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Citations for Biotium Inc. :
201 - 250 of 376 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... [Ca2+]m was measured in cells loaded with Ca2+ specific dye Rhod2-AM (Biotium, Cat #50024) (1 μM ...
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bioRxiv - Cancer Biology 2020Quote: Cellular death was measured with Live-or-Dye™ 488/515 Fixable Staining Kit (Biotium, USA) as per as manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated RNA was separated on a 1% agarose gel (1XTBE) and visualized using GelRed dye (Biotium). Both inputs (corresponds to 5% ...
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bioRxiv - Systems Biology 2024Quote: ... The amplified DNA products were labeled with GelGreen® Nucleic Acid Gel Stain dye (Biotium # 41004) and visualized by ChemiDoc Imaging Systems (Bio-rad ...
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bioRxiv - Microbiology 2024Quote: ... bacterial cultures were treated with the membrane impermeable DNA dye ethidium homodimer-III (EthD-III; Biotium) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... sialic acid residues at the cell surface were oxidized using sodium meta-periodate (Thermo) and biotinylated with aminooxy-biotin (Biotium). Following quenching ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was denatured at 60C for ten minutes before being biotinylated by the addition of 5ug/ml biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: 10 mcg total RNA (extracted without exposure to DTT) was biotinylated by mixing with 1 mcg MTSEA-biotin-XX (Biotium) in 10 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was subjected to biotinylation (120 ug RNA, 20 mM HEPES pH 7.4, 12 ug MTSEA biotin-XX (Biotium Co) in 600ul final volume for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were run on a 2% agarose gel supplemented with gel green dye (Biotium, 41005-1). A subset of F0 samples was submitted to GeneWiz for Sanger sequencing and analyzed using SnapGene software (SnapGene).
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bioRxiv - Bioengineering 2024Quote: Samples were prepared by mixing SEC-purified CD4-Ig with 25X GloMelt™ Dye (Biotium, #99843-20uL) to reach a final concentration of 10 nM CD4-Ig and 2X dye ...
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bioRxiv - Physiology 2023Quote: ... and voltage (Vm)-sensitive dyes (RH237, 50 µL of 1 mg/mL in DMSO, Biotium, Hayward, CA) through the coronary perfusion ...
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bioRxiv - Microbiology 2024Quote: ... CF583R-conjugated donkey anti-rabbit IgG (Custom CF Dye, lot 23C0811, Biotium, 1:250, 4 μg/mL). To confirm that the fluorophore attached to the secondary antibody does not produce artifacts ...
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bioRxiv - Microbiology 2024Quote: ... CF583R-conjugated donkey anti-mouse IgG (Custom CF Dye, lot 23C1122, Biotium, 1:250, 4 μg/mL), CF583R-conjugated donkey anti-rabbit IgG (Custom CF Dye ...
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bioRxiv - Bioengineering 2024Quote: Live cell staining of 3T3 cells was conducted using CellBrite Steady Dye 550 diluted 1:1000 (Biotium) for membrane staining ...
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bioRxiv - Cancer Biology 2024Quote: ... HT1080 cells were labeled with CellBrite®™ Steady 488 membrane dye (Biotium® catalog #30106-T) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... or 50 nM DNA template (mntP riboswitch, Template 6, Supplementary Table 3) with randomly distributed internal biotin-11 dNMPs (AAT BioQuest/Biotium), 0.5 µM holoRNAP ...
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bioRxiv - Genetics 2021Quote: ... and an aliquot was used to verify size on an agarose gel stained with GelGreen dye (41005, Biotium). Final libraries underwent quality control verifications using a bioanalyzer and were sequenced on an Illumina NextSeq500.
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bioRxiv - Cell Biology 2021Quote: ... lenses were placed in staining solution containing fluorescent CF640 dye conjugated to wheat-germ agglutinin (Biotium, Fremont, CA) (1:500) ...
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bioRxiv - Microbiology 2022Quote: ... The viability of the bacteria was determined using qPCR after PMAxx™ Dye treatment (Biotium San Francisco, US) allowing specific DNA amplification of viable bacteria only ...
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bioRxiv - Plant Biology 2021Quote: ... protonemal cells were stained by the 5 µM of fluorescent dye SynaptoGreen C4 (equivalent to FM1-43, Biotium, USA ...
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bioRxiv - Plant Biology 2021Quote: ... Four μL of each reaction product was mixed with 1 μL of loading dye containing Gel Red (Biotium), and visualized on a 1% agarose gel after 60 minutes at 100 V ...
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bioRxiv - Immunology 2021Quote: Apoptotic DNA fragmentation was examined using 30064 CF™ 594 Dye TUNEL Assay Apoptosis Detection Kit (Biotium, Cedarlane) according to the manufacturer’s protocol ...
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... cells were rinsed and 1:1000 CellBrite Steady 650 membrane dye and 1:1000 kit enhancer (Biotium #30108) was added in Fluorobrite DMEM +10% FBS for 30 minutes at 37℃.
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bioRxiv - Molecular Biology 2023Quote: ... The success of amplification was monitored by 10% polyacrylamide gel electrophoresis and staining with GelGreen DNA dye (Biotium).
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bioRxiv - Neuroscience 2023Quote: ... their positivity for RedDot1 (Viability dye, Far-red DNA intercalating agent, Biotium, #40060-1, λEx/λEm (with DNA) = 662/694 nm) ...
