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Citations for Biotium Inc. :
251 - 300 of 304 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated at room temperature for 30 minutes in PBS containing LipidSpot reagent (1:1000; Biotium, Fremont, CA, USA), then washed 3x with PBS and mounted onto glass slides with Vectashield + DAPI.
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bioRxiv - Developmental Biology 2024Quote: ... and Wheat Germ Agglutinin conjugated with a 405 fluorophore (1:50 concentration, #29028-1 Biotium, San Francisco, CA, United States) were used to label PRC outer segments.
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bioRxiv - Microbiology 2020Quote: ... The 10 µL qPCR reactions contained 1 µL of sample and 9 µL of master mix (0.25 mM of each primer, 1X Forget-Me-Not EvaGreen qPCR mix (Biotium, Fremont, CA), and 0.4 mg mL-1 BSA ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and stained with a JC-1 Mitochondrial Membrane Potential Detection Kit using the manufacturers protocol (Biotium, Fremont, CA). The dye forms JC aggregates (orange ...
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bioRxiv - Microbiology 2023Quote: To differentiate between viable and non-viable cells and viruses in qPCR, half of the aliquots mentioned above (i.e., the second set of triplicates) received a PMAxx dye treatment (Biotium, Fremont, CA). Aliquots of the biomass samples (230 μL ...
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bioRxiv - Cell Biology 2024Quote: ... The fluorescence values of these reads were compared to those read from calcium standards made with the Buffer Kit for Calibration of Fluorescent Ca² Indicators (Biotium, 59100). To measure free magnesium concentrations ...
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bioRxiv - Plant Biology 2022Quote: ... faded with 96% alcohol and processed after the addition of SynaptoRed C2 as stated by the manufacturer (Biotium, Fremont, CA, USA), following the methodology described by (55) ...
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bioRxiv - Neuroscience 2023Quote: ... at room temperature for 60 min before incubation in PBS-T 0.3% with 3% NDS and CF405-conjugated streptavidin (1:1000; Biotium, CA, USA). After 90 min slices were washed in PBS (4x 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies were used as follows: CF 488-conjugated goat anti-mouse IgG (H+L) antibody (20302-1; Biotium, CA, USA), CF 555-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Bioengineering 2024Quote: The biocompatibility characterization of the cell-laden hydrogels was done by using a standard live/dead assay (Biotium, Fermont, CA, USA). The assay comprised fluorescent dyes Calcein-AM for staining live cells and ethidium homodimer III (EthD-III ...
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bioRxiv - Biophysics 2021Quote: ... The upstream handle was labeled at the 3’ end of the DNA strand by introducing biotin-16-dUTP (Biotium, Fremont, CA, USA) using the T4 DNA polymerase (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... Labeling of protein carbonyl groups was obtained by overnight incubation of 20μg protein extracts with CF555DI hydrazide (Biotium, Fremont, CA, USA, #92165) at 4°C under medium agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed 3 times for 5 min with PBS to remove unbound antibody and subsequently stained with CF660C labeled anti-rabbit antibody (20183, Biotium, Fremont, CA) diluted 1:150 in PBS containing 5% (v/v ...
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bioRxiv - Genetics 2020Quote: ... PCR product was examined by 1.5% (w/v) agarose 0.5x TBE buffer (45mM Tris-borate, 1mM EDTA) gel electrophoresis with GelGreen stain (Biotium Inc., Fremont, CA, USA).
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bioRxiv - Bioengineering 2020Quote: ... osteogenically induced MSCs were stained with 10 µM of the near infrared (NIR) dye CellBrite NIR680 Cytoplasmic Membrane Dye (Biotium, Fremont, CA) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The resultant triplicate PCR products for each sample were pooled and quantified using a 1% agarose gel stained with GelRed (Biotium, CA, USA). Quantified pooled triplicates were then combined in equimolar concentrations based on gel quantification to create an amplicon library ...
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bioRxiv - Cell Biology 2022Quote: ... tendons were washed six times in PBS and placed in secondary antibody solution containing fluorescent CF488 anti-mouse secondary antibody (1:200; Biotium, Fremont, CA), Hoecsht 33342 (1:500 ...
