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Citations for Biotium Inc. :
301 - 350 of 396 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... the mouse brain sections were treated with TrueBlack® Lipofuscin Autofluorescence Quencher (Biotium, 23007, dilution 1:20) for 1 min ...
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bioRxiv - Developmental Biology 2019Quote: ... F-Actin was stained with Phalloidin conjugated with CF594 or CF568 (1:500) (Biotium #00044 and #00045, respectively).
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bioRxiv - Cell Biology 2021Quote: ... the chromatin was stained with RedDot™2 Far-Red Nuclear Stain (Biotium, 1:100 diluted in PBS), and the samples mounted using Vectashield Mounting Medium and analyzed on an FV1000 ...
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bioRxiv - Microbiology 2020Quote: ... wells were washed twice with fresh media before CAMHB containing the eDNA stain (EthHD-2 (1 μM; Biotium)) was added to the wells ...
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bioRxiv - Genomics 2020Quote: ... Fragmented RNA was incubated in low-input Biotinylation Solution (25 mM Hepes pH 7.5, 1 mM EDTA, 25% dimethylformamide, 16.4 μM MTS-Biotin (Biotium)) for 30 minutes in the dark at 25 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Four μL of each reaction product was mixed with 1 μL of loading dye containing Gel Red (Biotium), and visualized on a 1% agarose gel after 60 minutes at 100 V ...
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bioRxiv - Developmental Biology 2020Quote: ... separated on a 1% agarose gel and visualized with GelRed nucleic acid stain (cat #41001, Biotium, Fremont, CA) using a Syngene G:Box Chemi imager (Syngene ...
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bioRxiv - Cell Biology 2021Quote: ... JC-1 Mitochondrial Membrane Potential Detection Kit and MitoView™ Green were purchased from Biotium (Hayward, CA, USA). Mouse monoclonal anti-α-Tubulin-FITC antibody and anti-phospho-Histone H2A.X (Ser139 ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in RPMI 1640 medium and stained for 5 min with 1 μg/ml of WGA-CF594 (Biotium). Gametogenesis was induced by XA at the concentration indicated for each experiment ...
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bioRxiv - Neuroscience 2023Quote: ... their positivity for RedDot1 (Viability dye, Far-red DNA intercalating agent, Biotium, #40060-1, λEx/λEm (with DNA) = 662/694 nm) ...
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bioRxiv - Neuroscience 2023Quote: ... the mouse brain sections were dipped briefly in TrueBlack® Lipofuscin Autofluorescence Quencher (dilution 1:10, Biotium, 23007) for 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1 h at 37°C in mKRB supplemented with native coelenterazine (5 µM, BIOTIUM), and then incubated for 15 minutes in mKRB supplemented with either TPEN (500 µM ...
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bioRxiv - Synthetic Biology 2023Quote: ... Both treated cells and control cells were stained with wheat germ agglutinin (WGA) conjugates CF568 (Biotium 29077-1) to visualize the plasma membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then washed with annexin-binding buffer and additionally incubated with NucSpot Live 650 (1:1,000; Biotium) for 1 hr at 37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quality control was performed by running 1 μL of eluted DNA on a 1.0% agarose gel stained with GelRed (Biotium) and visually inspecting whether bands of high molecular weight DNA were visible ...
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bioRxiv - Neuroscience 2021Quote: ... we incubated one cochlea in HBSS containing annexin V-Alexa647 at a 1/25 dilution and CF488A-WGA (Biotium) at 20 μg/mL and the other cochlea in the same buffer supplemented with 100 μM benzamil for 25 min at RT in an orbital shaker protected from the light ...
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bioRxiv - Microbiology 2021Quote: ... The size of the amplified fragments was analyzed by 1%-agarose gel electrophoresis and DNA staining with GelRed (Biotium), and the correct insert’s nucleotide sequence was confirmed by automated DNA sequencing ...
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bioRxiv - Bioengineering 2022Quote: Frozen samples from saliva nuclease degradation assays were analysed on 1% (w/v) agarose gels stained with GelRed (Biotium) and visualised on a BioRAD Gel Doc EZ Imager with a UV transilluminator ...
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bioRxiv - Immunology 2022Quote: ... 10 μl of each sample were loaded into a 1% agarose gel with gel red (Biotium, Fremont, CA, USA) together with 2 μL 6x loading dye (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... The resulting PCR products were separated on 1% agarose gel and visualized using GelRed Nucleic Acid Gel Stain (Biotium) under UV illumination in GelDoc-It system (UVP) ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μl of the digested DNA was mixed with 2 μl 1:100 in DMSO diluted GelRed™ (Biotium) dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Cell Biology 2021Quote: ... 5 h) in 50 mM HEPES pH 7.8 containing 1 mM EDTA and stained with GelRed (Biotium, cat#41003). After washing the gel with 75 mM NaOH for 15 min ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were then run on a 1% agarose gel and visualised using GelRed Nucleic Acid Gel Stain (Biotium). Only well separated bands corresponding to the main PCR products obtained were cut out from the gel and PCR products were extracted from gel slices using GenElute Agarose Spin Columns (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μg of RNA were biotinylated in biotinylation buffer (10 mM Tris-HCl pH 7.4, 1 mM EDTA, 20 ng/μl MTSEA Biotin-XX (Biotium)) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were washed and incubated in secondary antibody solution with Goat anti-Rabbit Alexa 568 (Biotium, 1:500) and Goat anti-Mouse IgG1α Alexa 488 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Four μL of the ITS PCR product was mixed with 1 μL of loading dye containing Gel Red (Biotium), and visualized on a 1% agarose gel after 60 minutes at 100 V ...
