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Citations for Biotium Inc. :
151 - 188 of 188 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... at a 1:2000 dilution in PBS with 4% BSA was added and incubated for 30 min together with CF405M-Phalloidin (Biotium) at a 1:100 dilution to label F-actin ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated at 4°C for 4 h with CF488-conjugated anti-mouse IgG (1:2000) and CF568-conjugated anti-rabbit IgG (1:2000) antibodies (Biotium) diluted in PBS containing 2% FBS as secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... the inserts were transferred to a fresh 24-well plate with black walls containing 500 μl of 4 μM Calcein AM (Biotium) in mHBSS per well ...
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bioRxiv - Immunology 2019Quote: ... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were then incubated with 10 kDa CF640R-labeled dextran (100 ug/mL, Biotium #80115) for 15 minutes at 37°C in the presence of DMSO or SAR405 ...
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bioRxiv - Biochemistry 2020Quote: ... 16 xg at 4°C and the pellet resuspended and incubated in WGA488 stain (Biotium, 5 μg/mL, diluted in HBSS) for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were incubated in a Flexstation 3 for at least 60 minutes at 37°C in Ca5 dye plus 1.25 mM probenecid (Biotium #50027, Jomar Life Research, Australia) prior to the start of recording ...
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bioRxiv - Cell Biology 2023Quote: ... antibodies were used at 1μg/mL overnight at 4°C and were subsequently detected with STORM-appropriate secondary antibodies (Biotium; 20836 and 20811). STORM acquisition was facilitated with an N-STORM system (Nikon Instruments ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... multi-shank neural probe (NeuroNexus A4×4-3mm-100-125-177-A16, Ann Arbor, MI, USA) pre-coated with DiI (Biotium, Fremont, CA, USA) for fluorescent detection of recording sites was advanced dorsoventrally at 5 µm/s through the IC using a hydraulic micropositioner (Model 650 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... the mouse brain sections were treated with TrueBlack® Lipofuscin Autofluorescence Quencher (Biotium, 23007, dilution 1:10) for 1 min ...
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bioRxiv - Microbiology 2023Quote: ... The first 10 reactions were run on a 1% agarose gel stained with 0.1% GelRed (Biotium, USA) and visualised under a UV transilluminator to ensure amplicons were the required size ...
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bioRxiv - Biochemistry 2020Quote: ... BRET signals were recorded from microsomes (each ∼10 µg) in the presence of 5 µM coelenterazine (Biotium Corp.) using the Cytation 5 image reader (BioTek Instruments) ...
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bioRxiv - Molecular Biology 2021Quote: ... the sample was incubated for 10 min with 1:5000 diluted WGA-CF680 (29029-1, Biotium, Fremont, CA) in 100mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... BRET signals were recorded from microsomes (each ∼10 μg) in the presence of 5 µM coelenterazine (Biotium Corp.) using the Cytation 5 image reader (BioTek Instruments ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was denatured at 60°C for 10 min and biotinylated using 5 µg/mL biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... The success of amplification was monitored by 10% polyacrylamide gel electrophoresis and staining with GelGreen DNA dye (Biotium).
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bioRxiv - Neuroscience 2023Quote: ... the mouse brain sections were dipped briefly in TrueBlack® Lipofuscin Autofluorescence Quencher (dilution 1:10, Biotium, 23007) for 1 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Fragmented RNA was incubated in Biotinylation Solution (25 mM HEPES; pH 7.4, 10 mM EDTA; pH 8.0, 50 µg MTS-Biotin (Biotium)) for 30 min in the dark at 24°C and 800 rpm ...
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bioRxiv - Genomics 2023Quote: ... rinsed in PBS for 10 min at RT and blocked using blocking buffer (Fish Gelatin blocking agent, BIOTIUM) in the dark for 1 hr ...
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bioRxiv - Genetics 2022Quote: ... we performed PCR in 10 μl reactions using Apex Taq DNA polymerase and EvaGreen Dye (Biotium, Fremont, CA, USA) as the intercalating dye ...
