase Activity Assay Kit (Biotium) in the provided Tris buffer system based on the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were incubated in a Flexstation 3 for at least 60 minutes at 37°C in Ca5 dye plus 1.25 mM probenecid (Biotium #50027, Jomar Life Research, Australia) prior to the start of recording ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated Donkey anti-Sheep IgG (for ChAT, 1:250, #20191-1, Biotium). All antibodies were diluted in the blocking solution.
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bioRxiv - Neuroscience 2023Quote: ... along with 1% BSA and mixed 1:200 with RedDot (40060, Biotium). The samples were then isolated from debris by fluorescence -activated nuclei sorting (FANS) ...
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bioRxiv - Genomics 2021Quote: ... 1 × EvaGreen dye (Biotium) was added to the amplification mixture to monitor the PCR amplification ...
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bioRxiv - Cell Biology 2024Quote: ... + 1:1000 Enhancer (Biotium) and incubated for ∼10-15min before starting imaging ...
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bioRxiv - Cell Biology 2023Quote: ... antibodies were used at 1μg/mL overnight at 4°C and were subsequently detected with STORM-appropriate secondary antibodies (Biotium; 20836 and 20811). STORM acquisition was facilitated with an N-STORM system (Nikon Instruments ...
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bioRxiv - Genomics 2019Quote: ... buffer at 5 V/cm with a pulse time of 30 s for 24 hours and stained in 3X Gel Red (Biotium) before imaging ...
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bioRxiv - Neuroscience 2021Quote: ... All oocytes used for TEVC and VCF experiments had been previously labelled in the dark with 5 μM CF488 maleimide (Biotium) or AlexaFluor488 maleimide (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 μL of aniline (diluted 1:10 in water) and 1 μL of 100 mM biocytin hydrazide (Biotium, 90060). The reaction proceeded while rotating at 4°C for 90 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... multi-shank neural probe (NeuroNexus A4×4-3mm-100-125-177-A16, Ann Arbor, MI, USA) pre-coated with DiI (Biotium, Fremont, CA, USA) for fluorescent detection of recording sites was advanced dorsoventrally at 5 µm/s through the IC using a hydraulic micropositioner (Model 650 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed and incubated for 5 minutes at 37°C with 1X Pre-Staining Solution (MemBrite® Fix 543/560, #30094, Biotium) diluted in DPBS buffer ...
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bioRxiv - Neuroscience 2023Quote: ... all proteins were used at a final concentration of 5 µM for this assay and GloMelt™ Thermal Shift Protein Stability Kit (Biotium) was used at final concentration of 0.5X ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with RedDot™1 Far-Red Nuclear Stain (1:200, Biotium) at room temperature for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... goat Anti-Guinea Pig IgG Polyclonal CF-488A (1:500, Biotium, cat#: 20017-1), cy5-AffiniPure Donkey Anti-Guinea Pig IgG H&L (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Phalloidin CF405 (1:200; Biotium) or MitoSpy™ NIR DiIC1 dye (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... Hoecsht 33342 (1:500; Biotium), and rhodamine-phalloidin (1:20 ...
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bioRxiv - Neuroscience 2022Quote: ... DAPI (1:2000; Biotium 40011), Hoechst (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 x EvaGreen dye (Biotium), 3 µl DNA template dilution (1×104 copies/reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 x EvaGreen dye (Biotium), 3 µl DNA template dilution (1×104 copies/reaction ...
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bioRxiv - Genetics 2022Quote: ... DAPI (Biotium#40043, 1:500) was used as a nuclear counterstain ...
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bioRxiv - Cell Biology 2021Quote: ... Phalloidin CF647 1:100 (Biotium) in PBS + 1% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... and DAPI (1:1000, Biotium). Epidermal cells for RNA-sequencing experiments were membrane GFP+ ...
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bioRxiv - Cell Biology 2022Quote: ... and DAPI (1:1000, Biotium). We used Flow cytometry was performed on a BD LSRII cytometer (BD Biosciences) ...
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bioRxiv - Bioengineering 2022Quote: ... and 1× EvaGreen (Biotium, 31000). 5 ng of the purified PCR1 of each sample was used as input for the final PCR2 ...
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bioRxiv - Genomics 2023Quote: ... and 1× EVA green (Biotium). The qPCR (Bio-Rad CFX Real-Time system ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed 3 times for 5 min with PBS to remove unbound antibody and subsequently stained with CF660C labeled anti-rabbit antibody (20183, Biotium, Fremont, CA) diluted 1:150 in PBS containing 5% (v/v ...
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bioRxiv - Genetics 2021Quote: The quality of DNA was evaluated by electrophoresis using 0.8 % (w/v) agarose gels stained with 5 μL/100 ml Gel Red® (Biotium Inc., USA) to enable visualization ...
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bioRxiv - Neuroscience 2023Quote: DRG neurons grown on coverslips as described above were first treated with a blocking solution consisting of sterile HBSS with 10 mM HEPES + 1% horse serum for 5 minutes at room temperature followed by staining with HBSS/HEPES + 70 mM Di-8-ANEPPS (Biotium, Fremont, CA) for 15 minutes room temperature and recovery in HBSS/HEPES + 10% horse serum for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... and WGA488 (1 μg/ml; Wheat Germ Agglutinin CF®488A Conjugate; Biotium, #BT29022-1) membrane staining was added to single wells immediately before imaging on an Opera Phenix™ High-Content Screening System (PerkinElmer).
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bioRxiv - Bioengineering 2023Quote: ... a PDMS well was filled with a 1:1000 dilution of YO-PRO-1 (Biotium), which was then incubated for an hour ...
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bioRxiv - Molecular Biology 2021Quote: ... The extension products were analysed on 1% agarose gel containing GelRed (1:10,000 v/v, Biotium) in TAE buffer ...
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bioRxiv - Microbiology 2023Quote: ... the cells were treated with 1 µg ml-1 nuclear stain NucSpot 650/665 (#41034, Biotium) for 30 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... biocytin (Biotium 90055, 1 mg/ml) was added to the intracellular fluid to verify recording location ...
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bioRxiv - Cell Biology 2022Quote: ... and rhodamine-phalloidin (1:20; Biotium) in permeabilization/blocking buffer at room temperature for 2 hours in the dark ...
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bioRxiv - Neuroscience 2021Quote: ... CF555 (1:2000, 20030, 20033 Biotium), Affinipure Cy5 (1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... and TrueBlack (1:5000, Biotium, 23007) in 1x PBS supplemented with Horse serum (4% ...
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bioRxiv - Microbiology 2022Quote: 10,000x GelRed (Biotium, catalog #: 41003-1).
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bioRxiv - Developmental Biology 2022Quote: ... 1 µL LipidSpot 610 (1000x) (Biotium) in DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... and TrueBlack (1:5000, Biotium, 23007). Images were acquired with a high-content screening confocal microscope (Opera Phenix ...