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bioRxiv - Biophysics 2023Quote: ... the growth medium was removed and cells were incubated with 1 μM CellBrite™ NIR680 (Biotium, Fremont, CA, USA) for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were permeabilized with 0.5% Triton X-100 for 10 minutes and stained with CF647-phalloidin (1:40 v/v, Biotium) for 1 hour in the dark under gentle mixing ...
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bioRxiv - Cell Biology 2024Quote: ... The media was then replaced with 100µLper well supplemented F12-K medium with 500nM JF646 Halo-tag ligand and 1:1000 MembraneSteady-488nm (Biotium).
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bioRxiv - Cell Biology 2022Quote: ... infected MDMs were rinsed in PBS and incubated for 15 min at 37°C with a 1/1000 dilution of CellBrite Fix-640 (Biotium). Cells were rinsed twice with PBS and fixed in PFA 4% for confocal microscopy.
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA extractions were quantified with the Qubit fluorometer and quality was assessed with 1% agarose gels stained with GelRed (Biotium) and visualized with UV light.
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bioRxiv - Bioengineering 2020Quote: ... was used to dye the actin cytoskeleton alongside with CF488 anti-rabbit IgG antibody (Biotium, Fremont, USA, 1:500 dilution) in 0.5% w/v BSA 0.1% v/v Triton X-100 in PBS for 1h ...
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bioRxiv - Cell Biology 2021Quote: Viability of cardiac PCs and CAECs exposed to 1 μg/mL (5.8 nM) S protein or PBS vehicle was evaluated using the Viability/Cytotoxicity Assay Kit (Biotium #30002), according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were analyzed via agarose gel electrophoresis on a 1 % (w/v) agarose gel with 0.001 % GelGreen® Nucleic Acid Gel Dye (Biotium) (90 V ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were separated by electrophoresis in a 1 % TAE-agarose gel stained with GelRed nucleic acid gel stain (Biotium), gel-purified (QIAquick gel purification Kit ...
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bioRxiv - Cell Biology 2022Quote: ... at a 1:2000 dilution in PBS with 4% BSA was added and incubated for 30 min together with CF405M-Phalloidin (Biotium) at a 1:100 dilution to label F-actin ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of bacteria suspension was added to 250 μL of enhancer for Gram-negative Bacteria (Biotium San Francisco, US). PMAxx™ Dye was dissolved in H2O at 5mM and added to a bacterial solution at a final concentration of 25μM ...
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bioRxiv - Neuroscience 2020Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594- or CF488A-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated donkey anti-mouse IgG ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed in PBS for 5 minutes and incubated for 1 minute in TrueBlack lipofuscin autofluorescence quencher (Biotium #23007) in 70% ethanol ...
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bioRxiv - Zoology 2022Quote: ... Endosymbiont presence was detected by separation of PCR products on a 1% agarose gel stained with GelRed® (Biotium, Germany), and reactions were visualised under UV light ...
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bioRxiv - Cell Biology 2019Quote: ... freshly thawed cells were diluted at 1×106 cells/mL and CellBrite TM NIR790 Cytoplasmic Membrane Dye (Biotium, cat.30079) was added at the concentration of 1µM and incubated at 37°C for 20 minutes with frequent agitation ...
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bioRxiv - Genomics 2021Quote: ... cells were lysed directly in 1X RIPA buffer and blots were incubated with goat anti-mouse 680 (1:5000, cat #20253, Biotium). Fluorescent immunoreactivity was imaged on a LICOR Odyssey.
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bioRxiv - Microbiology 2022Quote: ... 20 μL of bacterial cultures were incubated with Wheat Germ Agglutinin Alexa Fluor 488 Conjugate (Biotium; Catalogue no. 29022–1) at a final concentration of 0.1 mg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... samples were labeled with an anti-Myosin 7A antibody (#25-6790 Proteus Biosciences, 1:400) and goat anti-rabbit CF568 (Biotium) secondary antibody ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Japan) for 2 h at room temperature and then stained with 1 μM Mito View Green solution (Biotium, Fremont, CA) and 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... then were incubated for 10 minutes at 21 °C in 1 μM 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM, Biotium) in PSS ...
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bioRxiv - Neuroscience 2023Quote: ... Hair cells were labeled with an anti-Myosin 7A primary antibody (#25-6790 Proteus Biosciences, 1:400) followed by application of goat anti-rabbit CF568 (Biotium) secondary antibody ...