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Citations for Biotium Inc. :
1 - 50 of 506 citations for 7 HYDROXY 5 METHYL 1 3 4 TRIAZAINDOLIZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium, 30029). Cells were treated with 10 µL of drug-containing media ...
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bioRxiv - Immunology 2020Quote: Cell death within tumor slices was assessed using a green-fluorescent caspase 3/7 probe that binds DNA upon cleavage by caspase 3/7 (Nucview, Biotium). 2.106 untransduced or EGFR CAR T cells were applied onto BxPC3 tumor slices that were subsequently incubated with 5μM of NucView dye ...
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bioRxiv - Microbiology 2022Quote: ... NucView 488 fluorescent caspase-3/7 substrate (Biotium, Fremont, CA, USA) was added at a final concentration of 1.25µg/ml to visualize cells undergoing apoptosis ...
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bioRxiv - Cancer Biology 2021Quote: ... fluorescent caspase 3/7 substrate for detecting apoptosis by live staining (Biotium) at 2 µM was prepared with dosing medium (M199 +2% v/v FBS) ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
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bioRxiv - Cancer Biology 2023Quote: ... then were incubated for 10 minutes at 21 °C in 1 μM 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM, Biotium) in PSS ...
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bioRxiv - Cancer Biology 2020Quote: ... and 7 using CalceinAM (Biotium, CA; 148504-34-1), Ethidium Homodimer I (Biotium ...
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bioRxiv - Cell Biology 2023Quote: ... Wash Buffer with 1 mM ADVASEP-7 (Biotium; 70029), and finally Wash Buffer alone ...
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bioRxiv - Plant Biology 2021Quote: ... 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) from Biotium (CA, USA), Wortmannin (WM ...
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bioRxiv - Neuroscience 2022Quote: ... ADVASEP-7 (Biotium) was also resuspended at 500 μM in HBSS ...
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bioRxiv - Neuroscience 2024Quote: ... and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate, Biotium, 60014-10mg) were dissolved in 100% ethanol (Koptec ...
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bioRxiv - Neuroscience 2023Quote: ... Biocytin (1-3 mg/mL, Biotium) was dissolved in the pipette solution for post-hoc identification of recorded cells.
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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bioRxiv - Genomics 2022Quote: ... 7-AAD (Biotium, 40037) was stained to exclude dead cells ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
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bioRxiv - Biochemistry 2021Quote: ... The pHi of ArchT transfected RPE cells and control RPE cells were directly calculated using 2’,7’-bis(carboxyethyl)-5(6)-Carboxyfluorescein (BCECF, Biotium). BCECF is a ratiometric dual excitation/single-emission pH biosensor (405 ex/525 em for pH insensitive fluorescence ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with 5 μmol/L NucView™ 488 caspase-3 substrate (Biotium). Images were obtained in phase contrast and fluorescence mode using a x10 objective and an IncuCyte FLR time-lapse fluorescence microscope (Essen Bioscience) ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The larvae were housed in 10 μM INK128 (or DMSO) containing water and at 36 hpa they were treated with 5 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Biotium, #10058) for 55 min ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS (3 × 5 min) and treated with Trueblack (Biotium, Cat# 23007) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Neuro-DiI (7% in ethanol; Biotium) was slowly injected ...
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bioRxiv - Neuroscience 2020Quote: ... Neuro- DiI (7% in ethanol; Biotium) was slowly injected ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were replaced with medium containing 5 µM NucView 488 caspase 3 substrate (Biotium Inc., #10403). After 30 min incubation at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 u ml−1 phalloidin CF555 (Biotium, 00040) were added together with the secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Dead cells and doublets were excluded from analyses based on staining of 7-amino-actinomycin D (7-AAD) (Biotium) or Zombie yellow (catalog ...
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bioRxiv - Microbiology 2022Quote: ... samples were placed on a microscope slide with 0.8% agarose gel containing 5 μM Bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)) (Biotium) and 0.75 μM permeability reporter PI (MP Biomedicals) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested on day 5 and the proportion of apoptotic cells was assessed using the NucView 488 Caspase-3 Assay Kit (Biotium) according to the manufacturer’s instructions and an Attune NxT flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with 1 μM calcein-AM (Biotium, cat no. 80011-3) for 10 mins ...
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bioRxiv - Cell Biology 2021Quote: ... fluo-3 acetoxymethyl (Fluo-3 thereafter) ester (Biotium, US) following a protocol adapted from (Zhang et al. ...
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bioRxiv - Molecular Biology 2023Quote: The free radical probe 2’,7’-dichlorodihydrofluoresceindiacetate (H2DCFDA) (Biotium, Fremont, CA) was used to quantify ROS ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 hr at room temperature with 5% non-fat dry milk (Apex #20-241) or 5% BSA (Biotium #22013) in TBST (10 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... 16 xg at 4°C and the pellet resuspended and incubated in WGA488 stain (Biotium, 5 μg/mL, diluted in HBSS) for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: 5 mg of RNA was incubated with 2.5 mg biotin-conjugated MTSEA (1 mg/ml) (Biotium 90066-1) in MTSEA buffer [20 mM HEPES pH 7.3 (Quality Biological A611-J848-10) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated at 4°C for 4 h with CF488-conjugated anti-mouse IgG (1:2000) and CF568-conjugated anti-rabbit IgG (1:2000) antibodies (Biotium) diluted in PBS containing 2% FBS as secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 uL of 1 mM of SGC5 (Cat. number 70057, Biotium Inc., Fremont, CA) was injected into the AC of an enucleated mouse eye ...
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bioRxiv - Neuroscience 2019Quote: ... which we measured in the presence of 10−7 M tetrodotoxin (TTX; Biotium). TTX blocks sodium channels and therefore removes all action potential activity ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was run on a ViiA 7 with SYBR green mastermix (Biotium). 18S was used as the human cell housekeeping gene and Ywhaz was used as the mouse housekeeping gene ...
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bioRxiv - Cancer Biology 2021Quote: ... Activity of caspase-3 was detected with NucView 488 Caspase-3 Assay kit (Biotium) according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2023Quote: ... loaded with membrane-permeant AM ester forms of Fluo-3 (Fluo-3-AM) (Biotium) and/or Fura-Red ...
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bioRxiv - Cell Biology 2021Quote: ... or Proteostat (3 μM, Biotium) solutions.
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bioRxiv - Biochemistry 2024Quote: ... stained with 3 × GelRed (Biotium) for 30 min and visualized by using Chemidoc™ (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in DMEM 1% penicillin/streptomycin with 5 nM MitoView 533 (Biotium, #70055). Plates were incubated at 37°C for 30 min protected from light ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5 μL of 5 μg/mL Propidium Iodide (Biotium) for cell death detection ...
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bioRxiv - Neuroscience 2019Quote: ... then diluted to 6x) and run on agarose gels (1-2%) with 4% GelRed (Biotium, California, USA).
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bioRxiv - Neuroscience 2023Quote: ... cells were pre-incubated with Fura-2·AM or Fluo-4·AM (30 minutes, 1 μM, Biotium) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... loaded with Fluo-3-AM (Biotium) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... After at least 20 minutes of washout with aCSF containing 100 µM Advasep-7 (Biotium), a cyclodextrin that minimizes extraneous FM1-43 labeling 30 ...
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bioRxiv - Cell Biology 2022Quote: Calcium transients of cultured embryonic cardiomyocytes at DIV4-5 were measured after incubating myocytes with the calcium-sensitive fluorescence dye Fura2-AM (1 μM, Biotium, Cat: 50033-1) diluted in 0.02% Pluronic acid F-127/DMSO (Invitrogen ...