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Citations for Biotium Inc. :
451 - 500 of 1841 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Lipofuscin autofluorescence was quenched using TrueBlack® Lipofuscin quencher (Biotium) 1:20 in 70% ethanol for at least 30 seconds followed by three vigorous water washes ...
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bioRxiv - Immunology 2023Quote: ... was applied and coverslips were fixed with CoverGrip Coverslip Sealant (Biotium). Images were acquired using a Nikon Eclipse Ni-E microscope and processed with ImageJ software.
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bioRxiv - Biophysics 2023Quote: ... we fixed the samples using a fixative buffer composed of 4% Paraformaldehyde (PFA, Biotium, Freemont, CA) supplemented with 2.5% of Glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Gel red was from Biotium (Fremont, CA). Taq DNA polymerase was from Syd Labs (Hopkinton ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in TrueBlack (Biotium, 23007) for 3-5 minutes in order to mask confounding lipofuscin autofluorescence throughout the section ...
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bioRxiv - Neuroscience 2023Quote: ... incubated with TrueBlack Lipofuscin Autofluorescence Quencher (Biotium, 23007) for 10 seconds at room temperature to eliminate confounding lipofuscin autofluorescence present in the tissue ...
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bioRxiv - Plant Biology 2023Quote: ... total DNA was visualized on a 1% agarose gel in 1X TBE and GelRed 3x (Biotium) and the approximate length was estimated by comparing to a 100bp GeneRuler DNA ladder (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... All platereader experiments were performed with 20 μL volumes and the reactions were further supplemented with 2% BSA and 0.25x EvaGreen (Biotium, stock: 20x in H2O) in an optical ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were evaluated by electrophoresis on a 1% agarose gels containing 50 µM GelRed (Biotium) for 1 h at 110 V ...
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bioRxiv - Neuroscience 2023Quote: ... donkey anti-guinea pig 647 secondary antibody (Biotium, 20837, 1:1000), and DAPI (Biovision ...
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bioRxiv - Neuroscience 2023Quote: ... and labeled with Live-or-dye 750/777 stain (Biotium, 32008, 1:1000) in cold PBS for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylation of the mRNA was done using the MTSEA-biotin-XX reagent (Biotium 90066). A 5 mg/ml solution was prepared in N,NDimethylformamide (DMF ...
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bioRxiv - Cell Biology 2023Quote: ... while donkey anti-rabbit CF680 (Biotium catalog: 20418-50UL) and donkey anti-mouse CF583R (Biotium catalog ...
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bioRxiv - Cell Biology 2023Quote: ... followed by either donkey anti-mouse CF583R (Biotium catalog: 20794-500UL) for samples loaded with labeled fibrils ...
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bioRxiv - Biophysics 2023Quote: Conduction velocity (CV) was quantified by optical voltage mapping using di-4 ANEPPS (15 μM, Biotium #61010) as previously described.28 Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... samples were counterstained with DAPI (Cat. #40043, Biotium, USA).
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bioRxiv - Genetics 2023Quote: ... The PCR products were verified using 3.5% agarose gel (in 100 ml of TAE add 3.5 g Pronadisa Agarose D1 Low EEO and 3.5 ul Gel Red Biotium Solution) and electrophoresed using Mupid-exU submarine type electrophoresis system ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s protocol after a couple of days and CRISPR/Cas9 induced editing efficiency was analyzed by PCR and separation of amplicon on 2% agarose gel containing 1:10.000 GelRed nucleic acid gel stain (41003, Biotium Inc., Fremont, CA, USA). Amplicons were purified by MinElute Gel extraction kit (28606 ...
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bioRxiv - Microbiology 2023Quote: ... exposed to light in a PMA-Lite™ LED photolysis device (Biotium, Fremont, CA, USA) for light-induced crosslinking of PMA to dsDNA for 15 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... to obtain single-cell suspensions.68,86 Cells were stained with a far-red cell membrane-permeant nuclear dye (RedDot™1, Biotium) to specifically select live cells and DAPI to exclude dead cells ...
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bioRxiv - Microbiology 2023Quote: ... DNA integrity was evaluated by electrophoresis in 0.8% (w/v) agarose gels stained with GelRed (Biotium, Fremont, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with TrueBlack (23007, Biotium) for 1 min and then washed three times in TBS.
