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Citations for Biotium Inc. :
251 - 300 of 666 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2024Quote: ... Amplicons were visualized on 2% agarose gels stained with GelRed Nucleic Acid Gel Stain (Biotium, Hayward, CA, USA). Positive amplicons were extracted from the gels and purified using the QIAquick gel extraction kit (Qiagen ...
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bioRxiv - Bioengineering 2024Quote: ... the mice were administered an intraperitoneal injection of D-luciferin (0.2 mL, 15 mg/mL; Biotium, Fremont, CA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... Wheat Germ Agglutinin (WGA) (1:1000, 29022-1, Biotium), and Non-muscle Myosin II A (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-mouse CF633 (1:500, Biotium #20250-1), and goat anti-rat AF647 (1:500 ...
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bioRxiv - Genetics 2022Quote: ... we performed PCR in 10 μl reactions using Apex Taq DNA polymerase and EvaGreen Dye (Biotium, Fremont, CA, USA) as the intercalating dye ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with wheat germ agglutinin (WGA) conjugated to horseradish peroxidase (HRP) (Biotium Inc. Hayward, CA, USA) at a dilution of 1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA concentrations were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and diluted to 2 ng μL-1 using a Myra Liquid Handler (Bio Molecular Systems ...
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bioRxiv - Neuroscience 2023Quote: ... floating sections or tissue-mounted slides were incubated with TrueBlack® Plus Lipofuscin Autofluorescence Quencher (Biotium, Fremont, CA, USA) in 1X phosphate buffer saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... washed three times in 1xTBS and incubated in a 1x solution of TrueBlack lipofuscin autofluoresence quencher (Biotium, Fremont, CA) in 70% ethanol for 2 min before rinsing in 1xTBS ...
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bioRxiv - Microbiology 2023Quote: ... DNA integrity was evaluated by electrophoresis in 0.8% (w/v) agarose gels stained with GelRed (Biotium, Fremont, CA, USA).
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bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
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bioRxiv - Bioengineering 2024Quote: ... the collected cells were stained with the Live-or-Dye 594/614 Fixable Viability Staining KitTM (Biotium, Fremont, CA) and then analyzed by a flow cytometer (BD LSRFortessa X20 ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated with 3% H2O2 for 15 minutes and true black (catalog #: 23007 Biotium; Fremont, CA, USA) for 1 minute to reduce auto-fluorescence ...
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bioRxiv - Genetics 2024Quote: ... Quantitative real-time PCR assays were performed on the Bio-Rad CFX96 instrument using Evagreen (Biotium, Freemont, CA, USA) with a procedure of 98 °C 3min ...
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bioRxiv - Neuroscience 2024Quote: ... Ha and PDa with similar densities were selected and incubated with 6 μM Fluo4-AM (Biotium, Fremont, CA, USA) in HBSS for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... goat-anti-mouse CF680 (1:500 dilution, Biotium, 20065-1) and goat-anti-mouse Cy3 (1:500 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... goat-anti-mouse CF680 (1:500 dilution, Biotium, 20065-1) and goat-anti-mouse Cy3 (1:500 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... goat-anti-mouse CF680 (1:500 dilution, Biotium, 20065-1).
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated for 45 sec at room temperature in a 1x solution of TrueBlack (Cat. #23007, Biotium, Fremont, CA) prepared in 70% ethanol ...
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bioRxiv - Neuroscience 2020Quote: ... USA) and the products were separated through electrophoresis on a 2% agarose gel stained with GelRed (Biotium, Fremont, CA, USA). The wild-type allele generated a single fragment of 460 bp and the mutant allele generated two fragments of 276 and 184 bp.
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bioRxiv - Evolutionary Biology 2021Quote: ... All reactions were visualized in a 1.5% Agarose gel containing 1X GelRed fluorescent nucleic acid dye (Biotium, Fremont, CA, USA). Positive samples were sequenced on an Applied Biosystems 3730XL DNA Analyzer at the University of Arizona Genetics Core.
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bioRxiv - Plant Biology 2022Quote: All the PCR products were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and subpooled by sample type ...
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bioRxiv - Microbiology 2024Quote: ... The washed cells were stained with 0.1 mg/ml CF®488A wheat germ agglutinin conjugate (Biotium, Fremont, CA, USA) in PBS for 5 min at room temperature and washed two more times with PBS buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... we utilized fluorophore-conjugated forms of the lectin wheat germ agglutinin (WGA) (Biotium, 29022- 1, 29062-1, 29025-1) to outline all cellular membranes ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed three times with PBST and incubated for one hour at 4°C with donkey anti-goat IgG-CF555 conjugated (Biotium-Fisher scientific, Cat: 50-196-4162), diluted 1:100 in PBST containing 0.1% BSA ...
