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Citations for Biotium Inc. :
701 - 750 of 1910 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with TrueBlack (23007, Biotium) for 1 min and then washed three times in TBS.
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bioRxiv - Cancer Biology 2023Quote: ... then washed with PBS five times and incubated overnight with Alexa Fluor 568 - conjugated anti-rabbit secondary antibodies (1:500, Biotium cat#20098) and DAPI dye ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG, 1:1000, Biotium; CF594-conjugated donkey anti-guinea pig IgG ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG ...
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bioRxiv - Immunology 2023Quote: ... lipofuscin autofluorescence was quenched by treatment with 1x TrueBlack stain (Biotium 23007) in 70% ethanol for 30 seconds followed by 3 washes in PBS ...
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bioRxiv - Physiology 2023Quote: ... the heart was injected with 1 ml of Rhod2-AM (30 µl of 1 mg/ml Rhod2-AM stock solution + 30 µl Pluronic F-127 + 940 µl Tyrode’s solution, Thermo Fischer Scientific R1244 and Biotium 59055, respectively) over a period of 3-5 minutes and followed by a second 5-minute washout period ...
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bioRxiv - Molecular Biology 2023Quote: ... one mg of eluted protein at a concentration of 50-150 µM was incubated with a 2× molar excess of CF660R (Biotium) or Cy3B (Cytiva ...
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bioRxiv - Synthetic Biology 2023Quote: ... The eBlock™ integrities were individually verified by electrophoretic analysis on 3% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Molecular Biology 2023Quote: ... and 0.6 μl of 1 mg/ml Wheat Germ Agglutinin (WGA) conjugated with CF®405S (Biotium). Cells were stained for 10 min at RT on a rotating mixer while covered with aluminium foil ...
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bioRxiv - Bioengineering 2023Quote: ... the Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Biotium, UK) was used according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were subsequently washed twice and stained with 1x Trueblack (Biotium; 23007) for 1 minute ...
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bioRxiv - Microbiology 2023Quote: ... dsRNA genome segments stained with GelRed (Biotium), and visualized at Odyssey Fc Imager (LI-COR Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... DNA was stained with 0.1% GelRed (Biotium) and examined at 20× magnification with an Olympus fluorescence microscope ...
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bioRxiv - Cell Biology 2023Quote: ... cells were exposed to 10 μM SynaptoRed™ C2 (Equivalent to FM®4-64; Biotium; 70027) in Stimulation Buffer consisting of 31.5 mM NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... After DNA quantification using the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium, USA), bacterial and fungal libraries were constructed largely following our two-step PCR profiling protocol described earlier (Gfeller et al. ...
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bioRxiv - Plant Biology 2023Quote: ... The amplified products were run on a 1% agarose gel (Duchefa, Netherlands) stained with GelRed® (Biotium, San Francisco, USA) and visualized under ultraviolet light.
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bioRxiv - Synthetic Biology 2023Quote: ... BigBlock assemblies and PCR products were analyzed by electrophoresis on 1-2% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Pathology 2023Quote: ... and dapi or NucSpot Live 488 (Biotium) were used for nuclear staining ...
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bioRxiv - Neuroscience 2023Quote: ... along with Lipidspot 488 (Biotium, 70065-T) and imaged for 24 hours.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were incubated in a Flexstation 3 for at least 60 minutes at 37°C in Ca5 dye plus 1.25 mM probenecid (Biotium #50027, Jomar Life Research, Australia) prior to the start of recording ...
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bioRxiv - Neuroscience 2023Quote: ... washed and then treated with TrueBlack lipofuscin autofluorescence quencher (Biotium, product number 23007) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Cellular mitochondrial mass and potential were determined using commercially available probes MitoView Green (MTV-G; Biotium, CA, USA) and Mitotracker Deep Red (MTDR ...
