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Citations for Biotium Inc. :
151 - 200 of 1841 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Samples on day 7 post-inoculum were incubated with PMA before being treated with a blue LED photolysis device (Biotium, San Francisco), as described above ...
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bioRxiv - Physiology 2024Quote: ... cells were loaded with 1 μM Ca2+-specific dye Rhod2-AM (50024, Biotium, Fremont, CA) that preferentially localizes in mitochondria ...
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bioRxiv - Plant Biology 2024Quote: Calcofluor White solution: 5 mM Calcofluor White (Biotium, Cat. No. 29067, Fremont, CA, USA) to a final concentration of 50 µM in water.
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bioRxiv - Bioengineering 2024Quote: ... A typical reaction contained 100 nM of each primer and either 150 nM fluorescent probe or lx EvaGreen dye (Biotium, Fremont, CA, USA).
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bioRxiv - Biophysics 2024Quote: ... CF®568 succinimidyl ester (CF568-NHS) was purchased from Biotium (Fremont, CA). Dulbecco’s modified eagle medium (DMEM ...
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bioRxiv - Developmental Biology 2024Quote: ... and 488 Tyramide Conjugate (Biotium Inc, Cat. No. 92171) followed by DAPI staining with 1:20000 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... the STAT3 and peptide mixtures were added to 1X GlomeltTM (Biotium #33021-1, Fremont, California) a dye that binds hydrophobic residues and fluoresces upon protein denaturation due to an increase in heat ...
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bioRxiv - Microbiology 2024Quote: ... respectively (Biotium, #92213, #92214, #92218), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 0.6 µM EvaGreen (Biotium), 0.2 U of Hot Start Taq Polymerase (Sibenzyme ...
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bioRxiv - Microbiology 2024Quote: ... and 1/1000 Alexa FluorTM 488-conjugated cross-absorbed goat anti-human IgG (heavy chain specific) (Biotium, 20444). The plates were incubated for 30 min at 37°C in the dark ...
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bioRxiv - Microbiology 2024Quote: ... the secondary antibody 1/1000 Alexa Fluor 647TM-conjugated goat anti-human IgG (gamma chain specific) (Biotium, 20448) in PBS/1% BSA contained 1/2500 of VybrantTM DyeCycleTM Violet stain and 20 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... The quality of the extracted DNA was assessed by agarose gel electrophoresis (1.5% agarose gel, GelRed; Biotium, California, USA). The DNA concentration was quantified using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... 6-diamidino-2-phenylindole (DAPI, Biotium) and were examined with a Spinning Disk Carl Zeiss Axio Observer Z1 microscope using 10x magnification or with a Plan Apo 63x Ph3-NA1.4 –DIC HCII Oil immersion objective ...
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bioRxiv - Physiology 2024Quote: ... the membranes were blocked in 1X fish gelatin blocking buffer (Biotium Inc., CA, USA) for 1 h at room temperature and then incubated overnight at 4°C in chicken-anti-Hsd11b2 (Genetel ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Wheat Germ Agglutinin (CF®770) was purchased from Biotium (Hayward, CA, USA). Tissue culture treated black ...
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bioRxiv - Cell Biology 2024Quote: ... cell membrane was stained in live cells with CellBrite Fix 640 Membrane Labeling Kit (Biotium). The cells were then fixed in 2% paraformaldehyde and 0.1% glutaraldehyde (nacalai tesque ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Approximately 5 µL of each PCR product was screened on 1.5% agarose gels stained with GelRed (Biotium, Fremont, CA, USA, Cat #41002-1). Each locus was then categorized based on the amplification profile ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR was performed using Fast EvaGreen qPCR Master Mix (Biotium) using the forward and reverse primers listed in Dataset EV5 in the 7300 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... and endogenous binding sites were blocked using 1:10 fish gelatin blocking agent (Biotium, Cat# 22010) diluted in TBST for one hour ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was incubated with goat anti-rabbit fluorescent IR dye at 680 nm (Biotium cat # 20193-1) and CF 800 goat anti-mouse secondary antibody (Biotium cat# 20831) ...
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bioRxiv - Genomics 2024Quote: ... the integrity of the gDNA was verified through electrophoresis in 1% agarose gels stained with GelRed® Nucleic Acid Gel Stain (Biotium, USA), allowing visualization of high molecular weight DNA and detection of any degradation ...
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bioRxiv - Developmental Biology 2024Quote: ... and CF 800 goat anti-mouse secondary antibody (Biotium cat# 20831). The membranes were imaged under the LI-COR Odyssey scanner.
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bioRxiv - Microbiology 2024Quote: ... 130 nM CF488A in N-hydroxysuccinimide (NHS) ester (Biotium) was mixed with 2 μg anti-NGF antibodies in 1 mL PBS under stirring for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... sections were mounted in EverBrite TrueBlack Hardset Mounting Medium (Biotium, 23018) and visualized using a Leica STELLARIS 5 microscope with a 40x objective.