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bioRxiv - Cancer Biology 2023Quote: ... then washed with PBS five times and incubated overnight with Alexa Fluor 568 - conjugated anti-rabbit secondary antibodies (1:500, Biotium cat#20098) and DAPI dye ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG, 1:1000, Biotium; CF594-conjugated donkey anti-guinea pig IgG ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG ...
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bioRxiv - Immunology 2023Quote: ... lipofuscin autofluorescence was quenched by treatment with 1x TrueBlack stain (Biotium 23007) in 70% ethanol for 30 seconds followed by 3 washes in PBS ...
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bioRxiv - Physiology 2023Quote: ... the heart was injected with 1 ml of Rhod2-AM (30 µl of 1 mg/ml Rhod2-AM stock solution + 30 µl Pluronic F-127 + 940 µl Tyrode’s solution, Thermo Fischer Scientific R1244 and Biotium 59055, respectively) over a period of 3-5 minutes and followed by a second 5-minute washout period ...
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bioRxiv - Molecular Biology 2023Quote: ... one mg of eluted protein at a concentration of 50-150 µM was incubated with a 2× molar excess of CF660R (Biotium) or Cy3B (Cytiva ...
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bioRxiv - Synthetic Biology 2023Quote: ... The eBlock™ integrities were individually verified by electrophoretic analysis on 3% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Molecular Biology 2023Quote: ... and 0.6 μl of 1 mg/ml Wheat Germ Agglutinin (WGA) conjugated with CF®405S (Biotium). Cells were stained for 10 min at RT on a rotating mixer while covered with aluminium foil ...
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bioRxiv - Bioengineering 2023Quote: ... the Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Biotium, UK) was used according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were subsequently washed twice and stained with 1x Trueblack (Biotium; 23007) for 1 minute ...
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bioRxiv - Microbiology 2023Quote: ... dsRNA genome segments stained with GelRed (Biotium), and visualized at Odyssey Fc Imager (LI-COR Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... DNA was stained with 0.1% GelRed (Biotium) and examined at 20× magnification with an Olympus fluorescence microscope ...
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bioRxiv - Cell Biology 2023Quote: ... cells were exposed to 10 μM SynaptoRed™ C2 (Equivalent to FM®4-64; Biotium; 70027) in Stimulation Buffer consisting of 31.5 mM NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... After DNA quantification using the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium, USA), bacterial and fungal libraries were constructed largely following our two-step PCR profiling protocol described earlier (Gfeller et al. ...
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bioRxiv - Plant Biology 2023Quote: ... The amplified products were run on a 1% agarose gel (Duchefa, Netherlands) stained with GelRed® (Biotium, San Francisco, USA) and visualized under ultraviolet light.
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bioRxiv - Synthetic Biology 2023Quote: ... BigBlock assemblies and PCR products were analyzed by electrophoresis on 1-2% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Pathology 2023Quote: ... and dapi or NucSpot Live 488 (Biotium) were used for nuclear staining ...
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bioRxiv - Neuroscience 2023Quote: ... along with Lipidspot 488 (Biotium, 70065-T) and imaged for 24 hours.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were incubated in a Flexstation 3 for at least 60 minutes at 37°C in Ca5 dye plus 1.25 mM probenecid (Biotium #50027, Jomar Life Research, Australia) prior to the start of recording ...
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bioRxiv - Neuroscience 2023Quote: ... washed and then treated with TrueBlack lipofuscin autofluorescence quencher (Biotium, product number 23007) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Cellular mitochondrial mass and potential were determined using commercially available probes MitoView Green (MTV-G; Biotium, CA, USA) and Mitotracker Deep Red (MTDR ...
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Antigen Geometry Tunes Mast Cell Signaling Through Distinct FcεRI Aggregation and Structural ChangesbioRxiv - Biophysics 2023Quote: ... and CF640R- (Biotium #92108) labeled IgE lots were prepared using NHS Ester conjugation ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using EvaGreen (Biotium, Catalog no: 89138-982) and the Biorad CFX Connect Real-time PCR system ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with Hoechst 33342 (Biotium; 40046) at 1:10,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Cell boundaries were stained with Phalloidin cf532 in PBS (1:500, Biotium, 00051). Samples were mounted in ProLong Glass Antifade mounting medium (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were mounted in EverBrite TrueBlack® Hardset Mounting Medium (Biotium: 23017). Slides were imaged using Leica Bmi8 Thunder Imager microscope.