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Antigen Geometry Tunes Mast Cell Signaling Through Distinct FcεRI Aggregation and Structural ChangesbioRxiv - Biophysics 2023Quote: ... and CF640R- (Biotium #92108) labeled IgE lots were prepared using NHS Ester conjugation ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using EvaGreen (Biotium, Catalog no: 89138-982) and the Biorad CFX Connect Real-time PCR system ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with Hoechst 33342 (Biotium; 40046) at 1:10,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Cell boundaries were stained with Phalloidin cf532 in PBS (1:500, Biotium, 00051). Samples were mounted in ProLong Glass Antifade mounting medium (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were mounted in EverBrite TrueBlack® Hardset Mounting Medium (Biotium: 23017). Slides were imaged using Leica Bmi8 Thunder Imager microscope.
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bioRxiv - Biophysics 2023Quote: ... Following staining with GelRed (Biotium) gels were imaged using Gel Doc EZ gel system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in 4% paraformaldehyde (PFA) in PBS (Biotium 22023) for 15 minutes at room temperature followed by pre-cooled (-20°C ...
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bioRxiv - Cell Biology 2023Quote: ... and donkey anti-mouse CF583R (Biotium catalog: 20794-500UL), were used as secondaries for lamp1 (rabbit anti-lamp1 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by donkey anti-mouse CF583R (Biotium catalog: 20794-500UL). Three-color STORM used the following labeling scheme ...
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bioRxiv - Cell Biology 2023Quote: ... the IVT reaction was doped with 5-amino-allyl UTP (Biotium) at 1:10 (aminoallyl-UTP ...
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bioRxiv - Genomics 2023Quote: ... 1x evagreen (Biotium, 31000T), 0.25 uM dialout primers (see Table 1) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were stained with 1 μM Mito View Green solution (Biotium) and DAPI on a glass slide for 30 min and 5 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... High-molecular weight genomic DNA was confirmed from these extractions by visualizing each isolation on a 1% agarose gel stained with GelGreen® nucleic acid stain (Biotium, Hayward, CA, USA). Isolation quantity and quality was assessed using a Qubit™ fluorometer and a NanoDrop™ One (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Japan) for 2 h at room temperature and then stained with 1 μM Mito View Green solution (Biotium, Fremont, CA) and 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Genetics 2023Quote: ... DAPI (Biotium, BT-40043) was used at 0.1 μg/ml.
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bioRxiv - Genetics 2023Quote: ... adding DAPI 10 µg/ml (Biotium, BT-40043) in the last wash ...
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bioRxiv - Molecular Biology 2023Quote: ... MTSEA-biotin (N-biotinylaminoethyl-methanethiosulfonate, Biotium) (500 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... Incubation with PMAxx was followed by blue light exposition for 15 min using a Blue LED Device (PMA-Lite™ LED Photolysis Device Biotium #E90002). After blue light exposition ...
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bioRxiv - Molecular Biology 2023Quote: ... purification of tachyzoites from cell debris by filtration though 3 µm filters and incubation of 2×105 extracellular parasites in 39µl of D10 with or without 30µM PMAxx (Biotium) for 15 min in shaking and darkness ...
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bioRxiv - Neuroscience 2023Quote: ... or CF640R (Biotium) as previously described (47) ...
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bioRxiv - Neuroscience 2023Quote: ... (Biotium, 80023) were stereotaxically injected at 50 nl/min into the ventral nucleus accumbens (from bregma ...
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bioRxiv - Molecular Biology 2023Quote: ... The polyacrylamide gels were stained for 45 minutes in a solution of 10X GelRed (Biotium) and thereafter imaged with GelDoc™ XR+ (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... their positivity for RedDot1 (Viability dye, Far-red DNA intercalating agent, Biotium, #40060-1, λEx/λEm (with DNA) = 662/694 nm) ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 μL Fast Evagreen qPCR Master Mix (Biotium, 31003), 2 μL of nuclease-free water ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated with DAPI for 30 seconds before coverslipping with EverBrite (Biotium, 23003) hardset mounting medium and drying overnight at room temperature [32] ...
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bioRxiv - Molecular Biology 2023Quote: ... and Gelred (Biotium). Exon skipping was quantified using the DNF-910 dsDNA analysis kit (35-1500 bp ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of each fecal inoculum was mixed with 2.5 µL of 20 mM propidium monoazide (PMA) dye (Biotium, Fremont, CA, USA). The samples were then incubated in the dark for 5 min ...