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Citations for Biotium Inc. :
1 - 50 of 396 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... the cells were incubated in AnV buffer (1:175 dilution of the fluorescently tagged AnV (Biotium) in Hank’s balanced salt solution ...
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bioRxiv - Physiology 2021Quote: ... PS exposure was detected using CF 594–tagged Annexin V (AnV, Biotium, # 29011). To monitor Ca2+ and PS dynamics before stimulation ...
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bioRxiv - Biophysics 2020Quote: ... Human Transferrin – CF488A (Biotium) at 130 nM ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... ; Blots were washed 3 times in TBS/0.1% Tween and incubated with secondary IRD-tagged antibodies (CF770, Biotium) diluted 1:10,000 in Odyssey blocking buffer for 1 h at room temperature.
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bioRxiv - Microbiology 2024Quote: ... and 1/1000 Alexa FluorTM 488-conjugated cross-absorbed goat anti-human IgG (heavy chain specific) (Biotium, 20444). The plates were incubated for 30 min at 37°C in the dark ...
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bioRxiv - Microbiology 2024Quote: ... the secondary antibody 1/1000 Alexa Fluor 647TM-conjugated goat anti-human IgG (gamma chain specific) (Biotium, 20448) in PBS/1% BSA contained 1/2500 of VybrantTM DyeCycleTM Violet stain and 20 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... Labeling of protein carbonyl groups was obtained by overnight incubation of 20μg protein extracts with CF555DI hydrazide (Biotium, Fremont, CA, USA, #92165) at 4°C under medium agitation ...
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bioRxiv - Cell Biology 2019Quote: ... The Calbryte-590 loaded cells were then incubated in HBSS buffer containing 0.5 µg/mL CF 488-tagged-Annexin-V (Biotium, cat. 29011), a fluorescence PS probe ...
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bioRxiv - Neuroscience 2022Quote: ... Human transferrin-CF488A conjugate was purchased from Biotium (cat. #00081). Mouse monoclonal anti-GFAP (cat ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse and anti-human IgG coupled to CF488R (Biotium) or CF640R (Biotium ...
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bioRxiv - Microbiology 2023Quote: ... with either AccuGreen broad-range or high-sensitivity dsDNA detection kits (Biotium). To preserve mutant abundance ratios and reduce the propagation of PCR errors ...
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bioRxiv - Neuroscience 2022Quote: ... or 10 μM fluorescently labeled human transferrin (Tf488; Biotium, cat. #00081) for 4 h at 37 °C under gentle agitation ...
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bioRxiv - Genomics 2021Quote: ... We measured DNA concentrations with Accuclear ultra high sensitivity dsDNA quantification kit (Biotium) and normalized all samples at a concentration of 5ng/ μL ...
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bioRxiv - Molecular Biology 2021Quote: ... All libraries were quantified by the AccuGreen High Sensitivity dsDNA Quantitation Kit (Biotium, USA), and the size of the libraries was determined on the Agilent Bioanalyzer 2100 (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were quantified using the Accuclear Ultra High Sensitivity dsDNA Quantitative kit (Biotium) and then pooled in equimolar amounts ...
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bioRxiv - Immunology 2019Quote: ... and quantified with the Accuclear Ultra High Sensitivity dsDNA kit (Biotium, Cat. No. 31028). ∼10 ng of cDNA was stamped into a fresh 384-well plate (Eppendorf ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed using Fast Plus EvaGreen® qPCR master mix High ROX (Biotium). Primers for genes of interest and genes of reference (GAPDH and HPRT1 ...
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bioRxiv - Plant Biology 2023Quote: ... After DNA quantification using the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium, USA), bacterial and fungal libraries were constructed largely following our two-step PCR profiling protocol described earlier (Gfeller et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Autofluorescence quenching: Human sections were treated with TrueBlack Lipofuscin Autofluorescence Quencher (TLAQ) (Biotium) per manufactures instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by DNA quantification using the AccuGreen™ High Sensitivity dsDNA Quantitation kit (Biotium, 31066) on clear flat-bottom 96-well polystyrene plates (Corning® ...
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bioRxiv - Genomics 2021Quote: ... Final concentrations of the libraries were quantified with Accuclear ultra high sensitivity dsDNA quantification kit (Biotium) and fragment size distribution was estimated with an Agilent BioAnalyzer for a subset of 10 to 20 samples per plate ...
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bioRxiv - Microbiology 2022Quote: ... Cleaned barcoded 16S rRNA amplicons were quantified using AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium) on BioTek™ FLx800™ Microplate Fluorescence Reader ...