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibody solution was prepared in blocking buffer (CF488A or CF633A anti-mouse or anti-rabbit antibodies, Biotium cat# 20020 and 20124, 1:800). Once again ...
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bioRxiv - Neuroscience 2024Quote: ... (5%; Biotium cat: 23007) for 60 s followed by a 3×5 min rinse in 1M PBS prior to incubation with primary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was performed using 96-well plates containing 10 μL reactions composed of 5 μL of master mix (Biotium FastEvaGreen 2x ...
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bioRxiv - Cell Biology 2024Quote: ... CF 555-conjugated goat anti-mouse IgG (H+L) antibody (20231-1; Biotium, CA, USA), CF 488-conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... CF 488-conjugated goat anti-rabbit IgG (H+L) antibody (20019-1; Biotium, CA, USA), CF 555-conjugated goat anti-rabbit IgG (H+L ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... antibody labeling kit Mix-n-Stain CF640R (Biotium), Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... CF 555-conjugated goat anti-rabbit IgG (H+L) antibody (20232-1; Biotium, CA, USA).
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bioRxiv - Developmental Biology 2024Quote: ... Reb blood cell autofluorescence was quenched with TrueBlack Lipofuscin Autofluorescence Quencher (Biotium #2300) as per the manufacturer’s instructions then washed 2x in 1X PBS ...
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bioRxiv - Cell Biology 2024Quote: ... We detected apoptosis using the NucView488 reagent (Biotium, 10402), which is a Caspase-3/7 enzyme substrate that emits green fluorescence upon apoptotic cleavage ...
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bioRxiv - Genetics 2024Quote: ... Purified IKKε antibody was labeled with PE (Biotium, catalog number 92299). For intracellular staining ...
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bioRxiv - Genomics 2024Quote: ... The anti-MKI67IP and anti-SON antibodies were directly labelled with CF-594 and CF-640R fluorophores using Mix-n-Stain™ CF® Dye Antibody Labeling Kits (Biotium cat #92256 and #92258) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Libraries were prepared using Accel NGS 2S Plus DNA library kit (IDT) following the manufacturer’s protocol with the following modification: 5% EvaGreen dye (Biotium) was added to the amplification mixture to monitor progress ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with TrueBlack (Biotium, Cat; 23007) for 30 seconds ...
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bioRxiv - Microbiology 2024Quote: ... CF583R-conjugated donkey anti-rabbit IgG (Custom CF Dye, lot 23C0811, Biotium, 1:250, 4 μg/mL). To confirm that the fluorophore attached to the secondary antibody does not produce artifacts ...
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bioRxiv - Immunology 2024Quote: ... and contrasted with 10 µM CF tyramide dye (Biotium, USA). The following primary antibodies were used at the consequence and with indicated dilutions and CF dyes ...
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bioRxiv - Microbiology 2024Quote: ... Real-time PCR reactions were performed using RT2 Forget-Me-NotTM EvaGreen qPCR Mastermix (Biotium, CA, USA) on a CFX Connect Real-Time-PCR-System (BioRad) ...
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bioRxiv - Microbiology 2024Quote: ... or CF568 succinimidyl ester (NHS) (Biotium, 92131) dissolved in 2 µL dry DMSO (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Hoechst and wheat-germ agglutinin (WGA)-CF488 or WGA-CF561 (Biotium) in HBSS were added for 15 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... or RedDot2 (Biotium).
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bioRxiv - Microbiology 2024Quote: ... CF583R-conjugated donkey anti-mouse IgG (Custom CF Dye, lot 23C1122, Biotium, 1:250, 4 μg/mL), CF583R-conjugated donkey anti-rabbit IgG (Custom CF Dye ...
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bioRxiv - Molecular Biology 2024Quote: ... Then, cells were resuspended in PI Staining Solution (3.8 mM sodium citrate, 50 μg/mL PI (Biotium, USA) and 40 μg/mL RNase A (Zymo Research ...
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bioRxiv - Neuroscience 2024Quote: ... Retinae were then mounted in Drop-n-Stain EverBriteTM Mounting Medium (Biotium #23008) onto slides and imaged using Zeiss Axio Imager Z1 fluorescence microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... 1x106 cells/ml cells were stained with Live-or-Dye™ fixable dead cell dyes (1:1,000 dilution, cat#32016, Biotium) at RT for 30 min ...
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bioRxiv - Bioengineering 2024Quote: ... and donkey anti-goat IgG-Alexa Fluor 647 (1:400) respectively for 1 hour at room temperature followed by DAPI (BIOTIUM: 40043) staining ...
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bioRxiv - Bioengineering 2024Quote: Cell viability analysis was conducted utilizing the LIVE/DEAD Viability/Cytotoxicity Kit (Biotium, Fremont, CA, USA). For cell viability and growth assessments ...
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bioRxiv - Biophysics 2024Quote: ... The proteins were visualized by One-Step Blue Stain (Biotium, Fremont, CA). Bands of S protein were excised from the gel and processed as previously described27 ...