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Citations for Biotium Inc. :
251 - 300 of 376 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... For mitochondria live imaging, ROs were incubated with 1X SPY555 vital DNA dye (Spirochrome, #SC201) over night and MitoView 650 (Biotium, #70075) at 200 nM for 30 min prior to imaging ...
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bioRxiv - Molecular Biology 2024Quote: 10 ng of bisulfite-converted DNA input were used in a 10 µL reaction with 2x Biotium Universal Probe qPCR Master Mix with ROX dye (Biotium #31044). Each reaction contained 500 nM of each primer and 200 nM of the probe (probe concentration was reduced to 100 nM for multiplex qPCR assays) ...
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bioRxiv - Immunology 2024Quote: ... urothelial sheets were stained with fluorescent dyes NucSpot650 and CellBrite405 (final concentration 1:100 in PBS as per manufacturers’ recommendations, Biotium, US) for 40 minutes ...
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bioRxiv - Bioengineering 2024Quote: Cytotoxicity was determined in the absence of HSV infection by treating cells with specified doses of candidate antivirals and using a combination of membrane permeable NucSpot Live 488 dye (Biotium 40081) and membrane impermeable NucSpot 594 dye (Biotium 41037) ...
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bioRxiv - Genomics 2022Quote: ... Total protein level of each lane was normalized by VersaBlot™ Total Protein Normalization Kits (Biotium Cat. # 33025).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... GloMelt (λEx = 468 nm λEm = 507 nm) and carboxyrhodamine (ROX; λEx = 588 nm λEm = 608 nm) dyes were purchased from Biotium (Fremont, CA). The PFAS analyzed are shown in Figure 1 ...
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bioRxiv - Immunology 2024Quote: ... at the time of co-culture on day 7 followed by Live-or-Dye™ 405/545 viability stain (Biotium, Fremont, CA) on day 13 ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of each fecal inoculum was mixed with 2.5 µL of 20 mM propidium monoazide (PMA) dye (Biotium, Fremont, CA, USA). The samples were then incubated in the dark for 5 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GloMelt (λEx = 468, λEm = 507 nm) and carboxyrhodamine (ROX; λEx = 588, λEm = 608 nm) dyes (CAT #33022-1) were purchased from Biotium (Fremont, CA). Bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng of bisulfite-converted DNA input were used in 10 µL qPCR reactions with 2x EvaGreen qPCR MasterMix with ROX dye (Biotium, Cat#31042). Each reaction contained 200 nM of both forward and reverse primers ...
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bioRxiv - Microbiology 2024Quote: The cytosolic pH of wild-type and mutant strains was measured using the pH-sensitive fluorescent dye BCECF [2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein] (Biotium; product 51011). The BCECF was added to saponin-isolated parasites by suspension (1×108 parasites/mL ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed 4x with PBS before staining with CellBrite® Green Cytoplasmic Membrane Dye as per manufacturer instructions (Biotium, #30021). Cells were washed 3x with PBS ...
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bioRxiv - Biophysics 2024Quote: ... eosinophils were incubated with low-toxicity CellBrite Steady Red cytoplasmic membrane dye (1:200 dilution, Ex/Em 562/579 nm, Cat. 30107-T, Biotium, CA, USA) at 37℃ for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibodies and secondary antibodies conjugated to CF568 (1:3000, Biotium). A few additional modifications were included to improve the specificity of labeling for dSTORM ...
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bioRxiv - Cell Biology 2022Quote: Calcium transients of cultured embryonic cardiomyocytes at DIV4-5 were measured after incubating myocytes with the calcium-sensitive fluorescence dye Fura2-AM (1 μM, Biotium, Cat: 50033-1) diluted in 0.02% Pluronic acid F-127/DMSO (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... A typical reaction contained 100 nM of each primer and either 150 nM fluorescent probe or lx EvaGreen dye (Biotium, Fremont, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... and DNA from membrane-compromised bacterial cells was removed by treatment of coral homogenates and tissue airbrushed blanks with the viability dye PMAxxTM (Biotium, Fremont, CA, USA). PMAxx is a DNA-intercalating agent that forms photo-induced crosslinks making the bound DNA inaccessible for downstream molecular applications ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... incubated in secondary antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 and 0.01% SDS for 1 hr ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl of gDNA was used as template in a 20 μl qPCR reaction containing 10 μl of 2x Fast-Plus EvaGreen Master Mix with low ROX dye (Biotium, Inc. via VWR, Germany), 10 pmol of each forward and reverse primer (Table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... 5 minutes each with PBS and incubated for 10 minutes at RT with a 1:200 dilution in PBS of the lipophilic dye CellBrite™ (Biotium. Cat. Nº: 30023). Finally ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were incubated in a Flexstation 3 for at least 60 minutes at 37°C in Ca5 dye plus 1.25 mM probenecid (Biotium #50027, Jomar Life Research, Australia) prior to the start of recording ...
