Labshake search
Citations for Biotium Inc. :
101 - 150 of 188 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: Cells were fixed with 4% paraformaldehyde and stained with Phalloidin-CF594 conjugate (Biotium), DAPI (Invitrogen) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were replaced with medium containing 5 µM NucView 488 caspase 3 substrate (Biotium Inc., #10403). After 30 min incubation at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... a reagent cocktail comprised of Hoechst 33342 and NucView 488 Caspase-3 reporter (Biotium, Cat# 30029) was added to each well for final concentrations of 1 μg/mL and 1 μM ...
-
bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was analyzed using the NucView® 488 caspase-3 assay kit (Biotium, Cat# 10402-T) which contains NucView® 488 Caspase-3 substrate ...
-
bioRxiv - Systems Biology 2020Quote: ... 1 μl 10 mg/ml MTSEA-XX-Biotin (Biotium). The reaction was incubated at room temperature for 30 to 60 min in the dark ...
-
bioRxiv - Biochemistry 2023Quote: ... gels were placed in 10 μM CF568-BCN (Biotium) for 2 hours ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2016) using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
-
bioRxiv - Cell Biology 2021Quote: Staining of vacuoles: SynaptoRed™ C2 (Equivalent to FM®4-64) (Cat. # 70021, Biotium) was added to the growth media of cells (1 × 10^6 cells/ml ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2015) using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
-
bioRxiv - Cell Biology 2021Quote: ... and 4 uL of 1 mM of SGC5 (Cat. number 70057, Biotium Inc., Fremont, CA) was injected into the AC of an enucleated mouse eye ...
-
bioRxiv - Genomics 2023Quote: ... 64 using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were incubated for 20 hours at 4°C with anti-IgG (CF640R, Biotium Cat# 20177 ...
-
bioRxiv - Immunology 2023Quote: ... and contrasted with 10 µM CF tyramide dye (Biotium, USA). The following primary antibodies were used at the consequence and with indicated dilutions and CF dyes ...
-
bioRxiv - Microbiology 2022Quote: ... samples were placed on a microscope slide with 0.8% agarose gel containing 5 μM Bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)) (Biotium) and 0.75 μM permeability reporter PI (MP Biomedicals) ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... ; Blots were washed 3 times in TBS/0.1% Tween and incubated with secondary IRD-tagged antibodies (CF770, Biotium) diluted 1:10,000 in Odyssey blocking buffer for 1 h at room temperature.
-
bioRxiv - Neuroscience 2022Quote: ... Human neuronal cultures were fixed on coverslips in 4%PFA (catalog#22023, Biotium, Fremont, CA, USA) for 1h at RT and then blocked for 1.5h at RT in 0.1% Triton-X and 10% horse serum (catalog#H0146 ...
-
bioRxiv - Biophysics 2023Quote: ... we fixed the samples using a fixative buffer composed of 4% Paraformaldehyde (PFA, Biotium, Freemont, CA) supplemented with 2.5% of Glutaraldehyde (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2023Quote: ... 40 μg of RNA was biotinylated with 4 μg of MTSEA Biotin XX (Biotium Cat# 90066). The biotinylated RNA was isolated by binding to 80 μl of a Dynabeads MyOne Streptavidin C1 bead suspension (Invitrogen Cat# 65001 ...
-
bioRxiv - Neuroscience 2022Quote: ... or 10 μM fluorescently labeled human transferrin (Tf488; Biotium, cat. #00081) for 4 h at 37 °C under gentle agitation ...
-
bioRxiv - Neuroscience 2020Quote: ... and nuclear stain (DAPI; 10 ug/mL; Biotium, Fremont, CA, #40043) for 15m at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... clone 4G10 (10 μg/ml) conjugated with CF 647 dye (Biotium) in PBS with 0.1% BSA for 1 hour at RT ...
-
bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
-
bioRxiv - Neuroscience 2019Quote: ... then diluted to 6x) and run on agarose gels (1-2%) with 4% GelRed (Biotium, California, USA).
-
Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 4°C primary with antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 ...
-
bioRxiv - Bioengineering 2021Quote: ... the cells were fixed with 4% PFA and stained with CF532 Wheat Germ Agglutinin (WGA) Conjugates (Biotium) and DAPI and imaged under a confocal microscope (Leica SP8) ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were pre-incubated with Fura-2·AM or Fluo-4·AM (30 minutes, 1 μM, Biotium) at 37°C with 5% CO2 ...