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bioRxiv - Neuroscience 2022Quote: ... Human neuronal cultures were fixed on coverslips in 4%PFA (catalog#22023, Biotium, Fremont, CA, USA) for 1h at RT and then blocked for 1.5h at RT in 0.1% Triton-X and 10% horse serum (catalog#H0146 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the concentration of the PCR products was quantified using the Accugreen High Sensitivity dsDNA Quantitation Kit (Biotium 31068). Sequencing libraries were then pooled equally and sequenced on a NextSeq500 Mid flow cell (150 bp single-end reads).
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bioRxiv - Bioengineering 2023Quote: Extracellular dsDNA release from spheroids was measured using AccuGreen™ High Sensitivity dsDNA Quantitation Kit (BIOTIUM 31066-T). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... explants were incubated with fluorescently conjugated human transferrin-594 (CF 594; 25 μg/mL; Biotium Fremont CA) for 40 minutes ...
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bioRxiv - Genetics 2020Quote: ... The PCR product’s concentration was quantified with Qubit (dsDNA High Sensitivity Assay) and its length was verified on a 2.5% agarose gel with GelRed (Biotium). Excess primers and dNTPs were removed by ExoSAP-IT (ThermoFisher ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA concentrations were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and diluted to 2 ng μL-1 using a Myra Liquid Handler (Bio Molecular Systems ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product’s concentration was quantified with Qubit (dsDNA High Sensitivity or Broad Range Assay) and its length was verified on a 2.5% agarose gel with GelRed (Biotium). Excess primers and dNTPs were removed by ExoSAP-IT (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the concentration of the second round PCR products was quantified using the Accugreen High Sensitivity dsDNA Quantitation Kit (Biotium 31068). These libraries were then normalized ...
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bioRxiv - Plant Biology 2022Quote: All the PCR products were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and subpooled by sample type ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Concentration of DNA in the sample was further estimated using the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium, Fremont CA, USA) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Wheat Germ Agglutinin (WGA) (1:1000, 29022-1, Biotium), and Non-muscle Myosin II A (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... goat-anti-mouse CF680 (1:500 dilution, Biotium, 20065-1) and goat-anti-mouse Cy3 (1:500 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... goat-anti-mouse CF680 (1:500 dilution, Biotium, 20065-1) and goat-anti-mouse Cy3 (1:500 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... we utilized fluorophore-conjugated forms of the lectin wheat germ agglutinin (WGA) (Biotium, 29022- 1, 29062-1, 29025-1) to outline all cellular membranes ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated Donkey anti-Sheep IgG (for ChAT, 1:250, #20191-1, Biotium). All antibodies were diluted in the blocking solution.
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bioRxiv - Neuroscience 2023Quote: ... along with 1% BSA and mixed 1:200 with RedDot (40060, Biotium). The samples were then isolated from debris by fluorescence -activated nuclei sorting (FANS) ...
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bioRxiv - Genomics 2021Quote: ... 1 × EvaGreen dye (Biotium) was added to the amplification mixture to monitor the PCR amplification ...
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bioRxiv - Cell Biology 2024Quote: ... + 1:1000 Enhancer (Biotium) and incubated for ∼10-15min before starting imaging ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 μL of aniline (diluted 1:10 in water) and 1 μL of 100 mM biocytin hydrazide (Biotium, 90060). The reaction proceeded while rotating at 4°C for 90 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with RedDot™1 Far-Red Nuclear Stain (1:200, Biotium) at room temperature for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... goat Anti-Guinea Pig IgG Polyclonal CF-488A (1:500, Biotium, cat#: 20017-1), cy5-AffiniPure Donkey Anti-Guinea Pig IgG H&L (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Phalloidin CF405 (1:200; Biotium) or MitoSpy™ NIR DiIC1 dye (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... Hoecsht 33342 (1:500; Biotium), and rhodamine-phalloidin (1:20 ...
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bioRxiv - Neuroscience 2022Quote: ... DAPI (1:2000; Biotium 40011), Hoechst (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 x EvaGreen dye (Biotium), 3 µl DNA template dilution (1×104 copies/reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 x EvaGreen dye (Biotium), 3 µl DNA template dilution (1×104 copies/reaction ...
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bioRxiv - Genetics 2022Quote: ... DAPI (Biotium#40043, 1:500) was used as a nuclear counterstain ...
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bioRxiv - Cell Biology 2021Quote: ... Phalloidin CF647 1:100 (Biotium) in PBS + 1% FBS ...