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Citations for Biotium Inc. :
701 - 750 of 1841 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... stained worms were resuspended in 15 μl of EverBrite Mounting Medium (Biotium) and mounted on slides ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were treated with Membrite Fix 640/660 membrane dye (Biotium) following manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... CellBrite(R) Red Cytoplasmic Membrane Dye was purchased from Biotium (CA, USA). PBS (pH 7.4 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were separated by electrophoresis through a 2% agarose gel in 1× TBE and stained with GelRed (Biotium, Hayward, CA, USA). To confirm the sequence of each band ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GloMelt (λEx = 468, λEm = 507 nm) and carboxyrhodamine (ROX; λEx = 588, λEm = 608 nm) dyes (CAT #33022-1) were purchased from Biotium (Fremont, CA). Bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... or when assaying synaptic puncta engulfment with TrueBlack Lipofusin Autofluorescence Quencher (according to manufacturer instructions, Biotium, 23007). Isotype controls (Abcam and Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... treated with TrueBlack® (Biotium, cat ...
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bioRxiv - Neuroscience 2023Quote: ... all sections were incubated with 1× TrueBlack Lipofuscin Autofluorescence Quencher (Biotium) in 70% ethanol for 30 seconds at room temperature before or after the staining process.
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bioRxiv - Neuroscience 2023Quote: ... TrueBlack Lipofuscin Autofluorescence Quencher (Biotium, 23007), Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... AM1-43 (Biotium, Fremont, CA) was diluted in sterile PBS and injected subcutaneously near the convergence of the wing and back ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were then treated TrueBlack Lipofuscin Autofluorescence Quencher (cat. #23007, Biotium), and incubated in Hoeschst for 10 minutes @RT ...
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bioRxiv - Neuroscience 2023Quote: ... along with 1% BSA and mixed 1:200 with RedDot (40060, Biotium). The samples were then isolated from debris by fluorescence -activated nuclei sorting (FANS) ...
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bioRxiv - Molecular Biology 2023Quote: Viability of CP and CAEC exposed to hemin or vehicle was evaluated using a Viability/Cytotoxicity Assay Kit (Biotium), according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... Fragmented RNA was incubated in Biotinylation Solution (25 mM HEPES; pH 7.4, 10 mM EDTA; pH 8.0, 50 µg MTS-Biotin (Biotium)) for 30 min in the dark at 24°C and 800 rpm ...
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bioRxiv - Molecular Biology 2023Quote: TUNEL assay was used to detect cell apoptosis using the CF605R TUNEL Assay Apoptosis Detection Kit (Biotium) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were blocked for 1 hour at room temperature with 1x Fish Gelatin Blocking Agent (Biotium, #22010) in wash buffer ...
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bioRxiv - Plant Biology 2023Quote: ... 1 x EvaGreen Dye (Biotium, Inc.) and 0.3 U Taq DNA polymerase (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... Copy number qPCR was performed using Forget-Me-Not qPCR Master Mix (Biotium) on a QuantStudio5 system.
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed in PBS and incubated with the following secondary antibodies (1:500, Biotium) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product’s concentration was quantified with Qubit (dsDNA High Sensitivity or Broad Range Assay) and its length was verified on a 2.5% agarose gel with GelRed (Biotium). Excess primers and dNTPs were removed by ExoSAP-IT (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... stained with CF488A conjugated Wheat germ agglutinin (Biotium, 2μg/mL) and Hoechst33342 (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were stained with CF633 (Biotium, USA) fluorescently labeled antibodies at 8 µg/ml in 1 % bovine serum albumin in PBS supplemented with 0.1 % Tween-20 for 1–3 h at room temperature in the dark ...
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bioRxiv - Bioengineering 2023Quote: Organoids were assayed for cell viability using a live/dead reagent kit (Biotium, Catalog #30002-T). Please see Sup ...
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bioRxiv - Neuroscience 2023Quote: ... goat Anti-Guinea Pig IgG Polyclonal CF-488A (1:500, Biotium, cat#: 20017-1), cy5-AffiniPure Donkey Anti-Guinea Pig IgG H&L (1:500 ...