-
bioRxiv - Biophysics 2023Quote: Conduction velocity (CV) was quantified by optical voltage mapping using di-4 ANEPPS (15 μM, Biotium #61010) as previously described.28 Briefly ...
-
bioRxiv - Biophysics 2021Quote: ... blocked with 10% FBS and incubated for 30 seconds with TrueBlack (Biotium) to reduce ECM autofluorescence ...
-
bioRxiv - Biochemistry 2021Quote: ... The mixture was biotinylated with 10 µg of MTSEA biotin-XX (Biotium) in 100 mM HEPES/pH 7.5 and 10 mM EDTA for 30 min at 25°C in dark with gentle agitation and the biotinylated RNA was recovered by ethanol precipitation ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The eBlock™ integrities were individually verified by electrophoretic analysis on 3% agarose-TBE gels stained with GelRed® (Biotium).
-
bioRxiv - Cell Biology 2023Quote: ... PCR products were separated on a 1.5-2% agarose gel (7-01P02-R, BioConcept) dissolved in TAE buffer (3-07F03-I, BioConcept) and visualized using GelRed Nucleic Acid Stain (Cat# 41003, Biotium). Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested on day 5 and the proportion of apoptotic cells was assessed using the NucView 488 Caspase-3 Assay Kit (Biotium) according to the manufacturer’s instructions and an Attune NxT flow cytometer (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... at room temperature for 60 min before incubation in PBS-T 0.3% with 3% NDS and CF405-conjugated streptavidin (1:1000; Biotium, CA, USA). After 90 min slices were washed in PBS (4x 10 min ...
-
bioRxiv - Immunology 2023Quote: ... Samples were washed 3 times with PBS and incubated for 30 seconds with TrueBlack following the manufacturer’s instructions (Biotium, Cat# 23007). Samples were washed with PBS ...
-
bioRxiv - Bioengineering 2023Quote: ... Samples were then incubated with primary antibodies in 2% BSA on a gentle shaker at room temperature for 3 days followed by three times of 1X PBS washing and incubation of secondary antibodies and CF® Phalloidin (Biotium) staining for 1 hour at room temperature in the dark ...
-
bioRxiv - Neuroscience 2022Quote: ... and dH20 and incubated for 10 minutes in 1:1000 aqueous DAPI (Biotium) prior to mounting with Fluoromount G (SouthernBiotech) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Neuroscience 2020Quote: ... postsynaptic nicotinic receptors (nAChRs) were labeled using α-Bungarotoxin (BTX) conjugated with CF405 (4 μg/mL; Biotium; cat ##00002) for 45 min in PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... 4 μl of the digested DNA was mixed with 2 μl 1:100 in DMSO diluted GelRed™ (Biotium) dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
-
bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Biochemistry 2023Quote: ... DNA binding experiments using plasmid-length substrates were performed under the same conditions except with 100 ng DNA per reaction and were run on 0.8% agarose gel at 4 °C and post-stained with GelRed (Biotium).
-
bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
-
bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
-
bioRxiv - Biophysics 2021Quote: ... The upstream handle was labeled at the 3’ end of the DNA strand by introducing biotin-16-dUTP (Biotium, Fremont, CA, USA) using the T4 DNA polymerase (New England Biolabs ...
-
bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR was performed in 10 µL reactions with EvaGreen Mastermix (Biotium) using kit-specified reaction parameters in a Quant Studio 5 Real-Time PCR machine (ThermoFisher Scientific) ...
-
bioRxiv - Cell Biology 2021Quote: ... we examined the diffusion of a fluorescence conjugated dextran (CF488A-dextran 10 kDa, Biotium) over time using fluorescence confocal microscopy (LSM980 ...
-
bioRxiv - Neuroscience 2020Quote: ... in combination with nuclear staining using DAPI (10 ug/mL; Biotium, Fremont, CA, #40043). Images were captured using a 20x objective ...
-
bioRxiv - Microbiology 2020Quote: ... Gels were post-stained for 10 minutes in 0.5X TB Buffer with GelGreen (Biotium) and rinsed with sterile water before imaging with UV on an EZ Dock Imager (Bio-Rad) ...
-
bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 10% FCS in PBS with either DAPI (1 μM; Biotium, #40043) or DRAQ7 (300 nM ...