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bioRxiv - Biophysics 2022Quote: For imaging with WGA-CF680 the sample was incubated for 10 min with 1:10.000 diluted WGA-CF680 (29029-1, Biotium, Fremont, CA, USA) in 100 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were stained with 7 μM Calcofluor White for 20 minutes and 50 μg/mL of Concanavalin A (both obtained from Biotium, Fremont CA) for 30 minutes ...
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bioRxiv - Pathology 2020Quote: ... PCSK9 promoter-driven firefly luciferase activity and control renilla luciferase activity were measured using the Firefly & Renilla Luciferase Single Tube Assay Kit (#30081-1; Biotium, Fremont, CA) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... GloMelt (λEx = 468 nm λEm = 507 nm) and carboxyrhodamine (ROX; λEx = 588 nm λEm = 608 nm) dyes were purchased from Biotium (Fremont, CA). The PFAS analyzed are shown in Figure 1 ...
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bioRxiv - Neuroscience 2020Quote: ... samples were passively incubated for 48 hours at room temperature in eFLASH sample buffer.32 Rapid 24 hour immunostaining of whole hemispheres with polyclonal CF640R rabbit anti-RFP (Biotium, Fremont, CA) was carried out using the eFLASH protocol.32 The samples were again passively washed in PBSN three times for a minimum of 12 hours each before refractive index matching.
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bioRxiv - Neuroscience 2023Quote: DRG neurons grown on coverslips as described above were first treated with a blocking solution consisting of sterile HBSS with 10 mM HEPES + 1% horse serum for 5 minutes at room temperature followed by staining with HBSS/HEPES + 70 mM Di-8-ANEPPS (Biotium, Fremont, CA) for 15 minutes room temperature and recovery in HBSS/HEPES + 10% horse serum for 5 minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Concentration of DNA in the sample was further estimated using the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium, Fremont CA, USA) following manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... 1 mL of each fecal inoculum was mixed with 2.5 µL of 20 mM propidium monoazide (PMA) dye (Biotium, Fremont, CA, USA). The samples were then incubated in the dark for 5 min ...
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bioRxiv - Physiology 2023Quote: ... preparations were loaded with Ca2+ (Rhod-2 AM, 50 µL of 1 mg/mL in DMSO + 10% pluronic acid, Biotium, Hayward, CA) and voltage (Vm)-sensitive dyes (RH237 ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting cDNA was diluted five-fold and RT-qPCR was performed with Eva™ Green PCR Master Mix (Biotium, Fremont, CA) in a StepOne Plus Real-Time PCR Platform (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... at the time of co-culture on day 7 followed by Live-or-Dye™ 405/545 viability stain (Biotium, Fremont, CA) on day 13 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were separated by electrophoresis through a 2% agarose gel in 1× TBE and stained with GelRed (Biotium, Hayward, CA, USA). To confirm the sequence of each band ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GloMelt (λEx = 468, λEm = 507 nm) and carboxyrhodamine (ROX; λEx = 588, λEm = 608 nm) dyes (CAT #33022-1) were purchased from Biotium (Fremont, CA). Bovine serum albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Furimazine was purchased from Aobious (Gloucester, MA), ceolentrazine and coelentrazine analogues (coelenterazine-f, -h, -fcp, -hcp, -n, and -cp) were purchased from Biotium (Fremont, CA). Polyethyleneimine (PEI ...
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bioRxiv - Biophysics 2024Quote: ... eosinophils were incubated with low-toxicity CellBrite Steady Red cytoplasmic membrane dye (1:200 dilution, Ex/Em 562/579 nm, Cat. 30107-T, Biotium, CA, USA) at 37℃ for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... auto-fluorescent signal arising from endogenous lipofuscin was quenched with a 30-second exposure to TrueBlack® Lipofuscin Autofluorescence Quencher (Biotium, Fremont, CA) and washed with 1X-TBS prior to mounting ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed with PBS three times for 20 minutes and then incubated with commercial dye-conjugated secondary antibodies diluted in blocking solution (AF647-conjugates from Jackson ImmunoResearch Laboratories, West Grove, PA, and CF568-conjugates from Biotium, Fremont, CA) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... the cell aggregates were processed and imaged as described in the whole-mount immunofluorescence methods using Streptavidin (29072, Biotium, 1:500, CA, USA) and a DAPI (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Mix-n-Stain CF Dye Antibody Labeling Kits (CF-680, #92282, and CF-770, #92285) were purchased from Biotium (San Francisco, CA, USA).