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bioRxiv - Microbiology 2020Quote: ... The 10 µL qPCR reactions contained 1 µL of sample and 9 µL of master mix (0.25 mM of each primer, 1X Forget-Me-Not EvaGreen qPCR mix (Biotium, Fremont, CA), and 0.4 mg mL-1 BSA ...
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bioRxiv - Plant Biology 2022Quote: ... faded with 96% alcohol and processed after the addition of SynaptoRed C2 as stated by the manufacturer (Biotium, Fremont, CA, USA), following the methodology described by (55) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The fluorescence values of these reads were compared to those read from calcium standards made with the Buffer Kit for Calibration of Fluorescent Ca² Indicators (Biotium, 59100). To measure free magnesium concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Microbiology 2023Quote: To differentiate between viable and non-viable cells and viruses in qPCR, half of the aliquots mentioned above (i.e., the second set of triplicates) received a PMAxx dye treatment (Biotium, Fremont, CA). Aliquots of the biomass samples (230 μL ...
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bioRxiv - Bioengineering 2024Quote: The biocompatibility characterization of the cell-laden hydrogels was done by using a standard live/dead assay (Biotium, Fermont, CA, USA). The assay comprised fluorescent dyes Calcein-AM for staining live cells and ethidium homodimer III (EthD-III ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated Donkey anti-Sheep IgG (for ChAT, 1:250, #20191-1, Biotium). All antibodies were diluted in the blocking solution.
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bioRxiv - Genomics 2021Quote: ... 1 × EvaGreen dye (Biotium) was added to the amplification mixture to monitor the PCR amplification ...
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bioRxiv - Cell Biology 2024Quote: ... + 1:1000 Enhancer (Biotium) and incubated for ∼10-15min before starting imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... Evagreen (1:20 Biotium), and 1x ROX reference dye (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 μL of aniline (diluted 1:10 in water) and 1 μL of 100 mM biocytin hydrazide (Biotium, 90060). The reaction proceeded while rotating at 4°C for 90 minutes ...
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bioRxiv - Biophysics 2021Quote: ... The upstream handle was labeled at the 3’ end of the DNA strand by introducing biotin-16-dUTP (Biotium, Fremont, CA, USA) using the T4 DNA polymerase (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... Labeling of protein carbonyl groups was obtained by overnight incubation of 20μg protein extracts with CF555DI hydrazide (Biotium, Fremont, CA, USA, #92165) at 4°C under medium agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed 3 times for 5 min with PBS to remove unbound antibody and subsequently stained with CF660C labeled anti-rabbit antibody (20183, Biotium, Fremont, CA) diluted 1:150 in PBS containing 5% (v/v ...
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bioRxiv - Genetics 2020Quote: ... PCR product was examined by 1.5% (w/v) agarose 0.5x TBE buffer (45mM Tris-borate, 1mM EDTA) gel electrophoresis with GelGreen stain (Biotium Inc., Fremont, CA, USA).
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bioRxiv - Bioengineering 2020Quote: ... osteogenically induced MSCs were stained with 10 µM of the near infrared (NIR) dye CellBrite NIR680 Cytoplasmic Membrane Dye (Biotium, Fremont, CA) per manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... GloMelt (λEx = 468 nm λEm = 507 nm) and carboxyrhodamine (ROX; λEx = 588 nm λEm = 608 nm) dyes were purchased from Biotium (Fremont, CA). The PFAS analyzed are shown in Figure 1 ...
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bioRxiv - Neuroscience 2020Quote: ... samples were passively incubated for 48 hours at room temperature in eFLASH sample buffer.32 Rapid 24 hour immunostaining of whole hemispheres with polyclonal CF640R rabbit anti-RFP (Biotium, Fremont, CA) was carried out using the eFLASH protocol.32 The samples were again passively washed in PBSN three times for a minimum of 12 hours each before refractive index matching.
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bioRxiv - Neuroscience 2022Quote: ... The resulting cDNA was diluted five-fold and RT-qPCR was performed with Eva™ Green PCR Master Mix (Biotium, Fremont, CA) in a StepOne Plus Real-Time PCR Platform (Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Furimazine was purchased from Aobious (Gloucester, MA), ceolentrazine and coelentrazine analogues (coelenterazine-f, -h, -fcp, -hcp, -n, and -cp) were purchased from Biotium (Fremont, CA). Polyethyleneimine (PEI ...
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bioRxiv - Immunology 2024Quote: ... at the time of co-culture on day 7 followed by Live-or-Dye™ 405/545 viability stain (Biotium, Fremont, CA) on day 13 ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Concentration of DNA in the sample was further estimated using the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium, Fremont CA, USA) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: DRG neurons grown on coverslips as described above were first treated with a blocking solution consisting of sterile HBSS with 10 mM HEPES + 1% horse serum for 5 minutes at room temperature followed by staining with HBSS/HEPES + 70 mM Di-8-ANEPPS (Biotium, Fremont, CA) for 15 minutes room temperature and recovery in HBSS/HEPES + 10% horse serum for 5 minutes ...