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bioRxiv - Neuroscience 2024Quote: ... Each slice (n = 83) was injected with CF® 488A Dye Dextran (Biotium, Cat#80110; 2% solution, 10kD) using a 25 or 32 gauge Hamilton Syringe (Hamilton ...
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bioRxiv - Bioengineering 2024Quote: ... and the excess FM1-43 dye was neutralized using a quenching solution composed of 0.2 mM SCAS (Biotium) in L-15 and taken immediately for live imaging ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was incubated with goat anti-rabbit fluorescent IR dye at 680 nm (Biotium cat # 20193-1) and CF 800 goat anti-mouse secondary antibody (Biotium cat# 20831) ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR products were analysed by 10% polyacrylamide gel electrophoresis after staining with GelGreen® dye (Biotium) (all oligonucleotide used are listed in the Table S3).
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bioRxiv - Biophysics 2021Quote: ... The upstream handle was labeled at the 3’ end of the DNA strand by introducing biotin-16-dUTP (Biotium, Fremont, CA, USA) using the T4 DNA polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from the cell pellets using the hot-phenol procedure and followed by biotinylation with MTSEA-Biotin-XX (Biotium, USA, Fremont) as previously described (27 ...
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bioRxiv - Biophysics 2022Quote: ... The protein band was stained by One-Step Blue Protein Gel Stain (Biotium, Fremont, CA).
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bioRxiv - Genetics 2022Quote: ... we performed PCR in 10 μl reactions using Apex Taq DNA polymerase and EvaGreen Dye (Biotium, Fremont, CA, USA) as the intercalating dye ...
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bioRxiv - Cell Biology 2020Quote: ... was loaded by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator/dye Di-4-ANEPPS (10 µM; Biotium) for at least 30 min at room temperature (20–22 °C) ...
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bioRxiv - Microbiology 2022Quote: ... Four μL of the ITS PCR product was mixed with 1 μL of loading dye containing Gel Red (Biotium), and visualized on a 1% agarose gel after 60 minutes at 100 V ...
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bioRxiv - Bioengineering 2024Quote: ... the collected cells were stained with the Live-or-Dye 594/614 Fixable Viability Staining KitTM (Biotium, Fremont, CA) and then analyzed by a flow cytometer (BD LSRFortessa X20 ...
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bioRxiv - Genomics 2024Quote: Libraries were prepared using Accel NGS 2S Plus DNA library kit (IDT) following the manufacturer’s protocol with the following modification: 5% EvaGreen dye (Biotium) was added to the amplification mixture to monitor progress ...
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bioRxiv - Genomics 2024Quote: ... The same PCR setup can be used for a precedent qPCR by adding a qPCR dye as Evagreen (Biotium) to test for successful sample amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were analyzed via agarose gel electrophoresis on a 1 % (w/v) agarose gel with 0.001 % GelGreen® Nucleic Acid Gel Dye (Biotium) (90 V ...
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bioRxiv - Microbiology 2021Quote: ... A paired sample design was used where one DNA extraction tube was treated with the chemical propidium monoazide (PMAxx Dye, 20mM in H2O from Biotium) which prevents amplification of exDNA ...
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bioRxiv - Genetics 2020Quote: ... Samples were pulse centrifuged up to 1200 rpm and split in two, where one part (n = 230, PMA treated samples) was added PMA dye (Biotium, USA to a final concentration of 50 µM ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All reactions were visualized in a 1.5% Agarose gel containing 1X GelRed fluorescent nucleic acid dye (Biotium, Fremont, CA, USA). Positive samples were sequenced on an Applied Biosystems 3730XL DNA Analyzer at the University of Arizona Genetics Core.
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions of each of the cell lines were separately labeled with either one or both of ViaFluor 488 SE and ViaFluor 405 SE fluorescent dyes (Biotium) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cell growth quantification was performed using the ViaFluor 488 SE cell proliferation dye in minCMV reporter HEK293T cells (Biotium 30086) or CellTrace Far-Red proliferation dye in pEF reporter TC28a2 cells (Thermo C34564 ...
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bioRxiv - Neuroscience 2024Quote: ... to get rid of the excess maleimide dye and was subsequently conjugated to CF®568 succinimidyl ester (#92131, Biotium) via NHS reaction to increase the number of dyes per nanobody ...
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bioRxiv - Microbiology 2024Quote: ... All extracted DNA samples were qualitatively evaluated by mixing ∼2 μL of each sample with 1 μL of loading dye containing Gel Red (Biotium) and running it on a 0.7 % agarose gel for 50 minutes at 110 V ...
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bioRxiv - Genetics 2021Quote: ... The size selected libraries were verified for quality control by bioanalyzer as well as verification of the library size via visualization on an agarose gel stained with GelGreen dye (Biotium, 41005). Libraries were then sequenced on NovaSeq6000 Sprime Paired End (PE ...
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bioRxiv - Microbiology 2020Quote: ... at a final concentration of 20 μg/ml and incubated in the dark for 2 min before detection or with CellBrite™ Fix 488 Membrane Dye (Biotium) at a final concentration of 10X (from a 1:1000 dilution ...
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bioRxiv - Microbiology 2023Quote: To differentiate between viable and non-viable cells and viruses in qPCR, half of the aliquots mentioned above (i.e., the second set of triplicates) received a PMAxx dye treatment (Biotium, Fremont, CA). Aliquots of the biomass samples (230 μL ...