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bioRxiv - Microbiology 2023Quote: ... and used in a qPCR using a ready-to-use mix containing a fluorescent-intercalating dye (EvaGreen® Fast qPCR Master Mix, Biotium, San Francisco, USA). After the amplification steps ...
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bioRxiv - Developmental Biology 2020Quote: ... Labeling of protein carbonyl groups was obtained by overnight incubation of 20μg protein extracts with CF555DI hydrazide (Biotium, Fremont, CA, USA, #92165) at 4°C under medium agitation ...
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bioRxiv - Cell Biology 2021Quote: Spermine fluorescent labeling with CF405M or CF594 dyes was performed using a Mix-n-Stain Small Ligand Labeling Kit (Biotium, Cat# 92362 and 92352, respectively) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... fluorescent secondary antibodies from Biotium, cat# 20308 ...
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bioRxiv - Immunology 2021Quote: ... stained with one-step blue protein gel stain (Biotium, ref. 21003), and by SEC-HPLC with an xbridge protein BEH SEC (Waters ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 4°C primary with antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 ...
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bioRxiv - Biophysics 2024Quote: ... The proteins were visualized by One-Step Blue Stain (Biotium, Fremont, CA). Bands of S protein were excised from the gel and processed as previously described27 ...
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bioRxiv - Neuroscience 2020Quote: The following secondary antibodies were used in this study: CF680-conjugated donkey anti-mouse IgG antibody (Biotium, 20819), Alexa-647-conjugated donkey anti-mouse IgG antibody (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in secondary antibody solution which contained anti-rabbit CF488 secondary antibody (1:100; Biotium), rhodamine phalloidin (1:50 ...
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bioRxiv - Cell Biology 2020Quote: ... CF488-conjugated anti-rat IgG antibody (Biotium).
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bioRxiv - Cell Biology 2021Quote: ... goat anti-rabbit secondary antibody (Biotium 20064); and goat anti-mouse secondary antibody (Invitrogen SA5-35521).
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bioRxiv - Immunology 2022Quote: ... secondary antibodies (488, 555, 633, 680 Biotium) and DAPI (D1306 ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were blocked for one hour and were incubated with primary antibodies diluted in TrueBlack WB Antibody Diluent (Biotium) supplemented with 0.2% Tween-20 (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... Coverslips were washed three times for 5 min with PBS to remove unbound antibody and subsequently stained with CF660C labeled anti-rabbit antibody (catalog no. 20183, Biotium) or anti-mouse antibody (catalog no ...
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bioRxiv - Cell Biology 2020Quote: ... antibody labeling kit Mix-n-Stain CF640R (Biotium); heme (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... or anti-mouse antibody (catalog no. 20815, Biotium) diluted 1:150 in PBS containing 5% [v/v] NGS for 1 h ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... antibody labeling kit Mix-n-Stain CF640R (Biotium), Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... secondary antibodies (Invitrogen, A21202; Biotium, 20171; Invitrogen, A32790) were applied at 1:500 dilution.
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bioRxiv - Neuroscience 2024Quote: ... Highly Cross-Adsorbed secondary antibody (CF488, Biotium, 20015) was added at 1:1000 in 2% BSA for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies were used as follows: CF 488-conjugated goat anti-mouse IgG (H+L) antibody (20302-1; Biotium, CA, USA), CF 555-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... Protein gels were transferred onto nitrocellulose membranes and subsequently stained with Ponceau S (Biotium) before blocking in 5% BSA (Sigma ...
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bioRxiv - Biochemistry 2022Quote: Thermal melt measurements were using a GloMelt Thermal Shift protein stability kit from Biotium on a Qiagen Rotor-gene Q qPCR instrument ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... protein expression of the FRHΔ100 plasmid was induced with 0.2 mM IPTG (Biotium, 10021), at 18°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... the gels were re-stained with One-Step Lumitein™ Protein Gel Stain (Biotium) as per manufacturer’s directions and imaged again as before.
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bioRxiv - Genetics 2023Quote: ... Total protein stain was then conducted by diluting CF568-labeled succinimidyl ester (Biotium, 92131) at 1:100,000 in water containing 0.1 M NaHCO3 and 25 mM Na2CO3 ...
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bioRxiv - Biophysics 2024Quote: ... double cysteine constructs of proteins were fluorescently labeled with Cy3B (Cytiva) and CF660R (Biotium), as donor and acceptor dyes ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed 3 times for 5 min with PBS to remove unbound antibody and subsequently stained with CF660C labeled anti-rabbit antibody (20183, Biotium, Fremont, CA) diluted 1:150 in PBS containing 5% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... tendons were washed six times in PBS and placed in secondary antibody solution containing fluorescent CF488 anti-mouse secondary antibody (1:200; Biotium, Fremont, CA), Hoecsht 33342 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and secondary antibodies: CF488A (1:2000; 20010, 20012 Biotium), CF555 (1:2000 ...