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bioRxiv - Pathology 2023Quote: ... CF488 conjugated-donkey anti-rabbit IgG (Biotium, Hayward, CA, USA) and CF568 conjugated-donkey anti-mouse IgG (Biotium ...
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bioRxiv - Pathology 2023Quote: ... and CF568 conjugated-donkey anti-mouse IgG (Biotium, Hayward) were used as secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was denatured at 60C for ten minutes before being biotinylated by the addition of 5ug/ml biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Physiology 2023Quote: ... followed by donkey anti-rat CF750 (2 µg/mL, 20857, Biotium). Slides were imaged with the Slideview VS200 (Olympus ...
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bioRxiv - Genetics 2023Quote: ... The gel was stained with 0.1 uL Gel Red (Biotium, CA, USA) visualized under UV illumination at 300 nm using the Enduro GDS Touch Imaging System (Labnet International ...
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bioRxiv - Microbiology 2023Quote: ... and DNA from membrane-compromised bacterial cells was removed by treatment of coral homogenates and tissue airbrushed blanks with the viability dye PMAxxTM (Biotium, Fremont, CA, USA). PMAxx is a DNA-intercalating agent that forms photo-induced crosslinks making the bound DNA inaccessible for downstream molecular applications ...
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bioRxiv - Microbiology 2023Quote: ... Photoactivation was performed by using the PMA-LiteTM LED Photolysis Device (Biotium) with the exposure time set to 30 min ...
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bioRxiv - Genomics 2023Quote: ... The DNA samples were quantified before fragmentation reactions using Qubit and an AccuGreen Broad Range dsDNA Quantitation Kit (Biotium).
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bioRxiv - Genomics 2023Quote: ... cells were washed with DPBS and lysed directly in Qiazol reagent using a sterile miniature cell scraper (Biotium). Cell lysate was collected and stored for subsequent RNA processing and analysis.
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bioRxiv - Genomics 2023Quote: ... rinsed in PBS for 10 min at RT and blocked using blocking buffer (Fish Gelatin blocking agent, BIOTIUM) in the dark for 1 hr ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in DMEM 1% penicillin/streptomycin with 5 nM MitoView 533 (Biotium, #70055). Plates were incubated at 37°C for 30 min protected from light ...
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bioRxiv - Immunology 2023Quote: ... qPCR was performed with the Fast EvaGreen qPCR Master Mix (Biotium) and measured on a ABI7300 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... 2.5X EvaGreen (Biotium, Fremont, CA), 0.02 U/µl Phusion polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... 1.5X EvaGreen® (Biotium), 4% DMSO (Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... or Resazurin Cell Viability Assay Kit (Biotium #30025-1). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... and the assay was performed using the Griess Reagent Kit (Biotium) following the guidelines as provided in the kit ...
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bioRxiv - Microbiology 2023Quote: ... and used in a qPCR using a ready-to-use mix containing a fluorescent-intercalating dye (EvaGreen® Fast qPCR Master Mix, Biotium, San Francisco, USA). After the amplification steps ...
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bioRxiv - Microbiology 2023Quote: ... 1X (Biotium, Fremont, CA, USA) as per manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were additionally stained with membrane marker CellBrite (dilution 1:200, Biotium, Fremont, CA, USA) and actin marker Phalloidin (dilution 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... sections were incubated in blocking solution containing Phallodin-CF680R (1:50, Biotium #00048) for 20 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Calcein AM (Biotium, Fremont ...
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bioRxiv - Developmental Biology 2023Quote: ... and renilla (Biotium 300821) luciferase assays according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... centrifuged for 10 min at ∼13,000×g and then 50 µl of the clear supernatant lysate was used separately in firefly (Biotium #30085-1) and renilla (Biotium 300821 ...
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bioRxiv - Cell Biology 2023Quote: ... and Hoechst 33342 (1:500; Biotium) to visualize specific proteins (i.e ...
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bioRxiv - Cell Biology 2023Quote: ... and Cellbrite Steady 550 with Cellbrite Enhancer reagent (Biotium, 30107-T) by first making a 10X solution by diluting the three reagents 1:100 in R10 medium ...