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bioRxiv - Biophysics 2021Quote: ... The sample was then incubated for 10 min with 1:5,000 diluted WGA-CF680 (catalog no. 29029-1, Biotium) in 100 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... 10 μl of each sample were loaded into a 1% agarose gel with gel red (Biotium, Fremont, CA, USA) together with 2 μL 6x loading dye (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μg of RNA were biotinylated in biotinylation buffer (10 mM Tris-HCl pH 7.4, 1 mM EDTA, 20 ng/μl MTSEA Biotin-XX (Biotium)) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were permeabilized with 0.5% Triton X-100 for 10 minutes and stained with CF647-phalloidin (1:40 v/v, Biotium) for 1 hour in the dark under gentle mixing ...
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bioRxiv - Biophysics 2023Quote: ... the sample was then incubated for 10 min with 400 ng ml−1 WGA-CF680 (catalog no. 29029-1, Biotium) in 100 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... The membranes were placed in an opaque gel box and incubated with blocking buffer (PBST + 10% Fish gelatin (PBS 10X, pH 7.2, Thermo Fisher Scientific, Cat-No. 70-013-032; Biotium 10X Fish Gelatin Blocking Agent ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then re-hydrated in PBS for 10 minutes before blocking with TrueBlack® IF blocking buffer (Biotium #23012B) for 10 minutes ...
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bioRxiv - Genomics 2021Quote: ... 100 µL 2x copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction buffer was added to cells to append a fluorophore reporter (2x concentrations: 10 µM CF488A picolyl azide (Biotium #92187), 2 mM CuSO4 (Sigma C8027) ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 μL of aniline (diluted 1:10 in water) and 1 μL of 100 mM biocytin hydrazide (Biotium, 90060). The reaction proceeded while rotating at 4°C for 90 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 1 h at RT with the secondary antibodies HRP goat anti-mouse (1:200 in 1% BSA-PBS) followed by 10 min amplification reaction with tyramide for the detection of Ngn3 (Tyramide Amplification Kid, 33003, Biotium, USA), or Alexa 594 donkey anti-rabbit for the detection of Pomc/Npy (1:1000 in 1% BSA-PBS ...
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bioRxiv - Microbiology 2023Quote: ... All tubes were incubated in the dark at room temperature for 10 mins before being exposed to light to cross-link PMA molecules using the PMA-LiteTM LED Photolysis Device (Biotium Inc.). DNA was then isolated from all samples using either the DNeasy PowerSoil Kit (Qiagen ...
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bioRxiv - Bioengineering 2020Quote: ... osteogenically induced MSCs were stained with 10 µM of the near infrared (NIR) dye CellBrite NIR680 Cytoplasmic Membrane Dye (Biotium, Fremont, CA) per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: For imaging with WGA-CF680 the sample was incubated for 10 min with 1:10.000 diluted WGA-CF680 (29029-1, Biotium, Fremont, CA, USA) in 100 mM Tris ...
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bioRxiv - Physiology 2023Quote: ... preparations were loaded with Ca2+ (Rhod-2 AM, 50 µL of 1 mg/mL in DMSO + 10% pluronic acid, Biotium, Hayward, CA) and voltage (Vm)-sensitive dyes (RH237 ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl of gDNA was used as template in a 20 μl qPCR reaction containing 10 μl of 2x Fast-Plus EvaGreen Master Mix with low ROX dye (Biotium, Inc. via VWR, Germany), 10 pmol of each forward and reverse primer (Table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... 5 minutes each with PBS and incubated for 10 minutes at RT with a 1:200 dilution in PBS of the lipophilic dye CellBrite™ (Biotium. Cat. Nº: 30023). Finally ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Live stain cells were washed twice using 100 µl ASW and then incubated for 10 min in 50 µg/ml WGA-CF633 conjugate (Biotium, Cat No #29024-1) in ASW ...