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bioRxiv - Immunology 2020Quote: ... Unconjugated primary antibodies CD23 and IgM were conjugated with Mix-n-Stain fluorescent protein tandem dyes antibody labeling kit for APC-CF750T (Biotium, Fremont, CA, USA) and Mix-n-Stain cf dye antibody labeling kit for CF405M (Biotium ...
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bioRxiv - Cell Biology 2023Quote: ... by IP3R-mediated ER Ca2+ release were measured in cells loaded with membrane-permeant AM ester form of Fluo-3 (Fluo-3-AM) (Biotium, Fremont, CA, USA) and Fura-Red ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s protocol after a couple of days and CRISPR/Cas9 induced editing efficiency was analyzed by PCR and separation of amplicon on 2% agarose gel containing 1:10.000 GelRed nucleic acid gel stain (41003, Biotium Inc., Fremont, CA, USA). Amplicons were purified by MinElute Gel extraction kit (28606 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were CF680- or CF770-conjugated anti-mouse and anti-rabbit IgG antibodies (1:5000, #20067 and #20077, Biotium, Hayward, CA, USA). Antibodies were diluted in Blocking One (Nacalai Tesque) ...
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bioRxiv - Neuroscience 2024Quote: ... multi-shank neural probe (NeuroNexus A4×4-3mm-100-125-177-A16, Ann Arbor, MI, USA) pre-coated with DiI (Biotium, Fremont, CA, USA) for fluorescent detection of recording sites was advanced dorsoventrally at 5 µm/s through the IC using a hydraulic micropositioner (Model 650 ...
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bioRxiv - Microbiology 2023Quote: ... and DNA from membrane-compromised bacterial cells was removed by treatment of coral homogenates and tissue airbrushed blanks with the viability dye PMAxxTM (Biotium, Fremont, CA, USA). PMAxx is a DNA-intercalating agent that forms photo-induced crosslinks making the bound DNA inaccessible for downstream molecular applications ...
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bioRxiv - Bioengineering 2023Quote: ... These cross-sectioned samples were washed twice with PBS to remove the culture media before being incubated in a live/dead assay staining solution (obtained from Biotium, Hayward, CA, USA) for a duration of 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... A typical reaction contained 100 nM of each primer and either 150 nM fluorescent probe or lx EvaGreen dye (Biotium, Fremont, CA, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... Approximately 5 µL of each PCR product was screened on 1.5% agarose gels stained with GelRed (Biotium, Fremont, CA, USA, Cat #41002-1). Each locus was then categorized based on the amplification profile ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated with 3 μg/mL of CF®640R-conjugated mouse anti-human CD47 Ab (clone B6H12.2, cat. no. BNC400437-100, Biotium, Fremont, CA, USA) and 1× Hoechst 33342 Ready Flow™ Reagent (cat ...
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bioRxiv - Cancer Biology 2024Quote: ... Residual NaIO4 was removed with three washes of labelling buffer and cells were subsequently labelled with 5 mM biocytin hydrazide (Biotium, Fremont, CA, USA) in labeling buffer at 4°C with gentle rocking for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... The PCRs were performed using a Corbett Rotor-Gene 6000 Real-Time Thermocycler (Corbett Research Pty Ltd. Sydney, Australia) and Eva-Green™ (Biotium Hayward, CA) in a final volume of 20 µL ...
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bioRxiv - Bioengineering 2024Quote: ... A colorimetric assay using XTT (sodium 3’-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) was used in the form of a two-reagent kit (XTT, Biotium, Fermont, CA, USA). The working solution was prepared immediately before use as per manufacturer’s instructions by mixing 25 µL Activation Reagent with 5 mL XTT Solution ...